Self-Reported, Work-Related Injuries and Illnesses Among Restaurant Workers in Shiraz City, South of Iran.

BACKGROUND: Restaurant sector is one of the most rapidly developing sectors in the world and there is evidence that restaurant industry has high levels of work-related diseases and injuries. This study examined the prevalence of self-reported work-related injuries and illness (WRIIs) and their association with demographic variables among restaurant workers in Shiraz, the capital of Fars Province, Iran. METHODS: In this cross-sectional study, 300 randomly selected restaurant workers completed a self-statement, research-made questionnaire regarding the prevalence of self-reported WRIIs, in Shiraz, Iran. Statistical analyses were performed using SPSS version 20. RESULTS: A high prevalence of work-related injuries (84%) and musculoskeletal disorders (70%) was reported among restaurant workers. Cuts and lacerations, arising from accidents with knives, were the most common injuries seen, followed by burns, falls, slips and trips. Moreover, the prevalence of occupational accidents had a significant association with work experience (p = 0.012), cooking (p < 0.001), as well as preparation and washing (p = 0.011). Age (p < 0.001) and work experience (p < 0.001) had a significance association with the prevalence of musculoskeletal disorders. CONCLUSION: Preventive measures and polices, through providing occupational health and safety services including trainings, personal protective equipment and health examinations, should be taken as to restaurants and catering industry in Iran.

Optimization of complete RB-5 azo dye decolorization using novel cold-adapted and mesophilic bacterial consortia.

Azo dyes are an important group of recalcitrant xenobiotics, which are difficult to degrade and deteriorate in cold environments. In this study, two microbial consortia consisting of cold-adapted and mesophilic bacteria were developed for effective decolorization of Reactive Black-5 azo dye. These bacteria were isolated from textile wastewater and soil of a cold region. Identification of bacterial isolates using 16s rRNA gene analysis revealed that they belong to genus Pseudoarthrobacter, Gordonia, Stenotrophomonas, and Sphingomonas. Decolorization assay was performed for every strain at dye concentrations of 25, 50 and 100 mg/L and the consortia PsGo consisting of mesophilic bacteria and StSp consisting of cold-adapted bacteria were constructed accordingly. Results showed that the consortia PsGo and StSp were able to decolorize 54 and 34 percent of RB-5 (50 mg/L) during 7 days. To improve the dye removal efficiency of the consortia, several parameters including temperature, pH, carbon and nitrogen sources were optimized. Over longer periods, StSp consortium managed to completely decolorize RB-5 (50 mg/L) at optimized conditions of 25-30 °C, pH 9, and using glucose and NH4H2PO4 as carbon and nitrogen source respectively, whereas PsGo consortium decolorized RB-5 (50 mg/mL) completely at 37 °C, pH 11, and with lactose and NH4H2PO4 used as carbon and nitrogen sources. Kinetic of reactions for StSp and PsGo consortia were found to be 0.05 and 0.13 day-1 respectively, but became 0.71 and 0.9 day-1 after optimization. In general, cold ecosystems are good sources for the isolation of novel bacterial strains with a potential application, especially when used as consortia, in environmental biotechnology such as decolorization of RB-5.