Inorganic Characterization of Feeds Based on Processed Animal Protein Feeds.

The potential of utilizing inorganic constituents in processed animal proteins (PAPs) for species identification in animal feeds was investigated, with the aim of using these constituents to ensure the quality and authenticity of the products. This study aimed to quantify the inorganic content across various PAP species and assess whether inorganic analysis could effectively differentiate between PAP species, ultimately aiding in the identification of PAP fractions in animal feeds. Four types of PAPs, namely bovine, swine, poultry, and fish-based, were analyzed and compared to others made up of feathers of vegetal-based feed. Also, three insect-based PAPs (Cricket, Silkworm, Flour Moth) were considered in this study to evaluate the differences in terms of the nutrients present in this type of feed. Ionic chromatography (IC) was used to reveal the concentrations of NO3-, NO2, Cl-, and SO42-, and inductively coupled plasma optical emission spectroscopy (ICP-OES) to detect Al, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, P, Si, Sr, Ti, and Zn. The application of multivariate chemometric techniques to the experimental results allowed us to determine the identification capability of the inorganic composition to identify correlations among the variables and to reveal similarities and differences among the different species. The results show the possibility of using this component for discriminating between different PAPS; in particular, fish PAPs are high in Cd, Sr, Na, and Mg content; swine PAPs have lower metal content due to high fat; feathers and vegetal feed have similar Al, Si, and Ni, but feathers are higher in Fe and Zn; and insect PATs have nutrient levels comparable to PAPs of other origins but are very high in Zn, Cu, and K.

Verbal fluency patterns associated with the amnestic conversion from mild cognitive impairment to dementia.

Patients with amnestic mild cognitive impairment (aMCI) are at a higher risk of converting to Alzheimer's disease. The aim of this study was to examine the potential use of Verbal Fluency (VF) measures as markers for predicting the conversion to dementia. At baseline, 61 aMCI, aged 65 to 80 years, underwent a comprehensive neuropsychological assessment, including phonemic (PVF) and semantic verbal fluency (SVF) tasks. After 18 months, 14 individuals with aMCI had progressed to a diagnosis of dementia. The findings revealed that aMCI-converter group had lower Mini Mental State Examination and Rey Auditory Verbal Learning Task scores than aMCI-no converter and produced fewer clusters in both VF tasks and a lower number of switches in PVF at baseline (p < 0.05). According to receiver operating characteristic curve analysis, the number of clusters in PVF had the highest predictive value (AUC = 0.80) with a threshold of 5.510 for identifying aMCI-converter at baseline. Additionally, participants with higher levels of education exhibited more clusters and switches in VF tasks (p < 0.05). These results suggest that qualitative measures of VF could serve as neuropsychological markers for predicting cognitive decline in individuals with aMCI. Furthermore, the study highlights the potential influence of the education level on cognitive performance in neuropsychological tasks.

Advancements in Portable Voltammetry: A Promising Approach for Iron Speciation Analysis.

Iron, a crucial element in our environment, plays a vital role in numerous natural processes. Understanding the presence and concentration of iron in the environment is very important as it impacts various aspects of our planet's health. The on-site detection and speciation of iron are significant for several reasons. In this context, the present work aims to evaluate the applicability of voltammetry for the on-site determination of iron and its possible speciation using a portable voltammetric analyzer. Voltammetry offers the advantage of convenience and cost-effectiveness. For iron (III) determination, the modification of a glassy carbon electrode (GCE) with an antimony-bismuth film (SbBiFE) using the acetate buffer (pH = 4) as a supporting electrolyte was used. The technique adopted was Square Wave Adsoptive Cathodic Stripping Voltammetry (SW-AdCSV), and we used 1-(2-piridylazo)-2-naphthol (PAN) as the iron (III) ligand. Linearity, repeatability, detection limit, and accuracy were determined using synthetic solutions; then, a Standard Reference Material (SRM) of 1643f Trace Elements in Water (iron content: 93.44 ± 0.78 µg L-1) was used for validation measurements in the real matrix. the accuracy of this technique was found to be excellent since we obtained a recovery of 103.16%. The procedure was finally applied to real samples (tap, lake, and seawater), and the results obtained were compared via Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES). The amount of iron found was 207.8 ± 6.6 µg L-1 for tap water using voltammetry and 200.9 ± 1.5 µg L-1 with ICP-OES. For lake water, 171.7 ± 3.8 µg L-1, 169.8 ± 4.1 µg L-1, and 187.5 ± 5.7 µg L-1 were found using voltammetry in the lab both on-site and using ICP-OES, respectively. The results obtained demonstrate the excellent applicability of the proposed on-site voltammetric procedure for the determination of iron and its speciation in water.

Strategies for mercury speciation with single and multi-element approaches by HPLC-ICP-MS.

Mercury (Hg) and its compounds are highly toxic for humans and ecosystems, and their chemical forms determine both their behavior and transportation as well as their potential toxicity for human beings. Determining the various species of an element is therefore more crucial than understanding its overall concentration in samples. For this reason, several studies focus on the development of new analytical techniques for the identification, characterization, and quantification of Hg compounds. Commercially available, hyphenated technology, such as HPLC-ICP-MS, supports the rapid growth of speciation analysis. This review aims to summarize and critically examine different approaches for the quantification of mercury species in different samples using HPLC-ICP-MS. The steps preceding the quantification of the analyte, namely sampling and pretreatment, will also be addressed. The scenarios evaluated comprehend single and multi-element speciation analysis to create a complete guide about mercury content quantification.

Semantic Self-Images and Well-Being in Young and Older Adults: Does the Accessibility Matter?

In the present study we investigated whether and how age group, dimensions of well-being and their interactions predicted the phenomenological properties of semantic self-images, taking also into account the different levels of accessibility of self-images (i.e., order of generation). Results on the first self-image revealed that, independently of age, higher levels of life satisfaction predicted higher likelihood of positive than negative statement and higher levels of negative affect and life satisfaction predicted higher levels of personal relevance of the self-image. When all self-images were considered, for higher levels of life satisfaction neutral and positive self-images were more likely than negative ones, and for lower levels of positive affect, neutral images were more likely than negative ones. Moreover, young adults were more likely than older adults to report neutral rather than negative self-images and, for higher levels of positive affect, they were more likely to report neutral and positive images instead of negative ones. These results suggest that the accessibility of semantic self-images should be taken into account in the investigation of the complex association between well-being and semantic self-images. Theoretical and practical implications of these results are discussed.

On-Site Determination of Methylmercury by Coupling Solid-Phase Extraction and Voltammetry.

A measurement and speciation procedure for the determination of total mercury (HgTOT), inorganic mercury (HgIN), and methylmercury (CH3Hg) was developed and the applicability for on-site determination was demonstrated. A simple, portable sample pretreatment procedure was optimized to extract the analytes. Home-made columns, packed with a new sorbent material called CYXAD (CYPHOS 101 modified Amberlite XAD), were used to separate the two forms of the analyte. HgTOT and CH3Hg were determined by anodic stripping voltammetry (ASV), using a solid gold electrode (SGE). Two certified reference materials (BCR-463 Tuna Fish and Tuna Fish ERM-CE 464) and eight fresh fishes were analyzed. Then, the results that were obtained following the optimized portable procedure were compared with the concentrations obtained, using a direct mercury analyzer (DMA). This quantification, using the two techniques, demonstrated the good performance of the proposed method.

Web-Based Assessment of the Phenomenology of Autobiographical Memories in Young and Older Adults.

Autobiographical memories (ABMs) produce rich phenomenological experiences. Although few standardized and comprehensive measures of the phenomenology of ABMs have been developed, a web-based assessment of the full range of phenomenological properties is still missing. In the present study, we aimed to fill this gap and tested the psychometric properties of a web-based version of the Assessment of the Phenomenology of Autobiographical Memory (APAM) in a group of young and older adults. Specifically, taking advantage of the flexibility of web-based assessment methodology, we tested the rating consistency of APAM items, asking participants to rate the phenomenology of their ABMs with respect to seven cues, administered in one per day in seven different days. In each session, we also collected ratings of mood and arousal. Using linear mixed modeling (LMM), we could examine whether the phenomenology ratings differed with respect to age group while controlling for sex, age of the memory, arousal, mood, and specificity of the memory. Results revealed an adequate level of consistency of ratings in both young and older adults. Moreover, LMMs revealed a more intense experience of recollection and reliving (i.e., sensory and emotional) and a higher confidence in memory accuracy in older compared to younger adults. The theoretical and practical usefulness of a web-based assessment of the phenomenology of ABMs are discussed.