Population Structure and Genetic Diversity of the Pepper Weevil (Coleoptera: Curculionidae) Using the COI Barcoding Region.

The pepper weevil Anthonomus eugenii Cano (Coleoptera: Curculionidae) is a pest of economic importance for Capsicum species pepper in North America that attacks the reproductive structures of the plant. The insect is distributed across Mexico, the United States, and the Caribbean, and is occasionally found during the pepper growing season in southern Ontario, Canada. Continuous spread of the insect to new areas is partially the result of global pepper trade. Here, we describe the genetic diversity of the pepper weevil using the mitochondrial COI barcoding region across most of its geographic range. In this study, 44 (H1-H44) highly similar haplotypes were identified, the greatest number of haplotypes and haplotype diversity were observed among specimens from its native Mexico, followed by specimens from the United States. Unlike Mexico, a low haplotype diversity was found among specimens from Canada, the Dominican Republic, Italy, and the Netherlands. Out of these 44 haplotypes, 29 are reported for the first time. Haplotype diversity in the Canadian population suggests either multiple and continuous introductions of the pepper weevil into this area or a single introduction of genetically diverse individuals. We discuss the importance of such population genetic data in tailoring pepper weevil management programs, using Canada as an example.

Host utilization by the pepper weevil (Anthonomus eugenii): suitability, preference and offspring performance.

BACKGROUND: Host plant selection is a key factor affecting the survival, population establishment, and spread of herbivorous insect pests. The pepper weevil is one of the most important pests of cultivated pepper in North America with a capacity to rapidly expand its geographic range, in part through its ability to switch between cultivated and wild Solanaceous host plants. Towards a better management of this pest, this study examined metrics of pepper weevil host use including oviposition preference, development time, and successful offspring emergence on wild Solanaceous species and cultivated pepper cultivars. RESULTS: Pepper weevil successfully developed within fruit of several Solanaceous species including eastern black (Solanum ptycanthum) and climbing nightshade (S. dulcamara), in which development time was on average 4 days faster relative to Capsicum annuum cv. jalapeno peppers. Oviposition events occurred in all fruit types assessed and no strong host preference was detected among these. However, the number of emerged offspring was significantly lower than the number of oviposition events in C. chinense cv. habanero pepper fruit. CONCLUSION: Although not all nightshade species are suitable hosts for pepper weevil development, those permissive to offspring production do allow for faster development than in C. annuum peppers. While host preference was not detected among fruit types tested, low offspring emergence from fruit with high capsaicin content suggests a reduced ability of pepper weevil to tolerate high concentrations of this metabolite. These findings help elucidate the factors influencing pepper weevil bionomics, and their implications on pepper weevil management are discussed. Published 2021. This article is a U.S. Government work and is in the public domain in the USA.

A highly resolved food web for insect seed predators in a species-rich tropical forest.

The top-down and indirect effects of insects on plant communities depend on patterns of host use, which are often poorly documented, particularly in species-rich tropical forests. At Barro Colorado Island, Panama, we compiled the first food web quantifying trophic interactions between the majority of co-occurring woody plant species and their internally feeding insect seed predators. Our study is based on more than 200 000 fruits representing 478 plant species, associated with 369 insect species. Insect host-specificity was remarkably high: only 20% of seed predator species were associated with more than one plant species, while each tree species experienced seed predation from a median of two insect species. Phylogeny, but not plant traits, explained patterns of seed predator attack. These data suggest that seed predators are unlikely to mediate indirect interactions such as apparent competition between plant species, but are consistent with their proposed contribution to maintaining plant diversity via the Janzen-Connell mechanism.

Habitat or temporal isolation: Unraveling herbivore-parasitoid speciation patterns using double digest RADseq.

Ecological speciation is often observed in phytophagous insects and their parasitoids due to divergent selection caused by host-associated or temporal differences. Most previous studies have utilized limited genetic markers or distantly related species to look for reproductive barriers of speciation. In our study, we focus on closely related species of Lygus bugs and two sister species of Peristenus parasitoid wasps. Using mitochondrial DNA COI and genomewide SNPs generated using ddRADseq, we tested for potential effects of host-associated differentiation (HAD) or temporal isolation in this system. While three species of Lygus are clearly delineated with both COI and SNPs, no evidence of HAD or temporal differentiation was detected. Two Peristenus sister species were supported by both sets of markers and separated temporally, with P. mellipes emerging early in June and attacking the first generation of Lygus, while P. howardi emerging later in August and attacking the second generation of their hosts. This is one of the few studies to examine closely related hosts and parasitoids to examine drivers of diversification. Given the results of this study, the Lygus-Peristenus system demonstrates temporal isolation as a potential barrier to reproductive isolation for parasitoids, which could indicate higher parasitoid diversity in regions of multivoltine hosts. This study also demonstrates that incorporating systematics improves studies of parasitoid speciation, particularly by obtaining accurate host records through rearing, carefully delimiting cryptic species and examining population-level differences with genomic-scale data among closely related taxa.

Insect herbivory on seedlings of rainforest trees: Effects of density and distance of conspecific and heterospecific neighbors.

Natural enemies of plants such as insect herbivores can contribute to structuring and maintaining plant diversity in tropical forests. Most research in this area has focused on the role of specialized enemies and the extent to which herbivory on individual plant species is density-dependent. Relatively few insect herbivores specialize on a single host plant species. Insect herbivores that feed on more than one plant species may link the regeneration dynamics of their host species through "apparent competition" or "apparent mutualism." We investigated herbivory and survival of seedlings of two tropical tree species (Cordia alliodora and Cordia bicolor) in the forests of Barro Colorado Island (Panama). We used experiments and observations to assess seedling fate in relation to the presence of conspecifics and heterospecifics across a range of spatial scales. Herbivory significantly increased seedling mortality and was highest at high local densities of C. alliodora seedlings. There was also evidence that high local densities of C. alliodora increased herbivory on co-occurring C. bicolor seedlings. Synthesis. The elevated rates of seedling herbivory at high densities of conspecifics documented in our study are consistent with the predictions of the Janzen-Connell hypothesis, which explains how so many plant species can coexist in tropical forests. Our data also highlight the possibility that herbivore-mediated density-dependence, facilitated by herbivores that feed on multiple plant species, can also occur across plant species. Enemy-mediated indirect effects of this sort have the potential to structure plant communities.

Los enemigos naturales de plantas, tales como insectos herbívoros pueden contribuir a estructurar y mantener la diversidad arbórea en los bosques tropicales. La mayoría de investigaciones en ésta área se han centrado en el papel de enemigos especializados y en la medida en la que la herbivoría en plantas hospederas individuales depende de la densidad.Relativamente pocos insectos herbívoros se especializan en una única especie de planta hospedera. Los insectos herbívoros que se alimentan en más de una especie de planta pueden vincular la dinámica de regeneración de sus hospederas a través de "competencia aparente" o "mutualismo aparente".Nosotros investigamos la herbivoría y supervivencia de las plántulas de dos especies de árboles tropicales (Cordia alliodora y Cordia bicolor) en los bosques de la Isla de Barro Colorado (Panamá). Usamos experimentos y observaciones para evaluar el destino de las plántulas con relación a la densidad de conespecíficos y heteroespecíficos a través de un rango de escalas espaciales.La herbivoría aumentó significativamente la mortalidad de las plántulas, y fue significativamente más alta en densidades locales altas de C. alliodora. Hubo también evidencia que densidades locales altas de C. alliodora aumentaron la herbivoría en plántulas adyacentes de C. bicolor.Síntesis. Las elevadas tasas de herbivoría en plántulas a densidades altas de conespecíficos documentadas en nuestro estudio son consistentes con las predicciones de la hipótesis de Janzen­Connell, la cual provee una explicación a la alta diversidad de especies vegetales en los bosques tropicales. Nuestros datos también resaltan la posibilidad que efectos similares mediados por herbivoría puedan ocurrir a través de especies y sean facilitados por herbívoros especializados al nivel de género en lugar de especies. Efectos indirectos de ésta clase mediados por enemigos tienen el potencial de estructurar comunidades vegetales.