Assuntos
Anormalidades Congênitas/genética , Meato Acústico Externo/anormalidades , Queratinas Específicas do Cabelo/genética , Queratinas Tipo II/genética , Monilétrix/genética , Adulto , Povo Asiático/genética , China , Anormalidades Congênitas/patologia , Análise Mutacional de DNA , Orelha/anormalidades , Orelha/patologia , Feminino , Humanos , Masculino , Monilétrix/patologia , Mutação , LinhagemRESUMO
AIM: To investigate the expression of CD1a and CD207 in condyloma acuminatum (CA) epidermis and to understand its significance. METHODS: The mRNA expression of CD1a and CD207 in six CA epidermal lesions and in six normal controls were detected using oligonucleotide microarrys and confirmed by semi-quantitative RT-PCR, and the protein level of CD1a and CD207 in six CA epidermal lesions and in six normal controls were measured by Western blot. RESULTS: With microarrys, the mRNA expression of CD1a and CD207 were detected markedly down-regulated in six CA epidermal lesions as compared with that in six normal controls. Moreover, the down-regulation of CD1a and CD207 was verified by semi-quantitative RT-PCR. Western blot analysis showed that the protein expression of CD1a and CD207 in six CA epidermal lesions were significantly lower than that in normal controls. CONCLUSION: The expression of CD1a and CD207 is markedly down-regulated in CA epidermis compared with that in normal epidermis, and the results may suggest that the number of LC in CA epidermis is decreased and the function is impaired.
Assuntos
Antígenos CD1/genética , Antígenos CD/genética , Condiloma Acuminado/genética , Regulação Neoplásica da Expressão Gênica , Lectinas Tipo C/genética , Lectinas de Ligação a Manose/genética , Adolescente , Adulto , Antígenos CD/metabolismo , Antígenos CD1/metabolismo , Western Blotting , Epiderme/metabolismo , Epiderme/patologia , Humanos , Lectinas Tipo C/metabolismo , Masculino , Lectinas de Ligação a Manose/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Adulto JovemRESUMO
BACKGROUND: There are few studies on the abnormal morphology of Langerhans cells (LCs) in condyloma acuminatum (CA) lesions and the essence of the abnormal morphology of LCs in CA lesions is still not well elucidated. The aim of this study was to further investigate the morphological features of LCs in CA lesions. METHODS: CD1a(+) LCs in 13 CA lesions and in 13 normal controls were labeled using immunohistochemistry and examined by light microscopy. Ultrastructural investigation on LCs in six CA lesions and in six normal controls was performed by electron microscopy. RESULTS: Compared with those in normal controls, most CD1a(+) LCs in CA lesions exhibited dysplastic dendrities and abnormal distribution. The number of CD1a(+) LCs in CA lesions (26.31 +/- 18.84) was statistically lower (p < 0.001) than that in normal controls (72.00 +/- 27.40). Electron microscopy showed that the number of Birbeck granules within lesional LCs (4.00 +/- 2.94) was significantly decreased (p < 0.001) than that within normal LCs (10.80 +/- 4.78). The ultrastructures of most lesional LCs displayed degenerative changes. CONCLUSIONS: The morphology of most LCs in CA lesions shows degenerative changes, which suggest that these LCs have been functionally impaired.
Assuntos
Condiloma Acuminado/patologia , Prepúcio do Pênis/ultraestrutura , Células de Langerhans/ultraestrutura , Doenças do Pênis/patologia , Adulto , Antígenos CD1/metabolismo , Biomarcadores/metabolismo , Contagem de Células , Condiloma Acuminado/metabolismo , Grânulos Citoplasmáticos/ultraestrutura , Técnica Direta de Fluorescência para Anticorpo , Prepúcio do Pênis/metabolismo , Humanos , Técnicas Imunoenzimáticas , Células de Langerhans/metabolismo , Masculino , Microscopia Eletrônica de Transmissão , Doenças do Pênis/metabolismoRESUMO
OBJECTIVE: To detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum (CA) and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis. METHODS: Gene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting. RESULTS: Microarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis. CONCLUSION: The protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.
Assuntos
Quimiocina CCL20/metabolismo , Condiloma Acuminado/metabolismo , Receptores CXCR4/metabolismo , Adulto , Western Blotting , Quimiocina CCL20/genética , Condiloma Acuminado/genética , Regulação para Baixo , Epiderme/metabolismo , Epiderme/patologia , Regulação da Expressão Gênica , Humanos , Análise de Sequência com Séries de Oligonucleotídeos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores CXCR4/genética , Adulto JovemRESUMO
AIM: To investigate differential gene expression of Langerhans cell-related chemokines in condyloma acuminatum (CA) epidermis and normal epidermis. METHODS: Gene expression of Langerhans cell-related chemokines in three CA epidermal lesions and in three normal controls was screened using Affymetrix oligonucleotide microarrays HG-U 133A 2.0, and part of the above differential gene expression was confirmed by semi-quantitative RT-PCR. RESULTS: With microarrays, seven down-regulated genes of Langerhans cell-related chemokine were detected in three CA epidermal lesions as compared with three normal controls, and the down-regulation of CXCR4 and CCL20 was verified by semi-quantitative RT-PCR. CONCLUSION: Several Langerhans cell-related chemokine genes are found down-regulated in CA epidermis as compared with normal epidermis, and the down-regulation of these genes may contribute to the decreased number and the homing disturbance of LC in CA epidermis.
