Solvent-free, Noncontact Electrostatic Sampling for Rapid Analysis with Mass Spectrometry: Application to Drugs and Explosives.

A hand-held Van de Graaf generator is used to apply a high voltage, negligible current electrostatic potential to a wire mesh positioned in close proximity to a particle-laden surface in order to collect those particles for analysis. The electrostatic field effects transfer particles to the mesh without a requirement for mechanical contact between mesh and surface. Analysis of chemicals present in the sampled particles is completed by thermal desorption electrospray ionization. The utility of the method for noncontact sampling is demonstrated using solid drug powder samples, and inorganic explosives dispersed either on solid surfaces or in sand/soil in order to simulate common interfering matrices that might be encountered in the forensic environment. A metal mesh sampling substrate is utilized instead of traditional polymer-based swabs in order to permit thermal desorption at higher temperatures. The method leaves no visible trace of sampling leaving details such as a fingerprint image unperturbed, as demonstrated using fluorescence photography. Direct sampling of trace particles from hard surfaces and skin documents flexibility in the choice of sampling substrates, desorption temperatures, and sampling times. The potential of the device for use in forensic analyses is detailed.

Transmission mode desorption electrospray ionization (TM-DESI) for simultaneous analysis of potential inorganic and organic components of radiological dispersion devices (RDDs).

Transmission mode desorption electrospray ionization (TM-DESI) coupled to an ion trap mass spectrometer capable of source collision-induced dissociation (CID) was used to completely analyze radiological dispersion device components. Source CID significantly enhanced the signal for metal ions by reducing adducts while eliminating chemical noise from background molecules through extensive fragmentation. Source CID spectra yielded reasonably accurate isotopic ratios for the metals studied. By switching the source CID on and off between scans, all major constituents in mixtures of simulated radionuclides and explosives were simultaneously observed. These results indicate that TM-DESI/ion trap technology could be a powerful on-site tool for nuclear forensics.

Protein arrays on patterned porous gold substrates interrogated with mass spectrometry: detection of peptides in plasma.

Methyl- and carboxy-terminated self-assembled monolayers (SAMs) were custom-patterned on porous gold substrates with equipment commonly used to print protein arrays, without complex surface chemistry protocols. Proteins were covalently immobilized on hydrophilic carboxy-terminated SAM spots, while the remainder of the surface was superhydrophobic due to the roughened gold surface and the methyl-terminated SAM. The resistance of these patterns to biofouling and the effective containment of MALDI matrix solution within the hydrophilic spot made these surfaces amenable to analyzing protein-peptide binding with mass spectrometry. A model system of the affinity peptides HA, cmyc, and V5 and their corresponding antibodies was used to demonstrate the utility of the patterned porous gold. Mass spectrometry (MS) and tandem mass spectrometry (MS/MS) matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectra and images obtained reflected the effective capture of the affinity peptides directly from spiked bovine plasma.

Changes in adsorbed fibrinogen upon conversion to fibrin.

The conversion of adsorbed fibrinogen to fibrin in the presence of the enzyme thrombin was studied using surface plasmon resonance (SPR), a quartz crystal microbalance (QCM), sum frequency generation (SFG), atomic force microscopy (AFM), and an elutability assay. Exposure of adsorbed fibrinogen to thrombin resulted in a mass loss at the surface consistent with fibrinopeptide release and conversion to fibrin. Changes in hydration upon conversion of adsorbed fibrinogen to fibrin were determined from comparisons of acoustic (QCM) and optical (SPR) mass adsorption data. Conversion to fibrin also resulted in the adsorbed layer becoming more strongly bound to the surface and more compact. The elutability of adsorbed fibrinogen by Triton X-100, studied with SPR, decreased from 90 +/- 5 to 6 +/- 2% after conversion to fibrin. The height of the adsorbed monolayer, as determined by AFM, decreased from 5.5 +/- 2.2 to 1.7 +/- 0.8 nm. We conclude that thrombin-catalyzed fibrinopeptide release triggers significant changes in fibrinogen conformation beyond peptide cleavage.

Interactions of thrombin with fibrinogen adsorbed on methyl-, hydroxyl-, amine-, and carboxyl-terminated self-assembled monolayers.

We have examined the initial phase of fibrin formation, thrombin-catalyzed fibrinopeptide cleavage, from adsorbed fibrinogen using surface plasmon resonance and liquid chromatography-mass spectrometry. Fibrinogen adsorption impaired thrombin-fibrinogen interactions compared to the interactions of thrombin with fibrinogen in solution. The properties of the underlying substrate significantly affected the extent and kinetics of fibrinopeptide cleavage, and the conversion of adsorbed fibrinogen to fibrin. Fibrinogen adsorbed on negatively charged surfaces (carboxyl-terminated self-assembled monolayers) released a smaller amount of fibrinopeptides, at a reduced rate relative to those of hydrophobic, hydrophilic, and positively charged surfaces (methyl-, hydroxyl-, and amine-terminated self-assembled monolayers, respectively). Additionally, the conversion of adsorbed fibrinogen to fibrin was comparatively inefficient at the negatively charged surface. These data correlated well with trends previously reported for fibrin proliferation as a function of surface properties. We conclude that thrombin interactions with adsorbed fibrinogen determine the extent of subsequent fibrin proliferation on surfaces.

Fibrin proliferation at model surfaces: influence of surface properties.

Fibrin proliferation from both human fibrinogen solutions and platelet-poor plasma was studied quantitatively as a function of substrate surface properties. A quartz crystal microbalance was used to monitor both protein adsorption and fibrin proliferation in real time at hydrophobic, hydrophilic, positively charged, and negatively charged surfaces. Scanning electron microscopy was used to characterize the morphology of the polymerized fibrin layers. The observed changes in mass indicate that fibrinogen adsorption occurs rapidly and mediates subsequent fibrin proliferation. Notably, substrate surface properties significantly affect the ability of adsorbed fibrinogen to promote fibrin proliferation.