RESUMO
A chemometric approach has been used to optimize the Agilent multimode ion source. Initial factorial experimental design studies indicated that there was a significant degree of curvature in the experimental region, so further central composite design experiments were performed. Optimum conditions were found using statistical optimization tools, and these results were then validated. As a result, recommendations have been made for the value of each operational parameter in order to optimize response.
Assuntos
Biotecnologia/métodos , Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Preparações Farmacêuticas/análise , Adsorção , Anti-Inflamatórios não Esteroides/análise , Anti-Inflamatórios não Esteroides/química , Biotecnologia/instrumentação , Cromatografia Líquida/instrumentação , Ibuprofeno/análise , Ibuprofeno/química , Íons , Espectrometria de Massas/instrumentação , Preparações Farmacêuticas/química , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Solubilidade , Solventes/química , TemperaturaRESUMO
The use of high salt solution to precipitate RNA in a pharmaceutical-grade plasmid DNA purification process was investigated. Five antichaotropic salts were tested for their potential to precipitate RNA. Calcium chloride was by far the best precipitant with high RNA removal in a very short incubation time. Calcium chloride precipitation conditions were investigated at two stages of a plasmid purification process using experimental design techniques. The effect of up to five factors on RNA precipitation and plasmid recovery was assessed by statistical modeling. Optimized conditions for calcium chloride precipitation were then introduced to the plasmid purification process resulting in the efficient removal of most impurities (RNA, chromosomal DNA, proteins, and endotoxins).
