RESUMO
We investigated the interaction of two derivatives of bis (indolyl) methane with bovine serum albumin (BSA) using spectroscopic and molecular docking calculations. Fluorescence quenching measurements revealed that the quenching mechanism was static. FÓ§rster energy transfer measurements, synchronous fluorescence spectroscopy and docking studies demonstrated that both bis(indolyl)methanes bound to the Trp residues of BSA. The docking study confirmed that both bis(indolyl)methanes form hydrogen bonds and van der Waals interactions with BSA. Our molecular docking study indicated that the compounds are located within the binding pocket of subdomains IIB and IB of BSA. Fourier transform infrared spectroscopy demonstrated that both bis(indolyl)methane derivatives can interact with BSA and can affect the secondary structure of BSA.
Assuntos
Indóis/química , Simulação de Acoplamento Molecular , Soroalbumina Bovina/química , Animais , Transferência Ressonante de Energia de Fluorescência , Estrutura Molecular , Espectrometria de Fluorescência , Espectrofotometria InfravermelhoRESUMO
The present study aims to determine the main characteristics of ophidian envenomation in the Souss-Massa-Drâa region in Morocco. It consisted of a descriptive retrospective analysis of all snakebite cases recorded by the Moroccan Poison Control and Pharmacovigilance Center, from 1980 to 2008. According to reported data, this region is the most affected in Morocco with 479 cases, that is 27.6% (17 cases/year). The average age of victims was 26.07 ± 18.28 years. The patients developed clinical signs (55%) dominated by disorders of the digestive system (35.5%), cardiovascular diseases (21.4%), and heart rhythm disorders (16.8%). The lethality and sequelae rates were 12% and 1.9%, respectively.
Assuntos
Mordeduras de Serpentes/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Feminino , Geografia , Humanos , Incidência , Lactente , Masculino , Pessoa de Meia-Idade , Marrocos/epidemiologia , Estações do Ano , Mordeduras de Serpentes/mortalidade , Adulto JovemRESUMO
In vitro experiments using the indirectly stimulated rat phrenic-nerve diaphragm preparation have demonstrated simple summation of the inhibitory actions of vecuronium and atracurium.
Assuntos
Atracúrio/farmacologia , Músculos/efeitos dos fármacos , Brometo de Vecurônio/farmacologia , Animais , Diafragma/efeitos dos fármacos , Sinergismo Farmacológico , Masculino , Nervo Frênico/efeitos dos fármacos , Ratos , Ratos EndogâmicosAssuntos
Canal Anal/cirurgia , Anestesia , Ketamina , Reto/cirurgia , Adolescente , Criança , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Estudos ProspectivosRESUMO
Symptomatic hydrocephalus after subarachnoid haemorrhage seems to result both from mechanical obstruction of arachnoid villi and basilar cisterns and from an inflammatory cellular reaction in the villi. Subarachnoid haemorrhage was induced in rabbits using whole blood injected through an implanted intraventricular needle. Control rabbits receiving intraventricular methyl prednisolone acetate but no blood, developed ventricular dilation significantly more often than untreated controls. Eighty-three per cent of rabbits with untreated experimental subarachnoid haemorrhage developed moderate to severe hydrocephalus. Intramuscular steroid therapy significantly reduced the incidence of hydrocephalus.