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1.
Prostate ; 83(2): 179-189, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36262059

RESUMO

BACKGROUND: The aim of this study was to evaluate modifications in proteoglycan morphology and composition in the prostatic stroma of 18-month-old gerbils after surgical castration, in association or not with an androgenic blockade. METHODS: The animals (n = 5) were sorted into groups subjected or not to antiandrogen treatment (flutamide 10 mg/kg/day) administered for the total postsurgery period and euthanized at 7- or 30-day postcastration; the control group consisted of intact animals. Tissue analysis included immunohistochemical assessment (perlecan and chondroitin sulfate) and proteoglycan morphology was analyzed by transmission electron microscopy. RESULTS: Chondroitin sulfate frequency was increased 7 days postcastration with an androgenic blockade. The presence of these carbohydrates was rare after 30 days of androgenic blockade treatment. There was a significant increase in the amount of perlecan in the prostate stroma from groups subjected to castration plus flutamide for 7 or 30 days. Ultrastructural analysis showed that the incidence of areas occupied by proteoglycans and basement membrane was altered by treatment. In addition, androgenic blockade results in changes in the amount, thickness, and morphology of these structures. At 30 days postcastration, with or without flutamide treatment, larger proteoglycans were common. CONCLUSIONS: In this study, in particular, the decrease in chondroitin sulfate after the longer period might be understood as a prostatic response to androgenic deprivation, while the high frequency and permanence of perlecan led to the assumption that its modulation could be androgen-independent. Length and form alterations in proteoglycans as well as associations among them and with the basement membrane were dynamic events in the prostate microenvironment.


Assuntos
Flutamida , Próstata , Masculino , Animais , Flutamida/farmacologia , Gerbillinae , Androgênios/farmacologia , Sulfatos de Condroitina/farmacologia , Orquiectomia
2.
Braz J Microbiol ; 53(3): 1279-1287, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35460509

RESUMO

Sexual transmission of Zika virus (ZIKV), an important arbovirus, and the virus persistence in semen raise several questions about how and where it circulates in the male reproductive system (MRS). Several studies reported detection of the virus in testes, epididymis, and prostate at 5 days post-infection (dpi) or more in animal models. In the present study, we investigated the interactions of ZIKV with mouse MRS using the AG129 strain, a ZIKV permissive immunodeficient mouse strain, at two dpi. Viral RNA was detected in blood, testes, epididymis, and prostatic complexes (prostate and seminal vesicles). Immunohistochemical (IHC) analyses, based on the envelope protein, showed an early infection in organs of MRS since ZIKV positive antigens were detected in cells within or surrounding blood vessels, Sertoli, and germ cells in testes and epithelial cells in epididymis and prostate. Positive antigens for NS5 protein, the virus RNA-dependent RNA polymerase, were also detected by IHC in these organs and circulating leukocytes, suggesting that the virus replicates in these sites as early as 2 days post-infection. Analysis of the early stages of ZIKV infection in MRS may improve the current knowledge about this issue and contribute to the development of therapies directed to the infection at this site.


Assuntos
Infecção por Zika virus , Zika virus , Animais , Genitália Masculina , Masculino , Camundongos , RNA Viral/genética , Sêmen , Zika virus/genética
3.
Parasitol Res ; 119(10): 3517-3522, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32617725

RESUMO

The parasite-vector interaction of Chagas disease is still poorly understood and the understanding of this relationship can help in the development of new strategies to control Trypanosoma cruzi transmission, which is the etiological agent of this disease. Considering the need to know if T. cruzi can cause some pathology in the reproductive system of the Chagas disease vectors, we investigated the spermatogenesis of Triatoma infestans infected by T. cruzi through histological and cytogenetic analysis. Trypanosoma cruzi Bolivia strain infection was not pathogenic for the reproductive system of T. infestans, because all the analyzed males had normal spermatogenesis, with all phases (spermatocytogenesis, meiosis and spermiogenesis) happening without any change. Thus, we demonstrated that the presence of T. cruzi Bolivia strain does not have influence in the spermatogenesis of T. infestans and we suggest that the influences on reproductive system observed for other species were a result of the action of the parasite on gametogenesis of females.


Assuntos
Doença de Chagas/transmissão , Insetos Vetores/parasitologia , Espermatogênese/fisiologia , Triatoma/parasitologia , Trypanosoma cruzi/fisiologia , Animais , Doença de Chagas/parasitologia , Interações Hospedeiro-Parasita , Insetos Vetores/fisiologia , Masculino , Triatoma/fisiologia
4.
Cell Tissue Res ; 328(3): 617-24, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17347814

RESUMO

The incidence of ciliated cells in the prostate gland of the female gerbil (Meriones unguiculatus) is uncommon and apparently becomes more frequent during androgen (testosterone cypionate) and anti-estrogen (letrozole) endocrine therapies. To evaluate the effects of such drug therapies on the induction of ciliogenesis in the glandular epithelium of female prostate glands, adult female gerbils aged 90 days were treated for 14 days with testosterone and letrozole after which their prostate glands were removed for histological, ultrastructural, and serological analyses. The cytodifferentiation of the ciliated phenotype in the alveolar epithelium became more frequent after both the testosterone and the letrozole treatments. The ciliogenesis phenomenon of the epithelial cells in the prostate gland of female gerbils thus appears to be induced by variations in the increase of androgen levels.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Cílios/fisiologia , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Terapia de Reposição Hormonal , Próstata/efeitos dos fármacos , Animais , Peso Corporal , Feminino , Gerbillinae , Hormônios Esteroides Gonadais/sangue , Terapia de Reposição Hormonal/efeitos adversos , Letrozol , Masculino , Nitrilas/efeitos adversos , Nitrilas/farmacologia , Tamanho do Órgão , Próstata/anatomia & histologia , Próstata/citologia , Testosterona/efeitos adversos , Testosterona/farmacologia , Triazóis/efeitos adversos , Triazóis/farmacologia
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