Stable expression of red fluorescent protein-blasticidin deaminase fusion gene (rfp-bsd) as a selectable marker for DNA transfection in Babesia ovata.

The red fluorescent protein(rfp)-blasticidin deaminase(bsd) fusion gene was transfected into Babesia ovata by electroporation with the plasmid DNA and selected with 15 µg/mL of blasticidin S under the in vitro culture condition. The transfected parasite with episomal DNA was selected and cultured for further analysis based on the presence of the rfp-bsd fusion gene by PCR and expression of the fusion protein by immunofluorescence antibody test under fluorescence microscopy for 2 months after the transfection. The results are the first, to our knowledge, to demonstrate the expression and stability of the episomal rfp-bsd fusion gene under the control of actin promoter as a selectable marker for the transfection system in B. ovata.

Molecular identification, risk factor assessment, and phylogenetic analysis of tick-borne pathogens in symptomatic and asymptomatic cattle from South-Eastern Iran.

Tick-borne pathogens (TBPs) represent a substantial threat to cattle globally, exerting adverse impacts on production, health, and economic viability. This study delves into the prevalence and implications of TTBPs in cattle sourced from resource-limited smallholder livestock farms situated in southeastern Iran, proximate to Afghanistan and Pakistan. Blood and tick specimens were systematically collected from a cohort of 230 cattle, comprising 150 asymptomatic and 80 symptomatic individuals. Genomic DNA isolated from blood samples underwent rigorous examination for the presence of key TBPs, including Anaplasma marginale, A. phagocytophilum, A. bovis, A. centrale, Babesia bigemina, and Theileria annulata, utilizing multiple genetic markers. Nucleotide sequence analysis facilitated the reconstruction of phylogenetic relationships. The study also evaluated various potential risk factors, such as clinical status, gender, age, breed, tick infestation, and management practices, to elucidate their associations with TTBPs. Among the cattle cohort, a staggering 87.8% (202/230) tested positive for at least one pathogen. Prevalence statistics encompassed A. marginale (72.2%), T. annulata (68.3%), A. phagocytophilum/A. platys-like complex (66.1%), A. centrale (16.7%), B. bigemina (10.0%), and A. bovis (6.1%). Remarkably, mixed infections involving two, three, and four pathogens were detected in 23%, 52.1%, and 2.2% of animals, respectively. Notably, all asymptomatic cattle were positive for at least one TBP. Tick infestation was observed in 62.2% (143/230) of cattle, predominantly caused by Hyalomma anatolicum (82.5%), Rhipicephalus (Boophilus) annulatus (13.1%), and R. sanguineus sensu lato (4.4%). Risk factors linked to TBPs encompassed tick infestation, older age, and crossbred animals. Clinical presentations among symptomatic cattle encompassed fever, anemia, weight loss, anorexia, jaundice, and enlarged superficial lymph nodes. This study underscores the pivotal role of asymptomatic carriers in the propagation of TTBPs within endemic regions. Furthermore, it emphasizes the potential for the implementation of molecular diagnostics to unmask subclinical infections, thereby affording the opportunity for targeted interventions aimed at ameliorating the burden of TTBPs in resource-constrained smallholder dairy farms.

Thermal neutron beam optimization for PGNAA applications using Q-learning algorithm and neural network.

As a powerful, non-destructive analysis tool based on thermal neutron capture reaction, prompt gamma neutron activation analysis (PGNAA) indeed requires the appropriate neutron source. Neutrons produced by electron Linac-based neutron sources should be thermalized to be appropriate for PGNAA. As a result, thermalization devices (TDs) are used for the usual fast neutron beam to simultaneously maximize the thermal neutron flux and minimize the non- thermal neutron flux at the beam port of TD. To achieve the desired thermal neutron flux, the optimized geometry of TD including the proper materials for moderators and collimator, as well as the optimized dimensions are required. In this context, TD optimization using only Monte Carlo approaches such as MCNP is a multi-parameter problem and time-consuming task. In this work, multilayer perceptron (MLP) neural network has been applied in combination with Q-learning algorithm to optimize the geometry of TD containing collimator and two moderators. Using MLP, both thickness and diameter of the collimator at the beam port of TD have first been optimized for different input electron energies of Linac as well as for moderators' thickness values and the collimator. Then, the MLP has been learned by the thermal and non-thermal neutron flux simultaneously at the beam port of TD calculated by MCNPX2.6 code. After selecting the optimized geometry of the collimator, a combination of Q-learning algorithm and MLP artificial neural network have been used to find the optimal moderators' thickness for different input electron energies of Linac. Results verify that the final optimum setup can be obtained based on the prepared dataset in a considerably smaller number of simulations compared to conventional calculation methods as implemented in MCNP.

Combining CNN and Q-learning for increasing the accuracy of lost gamma source finding.

The increasing use of nuclear technology in various fields makes it necessary to provide the required safety to work with this industry. Gamma source is one of the most widely used sources in industry and medicine. Finding a lost gamma source in a gamma irradiation room without human presence is challenging due to the particular arrangements and barriers in the room for radiation shielding and requires an efficient and robust method. In this paper, locating and routing the lost gamma source in the gamma irradiation room containing radiation blocking barriers are done simultaneously by using two methods, convolutional neural network (CNN) and Q-learning, which are powerful algorithms for deep learning and machine learning. Environment simulation with gamma source was performed using Geant4 simulation. The results show that by combining these two methods in geometries with radiation blocking barriers, in addition to locating with 90% accuracy, routing can also be performed. Although the presence of thick barriers in the room reduces the accuracy, increases the time required to finding the lost gamma source or the inefficiency of other methods, nevertheless, the results show that combination of CNN and Q-learning reduces the time and greatly increases the accuracy.

Polytraumatization in young male refugees from the Middle East and its association with internalizing and externalizing symptoms.

BACKGROUND: Young Middle Eastern male refugees are currently among the most vulnerable groups in Europe. Most of them have experienced potentially traumatic events (PTEs) such as rape, torture, or violent assaults. Compared to their peers, young refugees suffer more from internalizing and externalizing symptoms, especially when unaccompanied. Little is known about the cumulative impact of experiencing different types of PTEs on mental health outcomes (polytraumatization) of young male refugees from the Middle East. We investigated (1) whether there is a dose-response relationship between multiple PTE types experienced and mental health outcomes, (2) whether individual types of PTEs are particularly important, and (3) to what extent these are differentially associated with mental health outcomes among unaccompanied or accompanied peers. METHODS: In total, 151 young Middle Eastern male refugees in Germany (Mage = 16.81 years, SDage = 2.01) answered questionnaires on PTEs, mental health, and post-migration stress. RESULTS: Hierarchical regression analyses revealed, while controlling for age, duration of stay, unaccompanied status, and post-migration stress, (1) a dose-effect between PTE types on both internalizing and externalizing symptoms. Moreover, (2) regarding internalizing symptoms, violent family separation and experiencing life-threatening medical problems were particularly crucial. The latter was driven by unaccompanied refugees, who also reported higher levels of substance use. CONCLUSIONS: The results extend findings from the literature and suggest that not only may greater polytraumatization be related to greater depression among refugees, but also to a range of other mental health outcomes from the internalizing and externalizing symptom domains. Furthermore, the results highlight the mental health risks that unaccompanied and accompanied refugee adolescents face after exposure to PTEs, and provide information for practitioners as well as researchers about event types that may be particularly relevant.

Are intestinal helminths playing a positive role in tuberculosis risk? A systematic review and meta-analysis.

BACKGROUND: Co-infection of intestinal helminthic infections (IHIs) and tuberculosis (TB) has appeared as a public health issue, especially in developing countries. Some recent studies have been carried out on the possible relevance of IHIs to TB. The current systematic review and meta-analysis was conducted to assess the prevalence and odds ratio (OR) of IHIs among TB patients and clarify the relationship between IHIs and TB disease. METHODS: For the purpose of the study, five English databases including PubMed, Science Direct, Scopus, Web of Science (ISI), and Google scholar were searched (up to January 30, 2019) in order to find the related studies. Random-effects meta-analysis model was used to estimate the pooled prevalence, odds ratio (OR), and 95% confidence interval (CI). Inclusion and exclusion criteria were applied. RESULTS: A total of 20 studies including 10 studies with case-control design (2217 patients and 2520 controls) and 10 studies with cross-sectional design (a total of 2415 participants) met the eligibility criteria. As shown by the random-effects model, the pooled prevalence of IHIs in TB patients was estimated to be 26% (95% CI, 17-35%; 1249/4632). The risk of IHI was higher in TB patients compared to controls but this was not statistically significant. However, according to genus/species, the pooled OR of Strongyloides stercoralis (S. stercoralis) (OR, 2.68; 95% CI, 1.59-4.54) had a significantly higher risk in TB patients compared to controls. Nevertheless, the results of random effects model showed no statistically significant association between overall pooled OR of IHIs in TB patients compared to controls in case-control studies (OR, 1; 95% CI, 0-1). CONCLUSIONS: It is highly recommended that more precise studies should be carried out by researchers in order to better understand this association. Also, it is of great importance to include the periodic screenings for IHIs in the routine clinical care of these patients.

Highlights of human ectopic fascioliasis: a systematic review.

Fascioliasis is a tropical zoonotic disease caused by the Fasciola parasite. The adult parasite usually resides in the liver and biliary ducts; however, several cases of ectopic fascioliasis (EF) have been reported. This study is a highlight on EF according to the confirmed case reports. In a setting of systematic review, we found 25 eligible articles containing 26 confirmed cases of EF (any date until 30 November 2018), including abdominal and intestinal EF in six cases, skin and subcutaneous tissues in five cases, eye in four cases, brain and pancreas in three cases, neck and lymph node in two cases, and lung, dorsal spine, and peritoneal cavity in one case, respectively. The result indicates that fascioliasis can have diverse ectopic forms and should be more attended in the endemic regions of fascioliasis in order to distinguish from other endemic diseases.

A Brief Introduction to the Multidimensional Intercultural Training Acculturation Model (MITA) for Middle Eastern Adolescent Refugees.

The large number of adolescent refugees around the world constitutes a great challenge for societies. However, current models of acculturation have been developed for migrants, but not specifically for adolescent refugees. Crucial factors to describe adolescent refugee acculturation, such as intentions to return to their homeland, especially with respect to adolescent refugees with temporary residency and experiences of potentially traumatic events, are missing. Hence, the Multidimensional Intercultural Training Acculturation (MITA) model is introduced. The model proposes that two major concerns for adolescent refugees, which are socio-cultural adjustment and mental health, are predicted by intercultural and social⁻emotional competence, intentions to return to their homeland, and experiences of traumatic events. Moreover, the effects of three modes of acculturation are also proposed in the model. It is expected that these variables mediate the effects of intercultural competence, social⁻emotional competence, intentions to return to the homeland, and experiences of traumatic events on socio-cultural adjustment as well as mental health. Finally, it is also expected that in-group social support and out-group social support moderate the direct connection between the experiences of traumatic events and mental health.

Promoting Mental Health in Unaccompanied Refugee Minors: Recommendations for Primary Support Programs.

During the last years, the number of refugees around the world increased to about 22.5 million. The mental health of refugees, especially of unaccompanied minors (70% between the ages of 16 and 18 years) who have been exposed to traumatic events (e.g., war), is generally impaired with symptoms of post-traumatic stress disorder, depression, and anxiety. Several studies revealed (1) a huge variation among the prevalence rates of these mental problems, and (2) that post-migration stressors (e.g., language barriers, cultural differences) might be at least as detrimental to mental health as the traumatic events in pre- and peri-flight. As psychotherapy is a limited resource that should be reserved for severe cases and as language trainings are often publicly offered for refugees, we recommend focusing on intercultural competence, emotion regulation, and goal setting and goal striving in primary support programs: Intercultural competence fosters adaptation by giving knowledge about cultural differences in values and norms. Emotion regulation regarding empathy, positive reappraisal, and cultural differences in emotion expression fosters both adaptation and mental health. Finally, supporting unaccompanied refugee minors in their goal setting and goal striving is necessary, as they carry many unrealistic wishes and unattainable goals, which can be threatening to their mental health. Building on these three psychological processes, we provide recommendations for primary support programs for unaccompanied refugee minors that are aged 16 to 18 years.

A qualitative study of cognitive-behavioral therapy for Iranian migrants with mild/moderate depression in Austria.

This qualitative study was carried out to investigate the effectiveness of cognitive-behavioral therapy (CBT) for Iranian migrants suffering from major depressive disorder (MDD) and living in Austria for an average of 14 years. The qualitative data were collected through interviews based on the Farsi version of the Structured Clinical Interview for the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV). However, to obtain more information from the participants, they were asked to talk in more detail about their childhood and teenage years, reasons for immigration, their lifestyle before and after immigration, and their social activities. Interviews were conducted at four time points: preintervention, postintervention, 1-month follow-up, and 6-month follow-up. Patients who did not complete the interventions were interviewed on a voluntary basis to explain their reasons. Preintervention interviews were conducted to get some useful information about participant's' expectations of psychotherapy, especially group-based cognitive-behavioral therapy (GCBT), and also to assess the reasons for depression from their own point of view. The postintervention interviews were conducted to examine the participants' psychological situations as well as the reasons for positive effects of interventions. The interviews on average lasted 50 minutes, and field notes were taken. The results of this study showed a reduction in depression symptoms after the interventions. However, the effect of treatment was not persistent. The findings suggest that the main reasons behind Iranian migrants' depression in Austria is related to their dysfunctional acculturation attitude. The effectiveness of GCBT for Iranian migrants with depression also may be related to their sociocultural background.