Fiber degradation and carbohydrate production by combined biological and chemical/physicochemical pretreatment methods of lignocellulosic biomass - A review.

Sustainable biorefinery concepts based on lignocellulosic biomass are gaining worldwide research interest because of their inexpensiveness and abundance. The recalcitrance of lignocellulosic biomass poses a major hindrance to enhance biofuel production. Therefore, a pretreatment step is critical to prepare the substrates for the downstream process. Combining pretreatment steps help to lower the severity of the drawbacks of a single pretreatment step. This paper systematically reviews the combined biological and chemical/physicochemical pretreatment based on fiber degradation and sugar yield. An energy-efficient biological pretreatment method combined with a chemical pretreatment that accelerates the pretreatment times has been seen to be efficient for fiber degradation and sugar yields. However, fungal species, culture conditions, biomass type, the severity of chemical pretreatment and the order of sequential pretreatment influences the relative component contents and sugar yield. Even the same biomass from different sources undergoing similar pretreatment conditions could result in a varying amount of digestibility.

Amphiphilic azobenzenes: Antibacterial activities and biophysical investigation of their interaction with bacterial membrane lipids.

With the emergence of multi-drug resistant bacteria and hospital-acquired infections, there is an urgent need to develop new antibiotics. Here, we report the synthesis, physico-chemical characterizations, and antimicrobial activity assays of four Azo compounds that differ in their alkyl chain length. The molecular mechanism of their antibacterial activity was investigated by complementary in vitro and in silico biophysical studies. The compounds with alkyl chain lengths of four or six carbons showed a low MIC50 against Escherichia coli and Bacillus subtilis. Our investigations into the mechanism of their action revealed that phosphatidylethanolamine in the bacterial plasma membrane plays an important role in their antibacterial activity.