RESUMO
Site-directed mutagenesis of human serum albumin was used to study the role of various amino acid residues in bilirubin binding. A comparison of thermodynamic, proteolytic, and x-ray crystallographic data from previous studies allowed a small number of amino acid residues in subdomain 2A to be selected as targets for substitution. The following recombinant human serum albumin species were synthesized in the yeast species Pichia pastoris: K195M, K199M, F211V, W214L, R218M, R222M, H242V, R257M, and wild type human serum albumin. The affinity of bilirubin was measured by two independent methods and found to be similar for all human serum albumin species. Examination of the absorption and circular dichroism spectra of bilirubin bound to its high affinity site revealed dramatic differences between the conformations of bilirubin bound to the above human serum albumin species. The absorption and circular dichroism spectra of bilirubin bound to the above human serum albumin species in aqueous solutions saturated with chloroform were also examined. The effect of certain amino acid substitutions on the conformation of bound bilirubin was altered by the addition of chloroform. In total, the present study suggests a dynamic, unusually flexible high affinity binding site for bilirubin on human serum albumin.
Assuntos
Bilirrubina/sangue , Bilirrubina/química , Albumina Sérica/química , Albumina Sérica/metabolismo , Substituição de Aminoácidos , Sítios de Ligação , Cristalografia por Raios X , Humanos , Cinética , Modelos Químicos , Modelos Moleculares , Conformação Molecular , Mutagênese Sítio-Dirigida , Pichia , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Espectrometria de Fluorescência , TermodinâmicaRESUMO
BACKGROUND: In a previous study, we found that the amino acid substitution R218H in human serum albumin (HSA) was the cause of familial dysalbuminemic hyperthyroxinemia (FDH) in several Caucasian patients. Subsequently the substitution R218P was shown to be the cause of FDH in several members of a Japanese family. This study attempts to resolve discrepancies in the only other study of R218P HSA and identifies two new Japanese R218P FDH patients unrelated to those described previously. METHODS AND RESULTS: Recombinant R218H, R218P, and wild-type HSA were synthesized in yeast, and the affinities of these HSA species for l- and d-thyroxine were determined using fluorescence spectroscopy. The dissociation constants for the binding of wild-type, R218P, and R218H HSA to l-thyroxine were 1.44 x 10(-6), 2.64 x 10(-7), and 2.49 x 10(-7) mol/L, respectively. The circular dichroism spectra of thyroxine bound to R218H and R218P HSA were markedly different, indicating that the structure of the thyroxine/HSA complex is different for either protein. CONCLUSIONS: The K(d) values for l-thyroxine bound to R218P and R218H HSA determined in this study were similar. The extremely high serum total-thyroxine concentrations reported previously for R218P FDH patients (10-fold higher than those reported for R218H FDH patients) are not consistent with the K(d) values determined in this study. Possible explanations for these discrepancies are discussed.
Assuntos
Hipertireoxinemia/genética , Albumina Sérica/genética , Substituição de Aminoácidos , Dicroísmo Circular , Humanos , Hipertireoxinemia/sangue , Pichia/metabolismo , Ligação Proteica , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Albumina Sérica/biossíntese , Albumina Sérica/deficiência , Albumina Sérica/metabolismo , Espectrometria de Fluorescência , Tiroxina/sangueRESUMO
Site-directed mutagenesis and a yeast expression system were used to synthesize a human serum albumin (HSA) fragment (amino acids 1-297). The HSA fragment (half HSA) was evaluated with a number of biophysical techniques and found to be similar to the corresponding region in wild-type HSA. Specifically, the circular dichroism spectra of half HSA and wild-type HSA were superimposable, indicating that the highly alpha-helical secondary structure of wild-type HSA is preserved in half HSA. Additionally, half HSA was partially reactive with a polyclonal antibody against authentic HSA. Half HSA, which contains subdomain IIA, had an affinity for thyroxine and several thyroxine analogs, similar to that observed previously for wild-type HSA. This study suggests that the production of recombinant HSA fragments will be useful for the study of HSA ligand interactions.
Assuntos
Albumina Sérica/genética , Fenômenos Biofísicos , Biofísica , Dicroísmo Circular , Espectroscopia de Ressonância de Spin Eletrônica , Expressão Gênica , Humanos , Cinética , Ligantes , Mutagênese Sítio-Dirigida , Fragmentos de Peptídeos/biossíntese , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/genética , Pichia/genética , Ligação Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Albumina Sérica/biossíntese , Albumina Sérica/química , Tiroxina/análogos & derivados , Tiroxina/metabolismoRESUMO
We have used the electron spin resonance (ESR) site-directed spin-labeling (SDSL) technique to examine the guanidine hydrochloride (Gdn-HCl) induced denaturation of several sites along a transmembrane beta-strand located in the ferric enterobactin receptor, FepA. In addition, we have continued the characterization of the beta-strand previously identified by our group (Klug CS et al., 1997, Biochemistry 36:13027-13033) to extend from the periplasm to the extracellular surface loop in FepA, an integral membrane protein containing a beta-barrel motif comprised of a series of antiparallel beta-strands that is responsible for transport of the iron chelate, ferric enterobactin (FeEnt), across the outer membrane of Escherichia coli and many related enteric bacteria. We have previously shown that a large surface loop in FepA containing the FeEnt binding site denatures independently of the beta-barrel domain (Klug CS et al., 1995, Biochemistry 34:14230-14236). The SDSL approach allows examination of the unfolding at individual residues independent of the global unfolding of the protein. This work shows that sites along the beta-strand that are exposed to the aqueous lumen of the channel denature more rapidly and with higher cooperativity than the surface loop, while sites on the hydrophobic side of the beta-strand undergo a limited degree of noncooperative unfolding and do not fully denature even at high (e.g., 4 M) Gdn-HCl concentrations. We conclude that, in a transmembrane beta-strand, the local environment of a given residue plays a significant role in the loss of structure at each site.
Assuntos
Proteínas da Membrana Bacteriana Externa , Proteínas de Transporte/química , Membrana Celular/química , Guanidina/farmacologia , Desnaturação Proteica , Espectroscopia de Ressonância de Spin Eletrônica , Escherichia coli/genética , Mutagênese Sítio-Dirigida , Dobramento de Proteína , Receptores de Superfície Celular , Marcadores de Spin , TermodinâmicaRESUMO
A mutant of the ferric enterobactin receptor, FepA, containing a valine to cysteine (V338C) substitution was made and the purified protein selectively modified with a sulfhydryl-specific nitroxide spin label. In reconstituted liposomes, interaction of the attached spin label with a combination of water-soluble and lipid-soluble relaxation agents indicated that the V338C site was located in the polar headgroup region of the membrane, approximately 1.5-4.5 A above the phosphate groups of the lipids. Binding of the ligand, ferric enterobactin (FeEnt), to the purified spin-labeled protein produced a significant decrease in both the rotational freedom and accessibility of the nitroxide, indicating the formation of new structural contacts between the spin label and either the protein or the bound ligand. Electron spin-echo (ESE) measurements of the nitroxide phase-memory relaxation rate in the presence and absence of bound ligand showed substantial dipolar coupling between the Fe3+ of FeEnt and the spin label and provided an iron-nitroxide distance estimate in the range of 20-30 A. We conclude that the ligand-induced changes in spin label motion and accessibility are due to new tertiary contacts with the protein and not to direct contact with the ligand. These studies suggest that V338C may occupy a hinge region connecting the ligand binding surface loop to the beta-barrel and provide the strongest evidence to date of an in vitro ligand-induced conformational change in FepA.
Assuntos
Proteínas da Membrana Bacteriana Externa , Proteínas de Transporte/química , Enterobactina/metabolismo , Conformação Proteica , Receptores de Superfície Celular , Proteínas de Transporte/genética , Cisteína/genética , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Ligantes , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Lipossomos , Mutagênese Sítio-Dirigida , Marcadores de Spin , Valina/genéticaRESUMO
Electron paramagnetic resonance (EPR) site-directed spin-labeling (SDSL) has been used to characterize a proposed transmembrane beta-strand of the Escherichia coli ferric enterobactin receptor, FepA. Each of nine consecutive residues was mutated to cysteine and subsequently labeled with the sulfhydryl-specific spin-label methanethiosulfonate (MTSL) and the purified protein reconstituted into liposomes. Continuous wave (CW) power saturation methods were used to determine exposure of the nitroxide side chains to a series of paramagnetic relaxation agents, including nickel acetylacetonate (NiAA), nickel ethylenediaminediacetate (NiEDDA), chromium oxalate (CROX), and molecular oxygen. The spin-label attached to Q245C, L247C, L249C, A251C, and Y253C had higher collision frequencies with molecular oxygen than with polar relaxation agents, indicating that these sites are exposed to the hydrophobic phase of the lipid bilayer. MTSL bound to residues S246C, E248C, E250C, and G252C had higher collision rates with the polar agents than with oxygen, suggesting that these sites are exposed to the aqueous channel. The alternating periodicity observed with the polar relaxation agents, NiAA and NiEDDA, and in opposite phase with oxygen, is consistent with beta-sheet structure. Depth measurements, based on the reciprocal concentration gradients of NiEDDA and O2 across the bilayer and calibrated for our system with phosphatidylcholine spin-labels, indicated that L249C was nearest the center of the bilayer and that Q245C and Y253C were located just below the bilayer surface in opposite leaflets of the membrane. Thus, we conclude that this approach, through mapping of individual residues, has the capability of defining beta-sheet secondary structure.
Assuntos
Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Estrutura Secundária de Proteína , Sequência de Aminoácidos , Substituição de Aminoácidos , Proteínas da Membrana Bacteriana Externa/química , Proteínas da Membrana Bacteriana Externa/metabolismo , Sítios de Ligação , Proteínas de Transporte/isolamento & purificação , Colicinas/farmacologia , Cisteína , Ácido Edético/análogos & derivados , Espectroscopia de Ressonância de Spin Eletrônica , Enterobactina , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/metabolismo , Hidroxibutiratos , Bicamadas Lipídicas , Lipossomos , Mesilatos , Modelos Estruturais , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Níquel , Oxalatos , Oxigênio , Pentanonas , Receptores de Superfície Celular/química , Receptores de Superfície Celular/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Marcadores de SpinRESUMO
Ligand-gated membrane channels selectively facilitate the entry of iron into prokaryotic cells. The essential role of iron in metabolism makes its acquisition a determinant of bacterial pathogenesis and a target for therapeutic strategies. In Gram-negative bacteria, TonB-dependent outer membrane proteins form energized, gated pores that bind iron chelates (siderophores) and internalize them. The time-resolved operation of the Escherichia coli ferric enterobactin receptor FepA was observed in vivo with electron spin resonance spectroscopy by monitoring the mobility of covalently bound nitroxide spin labels. A ligand-binding surface loop of FepA, which normally closes its transmembrane channel, exhibited energy-dependent structural changes during iron and toxin (colicin) transport. These changes were not merely associated with ligand binding, but occurred during ligand uptake through the outer membrane bilayer. The results demonstrate by a physical method that gated-porin channels open and close during membrane transport in vivo.
Assuntos
Proteínas da Membrana Bacteriana Externa , Proteínas de Transporte/metabolismo , Enterobactina/metabolismo , Proteínas de Escherichia coli , Escherichia coli/metabolismo , Ativação do Canal Iônico , Porinas/metabolismo , Receptores de Superfície Celular/metabolismo , Proteínas de Bactérias/metabolismo , Transporte Biológico/efeitos dos fármacos , Proteínas de Transporte/genética , Colicinas/farmacologia , Óxidos N-Cíclicos , Cisteína/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Enterobactina/farmacologia , Compostos Férricos/metabolismo , Compostos Férricos/farmacologia , Indicadores e Reagentes , Ligantes , Proteínas de Membrana/metabolismo , Mesilatos , Conformação Proteica , Marcadores de SpinRESUMO
FepA is an integral outer membrane protein that is the specific receptor for the siderophore, ferric enterobactin, and is thus primarily responsible for iron uptake in many Gram-negative bacteria. A site-specific mutant of FepA, containing a single introduced cysteine in the ligand-binding domain, was spin labeled and used to examine the denaturant-induced unfolding of this receptor with guanidine hydrochloride (Gdn-HCl) and urea. Electron spin resonance (ESR) spectra showed conversion of the spin label from a motionally-restricted, immobilized environment to a freely-accessible, rotationally-mobile state upon denaturation. Unfolding was also followed by nondenaturing polyacrylamide gel electrophoresis (PAGE), which is sensitive to loss of the putative transmembrane beta-structure, and displayed a similar concentration dependence. Unfolding occurred over relatively narrow ranges of denaturant concentration, indicating a high degree of cooperativity. Unfolding was fully reversible under the conditions employed. Rapid, spontaneous refolding occurred in the presence of Triton X-100 and did not require exogenous lipids. Refolding could be induced by either dialysis, dilution to low denaturant concentration, or ethanol precipitation. At ambient temperature the free energy of unfolding extrapolated to zero denaturant concentration (delta GU zero) was 6.24 +/- 0.63 kcal/mol. Values of delta GU zero obtained with Gdn-HCl and urea were in good agreement, as were values obtained from linear extrapolation and nonlinear regression fitting to a two-state equilibrium. This is the first report of a quantitative evaluation of the free energy of unfolding for an integral membrane protein.
Assuntos
Proteínas da Membrana Bacteriana Externa/química , Proteínas de Transporte/química , Enterobactina/metabolismo , Ferro/química , Dobramento de Proteína , Receptores de Superfície Celular , Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Transporte/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Eletroforese em Gel de Poliacrilamida , Ligantes , Ligação Proteica , Desnaturação Proteica , Marcadores de Spin , TermodinâmicaRESUMO
In this study, we have spin-labeled the lysine and cysteine residues of low-density lipoprotein (LDL) using N-4-(2,2,6,6-tetramethylpiperidinyl-1-oxyl-4-yl) maleimide (MAL-6) and succinimidyl-2,2,5,5-tetramethyl-3-pyrroline-1-oxyl-3-carboxylate (SSL), respectively. The electron spin resonance (ESR) spectrum of SSL bound to LDL indicated that the nitroxide moiety was relatively mobile. In contrast, the ESR spectrum of MAL-6 bound to LDL showed that the nitroxide moiety was rotationally restricted. Using the continuous-wave power saturation technique in the presence of hydrophobic and hydrophilic paramagnetic relaxing agents, we have determined that (i) approximately 60-70% of lysine-bound SSL is exposed to the aqueous phase, (ii) approximately 30-40% of SSL-LDL is buried in a hydrophobic region, and (iii) MAL-6 bound to LDL is localized predominantly in the hydrophobic region. During Cu(2+)-initiated oxidation of spin-labeled LDL, nitroxide labels located in a hydrophobic environment were predominantly degraded. Nitroxide destruction was inhibited by butylated hydroxytoluene, indicating the role of lipid peroxidation in this process. ESR data also showed that Cu2+ binding to lysine is essential for LDL oxidation. The spin label methodology may be useful for the investigation of site-specific radical reactions in LDL.
Assuntos
Lipoproteínas LDL/química , Apolipoproteínas B/química , Sítios de Ligação , Óxidos N-Cíclicos , Cisteína/química , Espectroscopia de Ressonância de Spin Eletrônica , Radicais Livres , Humanos , Técnicas In Vitro , Peroxidação de Lipídeos , Lisina/química , Micro-Ondas , Estrutura Molecular , Oxirredução , Marcadores de SpinRESUMO
The ferric enterobactin receptor, FepA, is a TonB-dependent gated porin that transports the siderophore ferric enterobactin across the outer membrane of gram-negative bacteria. We have created two site-directed mutants of Escherichia coli FepA, in both cases introducing a cysteine residue into the putative ligand-binding domain. The introduced cysteines were then modified with nitroxide spin labels for structural and dynamic studies using electron spin resonance (ESR) spectroscopy. The mutants were fully functional, as indicated by their ability to grow under iron-limiting conditions, their uptake of [59Fe]enterobactin, and their sensitivity to colicin B. Labeling of the mutant FepA receptors proceeded easily upon incubation with sulfhydryl-specific spin labels, e.g. MTSL, (1-oxy-2,2,5,5-tetramethylpyrrolidin-3-yl)methyl methanethiosulfonate. In contrast, spin labeling of the two native cysteines (Cys486 and Cys493) within wild-type FepA occurred only after treatment with a thiol reducing agent and partial denaturation in urea, suggesting that the native cysteines are disulfide-linked. ESR spectra showed a high degree of motional restriction for all three sites. Continuous wave (CW) saturation studies indicated that one of the mutationally introduced sites (Cys280) was surface-localized as evidenced by its exposure to the aqueous paramagnetic relaxation agent chromium oxalate and its low accessibility to O2. The other (Cys310) apparently occupies a site near the membrane/aqueous interface. The native cysteines occupy a site tightly packed within the protein structure with low accessibility to both CROX and O2. A shift in both conventional and saturation-transfer ESR spectra of MTSL-labeled E280C and E310C (but not MTSL-labeled wild type) FepA was observed upon addition of ferric enterobactin. The ESR spectral shift was dependent on ferric enterobactin concentration and did not occur with siderophores not recognized by FepA. Ferric enterobactin binding did not alter the CW saturation properties of MTSL bound to these sites, but did influence their accessibility to O2. These results provide consistent evidence for a ligand-specific conformational change in the surface peptides of FepA upon the binding of ferric enterobactin.
Assuntos
Proteínas da Membrana Bacteriana Externa , Proteínas de Transporte/metabolismo , Escherichia coli/metabolismo , Receptores de Superfície Celular , Sequência de Bases , Proteínas de Transporte/genética , Cisteína/metabolismo , DNA Complementar , Espectroscopia de Ressonância de Spin Eletrônica , Escherichia coli/genética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Plasmídeos , Conformação Proteica , Ensaio Radioligante , Marcadores de SpinRESUMO
Salicylate and several structurally analogous compounds enhance merocyanine 540 (MC540)-photosensitized killing of leukemia cells (M. A. Anderson, B. Kalyanaraman, and J. B. Feix, Cancer Res., 53: 806-809, 1993). In this work, we show that salicylic acid enhances the binding of MC540 prior to illumination, as well as the light-stimulated uptake of MC540 by target L1210 murine and K562 human leukemia cells. Acetylsalicylic acid, 2,3- and 2,5-dihydroxybenzoic acids, and sodium benzoate also enhance MC540 uptake. The irradiation dose responses for loss of cell survival and enhanced MC540 uptake are well correlated, both being shifted to earlier time points in the presence of salicylate. Salicylic acid also enhanced photodynamic cell killing of A549 lung carcinoma and NIH:OVCAR-3 ovarian carcinoma cells, two cell types which are relatively resistant to MC540-mediated photosensitization. Cellular uptake of the anionic, potential-sensitive oxonol dye, bis-(1,3-dibutylbarbituric acid)-trimethine oxonol, is also increased by salicylate in a dose-dependent fashion. In contrast, cellular uptake of the cationic cyanine dye, 3,3'-dihexyloxacarbocyanine, is unaffected by salicylate. These studies suggest that increased uptake of MC540 is the basis of salicylate enhancement and that changes in plasma membrane potentials may play a mechanistic role in the potentiation of MC540 binding and cell killing.
Assuntos
Neoplasias/tratamento farmacológico , Fotoquimioterapia , Fármacos Fotossensibilizantes/uso terapêutico , Pirimidinonas/uso terapêutico , Salicilatos/uso terapêutico , Animais , Benzoatos/uso terapêutico , Ácido Benzoico , Sobrevivência Celular/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Sinergismo Farmacológico , Fluorescência , Humanos , Leucemia/tratamento farmacológico , Leucemia/metabolismo , Leucemia L1210/tratamento farmacológico , Leucemia L1210/metabolismo , Camundongos , Neoplasias/metabolismo , Fármacos Fotossensibilizantes/farmacocinética , Pirimidinonas/farmacocinética , Ácido SalicílicoRESUMO
The binding and state of aggregation of cecropin in large unilamellar vesicles of different surface potential and varying acyl chain length were examined using a Cys-33 spin-labeled derivative of cecropin AD (CAD). Association isotherms of the peptide were measured for vesicles of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) containing 5, 15, and 30 mol % 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG). The isotherms display a concentration-dependent positive cooperativity indicating the possible formation of cecropin aggregates in the lipid phase. The critical aqueous concentration for aggregation was dependent on the fraction of POPG, suggesting the involvement of acidic lipids in the formation and stabilization of the putative aggregate. Our data also indicate that cooperativity depends on the state of side-chain ionization of an acidic residue that titrates between pH 7 and 4.4. The binding of spin-labeled Cys-33 CAD was found to be influenced by the acyl chain length of the host lipid. The association isotherm of the peptide for dilaureoyl-sn-glycero-3-phosphatidylcholine vesicles containing 30 mol % dilauroyl-sn-glycero-3-phosphatidylglycerol (DLPG) differed significantly from that in POPC/POPG and could be interpreted in terms of a monomer-monomer partitioning between the aqueous and lipid phases. ESR line-shape analysis was consistent with peptide aggregation in dioleoyl-sn-glycero-3-phosphatidylglycerol vesicles but not in DLPG vesicles.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Anti-Infecciosos/metabolismo , Ácidos Graxos/química , Hormônios de Inseto/metabolismo , Proteínas de Insetos , Bicamadas Lipídicas , Fosfatidilcolinas/metabolismo , Fosfatidilgliceróis/metabolismo , Sequência de Aminoácidos , Anti-Infecciosos/química , Sítios de Ligação , Eletroquímica , Espectroscopia de Ressonância de Spin Eletrônica , Concentração de Íons de Hidrogênio , Hormônios de Inseto/química , Dados de Sequência Molecular , Marcadores de Spin , TemperaturaRESUMO
A spin-labeled derivative of the ion channel peptide cecropin AD (Fink et al., 1988) was synthesized and used to investigate its aggregation state in water and in the presence of a helix-promoting solvent. A cysteine was introduced at position 33 and spin-labeled using the methanethiosulfonate spin label. In low ionic strength aqueous solution, the peptide is monomeric, and the ESR spectrum indicates a high degree of segmental flexibility at the nitroxide attachment point, consistent with a predominantly random coil conformation. Upon addition of 5-10% (v/v) hexafluoro-2-propanol (HFP), the peptide is induced to aggregate as evidenced by significant motional restriction of the spin label and spin-spin broadening of the ESR lines. At higher concentrations of HFP, the peptide reverts to a monomeric state but retains its folded conformation. Our data suggest that between 5 and 10% HFP the peptide undergoes two structural transitions. The first transition starts at 5% and is very cooperative. Its dependence on ionic strength, temperature, and pH indicates that it involves the interconversion between a random coil and an ordered state stabilized by interpeptide electrostatic and hydrophobic interactions. The second transition, which occurs at 11% v/v HFP, is between the self-associated form and an ordered monomeric form. The analysis of our experimental results demonstrates aggregate formation at 5-10% HFP. This may be relevant to the mechanism of channel formation by cecropins in membranes.
Assuntos
Hormônios de Inseto/química , Proteínas de Insetos , Sequência de Aminoácidos , Cromatografia Líquida de Alta Pressão , Óxidos N-Cíclicos , Cisteína , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Indicadores e Reagentes , Hormônios de Inseto/síntese química , Canais Iônicos/química , Dados de Sequência Molecular , Propanóis , Ligação Proteica , Conformação Proteica , Estrutura Secundária de Proteína , Soluções , Marcadores de Spin , Termodinâmica , UreiaRESUMO
FepA is an Escherichia coli outer membrane receptor protein for the siderophore ferric enterobactin. Prior studies conducted in vivo suggested that FepA and other TonB-dependent outer membrane proteins transport ligands by a gated-channel mechanism. To corroborate and extend these findings we have determined the permeability properties of the FepA channel in vitro, by measuring the diffusion rates of hydrophilic nonelectrolytes through the FepA channel in liposome swelling experiments. Like porins, the FepA deletion mutant delta RV showed a size-dependent permeability to oligosaccharides, indicating that it forms a nonspecific, hydrophilic pore. Unlike OmpF and other E. coli porins, however, delta RV proteoliposomes transported stachyose (666 Da) and ferrichrome (740 Da). These data, and other uptake results with a series of maltodextrins of increasing size, confirm the existence of a channel domain within FepA that is considerably larger than OmpF-type pores. These results represent a reconstitution of the channel function of a TonB-dependent receptor protein and establish that FepA contains the largest channel that has been characterized in the E. coli outer membrane.
Assuntos
Proteínas de Transporte/química , Escherichia coli/fisiologia , Ativação do Canal Iônico , Canais Iônicos/fisiologia , Porinas/química , Receptores de Superfície Celular/fisiologia , Proteínas da Membrana Bacteriana Externa/química , Proteínas de Transporte/fisiologia , Deleção de Genes , Técnicas In Vitro , LipossomosRESUMO
Merocyanine 540 (MC540) is a photosensitizing dye of potential use in the purging of cancer cells from autologous bone marrow explants. Treatment of marrow with MC540, followed by illumination with visible light, selectively kills neoplastic cells while sparing a sufficient number of stem cells to allow marrow engraftment. The photodynamic action of MC540 is thought to be mediated by reactive oxygen species, particularly singlet oxygen. We have previously shown that salicylic acid (SA) scavenges MC540-generated singlet oxygen. In this work, we sought to abrogate MC540-mediated cell killing of murine L1210 and human K562 leukemia cells with salicylate. Paradoxically, the presence of salicylate during illumination in the presence of MC540 appreciably enhanced cell killing. Enhancement was dependent on salicylate concentration in the range 0.1 to 10 mM, with 1.0 mM SA potentiating the MC540-mediated reduction in survival of L1210 and K562 cells by factors of 2.7 and 1.9, respectively. Neither preincubation with SA followed by washing prior to illumination nor addition of SA following illumination altered MC540-mediated cell killing, indicating that potentiation was dependent on the presence of SA during illumination. Illumination in the presence of salicylate alone did not diminish cell viability. In addition to SA, a number of structurally related compounds including dihydroxybenzoic acids, aspirin, and sodium benzoate also enhanced MC540-mediated cell killing. Potentiation of leukemic cell killing by salicylate could provide a basis for enhancing the clinical efficacy of MC540-mediated phototherapy.
Assuntos
Gentisatos , Leucemia/tratamento farmacológico , Fotoquimioterapia , Fármacos Fotossensibilizantes/uso terapêutico , Pirimidinonas/uso terapêutico , Salicilatos/uso terapêutico , Animais , Sobrevivência Celular/efeitos dos fármacos , Sinergismo Farmacológico , Humanos , Hidróxidos/metabolismo , Hidroxibenzoatos/uso terapêutico , Radical Hidroxila , Leucemia L1210/tratamento farmacológico , Oxigênio/metabolismo , Ácido SalicílicoRESUMO
The ferric enterobactin receptor (FepA) is a high-affinity ligand-specific transport protein in the outer membrane of Gram-negative bacteria. Deletion of the cell-surface ligand-binding peptides of FepA generated mutant proteins that were incapable of high-affinity uptake but that instead formed nonspecific, passive channels in the outer membrane. Unlike native FepA, these pores acted independently of the accessory protein TonB, which suggests that FepA is a gated porin and that TonB acts as its gatekeeper by facilitating the entry of ligands into the FepA channel. The sequence homology among TonB-dependent proteins suggests that all ligand-specific outer membrane receptors may function by this gated-porin mechanism.
Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Transporte/metabolismo , Ativação do Canal Iônico , Receptores de Superfície Celular , Sequência de Aminoácidos , Antígenos de Bactérias/química , Antígenos de Superfície/química , Proteínas da Membrana Bacteriana Externa/química , Proteínas da Membrana Bacteriana Externa/imunologia , Proteínas de Transporte/imunologia , Análise Mutacional de DNA , Enterobactina/metabolismo , Escherichia coli/genética , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Relação Estrutura-AtividadeRESUMO
The purpose of this study was to assess the mechanism of merocyanine 540 (MC540) photobleaching in a liposomal system. Broad based visible irradiation of MC540 in unilamellar dilauroylphosphatidylcholine (DLPC) vesicles resulted in dye bleaching that was strictly O2 dependent. The rate of self-sensitized photobleaching was enhanced in D2O and inhibited by both azide and histidine, consistent with 1O2 intermediacy (Type II chemistry). Supportive evidence for this mechanism was obtained by using a Type II sensitizer, aluminum phthalocyanine tetrasulfonate (AlPcS lambda max = 678 nm). Irradiation of AlPcS and MC540 in DLPC with lambda greater than 630 nm (absorbed only by AlPcS) light resulted in rapid bleaching of MC540, which was stimulated by D2O and inhibited by azide. A rate constant of 10(7) M-1 s-1 was determined for the chemical quenching of 1O2 by MC540. The rate constant for physical quenching of 1O2 by MC540 was estimated to be ca 10(9) M-1 s-1.
Assuntos
Oxigênio , Pirimidinonas/efeitos da radiação , Radiossensibilizantes/efeitos da radiação , Lipossomos , Estrutura Molecular , Fosfatidilcolinas , Fotoquímica , Oxigênio SingleteRESUMO
A protonated, charged free radical of methylphenazine methosulfate (PMS) was generated at carbon electrodes in a buffered aqueous medium. This radical diffused from the aqueous phase into nonpolar organic solvents, where it was stable for extended periods. The electron spin resonance (ESR) spectrum of the free radical species in the nonpolar solvent was significantly different from that of the aqueous species. This difference was attributed to the loss of electric charge through deprotonation at the solution interface, followed by solvation of the uncharged species in the organic phase. ESR spectra are presented for PMS free radicals in polar and nonpolar liquid phases, along with electrochemical results and conclusions regarding the mechanisms of movement and toxicity of phenazyl free radicals in biological systems.
Assuntos
Metilfenazônio Metossulfato/química , Eletroquímica , Radicais Livres , Metilfenazônio Metossulfato/toxicidade , Solventes , ÁguaRESUMO
Action spectra of the antileukemic and antiviral activities of merocyanine 540 (MC540) were determined using L1210 leukemia cells and human Herpes simplex virus type 1. The major peak of both action spectra aligned closely with the absorption spectrum of membrane-bound dye monomer, and by implication, the action spectrum of 1O2 generation. These results are compatible with the notion that the antileukemic and antiviral activities of MC540 are primarily attributable to membrane-bound monomer and at least in part mediated by 1O2.
Assuntos
Corantes Fluorescentes/efeitos da radiação , Pirimidinonas/efeitos da radiação , Simplexvirus/efeitos da radiação , Animais , Herpes Simples , Humanos , Leucemia , Membranas/efeitos da radiação , Camundongos , Espectrofotometria , Células Tumorais CultivadasRESUMO
This paper examines the relationship between lipid composition, plasma membrane fluidity, expression of dye binding sites, and susceptibility to merocyanine 540 (MC540)-sensitized irradiation in L1210 leukemia cells. Reducing the cells' cholesterol content by exchange diffusion with phosphatidylcholine liposomes or by inhibiting its biosynthesis with 25-hydroxycholesterol enhanced plasma membrane fluidity, the expression of dye binding sites, and the cells' susceptibility to MC540-sensitized irradiation. Conversely, if the cholesterol content was enhanced by exchange diffusion with cholesterol:phosphatidylcholine liposomes, the cells' susceptibility to MC540-sensitized irradiation was decreased. However, contrary to expectations, dye-binding was slightly enhanced and plasma membrane fluidity remained unchanged. Growing the cells in fatty acid-supplemented medium had profound effects on their lipid composition. Cells enriched in polyunsaturated fatty acids had more fluid plasma membranes. However, dye-binding was not significantly affected and photosensitivity was slightly reduced. These results suggest that cholesterol is one, but probably not the only, determinant of the expression of cellular dye binding sites and, consequently, the cell's susceptibility to MC540-sensitized irradiation. By contrast, plasma membrane fluidity does not appear to play a major role in the regulation of dye-binding site expression.