Polymethylmethacrylate-hydroxyapatite antibacterial and antifungal activity against oral bacteria: An in vitro study.

Objective: Reconstruction of alveolar bone defects resulting from aging, trauma, ablative surgery or pathology, remains a significant clinical challenge. The objective of this study was to investigate the antibacterial and antifungal activities of mixed polymethylmethacrylate-hydroxyapatite (PMMA-HA) against oral microorganisms. Our findings could provide valuable insights into the prospective application of PMMA-HA as a synthetic bone graft material to manage alveolar bone defects via tissue engineering. Methods: HA powder was obtained from the Center for Ceramics in Indonesia and PMMA granules were obtained from HiMedia Laboratories; these were prepared in 20:80, 30:70, and 40:60 ratios. The antibacterial diffusion method was then performed against Staphylococcusaureus, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum, while the antifungal diffusion method was used to test against Candida albicans. Standardized protocols were used for microbial culturing and inhibition zones were measured with digital calipers. Statistical analyses included one-way ANOVA and Kruskal-Wallis tests, supplemented by post-hoc Tukey HSD tests. Results: A PMMA-HA scaffold with a 20:80 ratio demonstrated the highest antibacterial activity against S. aureus, A. actinomycetemcomitans, P. gingivalis, and F. nucleatum. This was followed by the 30:70 and 40:60 ratios in terms of antibacterial activity. Statistical significance was achieved with p < 0.05 in comparison to controls. However, none of the PMMA-HA ratios showed antifungal activity against C. albicans. Conclusion: PMMA-HA scaffolds have significant activity against bacteria, but not against fungi.

The Effect of Centella asiatica Methanolic Extract on Expression of IL-1ß Proinflammatory Cytokines in Severe Early Childhood Caries.

OBJECTIVE: This article analyzes the role of C. asiatica extract in reducing the proinflammatory cytokine interleukin (IL)-1ß produced by salivary neutrophils. RESULTS: The administration of methanolic extract C. asiatica decreased the expression of the proinflammatory cytokine IL-1ß on the surface of salivary neutrophils on S-ECC; The administration of C. asiatica methanol extract resulted in a decrease in the expression of the proinflammatory cytokine IL-1ß on the surface of salivary neutrophils in S-ECC. CONCLUSION: C. asiatica extract has the effect of reducing the proinflammatory cytokine IL-1ß produced by salivary neutrophils on S-ECC via inhibiting nuclear factor kappa B and mitogen-activated protein kinase signaling pathway activation and suggest that C. asiatica is a possible candidate for treating S-ECC.