RESUMO
Accumulating evidence from experimental and clinical studies suggest that retinal ganglion cells at least in the earlier stages of glaucoma have the capacity to recover function following periods of functional loss. The capacity for recovery may be negatively impacted by advancing age but can be boosted by interventions such as diet restriction and exercise.
Assuntos
Glaucoma/fisiopatologia , Recuperação de Função Fisiológica/fisiologia , Células Ganglionares da Retina/fisiologia , Envelhecimento/fisiologia , Animais , Dieta , Modelos Animais de Doenças , Exercício Físico/fisiologia , HumanosRESUMO
It has been established that beyond middle age, mice are slower to recover inner retinal function following an acute intraocular pressure (IOP) injury. While 3 month old animals exhibit near-complete recovery 1 week following injury, 12 and 18 month old animals demonstrate prolonged inner retinal dysfunction. In this study we aim to determine whether age-related differences in functional recovery of the inner retina are due to differences in retinal ganglion cell (RGC) axonal transport. C57BL/6J mice at 3 (n = 8) and 18 months (n = 8) of age were used. At day 0, right eyes were cannulated and the IOP was maintained at 50 mmHg for 30 min. At day 5, mice received bilateral intravitreal injections of choleratoxin subunit B (CTB) conjugated to Alexafluor 488. At day 7, mice were euthanized and tissue was collected. Axonal transport of CTB was quantified in retinas and superior colliculi (SC) using fluorescent microscopy. In response to IOP elevation, the overall degree of axonal transport was comparable between young and old mice. Furthermore, no differences in axonal transport were detected between control eyes and injured in mice at any age. In conclusion, impaired recovery of inner retinal function 1 week following acute IOP injury in old mice is not associated with changes in active axonal transport in RGCs at this time.
Assuntos
Envelhecimento/fisiologia , Transporte Axonal/fisiologia , Traumatismos do Nervo Óptico/fisiopatologia , Recuperação de Função Fisiológica/fisiologia , Doenças Retinianas/fisiopatologia , Células Ganglionares da Retina/fisiologia , Animais , Modelos Animais de Doenças , Eletrorretinografia , Pressão Intraocular/fisiologia , Camundongos , Camundongos Endogâmicos C57BLRESUMO
We describe the novel use of semitendinosus as a tendon graft for 2-stage flexor digitorum profundus (FDP) reconstruction. To our knowledge, this is the first reported use of a hamstring tendon graft in this setting. The FDP of two digits were reconstructed in a 30 year-old male who presented 18 years after the original injury. The semitendinosus was chosen as a graft as the traditional grafts were deemed inappropriate. The result of the operation is convincing, and we suggest the semitendinosus tendon to be considered an option for FDP reconstruction.
