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1.
Mech Dev ; 106(1-2): 151-4, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11472846

RESUMO

A Drosophila gene encoding a novel zinc-finger protein, Meics, was cloned using a monoclonal antibody. The predicted amino acid sequence contains 12 zinc-finger motifs of the C2H2-type. During spermatogenesis, Meics distributes intranuclearly at pre- and post-meiotic stages whereas it relocates to central-spindle microtubules at both meiotic divisions.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Núcleo Celular/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas de Drosophila , Proteínas de Insetos/metabolismo , Meiose , Microtúbulos/metabolismo , Dedos de Zinco , Sequência de Aminoácidos , Animais , Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/genética , Clonagem Molecular , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Drosophila , Genes de Insetos , Proteínas de Insetos/química , Proteínas de Insetos/genética , Masculino , Dados de Sequência Molecular , Espermatogênese
2.
Bioessays ; 23(3): 242-50, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11223881

RESUMO

The programmed elimination of part of the genome through chromosome loss or chromatin diminution constitutes an exceptional biological process found to be present in several diverse groups of organisms. The occurrence of this phenomenon during early embryogenesis is generally correlated to somatic versus germ-line differentiation. A most outstanding example of chromosome elimination and genomic imprinting is found in sciarid flies, where whole chromosomes of exclusive parental origin are selectively eliminated at different developmental stages. Three types of tissue-specific chromosome elimination events occur in sciarids. During early cleavages, one or two X paternal chromosomes is/are discarded from somatic cells of embryos which then develop as females or males respectively. Thus, the sex of the embryo is determined by the number of eliminated paternal X chromosomes. In germ cells, instead, a single paternal X chromosome is eliminated in embryos of both sexes. In addition, while female meiosis is orthodox, male meiosis is highly unusual as the whole paternal chromosome set is discarded from spermatocytes. As a consequence, only maternally derived chromosomes are included in the functional sperm. This paper reviews current cytological and molecular knowledge on the tissue-specific cell mechanisms evolved to achieve chromosome elimination in sciarids.


Assuntos
Dípteros/embriologia , Dípteros/genética , Embrião não Mamífero/fisiologia , Animais , Ciclo Celular/genética , Deleção Cromossômica , Feminino , Masculino , Modelos Genéticos , Morfogênese , Cromossomo X
3.
Genetics ; 156(4): 1853-65, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11102379

RESUMO

In Drosophila melanogaster and in Sciara ocellaris dosage compensation occurs by hypertranscription of the single male X chromosome. This article reports the cloning and characterization in S. ocellaris of the gene homologous to maleless (mle) of D. melanogaster, which implements dosage compensation. The Sciara mle gene produces a single transcript, encoding a helicase, which is present in both male and female larvae and adults and in testes and ovaries. Both Sciara and Drosophila MLE proteins are highly conserved. The affinity-purified antibody to D. melanogaster MLE recognizes the S. ocellaris MLE protein. In contrast to Drosophila polytene chromosomes, where MLE is preferentially associated with the male X chromosome, in Sciara MLE is found associated with all chromosomes. Anti-MLE staining of Drosophila postblastoderm male embryos revealed a single nuclear dot, whereas Sciara male and female embryos present multiple intranuclear staining spots. This expression pattern in Sciara is also observed before blastoderm stage, when dosage compensation is not yet set up. The affinity-purified antibodies against D. melanogaster MSL1, MSL3, and MOF proteins involved in dosage compensation also revealed no differences in the staining pattern between the X chromosome and the autosomes in both Sciara males and females. These results lead us to propose that different proteins in Drosophila and Sciara would implement dosage compensation.


Assuntos
Proteínas Cromossômicas não Histona , DNA Helicases , Proteínas de Ligação a DNA , Dípteros/genética , Mecanismo Genético de Compensação de Dose , Proteínas de Drosophila , Drosophila melanogaster/genética , Genes de Insetos , Proteínas de Insetos/genética , RNA Helicases/genética , Fatores de Transcrição/genética , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Núcleo Celular/ultraestrutura , Cromossomos/química , Clonagem Molecular , Dípteros/embriologia , Drosophila melanogaster/embriologia , Embrião não Mamífero/metabolismo , Embrião não Mamífero/ultraestrutura , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Insetos/imunologia , Proteínas de Insetos/fisiologia , Masculino , Dados de Sequência Molecular , RNA Helicases/imunologia , RNA Helicases/fisiologia , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Processos de Determinação Sexual , Especificidade da Espécie , Fatores de Transcrição/imunologia , Fatores de Transcrição/fisiologia , Transcrição Gênica , Cromossomo X/genética
5.
J Cell Sci ; 111 ( Pt 6): 723-35, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9472001

RESUMO

We have identified a 227 kDa spindle- and centromere-associated protein in Parascaris, designated PUMA1 (Parascaris univalens mitotic apparatus), using a monoclonal antibody (mAb403) generated against Parascaris embryonic extracts. PUMA1 distribution was studied by immunofluorescence microscopy in mitotic and meiotic Parascaris cells, where centromere organization differs greatly. In mitosis, PUMA1 associates throughout cell division with the centrosomes and kinetochore-microtubules, and it concentrates at the continuous centromere region of the holocentric chromosomes. PUMA1 also localizes to the spindle mid-zone region during anaphase and at the midbody during telophase. In meiosis, PUMA1 associates with the centrosomes and with the discrete centromeric regions lacking kinetochore structures. The analysis of colchicine-treated embryos indicated that the association of PUMA1 with the centromeric region depends on microtubule integrity. mAb403 also recognizes spindle components in Drosophila. A series of overlapping cDNAs encoding the gene were isolated from a Parascaris embryonic expression library. Analysis of the nucleotide sequence identified an open reading frame capable of encoding a protein of 227 kDa. Analysis of the protein sequence indicated that PUMA1 is predicted to be a coiled-coil protein containing a large central alpha-helical domain flanked by nonhelical terminal domains. The structural features and cellular distribution of PUMA1 suggest that it may play a role in the organization of the spindle apparatus and in its interaction with the centromere in Parascaris.


Assuntos
Proteínas de Ciclo Celular , Centrômero/metabolismo , Centrossomo/metabolismo , Proteínas Nucleares/metabolismo , Fuso Acromático/metabolismo , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/metabolismo , Antígenos de Helmintos/química , Antígenos de Helmintos/genética , Ascaridoidea , Sequência de Bases , DNA Complementar/química , DNA Complementar/isolamento & purificação , Drosophila melanogaster , Proteínas de Helminto/genética , Masculino , Meiose/imunologia , Mitose/imunologia , Dados de Sequência Molecular , Proteínas Nucleares/química , Proteínas Nucleares/genética
6.
J Cell Sci ; 110 ( Pt 6): 721-30, 1997 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9099946

RESUMO

Spindle formation and chromosome elimination during male meiosis in Sciara ocellaris (Diptera, Sciaridae) has been studied by immunofluorescence techniques. During meiosis I a monopolar spindle is formed from a single polar complex (centrosome-like structure). This single centrosomal structure persists during meiosis II and is responsible for the non-disjunction of the maternal X chromatids. During meiosis I and II non-spindle microtubules are assembled in the cytoplasmic bud regions of the spermatocytes. The chromosomes undergoing elimination during both meiotic divisions are segregated to the bud region where they associate with bundles of microtubules. The presence and distribution of centrosomal antigens in S. ocellaris meiotic spindles and bud regions has been investigated using different antibodies. gamma-Tubulin and centrin are present in the bud as well as in the single polar complex of first meiotic spindle. The results suggest that spermatocyte bud regions contain microtubule-organizing centres (MTOCs) that nucleate cytoplasmic microtubules that are involved in capturing chromosomes in the bud regions. The distribution of actin and myosin in the spermatocytes during meiosis is also reported.


Assuntos
Deleção Cromossômica , Dípteros/genética , Meiose/genética , Microtúbulos/fisiologia , Actinas/metabolismo , Animais , Centrossomo/metabolismo , Dípteros/citologia , Técnica Indireta de Fluorescência para Anticorpo , Masculino , Miosinas/metabolismo , Espermatócitos/metabolismo , Combinação Trimetoprima e Sulfametoxazol/imunologia , Tubulina (Proteína)/imunologia
7.
J Cell Sci ; 108 ( Pt 6): 2393-404, 1995 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7673358

RESUMO

We have studied the relationship between the occurrence of chromatin diminution and the developmental behavior of somatic blastomeres in early development of the nematode Parascaris univalens. A cytological and immunocytochemical analysis of chromatin diminution was performed in P. univalens embryos exposed to 'vegetalizing' (LiCl) and 'animalizing' (NaSCN) substances during early developmental stages. We have also analyzed chromatin diminution in embryos displaying only symmetrical 'somatic-like' divisions due to a brief cytochalasin B treatment at the pronuclear stage. The results show that LiCl treatment induces chromatin diminution in P0-P4 pregerminal blastomeres while NaSCN treatment prevents it. Pregerminal cells undergoing chromatin diminution in 'vegetalized' embryos behaved like somatic cells with respect to division and cleavage patterns. NaSCN treatment results in undiminuted polynucleated embryos that are not capable of cleavage. In cytochalasin B-pulsed embryos, chromatin diminution occurs in all blastomeres. From our results we conclude that chromatin diminution and somatic cell behavior are not separable and constitute strictly correlated events in Parascaris. Moreover, the results indicate that the segregation of the cytoplasmic factors involved in chromatin diminution in early Parascaris development are microfilament-mediated. Here, we also report the formation of a latter pregerminal cell precursor (P5) not susceptible to LiCl-induced chromatin diminution.


Assuntos
Cromatina/fisiologia , Nematoides/fisiologia , Animais , Diferenciação Celular , Núcleo Celular/fisiologia , Cromatina/ultraestrutura , Embrião não Mamífero/fisiologia , Nematoides/embriologia
8.
J Cell Biol ; 118(1): 23-32, 1992 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-1618905

RESUMO

In Parascaris the mitotic chromosomes of gonial germline cells are holocentric and possess a continuous kinetochore along their entire length. By contrast, in meiotic cells, the centromeric activity is restricted to the heterochromatic tips where direct insertion of spindle microtubules into chromatin without any kinetochore plate is seen. In the presomatic cells of early embryos, which undergo heterochromatin elimination, only euchromatin shows kinetic activity. After developing a technique to separate the very resistant egg shell from the embryos, we studied the cell divisions during early embryogenesis by immunochemical and EM approaches. The results reported here show that in presomatic cells microtubules bind only the euchromatin where a continuous kinetochore plate is present. We also report observations suggesting that the binding of the long kinetochores to the mitotic spindle initiates to a limited number of sites and extends along the entire length, during chromosome condensation. The existence of different centromere stages in different cell types, rends Parascaris chromosomes a very good model to study centromere organization.


Assuntos
Centrômero/metabolismo , Cromatina/metabolismo , Microtúbulos/metabolismo , Mitose/fisiologia , Nematoides/embriologia , Fuso Acromático/metabolismo , Animais , Centrômero/ultraestrutura , Cromossomos/ultraestrutura , Eucromatina , Feminino , Imunofluorescência , Células Germinativas/metabolismo , Heterocromatina/metabolismo , Tubulina (Proteína)/isolamento & purificação
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