RESUMO
CONTEXT: Solanum erianthum D. Don and Solanum macranthum Dunal (Solanaceae) are widely used in traditional medicine. The leaves act as an abortifacient and in particular to treat leucorrhoea, sores, and skin irritations. OBJECTIVE: This study was undertaken to characterize the volatile constituents of the leaf and fruit essential oils of S. erianthum and S. macranthum; their antimicrobial and in vitro cytotoxic bioassay against human breast and prostate tumor cells. METHODS: The volatile oils were obtained by hydrodistillation and analyzed for their constituents by gas chromatography-mass spectrometry (GC-MS). Minimum Inhibitory Concentrations (MIC) were determined using the microbroth dilution technique while the cytotoxic potentials were evaluated using the Cell Titre 96((R)) AQ(ueous) Non-Radioactive Cell Proliferation Assay method. RESULTS: Solanum erianthum essential oils were characterized by the abundance of α-terpinolene (17.8%), α-phellandrene (17.5%), p-cymene (15.7%) and ß-pinene (11.7%) in the leaves; α-humulene (23.1%), humulene epoxide II (20.0%), caryophyllene oxide (16.5%), methyl salicylate (11.8%) and ß-caryophyllene (10.9%) in the fruits. The leaf oil of S. macranthum consisted of (E)-phytol (29.0%), pentadecanal (28.1%) and pentadecane (7.7%) while the major fruit oil constituents were α-humulene (36.5%), ß-caryophyllene (17.8%), ethyl palmitate (9.4%), and methyl salicylate (8.2%). Solanum erianthum leaf volatile oil demonstrated potent inhibitory activity against Hs 578T and PC-3 human breast and prostate tumor cells respectively. In addition, the Solanum essential oils exhibited significant antimicrobial activity (19.5-625 µg/mL) on pathogens employed in the assay. CONCLUSION: The Solanum essential oils possess strong antimicrobial activity in addition to the potent cytotoxic potential of S. erianthum leaf oil against Hs 578T and PC-3 cells.
Assuntos
Antibacterianos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologia , Solanaceae , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Destilação , Feminino , Frutas , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Masculino , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Folhas de Planta , Óleos de Plantas/química , Óleos de Plantas/isolamento & purificação , Plantas Medicinais , Neoplasias da Próstata/patologia , Solanaceae/químicaRESUMO
The in vivo antiplasmodial activity of the ethanol seed extract of Picralima nitida grown particularly for the leaf and seed in Niger Delta region of Nigeria was evaluated in Plasmodium berghei berghei infected mice. Picralima nitida (35-115 mg/kg day) exhibited significant (P<0.05) blood schizonticidal activity both in 4-day early infection test and in established infection with a considerable mean survival time though not comparable to that of the standard drug, chloroquine, 5 mg/kg day. The seed extract possesses significant (P<0.05) antiplasmodial activity which correlate with it reported in vitro activity.
Assuntos
Antimaláricos/farmacologia , Apocynaceae , Cloroquina/farmacologia , Malária/tratamento farmacológico , Fitoterapia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Plasmodium berghei/efeitos dos fármacos , Animais , Antimaláricos/administração & dosagem , Antimaláricos/efeitos adversos , Antimaláricos/uso terapêutico , Cloroquina/uso terapêutico , Relação Dose-Resposta a Droga , Feminino , Dose Letal Mediana , Malária/mortalidade , Masculino , Camundongos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/efeitos adversos , Sementes , Taxa de SobrevidaRESUMO
The seed extract of picralima nitida was screened for hypoglycaemic activity. The hypoglycaemic activity was evaluated in both normoglycemic and alloxan-induced diabetic rats, using glibenclamide as a reference drug. In both normoglycaemic and alloxan diabetic groups, 250 mg kg(-1) of the extract, 5 mg kg(-1) glibenclamide and a mixture of the extract (125 mg kg(-1)) and glibenclamide (2.5 mg kg(-1)) were administered orally, respectively. Blood glucose was measured with a glucometer at 0, 1, 2 and 4 h. In the sub acute group, the same doses as above were administered to diabetic rats once daily for 14 days and blood glucose was determined on days 1, 5, 10 and 15. The seed extract did not show any hypoglycaemic effect in both normoglycaemic and alloxan diabetic rats. Rather, the extract increased blood glucose concentration in the sub acute group on days 5, 10 and 15. These results indicate that, unlike the fruit pulp, the seed extract of Picralima nitida did not possess hypoglycaemic activity.
Assuntos
Apocynaceae/química , Hipoglicemiantes/farmacologia , Extratos Vegetais/farmacologia , Sementes/química , Aloxano , Animais , Apocynaceae/embriologia , Glicemia/análise , Diabetes Mellitus Experimental/sangue , RatosRESUMO
Malaria can cause severe disease in pregnancy. We determined asymptomatic parasitaemia in 477 consenting consecutive women during their booking visits to a primary health care facility in a peri-urban area in Lagos State. There were 129 primigravidae and 348 multigravidae, with mean ages of 19.8 +/- 2.4 years (range 16-26 years) and 25.7 +/- 3.7 years (range 19-41 years), respectively. Most of the patients, 77.5% of primigravidae and 73.6% of multigravidae booked in the second trimester. Many (79.1%) of the primigravidae and 81.9% of the multigravidae had not taken any form of antimalaria chemoprophylaxis at the time of booking. Parasitaemia was significantly higher in the primigravidae than in the multigravidae 44.2% vs 33.6% (chi2 = 3.89, P < 0.05). In both primigravidae and multigravidae parasitaemia was highest in the second trimester compared with the two other trimesters (chi2 = 7.92, P < 0.02 and chi2 = 8.54, P < 0.01, respectively). There was no significant difference in parasitaemia among those who had anti-malaria chemoprophylaxis prior to booking and those who did not (P > 0.05). Prevalence of anaemia (PCV < 33%) was high, 73.6% in primigravidae and 69.8% in multigravidae, severe anaemia (PCV < 21%) however, was significantly associated with parasitaemia in the primigravidae (chi2 = 115, P < 0.001). Asymptomatic malaria parasitaemia at booking is high, hence we recommend parasites clearance in all patients at booking.
Assuntos
Malária/epidemiologia , Parasitemia/epidemiologia , Complicações Parasitárias na Gravidez/epidemiologia , Adolescente , Adulto , Distribuição de Qui-Quadrado , Feminino , Humanos , Nigéria/epidemiologia , Paridade , Gravidez , Prevalência , Atenção Primária à Saúde , Saúde SuburbanaRESUMO
The semen quality of 21 Medical students who produced sperm by masturbation has been assessed. The mean density of 21 samples examined on the first day was 64,4 millions per millilitre, while that on the third and fifth day of the study period were 52.2 and 50.7 millilitre respectively. This showed a statistically significant difference between the first and the third samples. Another statistically significant difference (P less than 0.5) was demonstrated in the semen volume of the first and third, and the third and fifth samples respectively. Significant differences were, however, not observed in the motility and morphology changes in the group samples. We therefore conclude that even though repeated semen ejaculations on alternate days do affect the density and volume of the semen, the most important overall effect is not such as to impair the potential fertility of the man who has normal initial sperm characteristics.
Assuntos
Ejaculação , Espermatozoides , Adulto , Humanos , Masculino , Sêmen , Contagem de Espermatozoides , Motilidade dos Espermatozoides , Espermatozoides/anormalidadesRESUMO
Treatment of tinidazole with a mixture of hydrogen peroxide and acetic acid, or liver homogenate preparations, yields the N-3 oxide. This was identified by thin-layer chromatographic analysis on silica gel G, Rf 0.6, using ethanol-chloroform-ammonia (50:49:1) as solvent, and by chemical reduction with sulphur dioxide. UV spectrophotometry and high performance liquid chromatography (HPLC) gave an RT of 0.55 min using Pye Unicam apparatus equipped with a UV detector at 330 nm, a reversed-phase RP 18 (10 microns) column which was 12.5 cm long, a mobile phase of methanol-0.005 M KH2PO4 (pH 4) (20:80, v/v) and a flow rate of 2 ml/min. In-vitro metabolic N-oxidation was achieved by incubating the parent drug, tinidazole, with rat liver homogenates fortified with cofactors at 37 degrees C. HPLC analysis of blood and urine samples from healthy volunteer subjects who took a single oral dose of tinidazole showed the presence of an in-vivo N-oxidation metabolite of the drug. The identical physico-chemical characteristics of the synthetic and biologically produced tinidazole N-oxide strongly suggest that tinidazole, a tertiary amine drug, undergoes metabolic N-oxidation.
Assuntos
Nitroimidazóis/metabolismo , Oxirredução , Tinidazol/metabolismo , Adulto , Fenômenos Químicos , Química , Cromatografia Líquida de Alta Pressão , Humanos , Óxidos/metabolismo , Espectrofotometria Ultravioleta , Tinidazol/sangue , Tinidazol/urinaRESUMO
The use of saliva chloroquine concentrations measurement as a noninvasive technique in the evaluation of the pharmacokinetics of the drug was investigated. Chloroquine concentrations in saliva and plasma were measured in eight healthy volunteers after a single oral dose of two tablets of chloroquine sulfate. The saliva: total plasma concentrations (S/P) ratio was found to be approximately constant in the absorption (0.4 +/- 0.07), distribution (0.47 +/- 0.08), and elimination (0.46 +/- 0.05) phases. Thus, saliva sampling for chloroquine concentrations was found to be a useful noninvasive technique for the estimation of all the pharmacokinetic parameters of the drug and hence, for chloroquine pharmacokinetic studies.
Assuntos
Cloroquina/análise , Saliva/análise , Adulto , Cloroquina/sangue , Cloroquina/farmacocinética , Cromatografia Líquida de Alta Pressão , Ensaios Clínicos como Assunto , Feminino , Humanos , Masculino , Fatores de TempoRESUMO
Eight healthy volunteers who had not taken chloroquine 2 to 12 months previously participated in a single dose study designed to evaluate the pharmacokinetics of chloroquine and some of its metabolites. Each subject received two tablets of chloroquine sulfate (300 mg base) only. Blood and urine samples were collected just before and periodically after chloroquine administration. These samples were assayed for chloroquine and its N-dealkylated metabolites (monodesethylchloroquine, didesethylchloroquine, 7-chloro-4-aminoquinoline), chloroquine side chain N-oxide and chloroquine di-N-oxide using a high performance liquid chromatographic method. Residual levels of chloroquine and its N-oxidation metabolites were found in all subjects. 7-chloro-4-aminoquinoline was eliminated more slowly (t1/2z = 126.48 +/- 20.13 h) than the other metabolites and the unchanged drug (t1/2z = 106.43 +/- 10.13 h). Also, 7-chloro-4-aminoquinoline had a significantly faster (Student's t-test, P less than 0.05) formation clearance when compared with the other metabolites. The plasma concentration of 7-chloro-4-aminoquinoline was about twice that of the unchanged drug while the plasma concentration of monodesethylchloroquine was about 46% that of the unchanged drug. In order to investigate whether the metabolites were produced from the same binding sites or closely related sites on the cytochrome P-450 system, their formation clearances were correlated. The best correlation (r2 = 0.83) was observed for didesethylchloroquine and monodesethylchloroquine, and a fair correlation (r2 = 0.59) was observed for monodesethylchloroquine and 7-chloro-4-aminoquinoline. Formation clearances of the other metabolites were poorly correlated.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Cloroquina/farmacocinética , Administração Oral , Adulto , Biotransformação , Cloroquina/metabolismo , Cromatografia Líquida de Alta Pressão , Remoção de Radical Alquila , Feminino , Humanos , Masculino , OxirreduçãoRESUMO
One-thousand out of a total obstetric population of 3,548 patients seen between January and December, 1985, were screened for asymptomatic bacteriuria. During the first trimester, the observed incidence was 5.3 per cent while in the second and third trimesters it was 4.1 and 2.8 per cent, respectively. The overall pick-up rate was 4.0 per cent. The significance of this observation was discussed. Escherichia coli was the predominant causative micro-organism, accounting for 45 per cent of cases. A seven-day sensitive antibiotic therapy eradicated all the positive cases. The qualitative culture method as described in this study is a simple, sensitive and reliable method of screening for bacteriuria in pregnancy, especially as it can be used for identification of the causative organism and its antibiotic sensitivity.
Assuntos
Bacteriúria/epidemiologia , Escherichia coli/isolamento & purificação , Klebsiella/isolamento & purificação , Complicações Infecciosas na Gravidez/epidemiologia , Staphylococcus aureus/isolamento & purificação , Feminino , Humanos , Nigéria , Gravidez , Complicações Infecciosas na Gravidez/microbiologia , Complicações Infecciosas na Gravidez/urinaRESUMO
Twenty-one healthy Nigerian volunteers distributed into four groups participated in a study to determine the significance of chloroquine disposition in chloroquine-induced pruritus. It involved the administration of chloroquine with or without promethazine pre-administration to the subjects. Group I consisted of 8 chloroquine non-hypersensitive subjects receiving 2 tablets of chloroquine sulphate (300 mg base); Group II consisted of 5 chloroquine non-hypersensitive subjects receiving 2 tablets of chloroquine sulphate 30 minutes after 25 mg promethazine tablet pre-administration; Group III consisted of 5 chloroquine hypersensitive subjects treated as in Group II; Group IV consisted of 3 hypersensitive subjects treated as in Group I. Blood (5 ml) and urine samples were collected periodically for up to 6 days post-dose. The samples were analysed for chloroquine and some of its oxidation metabolites by a specific HPLC method. Probit plots of cumulative drug/metabolite ratios were done to determine if there is polymorphism in chloroquine metabolism. There was bimodality only in the distribution of chloroquine/monodesethylchloroquine ratios, suggesting polymorphism in the metabolic oxidation of chloroquine in these subjects. Higher levels of monodesethylchloroquine were obtained in Group IV subjects when compared with any of the other groups. The oral clearance rate, elimination half-life, and volume distribution at steady state of chloroquine in the study groups were not significantly different (P greater than 0.05). In the absence of promethazine there appears to be an extensive metabolism of chloroquine in hypersensitive individuals to produce monodesethylchloroquine which probably determines the degree of pruritus experienced by an individual.
Assuntos
Cloroquina/farmacocinética , Hipersensibilidade a Drogas/metabolismo , Prurido/metabolismo , Adulto , Cromatografia Líquida de Alta Pressão , Feminino , Meia-Vida , Humanos , Masculino , Prometazina/farmacologia , Prurido/induzido quimicamenteRESUMO
The passage of chloroquine into semen was investigated in four healthy men following the oral administration of four chloroquine sulphate tablets (600 mg base) to each subject. Chloroquine was found to be excreted into semen with a slow transfer rate constant of 0.0002 min-1, and the semen/plasma ratio based on regression analysis was 0.40 +/- 0.06 (mean +/- s.d.). It is concluded that the passage of chloroquine from plasma to semen occurs by passive diffusion.
Assuntos
Cloroquina/metabolismo , Sêmen/metabolismo , Adulto , Cloroquina/sangue , Cloroquina/farmacocinética , Cromatografia em Camada Fina , Difusão , Meia-Vida , Humanos , Concentração de Íons de Hidrogênio , Masculino , Saliva/metabolismoRESUMO
A 30-year-old woman underwent laparotomy and was placed on a seven-day course of metronidazole and ampicillin postoperatively. Chloroquine therapy for malaria was instituted on the sixth day and the patient developed acute dystonic reactions after a single dose. Diphenhydramine therapy before chloroquine administration did not prevent the development of the dystonic reactions. The extrapyramidal symptoms subsided upon diazepam administration and chloroquine withdrawal even though metronidazole therapy was continued. The mechanism of this adverse drug reaction based on the pharmacodynamic interaction between chloroquine and metronidazole is discussed. It is suggested that the combination of pyrimethamine and sulfadoxine be used in place of chloroquine for malaria chemotherapy in patients on metronidazole therapy.
Assuntos
Cloroquina/efeitos adversos , Distonia/induzido quimicamente , Metronidazol/efeitos adversos , Adulto , Doenças dos Gânglios da Base/induzido quimicamente , Doenças dos Gânglios da Base/tratamento farmacológico , Diazepam/uso terapêutico , Interações Medicamentosas , Distonia/fisiopatologia , Feminino , HumanosRESUMO
A controlled study was carried out in ten healthy, male volunteers (randomly distributed into control and test groups of five subjects each) to determine the effect of low-dose cimetidine on chloroquine elimination. The control group subjects received two tablets of chloroquine sulfate (300-mg base) only, while the test group subjects took 400-mg cimetidine at bedtime for four days prior to chloroquine (two tablets of chloroquine sulfate) administration and throughout the duration of the study. Blood samples, 5 mL, were collected periodically after chloroquine administration. The samples were assayed for chloroquine and monodesethylchloroquine using a combination of thin-layer chromatography and spectrophotometry. Wilcoxon's test for unpaired data at P less than .05 was used to determine if there was any significant difference in the elimination of chloroquine in the test group when compared with the control group. The apparent oral clearance rate of chloroquine was reduced from 0.49 +/- 0.04 L/d/kg in the control group to 0.23 +/- 0.02 L/d/kg in the test group, and the elimination half-life was prolonged from 3.11 days in the control group to 4.62 days in the test group. There was a 47.04% reduction in the AUC0-7d of monodesethylchloroquine, the major metabolite of chloroquine, in the test group when compared with the control group. The apparent volume of distribution at steady state was increased from 0.46 +/- 0.07 L/kg in the control group to 0.72 +/- 0.10 L/kg in the test group. All these changes were statistically significant. The conclusion is that cimetidine impairs the elimination of chloroquine in healthy subjects.
Assuntos
Cloroquina/farmacocinética , Cimetidina/farmacologia , Adulto , Cloroquina/urina , Ensaios Clínicos como Assunto , Interações Medicamentosas , Humanos , MasculinoRESUMO
Metronidazole when treated at the N-3 nitrogen with a mixture of hydrogen peroxide and acetic acid, or liver homogenate preparations, yields the N-3 oxide as identified by thin-layer chromatographic analysis on silica gel G, RF 0.62 in ethanol-chloroform-ammonia (50:49:1), by chemical reduction with sulphur dioxide, and by ultra-violet spectrophotometry and nuclear magnetic resonance spectroscopy. Incubation of metronidazole at 37 degrees C with rat liver 10,000g supernatant fortified with cofactors gave a product with identical chromatographic and UV spectral data suggesting that metronidazole like other tertiary amine drugs undergoes microsomal N-oxidation.
Assuntos
Metronidazol/metabolismo , Animais , Cromatografia em Camada Fina , Técnicas In Vitro , Espectroscopia de Ressonância Magnética , Oxirredução , Ratos , Ratos Endogâmicos , Espectrofotometria UltravioletaRESUMO
Ten healthy, male volunteers (aged 19-27 yr; weight 62-67 kg) were randomly distributed into control and test groups of five subjects each in a controlled study on the effect of ranitidine on chloroquine disposition. The control group subjects received two tablets of chloroquine sulfate (300-mg base) only. The test group subjects received ranitidine 250 mg hs for four days prior to the administration of chloroquine sulfate and throughout the sample collection period. Blood samples (5 ml) were collected from the time of the chloroquine administration to the seventh day after drug administration. The samples were analyzed for chloroquine content by a combination of thin-layer chromatography and ultraviolet spectrophotometry. The Wilcoxon test at 0.05 significance level was used to compare the disposition parameters between control and test groups. Ranitidine therapy was associated with no significant alterations in chloroquine oral clearance rate, elimination rate constant, and apparent volume of distribution. Unlike cimetidine, ranitidine does not interact pharmacokinetically with chloroquine. Ranitidine, therefore, may be the H2-receptor antagonist of choice for ulcer patients receiving chloroquine.
Assuntos
Cloroquina/farmacocinética , Ranitidina/farmacologia , Adulto , Cloroquina/sangue , Antagonistas dos Receptores H2 da Histamina , Humanos , Masculino , Taxa de Depuração MetabólicaRESUMO
Simultaneous milk and saliva samples were collected over a seven-day period from five lactating subjects after they had ingested two tablets of chloroquine sulfate (300 mg base). The samples were analyzed for chloroquine content by use of a combination of thin-layer chromatography and spectrophotometry. Regression analysis of two variables subject to error was used to determine the milk:saliva levels relationship. The Student's t test for paired data at .05 level of significance was used to evaluate the relationship between chloroquine levels in milk and saliva. The milk:saliva chloroquine concentrations ratio obtained by regression analysis was 0.89 +/- 0.08 (r = .93; P less than .05), and this compared favorably with the value of 0.99 +/- 0.07 obtained from the ratio of the AUC. There were no significant differences in the Tmax, Cmax, AUC, clearance, and elimination half-life values of chloroquine in milk and saliva (P greater than .05). Milk and saliva appear to be part of the central compartment. The amount of chloroquine estimated to be consumed by a nursling over a 24-hour period is about 0.55% of a 300-mg dose consumed by the mother. The data suggest that milk chloroquine levels can be estimated from saliva levels.
Assuntos
Cloroquina/farmacocinética , Leite Humano/metabolismo , Adulto , Cromatografia em Camada Fina , Feminino , Meia-Vida , Humanos , Saliva/metabolismo , Espectrofotometria UltravioletaRESUMO
The use of saliva levels as an alternative to plasma levels in monitoring chloroquine therapy was studied in five healthy volunteers. Subjects took two (250 mg) tablets of chloroquine diphosphate (300 mg chloroquine base) with 200 ml of water. Saliva and blood samples were collected at intervals over 6 days. Plasma was separated from blood samples after centrifugation while saliva samples were centrifuged to remove mucoid sediments. Both the plasma and saliva samples were analysed for chloroquine by a combination of thin-layer chromatography and spectrophotometry. Regression analysis was used to determine the correlation between saliva and plasma chloroquine levels. A significant correlation (r = 0.97, p less than 0.05) was observed between saliva and plasma levels of chloroquine. The saliva: plasma concentrations ratio was found to be 0.53. From the saliva: plasma relationship, the extent of chloroquine binding to plasma proteins was estimated to be 47%. The Cmax and AUC0-6 day values obtained from saliva data was about half those from plasma, while the Tmax obtained from both fluids remained the same. The saliva clearance rate (Cls/F) of chloroquine was about twice the plasma clearance rate (Cl/F). (Cls/F: 0.46 +/- 0.051/day/kg; 0.27 +/- 0.021/day/kg). However, the predicted Cl/F (0.27 +/- 0.031/day/kg) from saliva data was in agreement with Cl/F from plasma data. This was also true of the volume of distribution. The collection of saliva for measuring chloroquine levels provides a painless, non-invasive alternative to plasma sampling, and it is useful in predicting chloroquine pharmacokinetics.
Assuntos
Cloroquina/metabolismo , Saliva/metabolismo , Adulto , Cloroquina/sangue , Cromatografia em Camada Fina , Meia-Vida , Humanos , Cinética , MasculinoRESUMO
The effects of chloroquine and didesethylchloroquine on the rabbit isolated perfused heart and on calcium binding and accumulating ability of the heart mitochondria were investigated. The drugs produced negative chronotropy, negative inotropy and a decrease in coronary flow rate in the isolated perfused myocardium. Both chloroquine and didesethylchloroquine significantly decreased mitochondrial calcium binding and accumulation. These results suggest that the cardiodepressant actions of chloroquine could be due in part to alterations in the calcium accumulating abilities of the mitochondrial membranes, and that didesethylchloroquine, among other metabolites, does contribute significantly to the total observed effect of chloroquine on the cardiovascular system.
Assuntos
Cloroquina/análogos & derivados , Cloroquina/farmacologia , Coração/efeitos dos fármacos , Mitocôndrias Cardíacas/efeitos dos fármacos , Animais , Cálcio/metabolismo , Circulação Coronária/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , Técnicas In Vitro , Luz , Mitocôndrias Cardíacas/metabolismo , Contração Miocárdica/efeitos dos fármacos , Coelhos , Espalhamento de RadiaçãoRESUMO
The interaction of metronidazole with calcium to form a water-soluble complex has been studied by titration with ethylenediaminetetraacetate (EDTA), direct current and pulse polarographic reduction steps of the nitro-group at pH 5 and 7, and by ultraviolet absorption. Stoichiometric calculations, X-ray powder diffraction pattern of the synthesized metronidazole-calcium complex, and molecular ion peak at m/z 381 in the mass spectrum of this product, showed that a 2:1 complex is formed. The interaction of metronidazole with calcium on myocardial contractile performance of guinea-pig electrically-driven isolated left atria in physiological solution was also examined. Metronidazole induced a sustained, concentration-dependent depression of the tension that was reversed by changing the bathing fluid to physiological solution, and/or by adding excess calcium ion. The drug-induced negative inotropic response was antagonized competitively by increasing calcium ion in the bath, whereas noradrenaline antagonized metronidazole-induced negative inotropic responses non-competitively. Addition of the metronidazole-calcium complex to the bath did not affect normal myocardial contractile performance. The results show that metronidazole produces a direct negative inotropic effect on isolated atrial muscles by interfering with Ca2+, and by preventing Ca2+ function in the events leading to contractile activity of atrial muscles.