RESUMO
Humoral and cell-mediated immune responses were studied in forty scabietic patients and twenty healthy control individuals. The parameter findings for quantitative assessment of humoral immune response by the single radial immune diffusion technique indicated that IgA was significantly decreased, IgG & IgM were significantly increased while there was no statistical difference in the level of IgE, C3 & C4 compared to the normal control group. The cell mediated immune response was estimated in vivo by the intradermal test. It showed insignificant decrease in the number of positive reactors versus the control group. The macrophage migration inhibition test as an in vitro index for determining the cell mediated immune response was significantly diminished. This study indicates that humoral and cell-mediated immune responses play an important role in the pathogenesis of scabies.
Assuntos
Imunoglobulinas/biossíntese , Escabiose/imunologia , Estudos de Casos e Controles , Complemento C3/análise , Complemento C4/análise , Humanos , Imunidade CelularRESUMO
A study has been done to find the effect of purification of crude antigens, extracted from adult Fasciola gigantica and Fasciola hepatica worms on the sensitivity of some serological techniques in diagnosis of fascioliasis. Using C.I.E.P. test, the partially purified antigens of both Fasciola species were less sensitive than their corresponding crude ones. Using I.H.A., the difference between results of crude and partially purified antigens for the two species were statistically insignificant, both (P greater than 0.05). So in applying this test, there is no need for the partially purification of antigen. On using E.L.I.S.A. the partially purified antigens showed higher sensitivities for detection of fascioliasis than their corresponding crude ones. Partially purified F. gigantica and F. hepatica antigens gave nearly the same results whether using C.I.E.P., I.H.A. or E.L.I.S.A. However, E.L.I.S.A. was the most sensitive technique followed by I.H.A. then C.I.E.P.