RESUMO
To critically evaluate the effects of manual therapy (MT) on pain and functional improvement in patients with rotator cuff injury (RCI), a systematic review of all randomized controlled trials (RCTs) on MT for RCI was conducted in the following databases: PubMed, Cochrane Central Register of Controlled Trials, Embase, Web of Science, Physiotherapy Evidence Database, Chinese National Knowledge Infrastructure, Wan-fang Data, Chinese Scientific Journal Database, and Chinese Biomedical Literature database from inception to March 28, 2023. A total of 1,110 participants from 24 eligible RCTs were included in the analysis. Compared with placebo, MT could not effectively relieve pain [standardized mean difference (SMD)=-0.25; 95% CI: -0.51 to 0.01; P=0.06], although its impact on functional improvement appears limited (SMD=0.20; 95% CI: -0.09 to 0.49; P=0.18). Combining MT with exercise had significant advantages over exercise alone, as combined therapy contributed to both pain reduction (SMD=0.36; 95% CI: 0.08 to 0.64; P=0.01) and functional enhancement (SMD=0.32; 95% CI: 0.11 to 0.52; P=0.002). Furthermore, MT combined with multimodal physiotherapy showed additional benefits in pain reduction (mean difference=1.57; 95% CI: 0.18 to 2.96; P=0.03) and functional improvement (SMD=0.77; 95% CI: 0.43 to 1.12; P<0.0001) compared with multimodal physiotherapy alone. These findings highlight the superior pain alleviation and functional improvement provided by MT when combined with exercise or physiotherapy. Consequently, MT has emerged as a pivotal component of therapeutic intervention for RCI.
RESUMO
BACKGROUND: To comprehensively assess the neurologic recovery potential of chondroitinase ABC (ChABC) in rats after spinal cord injury (SCI). METHODS: The PubMed, Embase, ScienceDirect, Web of Science, and China National Knowledge Infrastructure databases were searched for animal experiments that evaluated the use of ChABC in the treatment of SCI up to November 2022. Studies reporting neurological function using the Basso, Beattie, and Bresnahan (BBB) scale, as well as assessments of cavity area, lesion area, and glial fibrillary acidic protein (GFAP) levels, were included in the analysis. RESULTS: A total of 46 studies were ultimately selected for inclusion. The results of the study showed that rats with SCI that received ChABC therapy exhibited a significant improvement in locomotor function after 7 days compared with controls (32 studies, weighted mean difference (WMD) = 0.58, [0.33, 0.83], p < 0.00001). Furthermore, the benefits of ChABC therapy were maintained for up to 28 days according to BBB scale. The lesion area was reduced by ChABC (5 studies, WMD = -20.94, [-28.42, -13.46], p < 0.00001). Meanwhile, GFAP levels were reduced in the ChABC treatment group (8 studies, WMD = -29.15, [-41.57, -16.72], p < 0.00001). Cavity area is not statistically significant. The subgroup analysis recommended that a single injection of 10 µL (8 studies, WMD = 2.82, [1.99, 3.65], p < 0.00001) or 20 U/mL (4 studies, WMD = 2.21, [0.73, 3.70], p = 0.003) had a better effect on improving the function. The funnel plot of the BBB scale was found to be essentially symmetrical, indicating a low risk of publication bias. CONCLUSIONS: This systematic review and meta-analysis has indicated that ChABC could improve functional recovery in rats after SCI.
RESUMO
Noble metal nanoclusters (<2 nm) prepared using special molecules such as proteins and peptides as templates have been attracting increasing attention in biomedical applications and cell imaging because of their excellent biocompatibility, size-dependent fluorescence properties, and relatively simple synthesis. Although cell imaging using metal clusters has developed rapidly in recent years, multimodal imaging of the cell nucleus remains challenging. Herein, we report the successful synthesis of two different types of gold nanoclusters, one with blue fluorescence emission (λex 320 nm, λem 405 nm) and the other with red fluorescence emission (λex 560 nm, λem 657 nm), using the same polypeptide SV (sequence: NH2-CCYGGPKKKRKVG-COOH) as a template. The synthesis of these two optically distinct Au nanoclusters (Au NCs) was achieved through the optimization of synthesis conditions, including light, pH, the Au: polypeptide ratio, reaction time, and temperature. The multimodal imaging ability of the Au NCs were subsequently explored, with particular emphasis on the enzyme-like catalysis of the NCs and their use in the optical imaging of cell nuclei. Results show that the Au NCs possessed similar biological activities as the parent peptide used in their synthesis as well as demonstrating peroxidase-like activity due to the Au nanocluster core. Further, the Au NCs targeted the cell nucleus, with their strong fluorescence allowing cell imaging (cells containing Au clusters could be distinguished by eye, eliminating the need for additional labels). Finally, the Au NCs exhibited excellent multimodal cell imaging abilities. The results suggest that the Au NCs introduced here are promising candidates for the labeling and analysis of pathological samples.