Vanadium in Bipolar Disorders-Reviving an Old Hypothesis.

Bipolar disorder (BD) is a severe and common chronic mental illness. The biological basis of the disease is poorly understood and its treatment is unsatisfactory. Our previous studies supported the notion that alterations in Na+, K+-ATPase activity were involved in the etiology of BD. As various chemical elements inhibit Na+, K+-ATPase, we determined the concentration of 26 elements in the serum of BD patients before and after treatment and in postmortem brain samples from BD patients, and compared them with matched controls. The only element that was reduced significantly in the serum following treatment was vanadium (V). Furthermore, the concentration of V was significantly lower in the pre-frontal cortex of BD patients compared with that of the controls. Intracerebroventricular administration of V in mice elicited anxiolytic and depressive activities, concomitantly inhibited brain Na+, K+-ATPase activity, and increased extracellular signal-regulated kinase phosphorylation. A hypothesis associating V with BD was set forth decades ago but eventually faded out. Our results are in accord with the hypothesis and advocate for a thorough examination of the possible involvement of chemical elements, V in particular, in BD.

Chemical Element Profiling in the Sera and Brain of Bipolar Disorders Patients and Healthy Controls.

Bipolar Disorder (BD) is a severe recurrent affective mood disorder characterized by a wide range of lifelong mood swings, varying between depressive and manic states. BD affects more than 1% of the world's population irrespective of nationality, ethnic origin, or socioeconomic status and is one of the main causes of disability among young people, leading to cognitive and functional impairment and raised mortality, particularly death by suicide. Trace elements play a vital role in many biochemical and physiological processes. Compelling evidence shows that element toxicity might play a crucial role in the onset and progression of neurodegenerative disorders, but their involvement in mood disorders has been scarcely studied. In the present investigation, we determined the concentration of 26 elements in the serum of BD patients before and after treatment and in postmortem brain samples from BD patients and compared them with matched controls. The only element that was reduced significantly in the serum following treatment was vanadium (V). Furthermore, the concentration of Al, B, Cu, K, Mg and V were significantly lower in the pre-frontal cortex of BD patients compared with those of the controls. A comparison of Spearman's rank correlation coefficients between the elements in the serum and brain of BD patients and control groups pointed to boron and aluminum as being involved in the disease. These results suggest that there is a disturbance in the elements' homeostasis and the inter-elements' relationship in the brain of BD patients and advocate a thorough examination of the possible involvement of chemical elements in different stages of the disease.

Lead in Archeological Human Bones Reflecting Historical Changes in Lead Production.

Forty years ago, in a seminal paper published in Science, Settle and Patterson used archeological and historical data to estimate the rates of worldwide lead production since the discovery of cupellation, approximately 5000 years ago. Here, we record actual lead exposure of a human population by direct measurements of the concentrations of lead in petrous bones of individuals representing approximately 12 000 years of inhabitation in Italy. This documentation of lead pollution throughout human history indicates that, remarkably, much of the estimated dynamics in lead production is replicated in human exposure. Thus, lead pollution in humans has closely followed anthropogenic lead production. This observation raises concerns that the forecasted increase in the production of lead and other metals might affect human health in the near future.

The Genomic History of the Bronze Age Southern Levant.

We report genome-wide DNA data for 73 individuals from five archaeological sites across the Bronze and Iron Ages Southern Levant. These individuals, who share the "Canaanite" material culture, can be modeled as descending from two sources: (1) earlier local Neolithic populations and (2) populations related to the Chalcolithic Zagros or the Bronze Age Caucasus. The non-local contribution increased over time, as evinced by three outliers who can be modeled as descendants of recent migrants. We show evidence that different "Canaanite" groups genetically resemble each other more than other populations. We find that Levant-related modern populations typically have substantial ancestry coming from populations related to the Chalcolithic Zagros and the Bronze Age Southern Levant. These groups also harbor ancestry from sources we cannot fully model with the available data, highlighting the critical role of post-Bronze-Age migrations into the region over the past 3,000 years.

Lead isotopes in silver reveal earliest Phoenician quest for metals in the west Mediterranean.

When and why did the Phoenicians initiate long-term connections between the Levant and western Europe? This is one of the most hotly debated questions in ancient Mediterranean history and cultural research. In this study, we use silver to answer this question, presenting the largest dataset of chemical and isotopic analyses of silver items from silver hoards found in Phoenician homeland sites. Intertwining lead isotope analysis of silver items with precise archaeological context and chronology, we provide analytical evidence for the onset of Phoenician westward expansion. We suggest that the quest for silver instigated a long, exploratory phase, first in Anatolia (Asia Minor) and Sardinia, and subsequently in the Iberian Peninsula. This phase preceded the establishment of sustainable, flourishing Phoenician colonies in the West by over a century. In so doing, our results buttress the "precolonization" theory, accord it a firm chronological framework, and demonstrate that the quest for silver (and probably other metals) was an incentive for Phoenician westward expansion. Furthermore, our results show that the Phoenicians introduced innovative silver production methods to historic Europe.

Changing flood frequencies under opposing late Pleistocene eastern Mediterranean climates.

Floods comprise a dominant hydroclimatic phenomenon in aridlands with significant implications for humans, infrastructure, and landscape evolution worldwide. The study of short-term hydroclimatic variability, such as floods, and its forecasting for episodes of changing climate therefore poses a dominant challenge for the scientific community, and predominantly relies on modeling. Testing the capabilities of climate models to properly describe past and forecast future short-term hydroclimatic phenomena such as floods requires verification against suitable geological archives. However, determining flood frequency during changing climate is rarely achieved, because modern and paleoflood records, especially in arid regions, are often too short or discontinuous. Thus, coeval independent climate reconstructions and paleoflood records are required to further understand the impact of climate change on flood generation. Dead Sea lake levels reflect the mean centennial-millennial hydrological budget in the eastern Mediterranean. In contrast, floods in the large watersheds draining directly into the Dead Sea, are linked to short-term synoptic circulation patterns reflecting hydroclimatic variability. These two very different records are combined in this study to resolve flood frequency during opposing mean climates. Two 700-year-long, seasonally-resolved flood time series constructed from late Pleistocene Dead Sea varved sediments, coeval with significant Dead Sea lake level variations are reported. These series demonstrate that episodes of rising lake levels are characterized by higher frequency of floods, shorter intervals between years of multiple floods, and asignificantly larger number of years that experienced multiple floods. In addition, floods cluster into intervals of intense flooding, characterized by 75% and 20% increased frequency above their respective background frequencies during rising and falling lake-levels, respectively. Mean centennial precipitation in the eastern Mediterranean is therefore coupled with drastic changes in flood frequencies. These drastic changes in flood frequencies are linked to changes in the track, depth, and frequency of mid-latitude eastern Mediterranean cyclones, determining mean climatology resulting in wetter and drier regional climatic episodes.

Carbon Nanotube Based Flow-Through Electrochemical Cell for Electroanalysis.

A flow-through electrode made of a carbon nanotubes (CNT) film deposited on a polytetrafluoroethylene (PTFE) membrane was assembled and employed for the determination of low concentration of copper as a model system by linear sweep anodic stripping voltammetry (LSASV). CNT films with areal mass ranging from 0.12 to 0.72 mg cm-2 were characterized by measurement of sheet resistance, water permeation flux and capacitance. Moreover, CNT with two different sizes and PTFE membrane with two different pore diameters (0.45 and 5.0 µm) were evaluated during the optimization of the electrode. Thick layers made of small CNT exhibited the lowest sheet resistance and the greatest analytical response, whereas thin layers of large CNT had the lowest capacitance and the highest permeation flux. Electrodes made of 0.12 mg cm-2 of large CNT deposited on 5.0 µm PTFE enabled sufficiently high mass transfer and collection efficiency for detecting 64 ppt of Cu(II) within 5 min of deposition and 4.0 mL min-1 flow rate. The analytical response was linear over 4 orders of magnitude (10-9 to 10-5 M) of Cu(II). The excellent performance of the flow-through CNT membrane integrated in a flow cell makes it an appealing approach not only for electroanalysis, but also for the electrochemical treatment of waters, such as the removal of low concentrations of heavy metals and organics.

Effect of Dust Storms on the Atmospheric Microbiome in the Eastern Mediterranean.

We evaluated the impact of Saharan dust storms on the local airborne microbiome in a city in the Eastern Mediterranean area. Samples of particles with diameter less than 10 µm were collected during two spring seasons on both dusty and nondusty days. DNA was extracted, and partial 16S rRNA gene amplicons were sequenced using the Illumina platform. Bioinformatic analysis showed the effect of dust events on the diversity of the atmospheric microbiome. The relative abundance of desert soil-associated bacteria increased during dust events, while the relative abundance of anthropogenic-influenced taxa decreased. Quantitative polymerase chain reaction measurements of selected clinically significant antibiotic resistance genes (ARGs) showed that their relative abundance decreased during dust events. The ARG profiles on dust-free days were similar to those in aerosol collected in a poultry house, suggesting a strong agricultural influence on the local ambient profiles. We conclude that dust storms enrich the ambient airborne microbiome with new soil-derived bacteria that disappear as the dust settles, suggesting that the bacteria are transported attached to the dust particles. Dust storms do not seem to be an important vector for transport of probed ARGs.

The Earliest Lead Object in the Levant.

In the deepest section of a large complex cave in the northern Negev desert, Israel, a bi-conical lead object was found logged onto a wooden shaft. Associated material remains and radiocarbon dating of the shaft place the object within the Late Chalcolithic period, at the late 5th millennium BCE. Based on chemical and lead isotope analysis, we show that this unique object was made of almost pure metallic lead, likely smelted from lead ores originating in the Taurus range in Anatolia. Either the finished object, or the raw material, was brought to the southern Levant, adding another major component to the already-rich Late Chalcolithic metallurgical corpus known to-date. The paper also discusses possible uses of the object, suggesting that it may have been used as a spindle whorl, at least towards its deposition.

Microbial reporters of metal bioavailability.

When attempting to assess the extent and the implications of environmental pollution, it is often essential to quantify not only the total concentration of the studied contaminant but also its bioavailable fraction: higher bioavailability, often correlated with increased mobility, signifies enhanced risk but may also facilitate bioremediation. Genetically engineered microorganisms, tailored to respond by a quantifiable signal to the presence of the target chemical(s), may serve as powerful tools for bioavailability assessment. This review summarizes the current knowledge on such microbial bioreporters designed to assay metal bioavailability. Numerous bacterial metal-sensor strains have been developed over the past 15 years, displaying very high detection sensitivities for a broad spectrum of environmentally significant metal targets. These constructs are based on the use of a relatively small number of gene promoters as the sensing elements, and an even smaller selection of molecular reporter systems; they comprise a potentially useful panel of tools for simple and cost-effective determination of the bioavailability of heavy metals in the environment, and for the quantification of the non-bioavailable fraction of the pollutant. In spite of their inherent advantages, however, these tools have not yet been put to actual use in the evaluation of metal bioavailability in a real environmental remediation scheme. For this to happen, acceptance by regulatory authorities is essential, as is a standardization of assay conditions.

European atmospheric pollution imported by cooler air masses to the Eastern Mediterranean during the summer.

In this study we combine Pb isotopes, Pb, Ni, Cu, Zn, and several major metal concentrations, identification of the aerosol particles, and synoptic and back trajectory analyses to obtain direct evidence for the extent and nature of mixing between Middle Eastern and European sources emissions of metals and aerosols. During the summer months aerosols collected in Israel are highly polluted by metals (EF(Ni) = 120, EF(Cu) = 320, EF(Zn) = 30, EF(Pb) = 540; average values). The fraction of European Pb of mostly industrial sources is 61 +/- 21% in Jerusalem, and the fraction of European Cu, Zn, Ni, and aerosols should be on the same order. Whenever a steep pressure gradient is built between the barometric trough originating from the Persian Gulf and the subtropical ridge along the African coast, stronger westerly winds and cooler temperatures (deep Persian Trough) prevail overthe Middle East, and higher amounts of European pollution are observed in the atmosphere (74 +/- 13%). On the other hand, when the Persian Trough is in its shallow mode, the proportion of European pollution is lower (45 +/- 18%, based on Pb isotopes). This study demonstrates that atmospheric pollution over the East Mediterranean region during the summer is influenced not only by local atmospheric dispersion conditions but also by the ability of the atmosphere to inherit a significant proportion of pollutants from European sources.

Trans boundary transport of pollutants by atmospheric mineral dust.

The transport of anthropogenic pollution by desert dust in the Eastern Mediterranean region was studied by analyzing major and trace element composition, organic species, and Pb isotope ratios in suspended dust samples collected in Jerusalem, Israel. Dust storms in this region are associated with four distinct synoptic conditions (Red Sea Trough (RS), Eastern High (EH), Sharav Cyclone (SC), and Cold Depression (Cyprus low, CD)) that carry dust mostly from North African (SC, CD, EH) and Arabian and Syrian (RS, EH) deserts. Substantial contamination of dust particles by Pb, Cu, Zn, and Ni is observed, while other elements (Na, Ca, Mg, Mn, Sr, Rb, REE, U, and Th) display natural concentrations. Sequential extraction of the abovementioned elements from the dust samples shows that the carbonate and sorbed fractions contain most of the pollution, yet the Al-silicate fraction is also contaminated, implying that soils and sediments in the source terrains of the dust are already polluted. We identified the pollutant sources by using Pb isotopes. It appears that before the beginning of the dust storm, the pollutants in the collected samples are dominated by local sources but with the arrival of dust from North Africa, the proportion of foreign pollutants increases. Organic pollutants exhibit behavior similar and complementary to that of the inorganic tracers, attesting to the importance of anthropogenic-pollutant addition en route of the dust from its remote sources. Pollution of suspended dust is observed under all synoptic conditions, yet it appears that easterly winds carry higher proportions of local pollution and westerly winds carry pollution emitted in the Cairo basin. Therefore, pollution transport by mineral dust should be accounted for in environmental models and in assessing the health-related effects of mineral dust.

Origin of lead in eight Central European peat bogs determined from isotope ratios, strengths, and operation times of regional pollution sources.

Lead originating from coal burning, gasoline burning, and ore smelting was identified in 210Pb-dated profiles through eight peat bogs distributed over an area of 60,000 km2. The Sphagnum-dominated bogs were located mainly in mountainous regions of the Czech Republic bordering with Germany, Austria, and Poland. Basal peat 14C-dated at 11,000 years BP had a relatively high 206Pb/207Pb ratio (1.193). Peat deposited around 1800 AD had a lower 206Pb/207Pb ratio of 1.168-1.178, indicating that environmental lead in Central Europe had been largely affected by human activity (smelting) even before the beginning of the Industrial Revolution. Five of the sites exhibited a nearly constant 206Pb/207Pb ratio (1.175) throughout the 19th century, resembling the "anthropogenic baseline" described in Northern Europe (1.17). At all sites, the 206Pb/207Pb ratio of peat decreased at least until 1980; at four sites, a reversal to more radiogenic values (higher 206Pb/207Pb), typical of easing pollution, was observed in the following decade (1980-1990). A time series of annual outputs for 14 different mining districts dispersing lead into the environment has been constructed for the past 200 years. The production of Ag-Pb, coal, and leaded gasoline peaked in 1900, 1980, and 1980, respectively. In contrast to other European countries, no peak in annual Pb accumulation rates was found in 1900, the year of maximum ore smelting. The highest annual Pb accumulation rates in peat were consistent with the highest Pb emission rates from coal-fired power plants and traffic (1980). Although maximum coal and gasoline production coincided in time, their isotope ratios were unique. The mean measured 206Pb/207Pb ratios of local coal, ores, and gasoline were 1.19, 1.16, and 1.11, respectively. A considerable proportion of coal emissions, relative to gasoline emisions, was responsible for the higher 206Pb/207Pb ratios in the recent atmosphere (1.15) compared to Western Europe (1.10). As in West European countries, the gasoline sold in the Czech Republic during the Communist era (1948-1989) contained an admixture of low-radiogenic Precambrian lead from Australia.

Transboundary atmospheric lead pollution.

A high-temporal resolution collection technique was applied to refine aerosol sampling in Jerusalem, Israel. Using stable lead isotopes, lead concentrations, synoptic data, and atmospheric modeling, we demonstrate that lead detected in the atmosphere of Jerusalem is not only anthropogenic lead of local origin but also lead emitted in other countries. Fifty-seven percent of the collected samples contained a nontrivial fraction of foreign atmospheric lead and had 206Pb/207Pb values which deviated from the local petrol-lead value (206Pb/207Pb = 1.113) by more than two standard deviations (0.016). Foreign 206Pb/207Pb values were recorded in Jerusalem on several occasions. The synoptic conditions on these dates and reported values of the isotopic composition of lead emitted in various countries around Israel suggest that the foreign lead was transported to Jerusalem from Egypt, Turkey, and East Europe. The average concentration of foreign atmospheric lead in Jerusalem was 23 +/- 17 ng/m3, similar to the average concentration of local atmospheric lead, 21 +/- 18 ng/ m3. Hence, the load of foreign atmospheric lead is similar to the load of local atmospheric lead in Jerusalem.

Phytoplankton-mediated redox cycle of iron in the epilimnion of Lake Kinneret.

The biological-mediated redox cycle of Fe was studied in the epilimnion of Lake Kinneret (Sea of Galilee), a mesotrophic lake in Israel. Multi-annual lake water sampling and incubation experiments were carried out to study Fe(III) reduction by natural phytoplankton populations and their possible role in inhibiting Fe(II) oxidation. The reduction characteristics of the dinoflagellate Peridinium gatunense, the dominant lake alga, were further examined in the laboratory. The steady-state concentration of Fe(II) calculated from the assessed reduction and oxidation rates was compared with Fe(II) measured in the lake in order to evaluate the significance of these processes to the lake Fe redox cycle. Nanomolar concentrations of Fe(II) were measured in the oxygenated, high pH, upper water layer of the lake throughout the year. Reduction rates of Fe by natural phytoplankton assemblages ranged between 0.1 and 10 nM/h. The highest reduction rates, determined in dinoflagellate-dominated lake waters, coincided with the highest concentrations of Fe(II) measured simultaneously in the lake. Iron(II) oxidation rates calculated from the measured lake Fe(II) and the obtained reduction rates were significantly slower than published abiotic Fe(II) oxidation rates. Indeed, Fe(II) oxidation rates measured in algal-enriched lake water were 30-fold slowerthan Fe(II) oxidation rates in natural water, demonstrating the potential for Fe(II) stabilization by the lake phytoplankton.