Human occupation continuity in southern Italy towards the end of the Middle Palaeolithic: a palaeoenvironmental perspective from Apulia.

After the last interglacial [Marine Isotope Stage (MIS) 5e] Europe was affected by several harsh climatic oscillations. In this context southern Italy acted, like the rest of peninsular Mediterranean Europe, as a 'glacial refugium', allowing the survival of various species, and was involved in the spread of 'cold taxa' (e.g. woolly mammoth and woolly rhino) only during the coldest phases (MIS 4 and MIS 2). Both late Mousterian and early Upper Palaeolithic sites testify to a human occupation continuity in southern Italy and especially in Apulia in this time span. Here we present a focus on three key Apulian Palaeolithic sequences (Grotta di Santa Croce, Riparo L'Oscurusciuto and Grotta del Cavallo - layers F-E) jointly spanning from the late MIS 4 to the demise of Neanderthals around 43 ka. Novel chronological, sedimentological and zooarchaeological data are discussed for the first time in the light of the palaeoenvironmental information provided by recent analyses carried out on a speleothem from Pozzo Cucù cave (Bari) and the results of the magnetic susceptibility analysis from Riparo L'Oscurusciuto. This integrated reading allows a better understanding of the role played by the Apulian region as both a refugium for late Neaderthals and a suitable habitat for the early settling of modern humans.

Raman and ATR-FTIR analyses of medieval wall paintings from al-Qarawiyyin in Fez (Morocco).

A multi-analytical study has been undertaken to characterise wall paintings from the al-Qarawiyyin university in Fez, dating back to the 9th century and still in activity, with the aim of identifying the pigments used and going back to the painting techniques adopted by the Moroccan craftsmen of the time. The investigation crossed Raman and ATR-FTIR structural analyses along with XRF elemental ones, as well as colorimetric measurements. The colours and shades of six representative fragments collected during a recent excavation have been examined. Hence, red ochre (mainly hematite) was used to obtain brown-red colour, calcite for white, bistre for carbon-based black, while blue shades were achieved using azurite. Mixtures of pigments were created to widen the colour palette: cinnabar, minium and hematite were identified in orange hues while lapis lazuli and azurite were observed in grey-blue areas. The exploration of mortar layers revealed gypsum, gypsum/calcite and calcite type plasters. Furthermore, the observation of the morphological aspect of the interface between the plaster and the painting layer seems indicating that the lime-secco-painting skill had been the adopted painting technique.

Late Acheulian Jaljulia - Early human occupations in the paleo-landscape of the central coastal plain of Israel.

The Lower Paleolithic Late Acheulian in the Levant marks a fascinating chapter in human cultural and biological evolution. Nevertheless, many aspects of the Late Acheulian are still undeciphered, hindered by the complex nature of each site on the one hand, a scarcity of wide, multidisciplinary studies on the other, and by difficulties in obtaining absolute chronology for this timeframe. Therefore, subjects such as human subsistence strategies and modes of adaptation, regional diversity, and the possible existence and nature of interactions between hominin groups are largely understudied. The discovery and study of Jaljulia, a large-scale Late Acheulian site at the central Coastal Plain, Israel, add valuable insights to the research of this chapter in human history. Considered to represent recurrent occupations at a favored, water and flint-rich setting, the site has provided extensive lithic assemblages obtained from several localities. Absolute chronology places the human activity on-site at roughly 500-300 ky (and possibly even later), which is suggested to be divided into several main occupation phases. Geomorphological and sedimentological analyses show a change in environmental conditions, from aeolian sand deposition and overlying Hamra soil during the Middle Pleistocene to high energy fluvial regime which transported large gravels in a north-south paleo-channel. Wetland environments, correlating to the human activity on site, developed later due to higher sea levels and a coastline shifts to the eastward, which caused a blockage of the Yarkon stream corridor to the sea by marine sand. In this paper we present results of the study of the site, including geomorphological formation and post-depositional processes, absolute chronology, lithic and faunal analyses. The site's extensive lithic assemblages are currently under study and future investigations are expected to shed more light on the technological nature of Late Acheulian Jaljulia.

An improved chronology for the Middle Stone Age at El Mnasra cave, Morocco.

North African coastal Middle Stone Age (MSA) sites are key to study the development and expansion of early H. sapiens. El Mnasra cave on the Atlantic coast of Morocco (Témara region) is a crucial site associated with MSA archaeological materials considered advanced cognitive hallmarks of behavioural innovation, such as numerous Nassariidae perforated shells, hematite pigments, bones industry and coastal resources exploitation. We provide new trapped-charges dates (OSL and combined US-ESR ages). Our Bayesian modelling strengthens the new lithostratigraphic interpretation of the cave stratigraphic units (US) and we propose an updated chronostratigraphic model for the Middle Stone Age archaeo-sequence of El Mnasra Cave. We confirm a human presence between 124-104 ka, earlier than what the previous OSL and US-ESR data showed. Our time range intervals allowed us to also extend the age of the MSA occupations considerably to the MIS 4/3 (~62-30 ka), marked by the disappearance of the Nassariidae perforated shells. Outstandingly, our model pushed back the age of the largest record of Nassariidae perforated shells and placed the age of their use by the Aterian groups at El Mnasra from the MIS 5d-5b (~115-94 ka).

Reappraisal of the chronology of Orgnac 3 Lower-to-Middle Paleolithic site (Ardèche, France), a regional key sequence for the Middle Pleistocene of southern France.

Previous studies have suggested that the Lower-to-Middle Paleolithic transition was associated with the earliest Neanderthals, but recent research has established that the oldest Neanderthal fossils and the first signs of their technologies and behavior appear from MIS 11 or possibly earlier. To understand these changes, re-evaluation of the evidence is necessary to determine if this transition corresponds to a progressive evolution rather than abrupt change. Orgnac 3 is a key and appropriate site to study this research context. Located in southern France, it yields a long stratigraphic sequence testifying the evolution of technical and subsistence behaviors of pre-Neanderthal human groups during a Middle Pleistocene interglacial-glacial cycle. In this article, a new chronological framework is provided for the sequence based on results of dating methods applied to various types of geological materials. Speleothems and volcanic minerals, dated in previous studies by U-series and 40Ar/39Ar, respectively, show periods of calcitic crystallization and regional volcanic activity. Other materials, such as heated flints and herbivore teeth, are directly related to evidence of anthropogenic activities and are analyzed in the present work by trapped-charge dating methods such as thermoluminescence and electron spin resonance combined with uranium series (ESR/U-series). The new thermoluminescence and ESR/U-series dates confirm the attribution of the Orgnac 3 stratigraphic sequence to the MIS 10-MIS 8 period and are discussed in relation to paleoenvironmental data derived from bioarchaeological studies. The paleoanthropological levels, including the emergence of Levallois technology, are dated to ca. 275 ka (early MIS 8) and appear coeval to a wet and temperate period recorded locally, the Amargiers interstadial, defined in the regional palynological records. The implications of this reassessed chronology for the archaeological assemblages are discussed in the wider context of behavioral innovations from MIS 11 onward and their establishment in subsequent periods.

Earliest African evidence of carcass processing and consumption in cave at 700 ka, Casablanca, Morocco.

To date, in Africa, evidence for animal processing and consumption in caves routinely used as living spaces is only documented in the late Middle Pleistocene of the North and South of the continent and postdates the Middle Pleistocene in East Africa. Here we report the earliest evidence in a North-African cave (Grotte des Rhinocéros at Casablanca, Morocco) of cut, percussion and human gnawing marks on faunal remains directly associated with lithic knapping activities in the same space and in a well-documented stratified context. Ages for this Acheulean site are provided by the dating of herbivorous teeth to 690-720 ka and 520-550 ka (lower and upper sets) by combined Electron Spin Resonance (ESR) and U-series techniques. Traces of butchery on gazelle, alcelaphin, and zebra bones demonstrate that hominins had primary access to herbivore carcasses. Hominins brought and consumed meat in the cave, as documented by herbivore bones bearing human tooth marks concentrated in a circumscribed area of the excavation. In Africa, this site provides the earliest evidence for in situ carcass processing and meat-eating in cave, directly associated with lithic production and demonstrates the recurrent use by early Middle Pleistocene hominins of a North African cave site 400 000 years before that by Homo sapiens at Jebel Irhoud (Morocco).

Fire and brief human occupations in Iberia during MIS 4: Evidence from Abric del Pastor (Alcoy, Spain).

There is a relatively low amount of Middle Paleolithic sites in Europe dating to MIS 4. Of the few that exist, several of them lack evidence for anthropogenic fire, raising the question of how this period of global cooling may have affected the Neanderthal population. The Iberian Peninsula is a key area to explore this issue, as it has been considered as a glacial refugium during critical periods of the Neanderthal timeline and might therefore yield archaeological contexts in which we can explore possible changes in the behaviour and settlement patterns of Neanderthal groups during MIS 4. Here we report recent data from Abric del Pastor, a small rock shelter in Alcoy (Alicante, Spain) with a stratified deposit containing Middle Palaeolithic remains. We present absolute dates that frame the sequence within MIS 4 and multi-proxy geoarchaeological evidence of in situ anthropogenic fire, including microscopic evidence of in situ combustion residues and thermally altered sediment. We also present archaeostratigraphic evidence of recurrent, functionally diverse, brief human occupation of the rock shelter. Our results suggest that Neanderthals occupied the Central Mediterranean coast of the Iberian Peninsula during MIS 4, that these Neanderthals were not undergoing climatic stress and they were habitual fire users.

New electron spin resonance (ESR) ages from Geißenklösterle Cave: A chronological study of the Middle and early Upper Paleolithic layers.

Geißenklösterle Cave (Germany) is one of the most important Paleolithic sites in Europe, as it is characterized by human occupation during the Middle and early Upper Paleolithic. Aurignacian layers prior to 37-38 ka cal BP feature both musical and figurative art objects that are linked to the early arrival in Europe of Homo sapiens. Middle Paleolithic layers yielded lithic artifacts attributed to Homo neanderthalensis. Since human occupation at the site is attributed to both Neanderthals and modern humans, chronology is essential to clarify the issues of Neanderthal disappearance, modern human expansion in Europe, and the origin of the Aurignacian in Western Europe. Electron spin resonance (ESR) dating was performed on fossil tooth enamel collected from the Middle Paleolithic layers, which are beyond the radiocarbon dating range, and from the nearly sterile 'transitional' geological horizon (GH) 17 and the lower Aurignacian deposits, to cross-check ESR ages with previous radiocarbon, thermoluminescence and ESR age results. The Middle Paleolithic layers were dated between 94 ± 10 ka (GH 21) and 55 ± 6 ka (GH 18) by ESR on tooth enamel. Mean ages for GH 17, at 46 ± 3 ka, and for the lower Aurignacian layers, at 37 ± 3 ka, are in agreement with previous dating results, thus supporting the reliability of ESR chronology for the base of the sequence where dating comparisons are not possible. These results suggest that Neanderthals occupied the site from Marine Isotope Stage (MIS) 5 to the second half of MIS 3 and confirm the antiquity of early Aurignacian deposits. The presence of an almost sterile layer that separates Middle and Upper Paleolithic occupations could be related to the abandonment of the site by Neanderthals, possibly during Heinrich Stadial 5 (ca. 49-47 ka), thus before the arrival of H. sapiens in the area around 42 ka cal BP. These dates for the Middle Paleolithic of the Swabian Jura represent an important contribution to the prehistory of the region, where nearly all of the excavations were conducted decades ago and prior to the development of reliable radiometric dating beyond the range of radiocarbon.

Timing of the Brunhes-Matuyama transition constrained by U-series disequilibrium.

U-series disequilibrium measurements carried out on thermogenic travertine samples from a 12.6 m-long core and a 10 m-thick section from southeastern Morocco yielded finite ages ranging from 500 ka to the present-day, as well as two clusters determined to be older than 500 ka. The calculation of initial 234U/238U activity ratios in all samples younger than 500 ka shows high, reasonably constant values, with an average of 5.172 ± 0.520 (one standard deviation). Assuming that this value prevailed for periods older than 500 ka, we derived ages of up to approximately 1.2 Ma using the initial 234U excess decay. Our results indicate that the two older clusters have ages of 776 ± 14 ka for samples from between 8 and 10.1 m and 1173 ± 22 ka for deeper samples respectively. The palaeomagnetic record of the core shows normal polarity inclinations from the surface to around 9 m followed by reverse polarity inclination and antipodal declinations. The inversion is attributed to the Brunhes-Matuyama transition. 234U excess ages for the interval corresponding to the part of the core where the polarity inversion occurred are in the range of 735 ± 51 to 794 ± 54 ka, with an arithmetic mean value of 776 ± 14 ka for the B-M transition. This age is in good agreement with that determined previously using other dating methods.

U-Th dated speleothem recorded geomagnetic excursions in the Lower Brunhes.

The study of geomagnetic excursions is key for understanding the behavior of the magnetic field of the Earth. In this paper, we present the geomagnetic record in a 2.29-m-long continuous core sampled in a flowstone in Liguria (Italy) and dated to the Lower Brunhes. The cored flowstone developed from Marine Isotopic Stage (MIS) 13 to MIS 7, according to 21 U-series dates. The mean growth rate is closely related to glacial and interglacial isotopic stages. Magnetic remanence was measured using u-channel and deconvolved. Four geomagnetic excursions were recorded at the same location, in a single flowstone, during interglacial MIS 11 and 13; Basura 1, 2, 3 and 4, at depths of 213 cm, 181, 160 and 92 cm, respectively. Due to the uncertainties of U-Th dating, the timing of the three events, namely Basura 1, 2 and 3 overlaps. The Basura 4 is well-dated to 417 + -7/8 ka and is clearly distinguishable from the others. It should therefore be considered as a possible excursion.

A Human Deciduous Tooth and New 40Ar/39Ar Dating Results from the Middle Pleistocene Archaeological Site of Isernia La Pineta, Southern Italy.

Isernia La Pineta (south-central Italy, Molise) is one of the most important archaeological localities of the Middle Pleistocene in Western Europe. It is an extensive open-air site with abundant lithic industry and faunal remains distributed across four stratified archaeosurfaces that have been found in two sectors of the excavation (3c, 3a, 3s10 in sect. I; 3a in sect. II). The prehistoric attendance was close to a wet environment, with a series of small waterfalls and lakes associated to calcareous tufa deposits. An isolated human deciduous incisor (labelled IS42) was discovered in 2014 within the archaeological level 3 coll (overlying layer 3a) that, according to new 40Ar/39Ar measurements, is dated to about 583-561 ka, i.e. to the end of marine isotope stage (MIS) 15. Thus, the tooth is currently the oldest human fossil specimen in Italy; it is an important addition to the scanty European fossil record of the Middle Pleistocene, being associated with a lithic assemblage of local raw materials (flint and limestone) characterized by the absence of handaxes and reduction strategies primarily aimed at the production of small/medium-sized flakes. The faunal assemblage is dominated by ungulates often bearing cut marks. Combining chronology with the archaeological evidence, Isernia La Pineta exhibits a delay in the appearance of handaxes with respect to other European Palaeolithic sites of the Middle Pleistocene. Interestingly, this observation matches the persistence of archaic morphological features shown by the human calvarium from the Middle Pleistocene site of Ceprano, not far from Isernia (south-central Italy, Latium). In this perspective, our analysis is aimed to evaluate morphological features occurring in IS42.

Early evidence of Acheulean settlement in northwestern Europe--la Noira site, a 700,000 year-old occupation in the center of France.

The human settlement of Europe during Pleistocene times was sporadic and several stages have been recognized, both from paleaoanthropological and archaeological records. If the first phase of hominin occupation (as early as 1.4 Ma) seems mainly restricted to the southern part of the continent, the second phase, characterized by specific lithic tools (handaxes), is linked to Acheulean settlements and to the emergence of Homo heidelbergensis, the ancestor of Neanderthals. This phase reached northwestern Europe and is documented in numerous sites in Germany, Great Britain and northern France, generally after 600 ka. At la Noira (Brinay, Central France), the Middle Pleistocene alluvial formation of the Cher River covers an archaeological level associated with a slope deposit (diamicton). The lithic assemblage from this level includes Large Cutting Tools (LCTs), flakes and cores, associated with numerous millstone slabs. The lithic series is classified as Acheulean on the basis of both technological and typological analyses. Cryoturbation features indicate that the slope deposits and associated archaeological level were strongly frozen and disturbed after hominin occupation and before fluvial deposition. Eight sediment samples were dated by the electron spin resonance (ESR) method and the weighted average age obtained for the fluvial sands overlying the slope deposits is 665±55 ka. This age is older than previous chronological data placing the first European Acheulean assemblages north of 45(th) parallel north at around 500 ka and modifies our current vision of the initial peopling of northern Europe. Acheulean settlements are older than previously assumed and the oldest evidences are not only located in southern Europe. La Noira is the oldest evidence of Acheulean presence in north-western Europe and attests to the possibility of pioneering phases of Acheulean settlement which would have taken place on a Mode 1-type substratum as early as 700 ka. The lithic assemblage from la Noira thus provides behavioral and technological data on early Acheulean occupation in Europe and contributes to our understanding of the diffusion of this tradition.

San Bernardino Cave (Italy) and the appearance of Levallois technology in Europe: results of a radiometric and technological reassessment.

The introduction of Levallois technology in Europe marked the transition from the Lower to the early Middle Paleolithic. This new method of flake production was accompanied by significant behavioral changes in hominin populations. The emergence of this technological advance is considered homogeneous in the European archaeological record at the Marine isotopic stage (MIS) 9/MIS 8 boundary. In this paper we report a series of combined electron spin resonance/U-series dates on mammal bones and teeth recovered from the lower units of San Bernardino Cave (Italy) and the technological analyses of the lithic assemblages. The San Bernardino Cave has yielded the earliest evidence of Levallois production on the Italian Peninsula recovered to date. In addition to our results and the review of the archaeological record, we describe the chronological and geographical differences between European territories and diversities in terms of technological developments. The belated emergence of Levallois technology in Italy compared to western Europe corresponds to the late Italian Neanderthal speciation event. The new radiometric dates and the technological analyses of San Bernardino Cave raise the issue of the different roles of glacial refugia in the peopling and the spread of innovative flaking strategies in Europe during the late Middle Pleistocene.

Combined ESR/U-series chronology of Acheulian hominid-bearing layers at Trinchera Galería site, Atapuerca, Spain.

The Sierra de Atapuerca, northern Spain, is known from many prehistoric and palaeontological sites documenting human prehistory in Europe. Three major sites, Gran Dolina, Galería and Sima del Elefante, range in age from the oldest hominin of Western Europe dated to 1.1 to 1.3 Ma (millions of years ago) at Sima del Elefante to c.a. 0.2 Ma on the top of the Galería archaeological sequence. Recently, a chronology based on luminescence methods (Thermoluminescence [TL] and Infrared Stimulated Luminescence [IRSL]) applied to cave sediments was published for the Gran Dolina and Galería sites. The authors proposed for Galería an age of 450 ka (thousands of years ago) for the units lower GIII and GII, suggesting that the human occupation there is younger than the hominid remains of Sima de los Huesos (>530 ka) around 1 km away. In this paper, we present new results obtained by combined Electron Spin Resonance/Uranium-series (ESR/U-series) dating on 20 herbivorous teeth from different levels at the Galería site. They are in agreement with the TL results for the upper part of the stratigraphic sequence (GIV and GIIIb), in the range of between 200 and 250 ka. But for the GIIIa to GIIb levels, the TL ages become abruptly older by 200 ka while ESR ages remain relatively constant. Finally, the TL and ESR data agree in the lowest part of the section (GIIa); both fall in the range of around 350-450 ka. Our results suggest a different interpretation for the GII, GIII and GIV units of Galería and the upper part of Gran Dolina (TD10 and TD11) than obtained by TL. The ESR/U-series results are supported by a Bayesian analysis, which allows a better integration between stratigraphic information and radiometric data.

The oldest human fossil in Europe, from Orce (Spain).

The Orce region has one of the best late Pliocene and early Pleistocene continental paleobiological records of Europe. It is situated in the northeastern sector of the intramontane Guadix-Baza Basin (Granada, Andalusia, southern Spain). Here we describe a new fossil hominin tooth from the site of Barranco León, dated between 1.02 and 1.73 Ma (millions of years ago) by Electron Spin Resonance (ESR), which, in combination with paleomagnetic and biochronologic data, is estimated to be close to 1.4 Ma. While the range of dates obtained from these various methods overlaps with those published for the Sima del Elefante hominin locality (1.2 Ma), the overwhelming majority of evidence points to an older age. Thus, at the moment, the Barranco León hominin is the oldest from Western Europe.

Radiometric dating of the type-site for Homo heidelbergensis at Mauer, Germany.

The Mauer mandible, holotype of Homo heidelbergensis, was found in 1907 in fluvial sands deposited by the Neckar River 10 km southeast of Heidelberg, Germany. The fossil is an important key to understanding early human occupation of Europe north of the Alps. Given the associated mammal fauna and the geological context, the find layer has been placed in the early Middle Pleistocene, but confirmatory chronometric evidence has hitherto been missing. Here we show that two independent techniques, the combined electron spin resonance/U-series method used with mammal teeth and infrared radiofluorescence applied to sand grains, date the type-site of Homo heidelbergensis at Mauer to 609 ± 40 ka. This result demonstrates that the mandible is the oldest hominin fossil reported to date from central and northern Europe and raises questions concerning the phyletic relationship of Homo heidelbergensis to more ancient populations documented from southern Europe and in Africa. We address the paleoanthropological significance of the Mauer jaw in light of this dating evidence.

A new Lower Pleistocene archeological site in Europe (Vallparadis, Barcelona, Spain).

Here we report the discovery of a new late Lower Pleistocene site named Vallparadís (Barcelona, Spain) that produced a rich archeological and paleontological sequence dated from the upper boundary of the Jaramillo subchron to the early Middle Pleistocene. This deposit contained a main archeological layer with numerous artifacts and a rich macromammalian assemblage, some of which bore cut marks, that could indicate that hominins had access to carcasses. Paleomagnetic analysis, electron spin resonance-uranium series (ESR-US), and the biostratigraphic chronological position of the macro- and micromammal and lithic assemblages of this layer reinforce the proposal that hominins inhabited Europe during the Lower Pleistocene. The archeological sequence provides key information on the successful adaptation of European hominins that preceded the well-known fossil population from Atapuerca and succeeded the finds from Orce basin. Hence, this discovery enables us to close a major chronological gap in the early prehistory of Iberia. According to the information in this paper and the available data from these other sites, we propose that Mediterranean Western Europe was repeatedly and perhaps continuously occupied during the late Matuyama chron.

Gamma-ray spectrometric dating of late Homo erectus skulls from Ngandong and Sambungmacan, Central Java, Indonesia.

Hominid fossils from Ngandong and Sambungmacan, Central Java, Indonesia, are considered to be the most anatomically derived and youngest representatives of Homo erectus. Nondestructive gamma-ray spectrometric dating of three of these Homo erectus skulls showed that all samples underwent uranium leaching. Nevertheless, we could establish minimum age estimates of around 40ka, with an upper age limit of around 60 to 70ka. This means that the Homo erectus of Java very likely survived the Toba eruption and may have been contemporaneous with the earliest Homo sapiens in Southeast Asia and Australasia.

Paleoenvironmental analysis.

New analysis has been carried out concerning the palaeoenvironmental reconstruction of some Italian sites dating from the Middle Pleistocene to the Bronze Age. Different aspects have been investigated on each site considering the data collected. The following sites have been analyzed: Isernia La Pineta (Molise); Visogliano and Caverna degli Orsi (Tieste); Toirano Caves (Liguria); Grotta Paglicci (Gargano); Riparo del Molare (Salerno); Grotta del Cavallo (Lecce); Castellaro Lagusello (Monzambano, Mantova).