High-fat diet effects on contractile performance of isolated mouse soleus and extensor digitorum longus when supplemented with high dose vitamin D.

Evidence suggests vitamin D3 (VD) supplementation can reduce accumulation of adipose tissue and inflammation and promote myogenesis in obese individuals, and thus could mitigate obesity-induced reductions in skeletal muscle (SkM) contractility. However, this is yet to be directly investigated. This study, using the work-loop technique, examined effects of VD (cholecalciferol) supplementation on isolated SkM contractility. Female mice (n = 37) consumed standard low-fat diet (SLD) or high-fat diet (HFD), with or without VD (20,000 IU/kg-1 ) for 12 weeks. Soleus and EDL (n = 8-10 per muscle per group) were isolated and absolute and normalized (to muscle size and body mass) isometric force and power output (PO) were measured, and fatigue resistance determined. Absolute and normalized isometric force and PO of soleus were unaffected by diet (P > 0.087). However, PO normalized to body mass was reduced in HFD groups (P < 0.001). Isometric force of extensor digitorum longus (EDL) was unaffected by diet (P > 0.588). HFD reduced EDL isometric stress (P = 0.048) and absolute and normalized PO (P < 0.031), but there was no effect of VD (P > 0.493). Cumulative work during fatiguing contractions was lower in HFD groups (P < 0.043), but rate of fatigue was unaffected (P > 0.060). This study uniquely demonstrated that high-dose VD had limited effects on SkM contractility and did not offset demonstrated adverse effects of HFD. However, small and moderate effect sizes suggest improvement in EDL muscle performance and animal morphology in HFD VD groups. Given effect sizes observed, coupled with proposed inverted U-shaped dose-effect curve, future investigations are needed to determine dose/duration specific responses to VD, which may culminate in improved function of HFD SkM.

Effect of stimulation frequency on force, power and fatigue of isolated mouse extensor digitorum longus muscle.

This study examined the effect of stimulation frequency (140, 200, 230 and 260 Hz) on isometric force, work loop (WL) power and the fatigue resistance of extensor digitorum longus (EDL) muscle (n=32), isolated from 8- to 10-week-old CD-1 female mice. Stimulation frequency had significant effects on isometric properties of isolated mouse EDL, whereby increasing stimulation frequency evoked increased isometric force, quicker activation and prolonged relaxation (P<0.047) up to 230 Hz and above; thereafter, force and activation did not differ (P>0.137). Increasing stimulation frequency increased maximal WL power output (P<0.001; 140 Hz, 71.3±3.5; 200 Hz, 105.4±4.1; 230 Hz, 115.5±4.1; 260 Hz, 121.1±4.1 W kg-1), but resulted in significantly quicker rates of fatigue during consecutive WLs (P<0.004). WL shapes indicate impaired muscle relaxation at the end of shortening and subsequent increased negative work appeared to contribute to fatigue at 230 and 260 Hz, but not at lower stimulation frequencies. Cumulative work was unaffected by stimulation frequency, except at the start of the fatigue protocol, where 230 and 260 Hz produced more work than 140 Hz (P<0.039). We demonstrate that stimulation frequency affects force, power and fatigue, but these effects are not uniform between different assessments of contractile performance. Therefore, future work examining the contractile properties of isolated skeletal muscle should consider increasing the stimulation frequency beyond that needed for maximal force when examining maximal power but should utilise a sub-maximal stimulation frequency for fatigue assessments to avoid a high degree of negative work atypical of in vivo function.

The effect of fatigue on first stance phase kinetics during acceleration sprint running in professional football players.

Nineteen professional football players (Age: 26±5 years; Height: 1.84±0.08 m; Mass: 83.4±8.9 kg) completed three x 30 m maximal acceleration sprints from a standing start before completing the Yo-Yo intermittent recovery test level 1. Three x 30 m maximal acceleration sprints were then repeated post-fatigue. Light gates recorded sprint times from 0-5 m, 0-10 m, 0-15 m and 0-30 m. Force platforms collected ground reaction force of the first stance phase of the sprint run. Differences between pre- and post-fatigue were observed in the sprint times over 0-15 m (P = 0.015; CI [0.007, 0.110]) and 0-30 m (P = 0.004; CI [0.056, 0.234]). Peak medial-lateral ground reaction force was lower (P = 0.045; CI [-0.146, -0.005]) post- than pre-fatigue. The ratio of force were significantly different between pre- and post-fatigue for the medial-lateral and anterior-posterior comparison (P = 0.017; CI [-0.063, -0.010]), and the medial-lateral and vertical comparison (P = 0.012; CI [-0.036, -0.007]). Football players altered their sprint mechanics to reduce medial-lateral loading and orient the force in an increased anteroposterior and vertical direction in order to maintain 0-10 m sprint performance. Practitioners should observe medial-lateral force contributions and improve sprint technical efficacy.

Angle-Specific Isokinetic Metrics Highlight Strength Training Needs of Elite Youth Soccer Players.

Eustace, SJ, Page, RM, and Greig, M. angle specific isokinetic metrics highlight strength training needs of elite youth soccer players. J Strength Cond Res 34(11): 3258-3265, 2020-The purpose of this study was to assess traditional and angle-specific isokinetic strength of eccentric knee flexors (eccKFs) and concentric knee extensors (conKEs) between senior professional and youth soccer players. Thirty-four male soccer players (17 senior and 17 youth) were recruited for bilateral assessments at 180, 270, and 60°·s. Peak torque (PT), dynamic control ratio (DCR), angle of peak torque (APT), functional range (FR), angle-specific torque (AST), and angle-specific DCR (DCRAST) were compared. The eccentric knee flexor (eccKF) and conKE PT (p = 0.782) and DCR (p = 0.508) were not different between groups across all angular velocities. Significant differences were identified for eccKF APT (p = 0.018) and FR (p = 0.006), DCRAST at 270°·s (p = 0.031), and in AST data recorded across angular velocities for eccKF and conKE (p = 0.003). Traditional strength measures were not sensitive to playing age, with implications for misinterpretation in training prescription. By contrast, AST data did differentiate between ages. Strength deficits that highlight the muscle contraction type, angular velocity, and joint angle can be manipulated within an individualized training intervention. Given the relevance to injury etiology, this study highlights potential implications for improved assessment strategies to inform training prescription for performance and injury prevention. Given the high number of injuries in adolescent soccer players, and in line with previous recommendations, practitioners should consider using more informed and specific strength and conditioning practices at younger ages.

Image quality in low-dose multidetector computed tomography: a pilot study to assess feasibility and dose optimization in whole-body bone imaging.

OBJECTIVE: To study the impact of dose parameters on image quality at whole-body low-dose multidetector computed tomography (CT) in an attempt to derive parameters that allow diagnostic quality images of the skeletal system without incurring significant radiation dose in patients referred for investigation of plasma cell dyscrasias. METHODS: By using a single cadaver, 14 different whole-body low-dose CT protocols were individually assessed by 2 radiologists, blinded to acquisition parameters (kVp and mAs, reconstruction algorithm, dose reduction software). Combinations of kVps that range from 80-140 kVp, and tube current time product from 14-125 mAs were individually scored by using a Likert scale from 1-5 in 4 separate anatomical areas (skull base, thoracic spine, pelvis, and distal femora). Correlation between readers scores and effective doses were obtained by using correlation coefficient statistical analysis, statistical significance was considered P < .01. Interobserver agreement was assessed by using a Bland and Altman plot. Interobserver agreement in each of the 4 anatomical areas was assessed by using kappa statistics. A single set of parameters was then selected for use in future clinical trials in a cohort of patients referred for investigation of monoclonal gammopathy, including multiple myeloma. RESULTS: Several sets of exposure parameters allowed low-dose whole-body CT to be performed with effective doses similar to skeletal survey while preserving diagnostic image quality. Individual reader's and average combined scores showed a strong inverse correlation with effective dose (reader 1, r = -0.78, P = .0001; reader 2, r = -0.75, P = .0003); average combined scores r = -0.81, P < .0001). Bland and Altman plot of overall scores shows reasonable interobserver agreement, with a mean difference of 1.055. CONCLUSION: Whole-body low-dose CT can be used to obtain adequate CT image quality to assess normal osseous detail while delivering effective doses similar to those associated with conventional radiographic skeletal survey.