Use of Hair as Matrix for Trace Elements Biomonitoring in Cattle and Roe Deer Sharing Pastures in Northern Italy.

Intensive cattle breeding's environmental challenges are prompting shifts to extensive, pasture-based systems, influencing nutrient and pollutant uptake. PTEs are essential and non-essential elements, regularly found in the environment and organisms, and in which unbalances lead to health issues. Hair analysis, a non-invasive method, provides retrospective PTE exposure evaluation. This study aims to understand exposure and species-specific accumulation patterns of PTEs in cattle and roe deer sharing pastures in Northern Italy using the hair analysis. Aluminum, As, Cd, Cr, Ni, Pb, Cu, Mg, Fe, and Zn were quantified through the use of ICP-OES. Findings show As levels significantly higher in roe deer due to their selective feeding, while Cd and Pb levels align with other studies. Essential elements like Cu, Fe, and Zn are lower in cattle, possibly due to diet differences. Higher Cr and Ni levels in cattle suggest contamination or physiological differences in accumulation patterns. In conclusion, hair analysis is valuable for monitoring environmental PTE exposure, highlighting significant interspecies differences and the potential of both animals as bioindicators in shared grazing areas.

Bovine Colostrum Supplementation in Rabbit Diet Modulates Gene Expression of Cytokines, Gut-Vascular Barrier, and Red-Ox-Related Molecules in the Gut Wall.

Rabbits, pivotal in the EU as livestock, pets, and experimental animals, face bacterial infection challenges, prompting a quest for alternatives to curb antibiotic resistance. Bovine colostrum (BC), rich in immunoregulatory compounds, antimicrobial peptides, and growth factors, is explored for disease treatment and prevention. This study assesses BC diet supplementation effects on rabbit intestines, examining gene expression. Thirty female New Zealand White rabbits at weaning (35 days) were divided into three experimental groups: control (commercial feed), 2.5% BC, and 5% BC. The diets were administered until slaughtering (81 days). BC-upregulated genes in the jejunum included IL-8, TGF-ß, and CTNN-ß1 at 5% BC, while PLVAP at 2.5% BC. Antioxidant-related genes (SOD1, GSR) were downregulated in the cecum and colon with 2.5% BC. BC 5% promoted IL-8 in the jejunum, fostering inflammation and immune cell migration. It also induced genes regulating inflammatory responses (TGF-ß) and gastrointestinal permeability (CTNN-ß1). BC 5% enhanced antioxidant activity in the cecum and colon, but no significant impact on anti-myxo antibody production was observed. These results suggest that BC has significant effects on the rabbit gastrointestinal tract's inflammatory and antioxidant response, but further research is required to fully understand its histological and physiological impact.