RESUMO
This study aims to investigate the effects of a proximally-based turnover sheath flap obtained from the tendon sheath on the prevention of adhesion formation in the legs of the chickens whose tendons and sheaths have been injured. The chickens were categorised into 4 groups. Group 1 was chosen as the sham group. In Group 2, a defective area of 0.3 x0.5 cm was created across the sheath, without damaging the tendon. The healthy profundus tendons of the chickens in Group 3 were cut and repaired. Afterwards, a defective area of 0.3 x 0.5 cm was created across the tendon sheath. In Group 4, the healthy profundus tendons were cut and repaired. Then, a defective area of 0.3 x 0.5 cm was created over the reconstructed tendon. This defective area was finally covered with a proximally-based turnover sheath flap of the size 0.4 x 1 cm, which was raised from the distal part of the sheath. The toes were immobilised for 3 weeks before the chickens were sacrificed. Histopathological and biomechanical analyses showed adhesion in the repaired tendons in Group 3, in which movement restriction was shown by biomechanical analyses. The present study showed that covering the defective sheath that was created over the repaired tendon with a proximally-based turnover tendon sheath flap prevented peritendinous adhesion.
Assuntos
Retalhos Cirúrgicos , Traumatismos dos Tendões/cirurgia , Tendões/transplante , Animais , Fenômenos Biomecânicos , Proliferação de Células , Galinhas , Colágeno/análise , Fibroblastos/patologia , Reação a Corpo Estranho/patologia , Traumatismos dos Tendões/patologia , Traumatismos dos Tendões/fisiopatologia , Tendões/patologia , Tendões/fisiopatologia , Aderências Teciduais/prevenção & controle , Cicatrização/fisiologiaRESUMO
The objective of this study was to examine whether a decrease in neutrophil-mediated tissue injury using Fucoidin, a nontoxic neutrophil rolling inhibitor, would improve flap survival in an island flap model after ischemia-reperfusion. Myeloperoxidase activity (an indirect index of tissue neutrophil count) and malondialdehyde (an indicator of lipid peroxidation), the degree of neutrophil infiltration by direct counting, and macroscopic flap survival were assessed in the flap after arterial ischemia-reperfusion. Epigastric island skin flaps were elevated in 56 rats. The first group of 21 rats was subjected to 6 hours of arterial ischemia. The second group of 21 rats was subjected to 10 hours of arterial ischemia, and the rest of the rats were used as nonischemic controls (sham flaps). For inhibiting neutrophil rolling, a nontoxic polysaccharide agent-Fucoidin-was used. Each ischemic group was divided further into three subgroups: Subgroup I (control rats) received saline, subgroup II received 10 mg per kilogram Fucoidin, and subgroup III received 25 mg per kilogram Fucoidin before reperfusion. The results were evaluated as tissue neutrophil counts, tissue malondialdehyde content, tissue myeloperoxidase activity, and flap survival. Neutrophil counts and tissue myeloperoxidase activity were decreased significantly (p <0.001) in subgroup III, but lipid peroxidation by means of tissue malondialdehyde content was not affected by Fucoidin administration. The authors conclude that administration of Fucoidin before reperfusion can limit tissue injury apparently by inhibiting neutrophil rolling in a dose-dependent manner.
Assuntos
Fucose/uso terapêutico , Infiltração de Neutrófilos/efeitos dos fármacos , Polissacarídeos/uso terapêutico , Traumatismo por Reperfusão/prevenção & controle , Retalhos Cirúrgicos/irrigação sanguínea , Animais , Sobrevivência de Enxerto , Malondialdeído/metabolismo , Neutrófilos/patologia , Peroxidase/metabolismo , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Retalhos Cirúrgicos/patologiaRESUMO
The viability of human split thickness skin grafts stored in plasma and saline at +4 degrees C had been evaluated by means of viable keratinocytes by trypan blue method. Skin grafts which were stored in plasma showed better percentage of viable keratinocytes at the end of 30 days than saline. It is concluded that plasma is a better storage medium than saline for preservation of skin grafts.