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1.
Appl Opt ; 63(6): 1590-1599, 2024 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-38437373

RESUMO

The polarization imaging technique leverages the disparity between target and background polarization information to mitigate the impact of backward scattered light, thereby enhancing image quality. However, the imaging model of this method exhibits limitations in extracting inter-image features, resulting in less-than-optimal outcomes in turbid underwater environments. In recent years, machine learning methodologies, particularly neural networks, have gained traction. These networks, renowned for their superior fitting capabilities, can effectively extract information from multiple images. The incorporation of an attention mechanism significantly augments the capacity of neural networks to extract inter-image correlation attributes, thereby mitigating the constraints of polarization imaging methods to a certain degree. To enhance the efficacy of polarization imaging in complex underwater environments, this paper introduces a super-resolution network with an integrated attention mechanism, termed as SRGAN-DP. This network is a fusion of an enhanced SRGAN network and the high-performance deep pyramidal split attention (DPSA) module, also proposed in this paper. SRGAN-DP is employed to perform high-resolution reconstruction of the underwater polarimetric image dataset, constructed specifically for this study. A comparative analysis with existing algorithms demonstrates that our proposed algorithm not only produces superior images but also exhibits robust performance in real-world environments.

2.
Anim Reprod Sci ; 258: 107359, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-37832280

RESUMO

L-proline has been reported to be useful in semen cryopreservation. However, its use has rarely been reported in the freezing of boar semen. The objective of this study was to evaluate the effects of different concentrations of L-proline (0, 10, 30, 50, and 90 mM) on the quality of boar semen after freezing and thawing. Semen samples from boars (n = 6) were frozen using freezing extenders with added concentrations of L-proline. Total sperm motility, progressive motility, survival time at 37 °C, acrosome integrity, mitochondrial activity, DNA integrity, the content of the lipid peroxidation product malondialdehyde (MDA), total antioxidant capacity (T-AOC) and, expression levels of apoptosis protein (cleaved caspase 3 and Bax) were evaluated after thawing. The results showed that total sperm viability (73.96% vs. 63.58%) and progressive motility (56.88% vs. 47.26%) after thawing were significantly higher in the 10 mM L-proline treatment group than in the control group. The survival time at 37 °C and the total motility of sperm in the 10 mM group within one hour after thawing were significantly higher than in the control group. Acrosome integrity and mitochondrial activity of sperm in the 10 mM group were significantly higher than those in the control, 50 mM, and 90 mM groups. The DNA integrity rate in the 10 mM group was significantly higher than in the control group. The L-proline treatment did not affect sperm MDA content or T-AOC. The expression levels of apoptosis protein (cleaved caspase 3 and Bax) in the 10 mM L-proline supplemented group were lower than those in the control group. In conclusion, the freezing extender containing 10 mM L-proline improved semen quality after freezing and thawing and thus would be a useful reagent for boar semen cryopreservation.


Assuntos
Preservação do Sêmen , Sêmen , Masculino , Suínos , Animais , Caspase 3/farmacologia , Análise do Sêmen/veterinária , Proteína X Associada a bcl-2 , Motilidade dos Espermatozoides , Espermatozoides , Criopreservação/veterinária , Criopreservação/métodos , Preservação do Sêmen/veterinária , Preservação do Sêmen/métodos , DNA , Crioprotetores/farmacologia
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