Comparison of quantitative variables between patients with relatively normal and gastritis-altered mucosae.

OBJECTIVE: To develop a statistical (regression) analysis of quantitative patterns of human epithelial nuclear and cellular profiles from chronic atrophic and hypertrophic gastritis, excluding Menetrier's gastropathy. STUDY DESIGN: The study group consisted of 30 patients with chronic gastritis with or without intestinal metaplasia, including gastric dysplasia. The control group consisted of 25 patients with relatively normal mucosae. Both groups were compared with respect to changes in quantitative morphometric variables as well as nuclear DNA quantities. RESULTS: Specific interdependences between minor (BN) and major (LN) nuclear axis length and cellular caliper diameters (Bc) and (Lc) were stipulated; thus, the corresponding area features were determined by regression equations. The value of the L2 norm as differences between corresponding linear functions involved in the regression equations were also examined. Higher nuclear DNA content values were obtained from patients with chronic gastritis. CONCLUSION: Chronic gastritis appears to be an intermediate and crucial moment in the process of gastric mucosal malignant progression. The pentaploid (5C) region was found to be an intermediate and critical feature of chronic gastritis since values in the ploidy range higher than pentaploid have been found to potentially indicate a malignancy.

Cytophotometric measurements of nuclear DNA content values in human breast lesions.

Quantitative measurements of nuclear DNA content values have been evaluated in cytological smears from 33 women with normal breast, fibrocystic disease, fibroadenoma and different types of breast carcinomas. The DNA amount for normal breast was found to be in the diploid region. For fibrocystic disease and fibroadenoma the cytochemical data were established to range from diploid to tetraploid regions, with peaks at 2 C and 3 C, respectively. For the different types of malignant breast lesions the quantitative DNA amounts were presented in the various regions of the histograms: from diploid to tetraploid for papillar carcinomas and metaplastic carcinomas--fusiformcellular and epidermoid types, with peaks at 2 C; from diploid to octaploid for intracanalicular carcinomas with peaks at 2 C, 4 C and 6 C. Dynamic changes in the pathway from the normal to cancer have been determined after quantitative cytochemical measurements of various benign and malignant breast lesions.

Comparative cytomorphometric and cytospectrophotometric investigations of gastric lesions.

Comparative cytomorphometric and cytospectrophotometric investigations have been carried out on Hematoxylin-Eosin and Feulgen stained paraffin sections from 150 patients with normal gastric mucosa, chronic atrophic and hypertrophic gastritis, benign and malignantly transformed stomach ulcers and adenomatous polyps and adenocarcinoma of the stomach. The changes in the small and large nucleus (cell) diameters; nucleus, cell and cytoplasma area (volume) and nucleus/cell and nucleus/cytoplasma ratio for area and volume as well as the nuclear DNA content values have been presented in the pathway from normal stomach mucosa to gastric cancer. The presented results give us more information about the dynamics of malignant cell transformation.

Gastric carcinomas and their lymphogeneous metastases in cytomorphometric expression.

By means of an eyepiece-micrometer, cytomorphometric investigations have been carried out on paraffin sections from 20 patients with different types of primary gastric carcinomas and their lymphogeneous metastases.

[Cytomorphometric studies on normal stomach mucosa, precancerous diseases and stomach cancer]. / Zytomorphometrische Untersuchungen bei normaler Magenschleimhaut, präkanzerösen Krankheiten und Magenkrebs.

By means of a graduated microscope eyepiece-micrometer cytomorphometric investigations have been carried out on paraffin sections from 120 patients with normal gastric mucosa, gastritis, gastric ulcer, polyps and carcinoma of the stomach. In normal mucosa and gastritis, the number of the cell groups is low, the curves are predominantly single peaked, shifted to the left and the sizes of the nuclei are small. In gastric ulcer, polyps and carcinoma the number of the cell groups is high, the curves are multi-peaked, without any well expressed peaks, shifted to the right and the sizes of the nuclei are large.