Nano magnetic-based ELISA and nano magnetic-based latex agglutination test for diagnosis of experimental trichinellosis.

Human trichinellosis is a worldwide foodborne public health threat. Detecting circulating antigens of Trichinella spiralis "T. spiralis" allows for an early diagnosis before larval encystation develops in skeletal muscles. For the first time, the present study aimed to formulate an effective nanomagnetic beads based-ELISA and -latex agglutination test (NMB-ELISA and NMB-LAT) to recognize T. spiralis adult worm crude extract antigen (AWCEA) in sera of experimentally infected mice. The study included thirty-eight mice classified into 3 groups; T. spiralis-infected group (GI) which was euthanized 6, 8, 10, 12, 14 days post-infection (dpi), other parasitic infections group (GII) and healthy control group (GIII). Rabbit anti-T. spiralis polyclonal antibodies (pAbs) were utilized to detect AWCEA in serum samples by sandwich ELISA, NMB-ELISA, and NMB-LAT. Using NMB-ELISA, AWCEA was detected in sera collected at 6 and 8 dpi, with a sensitivity of 50% and 75%, respectively, and a specificity of 100%. Whereas, sandwich ELISA and NMB-LAT couldn't detect the antigen at the same time intervals. Both ELISA formats were able to detect the antigen in samples collected at 10, 12, and 14 dpi with a sensitivity of 100% for NMB-ELISA and 25%, 75%, and 100% respectively, for sandwich-ELISA. Yet, NMB-LAT couldn't detect AWCEA until 12 dpi with a sensitivity of 50% and specificity of 75%. In conclusion, NMB-ELISA is a promising sensitive tool for early and specific diagnosis of acute trichinellosis. The use of NMB-LAT could be a helpful screening procedure in field surveys.

Insecticidal, Oxidative, and Genotoxic Activities of Syzygium aromaticum and Eucalyptus globulus on Culex pipiens Adults and Larvae.

OBJECTIVE: The wide-reaching Culex pipiens has long been a public apprehension. Combating serious vector-borne diseases requires the use of insecticides effective against both humans and the ecosystem. The wide variation of botanicals that nature has to offer tempts researchers to study their interactions with the insects. Environment-friendly insecticides light up hope for maintaining ecological balance and pollution mitigation. This study aimed at evaluating the insecticidal, oxidative, and genotoxic activities of eucalyptus and clove oils on C. pipiens adults and larvae. METHODS: The chemical composition of essential oils was determined via gas chromatography/mass spectrometry. The bioassay was performed, with eucalyptus oil showing the highest toxicity index (LC50 of 0.108% after 24 h in adults and LC50 of 0.014% after 48 h in larvae). RESULTS: Fumigation effects showed Eucalyptus to have higher toxicity than clove oil, with an LC50 of 0.108% and 0.014% after 24 h and 48 h, respectively, in adults and larvae. The effect of tested oils on the activities of glutathione peroxidase, catalase, and superoxide dismutase varied with increasing oil concentrations. The genotoxic effects of the tested oils were dose-dependent, with an increase of all comet parameters compared with those in the control. CONCLUSION: The tested oils showed encouraging potentiality as green insecticides in combating C. pipiens.

Bioefficacy of Some Egyptian Aromatic Plants on Culex pipiens (Diptera: Culicidae) Adults and Larvae.

BACKGROUND: Protecting the environment from chemical hazards of synthetic insecticides along with offering of new breeding areas for vectors by urbanization indicate the trial of natural insecticides. METHODS: The acetone extracts of Anethum graveolens, Ocimum basilicum and Thymus vulgaris were tested for their insecticidal effect on Culex pipiens adults and larvae in different concentrations depending on the technique used. RESULTS: The extracts were significantly effective in all models used with basil being the best in all tested three techniques (LC50= 0.064) in larval feeding, (LC50= 0.330) in CDC bottle assay and (LC50= 13.148) in adults feeding (P< 0.05). CONCLUSION: The results recommend the eco-friendly studied extracts as candidates for controlling Cx. pipiens the lymphatic filariasis vector.

Musca domestica laboratory susceptibility to three ethnobotanical culinary plants.

Throughout history, synanthropic Musca domestica had remained a worldwide problem whenever poor sanitation and bad hygienic conditions exists. Houseflies growing resistance to chemical insecticides are a rising environmental problem that necessitates search for alternatives. Mentha cervina, Ocimum basilicum, and Coriandrum sativum were tested for bioactivity on M. domestica adults and larvae. They are culinary Mediterranean plants. In adulticidal bioassay, using both CDC bottles and fumigation techniques, basil was the most effective extract with LC50 1.074 and 34.996 g/L, respectively. Concerning larvicidal bioassay by fumigation technique, coriander had the highest toxicity index with LC50 29.521 g/L. In both dipping and feeding technique, basil had the highest toxicity with LC50 32.643 and 0.749 g/L, respectively. Basil showed the highest toxicity results in four out of the five models tested followed by coriander then mint; this result highlights the potentiality of basil as a green insecticide in management of flies and opens new insight in the industrialization of basil-based fly control products.

Potential correlation between carboxylic acid metabolites in Biomphalaria alexandrina snails after exposure to Schistosoma mansoni infection.

Carboxylic acids play an important role in both aerobic and anaerobic metabolic pathways of both the snail and the parasite. Monitoring the effects of infection by schistosome on Biomphalaria alexandrina carboxylic acids metabolic profiles represents a promising additional source of information about the state of metabolic system. We separated and quantified pyruvic, fumaric, malic, oxalic, and acetic acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to detect correlations between these acids in both hemolymph and digestive gland gonad complex (DGG's) samples in a total of 300 B. alexandrina snails (150 infected and 150 controls) at different stages of infection. The results showed that the majority of metabolite pairs did not show significant correlations. However, some high correlations were found between the studied acids within the control group but not in other groups. More striking was the existence of reversed correlations between the same acids at different stages of infection. Some possible explanations of the underlying mechanisms were discussed. Ultimately, however, further data are required for resolving the responsible regulatory events. These findings highlight the potential of metabolomics as a novel approach for fundamental investigations of host-pathogen interactions as well as disease surveillance and control.

Carboxylic acids as biomarkers of Biomphalaria alexandrina snails infected with Schistosoma mansoni.

Biomphalaria alexandrina snails play an indispensable role in transmission of schistosomiasis. Infection rates in field populations of snails are routinely determined by cercarial shedding neglecting prepatent snail infections, because of lack of a suitable method for diagnosis. The present study aimed at separation and quantification of oxalic, malic, acetic, pyruvic, and fumaric acids using ion-suppression reversed-phase high performance liquid chromatography (HPLC) to test the potentiality of these acids to be used as diagnostic and therapeutic biomarkers. The assay was done in both hemolymph and digestive gland-gonad complex (DGG) samples in a total of 300 B. alexandrina snails. All of the studied acids in both the hemolymph and tissue samples except for the fumaric acid in hemolymph appeared to be good diagnostic biomarkers as they provide not only a good discrimination between the infected snails from the control but also between the studied stages of infection from each other. The most sensitive discriminating acid was malic acid in hemolymph samples as it showed the highest F-ratio. Using the Z-score, malic acid was found to be a good potential therapeutic biomarker in the prepatency stage, oxalic acid and acetic acid in the stage of patency, and malic acid and acetic acid at 2 weeks after patency. Quantification of carboxylic acids, using HPLC strategy, was fast, easy, and accurate in prediction of infected and uninfected snails and possibly to detect the stage of infection. It seems also useful for detection of the most suitable acids to be used as drug targets.