RESUMO
Human liposomal recombinant alpha 2b-interferon topically applied to laboratory animals was tested for antiviral activity, pharmacokinetics, and toxicity. The interferon was shown to penetrate through the skin and to circulate in the blood of experimental animals longer than its injectable form, and to exhibit its antiviral activity against genital herpes in guinea pigs. It produced no toxic, skin-irritant, or allergic effects in laboratory animals.
Assuntos
Antivirais/farmacologia , Herpes Genital/tratamento farmacológico , Interferon-alfa/farmacologia , Administração Cutânea , Animais , Antivirais/administração & dosagem , Antivirais/farmacocinética , Antivirais/toxicidade , Células Cultivadas/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Cobaias , Humanos , Interferon alfa-2 , Interferon-alfa/administração & dosagem , Interferon-alfa/farmacocinética , Interferon-alfa/toxicidade , Lipossomos , Masculino , Camundongos , Ratos , Ratos Wistar , Proteínas Recombinantes , Absorção CutâneaRESUMO
The effects of ointment containing king crab (Paralithodes camtschatica) collagenase on intact skin, thermal, and pyonecrotic wounds were studied in rats by using hematological, biochemical, immunological, and morphological methods. The ointment for the skin and viscera was shown to be safe. It is highly effective in debriding the infected wounds. Different concentrations of collagenase were tested. The concentration of collagenase was recommended to be 0.2 mg/g ointment for use.
Assuntos
Anti-Infecciosos Locais/administração & dosagem , Braquiúros/enzimologia , Colagenases/administração & dosagem , Cicatrização/efeitos dos fármacos , Animais , Anti-Infecciosos Locais/efeitos adversos , Anti-Infecciosos Locais/isolamento & purificação , Colagenases/efeitos adversos , Colagenases/isolamento & purificação , Modelos Animais de Doenças , Masculino , Pomadas , Ratos , Ratos Endogâmicos Lew , Segurança , Resultado do Tratamento , Infecção dos Ferimentos/tratamento farmacológico , Infecção dos Ferimentos/patologiaRESUMO
Toxic properties of recombinant human tumor necrosis factor (TNF-beta) were studied on noninbred albino mice. In the maximum tolerable doses the preparation induced a decrease in the body weight and temperature of the animals as well as development of glyco- and leukopenic reactions and damage of the internal organ structures. The preparation effects were observed early after the exposure and were mainly reversible. The most TNF-beta sensitive organs were the liver, lungs, adrenal gland, thymus and spleen. The major link in the pathological process development under the effect of the TNF-beta toxic doses was likely increased intravascular blood coagulability evident from a marked procoagulant activity of the preparation.