RESUMO
We developed a new method of creation of tissue engineering constructs for regeneration of the bone tissue based on the principle of free distribution of cells in a fibrin clot within a scaffold. The tissue engineering construct includes multipotent stromal adipose tissue cells committed in osteogenic lineage, platelet-rich plasma, and resorbed material on the basis of xenogeneic bone collagen. The culture of bone progenitor cells was characterized by the main markers of osteoblastic differon. The material meets all requirements for materials intended for tissue engineering. An innovative high-technological tissue engineering product for clinical application is prepared.
Assuntos
Gordura Abdominal/citologia , Matriz Óssea , Regeneração Óssea , Células-Tronco Multipotentes/fisiologia , Animais , Calcificação Fisiológica , Adesão Celular , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Fibrina/química , Expressão Gênica , Células-Tronco Multipotentes/metabolismo , Células-Tronco Multipotentes/transplante , Osteocalcina/genética , Osteocalcina/metabolismo , Osteopontina/genética , Osteopontina/metabolismo , Plasma Rico em Plaquetas/citologia , Coelhos , Estatísticas não Paramétricas , Engenharia Tecidual , Alicerces TeciduaisRESUMO
Stromal cells of adipose tissue and human umbilical cord were isolated by the original method from general populations of multipotent subpopulation of multipotent stromal cells exhibiting perivascular phenotype (CD146+, CD31-). Effective directed differentiation of these cells into insulin-producing cells by transient transfection of the gene Pdx1 was demonstrated. Transfection multipotent stromal cells CD146-, CD31- derived from adipose tissue and umbilical cord and isolated by the standard method, did not result in activation of insulin gene transcription. It was shown that the expression of nestin was not necessary for effective pancreatic cell differentiation.