RESUMO
Sphingolipidoses are a group of metabolic diseases in which lysosomal hydrolases dysfunction disrupt normal sphingolipids' metabolism, leading to excess accumulation in cellular compartments and excretion in urine. These pathologies represent a significant burden among Moroccan population, for which an easy access to enzymatic assays and genetic tests is not guaranteed. Parallel analytical methods thus have to be developed for preliminary screening. In this study, 107 patients were addressed to the metabolic platform of the Marrakesh Faculty of Medicine for diagnosis confirmation. Thin-Layer Chromatography was used as a first step to perform chemical profiling of the patients' urinary lipids, allowing 36% of the patients to be efficiently oriented towards the adequate enzymatic assay. UPLC-MS/MS analyses of urinary sulfatides excreted in urines patient had been used to control the reliability of TLC analysis and to obtain more accurate information related to the sulfatides isoforms. This analytical process combining TLC with UPLC-MS/MS has enabled rapid and appropriate patient management in a reduced time and with reduced resources.
Assuntos
Esfingolipidoses , Sulfoglicoesfingolipídeos , Humanos , Cromatografia Líquida/métodos , Marrocos , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/métodos , Esfingolipidoses/diagnósticoRESUMO
The antibacterial activity of different extracts of Quercus ilex bark (Fagaceae) was studied in vitro against seven reference strains of bacteria by using a disc-diffusion method and agar-dilution method. The ethyl acetate extract (QE), n-butanol extract (QB) and final aqueous layer (QA) were effective against all bacterial strains tested at MICs ranging from 128 to 512 microg/ml. The n-hexane extract (QH) and dichloromethane extract (QD) showed no activity.
Assuntos
Antibacterianos/farmacologia , Casca de Planta/química , Extratos Vegetais/farmacologia , Quercus/química , Antibacterianos/química , Antibacterianos/isolamento & purificação , Bacillus subtilis/efeitos dos fármacos , Bacillus subtilis/crescimento & desenvolvimento , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/métodos , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/crescimento & desenvolvimento , Marrocos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/crescimento & desenvolvimento , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/crescimento & desenvolvimento , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/crescimento & desenvolvimento , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento , Staphylococcus epidermidis/efeitos dos fármacos , Staphylococcus epidermidis/crescimento & desenvolvimento , Streptococcus agalactiae/efeitos dos fármacos , Streptococcus agalactiae/crescimento & desenvolvimento , Streptococcus pneumoniae/efeitos dos fármacos , Streptococcus pneumoniae/crescimento & desenvolvimento , Vibrio cholerae/efeitos dos fármacos , Vibrio cholerae/crescimento & desenvolvimentoRESUMO
The essential oil of Pulicaria odora, a Moroccan medicinal plant; was analyzed by GC-MS, and subjected to column chromatography on silica gel. Two major constituents were isolated and identified as 2-isopropyl-4-methylphenol (1) and isobutyric acid 2-isopropyl-4-methylphenylester (2), by analysis of spectroscopic data (MS, 1H NMR, 13C NMR, DEPT, COSY, HMQC and HMBC experiments). The isolated compounds are reported for the first time from Pulicaria genus. The essential oil and its major constituents (compounds 1 and 2) were examined for antibacterial and antifungal activity in vitro using the diffusion and dilution methods. Results showed that the essential oil and the 2-isopropyl-4-methylphenol (1) exhibited a very significant antibacterial and antifungal activity, while the isobutyric acid 2-isopropyl-4-methylphenylester (2) was inactive for all tested strains.
