The epidemiology of traumatic brain injuries in the fastest-paced city in China: a retrospective study.

Introduction: Traumatic brain injury (TBI) seriously affects the quality of human health and the prognosis of the patient, but the epidemiological characteristics of TBI can vary among populations. Numerous changes have occurred in the epidemiological characteristics of individuals with TBI in the fast-paced city of Shenzhen, China. However, little is known about these characteristics. This study aimed to investigate the changes in TBI epidemiology, help clinicians improve medical treatment. Methods: In this retrospective cross-sectional analysis, we collected the data of 4,229 patients with TBI admitted to 20 hospitals in Shenzhen in 2017. We collected data on age, gender, cause and severity of the injury, eventual diagnosis, time from injury to admission in a neurosurgery department, and patient outcomes. Two neurosurgeons simultaneously collected the data. We compared these results with a similar study conducted in Shenzhen during the period from 1994 to 2003 to clarify and explain the changes in the epidemiological characteristics of TBI. Results: The majority of respondents were men [2,830 (66.9%)]. The mean age was 32.5 ± 21.4 years. The youngest patient was less than 1 year old, and the oldest patient was 101 years old. A total of 3,947 (93.3%) patients had a favorable outcome, 219 (5.2%) had an unfavorable outcome, and 63 (1.5%) died. The predominant external cause was falls (1,779 [42.1%]); this was the most common cause of TBI in children and older adults. Riders of electric bicycles (423 [29.0%]) were the most vulnerable to traffic accident-related injuries. Time greater than 50 h from injury to admission to a neurosurgical department had a significant effect on prognosis (p < 0.001). Conclusion: The epidemiological characteristics of TBI have changed significantly over the past 20 years. Falls, rather than traffic accidents, were the most common cause of TBI. Further research is needed to devise solutions to decrease the incidence of falls and improve the outcomes of TBI.

Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product.

BACKGROUND: Rhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris polyphylla Smith var. chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants. METHODS: DNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. polyphylla var. chinensis. RESULTS: The results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled "Chonglou" obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis. CONCLUSIONS: DNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP).

Survey of traditional Dai medicine reveals species confusion and potential safety concerns: a case study on Radix Clerodendri Japonicum.

The adulteration of herbal products is a threat to consumer safety. In the present study, we surveyed the species composition of commercial Radix Clerodendri Japonicum products using DNA barcoding as a supervisory method. A reference database for plant-material DNA-barcode was successfully constructed with 48 voucher samples from 12 Clerodendrum species. The database was used to identify 27 Radix Clerodendri Japonicum decoction piece samples purchased from drug stores and hospitals. The DNA sequencing results revealed that only 1 decoction piece (3.70%) was authentic C. japonicum, as recorded in the Dai Pharmacopeia, whereas the other samples were all adulterants, indicating a potential safety issue. The results indicate that decoction pieces that are available in the market have complex origins and that DNA barcoding is a suitable tool for regulation of Dai medicines.

[Soil water reservoir properties of green belts in the central urban area of Shanghai, China]. / 上海中心城区绿地土壤水库特征.

Taking the typical parks and public green belts in the central urban area of Shanghai for examples, water reservoir storage of soils of different types and influencing factors were investigated based on field surveys. The results showed that the total reservoir capacity of soils of the green belts in the central urban area of Shanghai was relatively low. However, the whole green belts still accumulated a large amount of water, which reached up to 1.88×107 m3. The existing water volume was 75.7% of the total reservoir capacity, while the remaining water storage was relatively low. The detention capacity and the effective storage of green belts were 31.6% and 27.2%, respectively. The dead storage was 44.5 %. Moreover, some difference existed in the water reservoirs for different vegetation types. The total water reservoir capacity of soils and the remaining water-storage space of the tree and brush lands were obviously higher than those of the grass lands. The water-detention capacity and the effective storage of the brush lands were remarkably higher than those of the tree and grass lands, but there was no remarkable difference among various vegetation types for both the existing water volume and the dead water storage. Thus, reducing soil compaction, increasing organic-matter content, improving physical properties and optimizing plant allocation of green belts would improve the water reservoir capacity effectively for the urban green belt soils.

[Identification of Seeds and Seedlings of Chinese Medicinal Materials Using DNA Barcoding Technology:A Case Study in Paris polyphylla var. yunnanensis].

Objective: To establish a novel approach based on DNA barcode sequence, so as to guarantee the quality stability of Chinese medicinal materials. Methods: Eight species of Paris plants were collected, and a standard DNA barcode library was developed by ITS loci. Furthermore, the barcodes also used to identify the seed and seedling products that purchased from the markets. Results: ITS loci can stably and accurately distinguish Paris polyphylla var. yunnanensis and its adulterants. Conclusion: The seeds and seedlings of Chinese medicinal materials need to be properly authenticated before planting,and DNA barcoding has been found to be effective for this purpose.

[HPLC Analyzed the Iridoid Glycosides of Qingyedan Commonly Used in Folk Medicine in Yunnan].

Objective: To compare the contents of iridoid glycosides in Qingyedan medicinal materials,and to provide the scientific basis for using resources of Qingyedan and rationality of original plant medicinal. Methods: The contens of three iridoid glycosides,including swertiamarin,gentiopicroside and sweroside in Qingyedan medicinal materials were determined by HPLC. Results: The constituents of 30 samples in nine species were significant difference. And the contents of iridoid glycosides in Swertia bimaculata,Swertia tenuis and Swertia pubescens were reported for the first time. Conclusion: The results show that the contents of iridoid glycosides in Qingyedan medicinal materials have a significant difference due to the different species and producing areas. Therefore, these medicinal plants should not be used as alternative medicines for clinical application. Swertiamarin and sweroside can be selected as quality control components, this method is an effective method to identify and control the quality of Qingyedan materials.

[Soil water reservoir properties and influencing factors of typical newly-established green belts of Shanghai Chenshan Botanical Garden, China.] / å ¸åž‹æ–°å»ºç»¿åœ°ä¸Šæµ·è¾°å±±æ¤ç‰©å›­åœŸå£¤æ°´åº“ç‰¹å¾åŠå ¶å½±å“å› å­.

The effects of different vegetation types, compaction ways and soil basic physico-chemical properties on soil water reservoir in the typical newly-established green belts of Shanghai Chenshan Botanical Garden were studied. The results showed that the total reservoir capacity, detention capacity and effective storage for the Botanical Garden were lower than those of natural forests. However, the dead storage was very high accounting for 60.6% of the total reservoir capacity, resulting in reduced flood storage and drainage capacity for the greens. The total reservoir capacity and detention capacity of different vegetation types were in order of brush land> tree land> grassland> bamboo land> bare land. The effective storages of the brush land and the tree land were relatively high, whereas those of the bare land and the bamboo land were lower. The ratios of the dead storage over the total re-servoir capacity in the bare land and the bamboo land were relatively high with the values 65.5% and 67.6%, respectively. The total reservoir capacity, detention capacity and effective storage of the brush land were significantly different from those of the bare land. The vegetation significantly improved the water storage and retention capacity for the soil, while the compaction by large machinery and man-caused trampling reduced the total reservoir capacity, detention capacity and effective storage of soils. The water reservoir properties were influenced by soil bulk density, saturated hydraulic conductivity, capillary porosity, non-capillary porosity, total porosity, clay and organic matter contents. Therefore, improving the soil physico-chemical properties might increase the soil reservoir capacity of the urban green belt effectively.

[Organic carbon mineralization in various size aggregates of paddy soil under aerobic and submerged conditions].

With incubation tests in laboratory, the mineralization of organic carbon in various size aggregates of paddy soil was investigated under aerobic and submerged conditions. The results showed that the organic carbon mineralization in various size aggregates decreased quickly at the beginning of the incubation, but remained stable during the late period of incubation. The mineralization rate varied significantly with the size of the aggregates. Through the incubation time, the organic carbon in 1-2 mm aggregates had the highest mineralization rate, while that in < 0.053 mm aggregates had the lowest one. Statistic analyses indicated that the mineralization rate of organic carbon in various size aggregates was significantly and linearly correlated with the contents of organic carbon and microbial biomass carbon in the aggregates. 0.25-1 mm aggregates had the highest contribution to the cumulative mineralization of soil organic carbon, accounting for 41.77% under aerobic condition and 34.11% under submerged condition, while < 0.053 mm and 1-2 mm aggregates had the lowest contribution under aerobic and submerged conditions, accounting for 7.8% and 6.6%, respectively.