Plant hormones mediate the interaction between oak acorn germination and rodent hoarding behaviour.

The interaction between animals and plants for seed dispersal and predation has received much attention; however, the underlying physiological mechanisms driving the responses of both seeds and animals remain unclear. We conducted a series of behaviour and physiology experiments to examine the role of plant hormones in regulating seed germination and rodent hoarding behaviour in the Quercus variabilis and Leopoldamys edwardsi systems. We found that acorns that were partially consumed by rodents had increased gibberellin (GA) levels and shortened germination time. Rodents preferred scatter-hoarded abscisic acid (ABA)-treated and intact acorns but consumed germinated and GA-treated acorns; such treatment differences disappeared for inactivated acorns by boiling water. Moreover, we found that seven potential compounds may be linked to seed germination and rodent hoarding behaviour. Our results indicate that acorns of oak showed rapid germination when facing predation risk, while rodents could identify the germination status of seeds for hoarding; GA and ABA may play an important role in regulating seed germination of oak and hoarding behaviour of rodents.

Gut microbiota is associated with spatial memory and seed-hoarding behavior of South China field mice (Apodemus draco).

Background: Scatter-hoarding animals store food in multiple locations within their home range and rely on spatial memory for subsequent localization and retrieval. The relationship between memory and scatter-hoarding behavior has been widely demonstrated, but the association of gut microbiota with spatial memory and seed-hoarding behavior of animals remains unclear. Methods: In this study, by using enclosure behavior tests, memory tests including an object location test (OLT) and a novel object recognition test (NORT), and fecal microbiota transplantation (FMT) experiment, we evaluated the role of gut microbiota in affecting the memory and seed-hoarding behavior of rodents. According to their scatter-hoarding intensity, South China field mice (Apodemus draco) were divided into scatter-hoarding group (SG) and non-scatter-hoarding group (NG). Results: We found that the SG performed better than the NG in the NORT. FMT from SG donor mice altered the NG recipient mice's gut microbiota structure. Further tests demonstrated FMT from SG donor mice increased memory of NG recipient mice in laboratory tests and seed larder hoarding intensity of NG recipient mice in enclosures. Conclusion: Our results suggest gut microbiota could modulate the memory and seed-hoarding behavior of animals.