RESUMO
All bacterial strains studied retained the viability and ability to form both mono- and polycultural biofilms under conditions of long-term culturing in artificial seawater at 6°C and without addition of nutrients. Bacillus sp. and Pseudomonas japonica presumably stimulated the growth and reproduction of the pathogenic bacteria Listeria monocytogenes and Yersinia pseudotuberculosis. Preserved cell viability in a monoculture biofilm for a long period without adding a food source can indicate allolysis. At the same time, in a polycultural biofilm, the metabolites secreted by saprotrophic strains can stimulate the growth of L. monocytogenes and Y. pseudotuberculosis.
Assuntos
Biofilmes , Listeria monocytogenes , Yersinia pseudotuberculosis , Yersinia pseudotuberculosis/crescimento & desenvolvimento , Yersinia pseudotuberculosis/fisiologia , Biofilmes/crescimento & desenvolvimento , Listeria monocytogenes/crescimento & desenvolvimento , Listeria monocytogenes/fisiologia , Animais , Água do Mar/microbiologia , Pseudomonas/fisiologia , Pseudomonas/crescimento & desenvolvimento , Pseudomonas/metabolismo , Interações Microbianas/fisiologiaRESUMO
We studied the possibility of formation of mixed-species biofilms by Yersinia pseudotuberculosis and Listeria monocytogenes with marine saprotrophic bacteria Flavobacterium sp. and Micrococcus luteus isolated from the coastal waters of the Sea of Japan in summer. Model experiments showed that Flavobacterium sp. and Micrococcus luteus can form both single- and mixed-species biofilms with the specified pathogenic bacteria thus stimulating their growth. This can contribute to the preservation of the pathogens in the marine environment.