RESUMO
The use of pesticides in agricultural sectors is rising due to the growing demand for food in the world, but the presence of pesticide residues in agricultural commodities has become a major health concern for consumers and is associated with problems of food safety. Thus, the present study determined pesticide residues (17 organochlorine, 5 pyrethroid and 3 organophosphate) in 77 fish feed, 112 fish and 135 vegetables samples (total of 324) from the different locations in Bangladesh, using quick easy cheap effective rugged and safe (QuEChERS) extraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. The 77 fish feed samples analyzed with this method appeared to be free of pyrethroid pesticide residues. Organochlorine pesticide residues aldrin (0.03 ± 0.01 mg/kg) and pyrethroid pesticide residues permethrin (0.08 ± 0.01 mg/kg) were detected in fish samples of catla (Catla catla) from Rajshahi Durgapur and bata (Labeo bata) from Satkhira Kaligonj, respectively. Among 135 vegetable samples (country bean, green chili, tomato, eggplants and red amaranth), 27.4% were found positive for organophosphate pesticide residues of which 89.2% exceeded maximum residue limits (MRLs) set by the Codex Alimentarius Commission. The study revealed that few fish (catla and bata) but the majority of vegetable samples tested positive for pesticide residues exceeding MRLs. Finally, the study suggests that an effective management strategy is needed for strict regulation and regular monitoring of pesticides in fish feed, fish and vegetables to make aware the farmers and consumers about the harmful effect of pesticides on human health.
RESUMO
The presence of undesirable heavy metals, pesticide residues, and microbial contaminants in fresh produces is a worldwide public health concern. This study was undertaken to evaluate the residual pesticides (Diazinon, Malathion, Cypermethrin, Dimethoate, Quinalphos, and Chloropyrofos), heavy metal contamination (Pb, Cd, and Cr), and microbiological quality and safety of 4 common raw salad vegetables (RSVs) samples from different local markets in Dhaka. Results showed the presence of heavy metals residues were within the acceptable limits of local and international standards. None of the above-mentioned pesticides were found in tomato and cucumber samples but presence of Dimethoate was noticed in 13 coriander samples (12.94-158.3 µg/kg) and 7 lettuce samples (9.6-74.8 µg/kg) exceeding the maximum permissible limit of EU guideline. The microbiological analysis showed irrespective of RSV types, total aerobic bacteria was present in higher number (4.0-7.0 log CFU/g), whereas 3.36-5.57 log CFU/g coliform count was recorded. In comparison with retail markets, lower level of total aerobic, and coliform bacterial presence was observed in the samples collected from sophisticated shops, but presence of E. coli and Salmonella spp. were evident in more than 60% samples in these shops. However, 50% and 33% samples from wholesale and retail markets respectively were noticed to be contaminated with Staphylococcus spp. Irrespective of RSV types, isolated E. coli were found resistance to 2-5 different antibiotics, where Salmonella spp. isolated from cucumber and coriander leaves showed resistance against 4-8 different antimicrobials. Therefore, the study results demonstrated that, the presence of residual pesticides, multidrug resistant E. coli and Salmonella spp. in the RSV samples posing concern when consumed raw. The regulatory bodies are expected to monitor and ensure the overall quality standards are in place and practiced by food producers and marketers responsible for handling and distribution of RSVs.
