Live birth after transfer of a tripronuclear embryo: An intracytoplasmic sperm injection as a combination of microarray and time-lapse technology.

Although around 1-4% of human zygotes have been found to be tripronuclear, there is little information about the subsequent development and chromosomal composition of embryos that derive from these zygotes. Herein, we report a pregnancy and subsequent delivery of a healthy newborn after the transfer of a blastocyst that developed from a tripronuclear zygote that had a euploid microarray result.

Reproducibility of a time-lapse embryo selection model based on morphokinetic data in a sequential culture media setting.

OBJECTIVE: To compare the outcomes of embryos that were given a dynamic score based on a preconstructed embryo scoring model and to analyze whether this model complies with our data. MATERIAL AND METHODS: A total of 910 transferred embryos with known implantation data were retrospectively analyzed in this study. All of the embryos were given a dynamic score based on the preconstructed hierarchical embryo scoring model. RESULTS: The highest pregnancy rate was seen in groups C+ and A- (48.2% for each), and the lowest was observed in Group E (19.7%). When implantation and clinical pregnancy rates were compared between groups, it was found that the highest and statistically significant implantation and clinical pregnancy rates were seen in group C+ (32.7% for each, p=0.000). They were dropped down to 29.4% in Group A-. CONCLUSION: The outcomes of the embryos based on the dynamic score do not comply with the results of the preconstructed model. Each IVF laboratory is unique based on its practice. Therefore, we suggest that each IVF laboratory should determine its own embryo selection criteria based on its own data instead of using a preconstructed model.

Frequency of embryo multinucleation detected by time-lapse system and its impact on pregnancy outcome.

OBJECTIVE: To compare the detection rate of multinucleation with the time-lapse system and conventional control timing proposed by European Society of Human Reproduction and Embryology (ESHRE) consensus and evaluate its impact on pregnancy rates. DESIGN: Retrospective study. SETTING: A private IVF center. PATIENT(S): A total of 686 embryos from 511 intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): A time-lapse system was used to acquire embryo images until ET; the stored data were reviewed for the presence and persistence of multinucleation. The detection rate of multinucleation was compared with ESHRE/ALPHA consensus-proposed embryo evaluating times (23 ± 1, 26 ± 1, 44 ± 1 hours). Morphokinetic characteristics of multinucleated embryos and the effect of multinucleation on pregnancy rate were researched. RESULT(S): Multinucleation was detected in 159 embryos of 145 ICSI cycles. Using ESHRE/ALPHA consensus embryo evaluating times, only 44 (27.6%) out of 159 multinucleated embryos could be identified. In cycles with multinucleated ETs compared with cycles with no multinucleated embryos, clinical pregnancy rates (respectively, 23.4 vs. 44) and implantation rates (respectively, 23.3 vs. 43.6) were significantly lower. Time to 2-cell, 4-cell, and 6-cell stage was significantly longer in multinucleated embryos. Patient age (odds ratio [OR], 0.95; confidence interval [CI], 0.92-0.98) and presence of multinucleation (OR, 0.37; CI, 0.24-0.56) were the only significant predictors of clinical pregnancy rate. CONCLUSION(S): The time-lapse monitoring system seems to be a valuable tool to identify all cases with multinucleation. We conclude that the detection of multinucleation by time-lapse monitoring is associated with lower implantation and clinical pregnancy rates.