RESUMO
We have analyzed the multi-mode interference effect depending on the wavelength and the polarization states of input beam in a multi-mode Ti:LiNbO(3) waveguide at about 1300 nm region. The transmitted optical signal of a Ti:LiNbO(3) waveguide shows the periodic oscillation as a function of input wavelength. The measured average periodicity of the oscillation in TM and TE polarization beams were about 18 nm and 48 nm, respectively. Actually, the periodicity is determined by the refractive index difference between the two modes (fundamental and first modes). Therefore, we have explained the experimental results with the theoretical calculations which are derived from a quasi-analytical technique based on the effective-refractive- index method and the equation of coupling length determined by the mode phase factor in the multi-mode waveguide.
RESUMO
We have analyzed the Solc filtering characteristics in a periodically poled Ti:LiNbO3 (Ti:PPLN) multimode waveguide. The single- and dual-wavelength filtering were achieved under the optimized guiding condition for the TEM(00)-like mode and two mode (TEM(00)- and TEM(01)-like mode), respectively. The full width at half-maximum of the filter was about 0.21 nm at both guiding conditions. We found that the origin of two peaks of the dual-wavelength Solc filter in the two-mode guiding condition is the different effective refractive index between the TEM(00)- and TEM(01)-like modes. The wavelength difference of two peaks is about 0.8 nm at room temperature.
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In several neuronal systems, nerve growth factor (NGF) and platelet-derived growth factor (PDGF) act as neurogenic agents, whereas epidermal growth factor (EGF) acts as a mitogenic agent. Hippocampal stem cell lines (HiB5) immortalized by the expression of a temperature-sensitive SV40 large T antigen also respond differentially to EGF and PDGF. While EGF treatment at the permissive temperature induces proliferation, the addition of PDGF induces differentiation at the non-permissive temperature. However, the mechanism responsible for these different cellular fates has not been clearly elucidated. In order to clarify possible critical signaling events leading to these distinct cellular outcomes, we examined whether either EGF or PDGF differentially induces the activation of phospholipases, such as phospholipase A(2) (PLA(2)), C (PLC), or D (PLD). Although EGF stimulation did not induce phospholipases, PDGF caused a rapid and transient activation of PLC and PLD, but not PLA(2). When the activation of PLC or PLD was blocked, the neurite outgrowth induced by PDGF was significantly inhibited. Although the activation of PLD occurred faster than PLC, blocking of PLD activity by transient expression of lipase-inactive mutants did not inhibit the induction of PLC activity by PDGF. These results suggest that the differential activation of phospholipases may play an important role in signal transduction by mitogenic EGF and neurotrophic PDGF in HiB5 neuronal hippocampal stem cells. In particular, the activation of phospholipase C and D may contribute to neuronal differentiation by neurogenic PDGF in the HiB5 cells.
Assuntos
Fator de Crescimento Epidérmico/farmacologia , Neurônios/ultraestrutura , Fosfolipases/metabolismo , Fator de Crescimento Derivado de Plaquetas/farmacologia , Células-Tronco/citologia , Fosfolipases Tipo C/metabolismo , Animais , Ácido Araquidônico/farmacocinética , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/fisiologia , Divisão Celular/efeitos dos fármacos , Divisão Celular/fisiologia , Linhagem Celular Transformada , Estrenos/farmacologia , Hipocampo/citologia , Neuritos/efeitos dos fármacos , Neuritos/fisiologia , Neurônios/efeitos dos fármacos , Neurônios/enzimologia , Fenótipo , Inibidores de Fosfodiesterase/farmacologia , Fosfolipase D/metabolismo , Fosfolipases A/metabolismo , Pirrolidinonas/farmacologia , Células-Tronco/efeitos dos fármacos , Células-Tronco/enzimologia , TrítioRESUMO
Neurotrophin regulation of actin-dependent changes in growth cone motility may depend on the signaling of beta-actin mRNA transport. Formation of an RNP complex between the beta-actin mRNA zipcode sequence and Zipcode Binding Protein 1 (ZBP1) was required for its localization to growth cones. Antisense oligonucleotides to the zipcode inhibited formation of this RNP complex in vitro and the neurotrophin-induced localization of beta-actin mRNA and ZBP1 granules. Live cell imaging of neurons transfected with EGFP-ZBP1 revealed fast, bidirectional movements of granules in neurites that were inhibited by antisense treatment, as visualized by FRAP analysis. NT-3 stimulation of beta-actin protein localization was dependent on the 3'UTR and inhibited by antisense treatment. Growth cones exhibited impaired motility in the presense of antisense. These results suggest a novel mechanism to influence growth cone dynamics involving the regulated transport of mRNA.
Assuntos
Actinas/metabolismo , Neurônios/ultraestrutura , Neurotrofina 3/farmacologia , Proteínas de Ligação a RNA/metabolismo , Ribonucleoproteínas/metabolismo , Regiões 3' não Traduzidas , Actinas/análise , Actinas/genética , Animais , Astrócitos , Proteínas Aviárias , Sequência de Bases , Transporte Biológico/efeitos dos fármacos , Células Cultivadas , Embrião de Galinha , Técnicas de Cocultura , Grânulos Citoplasmáticos/química , Imunofluorescência , Expressão Gênica , Hibridização In Situ , Microscopia de Fluorescência , Microtúbulos/química , Dados de Sequência Molecular , Neurônios/química , Oligonucleotídeos Antissenso/farmacologia , Prosencéfalo , RNA Mensageiro/análise , Proteínas de Ligação a RNA/análise , Proteínas de Ligação a RNA/genética , Proteínas Recombinantes de Fusão , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Ribonucleoproteínas/genética , Homologia de Sequência , TransfecçãoRESUMO
Sialyl Lewis X (sLeX) is a tetra-saccharide glycoconjugate of membrane proteins. It acts as a ligand for the selectin proteins during cell adhesion of inflammatory process. Aberrant overexpression of sLeX is also a characteristic of various cancer cells, especially for highly malignant ones. In this paper, the sLeX-specific RNA aptamer was selected using a random RNA library and its affinity and specificity were measured by Surface Plasmon Resonance technique. Affinity of the selected RNA was increased about 1000-fold as compared with the original RNA pool. RNA aptamer bound more specifically to its cognate sugar than to any other similar sugars. Inhibition of the cell adhesion was also shown by in vitro static assay of sLeX-expressing HL60 cells to the E- and P-selectins. It suggests that the high affinity carbohydrate specific RNA aptamer could be used as an alternative to the antibody.
Assuntos
Adesão Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Oligossacarídeos/metabolismo , Sequência de Bases , Técnicas Biossensoriais , Cromatografia de Afinidade , Selectina E/metabolismo , Biblioteca Gênica , Células HL-60 , Humanos , Inflamação , Cinética , Lectinas/metabolismo , Ligantes , Modelos Químicos , Dados de Sequência Molecular , Oligonucleotídeos/química , Selectina-P/metabolismo , Ligação Proteica , Sensibilidade e Especificidade , Antígeno Sialil Lewis X , Ressonância de Plasmônio de SuperfícieRESUMO
The polarization properties of solid-state cube-corner retroreflectors, which are uncoated glass or metal-coated glass on the reflecting surfaces, are examined analytically and compared. Experimental verifications are presented for the case of linearly polarized incident light. When the uncoated cube-corner is used in a heterodyne interferometer, the polarization properties reveal that the axial orientation of the corner reflector has an effect on the strength of the beat signal and the nonlinearity error. A theoretical analysis of the effect is presented together with experimental results.