RESUMO
BACKGROUND: Regular cognitive training can boost or maintain cognitive and brain functions known to decline with age. Most studies administered such cognitive training on a computer and in a lab setting. However, everyday life activities, like musical practice or physical exercise that are complex and variable, might be more successful at inducing transfer effects to different cognitive domains and maintaining motivation. "Body-mind exercises", like Tai Chi or psychomotor exercise, may also positively affect cognitive functioning in the elderly. We will compare the influence of active music practice and psychomotor training over 6 months in Mild Cognitive Impairment patients from university hospital memory clinics on cognitive and sensorimotor performance and brain plasticity. The acronym of the study is COPE (Countervail cOgnitive imPairmEnt), illustrating the aim of the study: learning to better "cope" with cognitive decline. METHODS: We aim to conduct a randomized controlled multicenter intervention study on 32 Mild Cognitive Impairment (MCI) patients (60-80 years), divided over 2 experimental groups: 1) Music practice; 2) Psychomotor treatment. Controls will consist of a passive test-retest group of 16 age, gender and education level matched healthy volunteers. The training regimens take place twice a week for 45 min over 6 months in small groups, provided by professionals, and patients should exercise daily at home. Data collection takes place at baseline (before the interventions), 3, and 6 months after training onset, on cognitive and sensorimotor capacities, subjective well-being, daily living activities, and via functional and structural neuroimaging. Considering the current constraints of the COVID-19 pandemic, recruitment and data collection takes place in 3 waves. DISCUSSION: We will investigate whether musical practice contrasted to psychomotor exercise in small groups can improve cognitive, sensorimotor and brain functioning in MCI patients, and therefore provoke specific benefits for their daily life functioning and well-being. TRIAL REGISTRATION: The full protocol was approved by the Commission cantonale d'éthique de la recherche sur l'être humain de Genève (CCER, no. 2020-00510) on 04.05.2020, and an amendment by the CCER and the Commission cantonale d'éthique de la recherche sur l'être humain de Vaud (CER-VD) on 03.08.2021. The protocol was registered at clinicaltrials.gov (20.09.2020, no. NCT04546451).
Assuntos
COVID-19 , Disfunção Cognitiva , Música , Humanos , Idoso , Pandemias , Disfunção Cognitiva/psicologia , Cognição , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudos Multicêntricos como AssuntoRESUMO
The constant intensification of aquaculture has considerable increased the stress levels of farmed fish and, consequently, the number and intensity of diseases outbreaks. Thus, studies on fish immune response, especially regarding the interaction of fish leukocytes with potential pathogens and xenobiotics are of great importance in order to develop new prophylactic and curative strategies. We isolated leukocytes from the head kidney of Astyanax lacustris-an important Neotropical fish species for aquaculture and a potential model for Neotropical aquaculture research-using a Percoll centrifugation protocol. The isolated leukocytes were incubated with lipopolysaccharide (LPS), and the expression of genes IL-1ß, IL-8, LysC, and LysG were measured. We assessed the phagocytotic activity of leukocytes using Congo red-dyed yeast, a novel and cost-effective protocol that has been developed in this study. The isolated leukocytes responded to LPS induction, exhibiting strong IL-1ß and IL-8 upregulation, two of the most important pro-inflammatory interleukins for vertebrates immune reponse. The optimal concentration of yeast for the phagocytic assay was 106 cells mL-1, resulting in acceptable phagocytic capacity (PC) but without excess of yeasts during the counting process, ensuring a high precision and accuracy of the method. To the best of our knowledge, the present study is the first to investigate the in vitro gene expression and phagocytic activity of leukocytes isolated from A. lacustris. Our findings will serve as a reference for future studies on the immunology and toxicology of Neotropical fish.
Assuntos
Characidae , Animais , Characidae/genética , Expressão Gênica , Interleucina-8/metabolismo , Leucócitos/metabolismo , Lipopolissacarídeos/farmacologia , Lipopolissacarídeos/metabolismoRESUMO
1. An experiment was carried out to validate techniques as predictive diagnostic tools for breast myopathies and to study the allometric growth of distinct parts of the body and meat quality of broilers.2. Infrared thermography was performed at 35 d of age. The surface temperatures of breasts of 300 birds were recorded, followed by ultrasound imaging.3. The birds were slaughtered and the cuts were made to weigh the body parts. Then, the breasts were evaluated as for the presence and severity of myopathies, from which nine treatments were established represented by the associated degrees of the myopathies white striping and wooden breast and breasts classified as normal.4. There was no difference in surface temperatures and echogenicity values between normal breasts and breasts affected by myopathies. At 35 d of age few fillets classified as normal were found.5. The breast showed late growth in relation to the body, regardless of characteristic lesions of myopathies. The most severe score of wooden breast affected meat quality variables.
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Doenças Mamárias , Técnicas e Procedimentos Diagnósticos , Doenças Musculares , Doenças das Aves Domésticas , Animais , Galinhas , Carne/análise , Doenças Musculares/diagnóstico , Doenças Musculares/veterinária , Doenças das Aves Domésticas/diagnóstico , Doenças Mamárias/diagnóstico , Doenças Mamárias/veterinária , Qualidade dos Alimentos , Masculino , Feminino , Valor Preditivo dos TestesRESUMO
Dairy goats may rely heavily on body fat and protein reserves in early lactation. Therefore, we aimed to determine the energy requirement and estimate the efficiency of utilization the nutrients of tissues mobilized in the first 8â¯weeks of lactation for milk production using the comparative slaughter technique. The average initial body mass of 51 multiparous goats was 57.19⯱â¯8.38â¯kg and a body condition score of 3.0⯱â¯0.5. Three goats were slaughtered at the beginning of the experiment to serve as baseline animals to estimate initial empty BW and initial body composition. We used a complete randomized design in which the factor was the day of lactation for slaughtering (the 7th, 14th, 21st, 28th, 35th, 42nd, 49th and 56th day), with six repeats, totalling 48 goats. No fasting before slaughtering. All animals received a single experimental diet. The efficiency of transferring energy from body reserves to milk was estimated using a multiple linear regression equation yielding a value of 0.76. The total energy stored in the empty body decreased over the eight lactation weeks, from 726.47⯱â¯26.19 to 316.18⯱â¯49.21â¯MJ, a 56.47% reduction, mainly because of a reduction in the energy from internal fat of 3.96⯱â¯1.98â¯MJ/day. In conclusion, the net energy required for maintenance is 60⯱â¯30â¯kJ/BW0.75 per day, and the net energy required for lactation decreases 70⯱â¯30â¯kJ/day during the first eight lactation weeks.
Assuntos
Ração Animal , Metabolismo Energético , Ração Animal/análise , Animais , Peso Corporal , Dieta , Feminino , Cabras , Lactação , Leite , Necessidades NutricionaisRESUMO
The state of Bahia ranks fourth in the national rank for citrus production, and the region of Chapada Diamantina is emerging an important producer of orange for fresh fruit market. Huanglongbing (HLB) is the major phytosanitary threat to Bahia citriculture. In Brazil, the disease was first reported in 2004 in São Paulo state. The bacterium Candidatus Liberibacter asiaticus (CLas) is one of the causal agents of HLB, which is transmitted by the insect vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). Bahia is a HLB-free area; therefore, it is essential to monitor its citrus-producing areas to early detect any possible introduction of the CLas. This study aimed to monitor the presence of the bacteria in the insect vector. Diaphorina citri samples were collected from 2011 to 2014 in different cities located at Chapada Diamantina region and tested by qPCR for the presence of CLas. Three samples were considered positive to bacterium, and all from psyllids collected on Murraya paniculata in the city of Seabra.
Assuntos
Hemípteros/microbiologia , Insetos Vetores/microbiologia , Rhizobiaceae/isolamento & purificação , Animais , Brasil , Citrus/microbiologia , Doenças das Plantas/microbiologia , Rhizobiaceae/patogenicidadeRESUMO
Objetivou-se avaliar o poder preditivo do modelo do National Research Council (NRC) para gado leiteiro em estimar o consumo de matéria seca (CMS) por vacas mestiças, em pastagens tropicais. Foi efetuada uma análise conjunta de cinco estudos, contemplando três forrageiras. Foram avaliadas 132 estimativas individuais do CMS observado (CMSObs), obtidas por meio do indicador externo Cr2O3. O CMS também foi predito por meio do software do NRC (CMSPred), que, por sua vez, foi abastecido com inputs referentes aos animais e ao ambiente de criação. Os valores de CMSPred (12,7±1,6kg/d) foram semelhantes aos de CMSObs (12,3±3,3kg/d). Foram obtidas as seguintes estimativas da avaliação do poder preditivo do modelo: viés médio (-0,419kg/d), coeficiente de determinação (0,029), coeficiente de correlação (0,17; P=,051), quadrado médio do erro de predição (11,844±20,034), fator de eficiência do modelo (-0,081), coeficiente de determinação do modelo (4,1032) e fator de correção do viés (0,767). A comparação entre CMSObs e CMSPred permitiu identificar a tendência de superestimação das predições se considerado o ajuste por meio de regressão robusta para o modelo linear simples sem intercepto. Nas condições avaliadas, o modelo produz predições de CMS com satisfatória exatidão, porém com baixa precisão.(AU)
The aim of the present study was to evaluate the predictive power of estimating the dry matter intake (DMI) of crossbred cows on tropical pastures by the National Research Council (NRC) equation for dairy cattle. A joint analysis of five studies covering three forages was performed in which 132 individual estimates of observed DMI obtained through Cr2O3 as a marker. DMI was also predicted from the NRC (DMIPred) software with inputs concerning animals and breeding environment of the studies used. Predicted DMIPred average values (12.7±1.6kg/d) were similar to the observed DMIObs ones (12.3±3.3kg/d). We obtained the following estimates of the evaluation of the predictive power of the model: average bias (- 0.419kg/d), coefficient of determination (0.029), Person's correlation coefficient (0.17, P= 0.051), mean square error of prediction (11,844±20,034), model efficiency factor (- 0.081), coefficient of determination (4.1032), and bias correction factor (0.767). The comparison between DMIObs and DMIPred values allowed the identification of the overestimating tendency of the predictions demonstrated by the robust regression fit of the simple linear no intercept model. Nevertheless, the model yields predictions with satisfactory accuracy, but with low precision.(AU)
Assuntos
Animais , Feminino , Bovinos , Pastagens , Previsões/métodos , Ingestão de Alimentos , National Academy of Sciences, U.S.RESUMO
We have sought the molecular diagnosis of OI in 38 Brazilian cases through targeted sequencing of 15 candidate genes. While 71% had type 1 collagen-related OI, defects in FKBP10, PLOD2 and SERPINF1, and a potential digenic P3H1/WNT1 interaction were prominent causes of OI in this underrepresented population. INTRODUCTION: Defects in type 1 collagen reportedly account for 85-90% of osteogenesis imperfecta (OI) cases, but most available molecular data has derived from Sanger sequencing-based approaches in developed countries. Massively parallel sequencing (MPS) allows for systematic and comprehensive analysis of OI genes simultaneously. Our objective was to obtain the molecular diagnosis of OI in a single Brazilian tertiary center cohort. METHODS: Forty-nine individuals (84% adults) with a clinical diagnosis of OI, corresponding to 30 sporadic and 8 familial cases, were studied. Sixty-three percent had moderate to severe OI, and consanguinity was common (26%). Coding regions and 25-bp boundaries of 15 OI genes (COL1A1, COL1A2, IFITM5 [plus 5'UTR], SERPINF1, CRTAP, P3H1, PPIB, SERPINH1, FKBP10, PLOD2, BMP1, SP7, TMEM38B, WNT1, CREB3L1) were analyzed by targeted MPS and variants of interest were confirmed by Sanger sequencing or SNP array. RESULTS: A molecular diagnosis was obtained in 97% of cases. COL1A1/COL1A2 variants were identified in 71%, whereas 26% had variants in other genes, predominantly FKBP10, PLOD2, and SERPINF1. A potential digenic interaction involving P3H1 and WNT1 was identified in one case. Phenotypic variability with collagen defects could not be explained by evident modifying variants. Four consanguineous cases were associated to heterozygous COL1A1/COL1A2 variants, and two nonconsanguineous cases had compound PLOD2 heterozygosity. CONCLUSIONS: Novel disease-causing variants were identified in 29%, and a higher proportion of non-collagen defects was seen. Obtaining a precise diagnosis of OI in underrepresented populations allows expanding our understanding of its molecular landscape, potentially leading to improved personalized care in the future.
Assuntos
Osteogênese Imperfeita , Adulto , Brasil , Colágeno Tipo I/genética , Heterozigoto , Humanos , Mutação , Osteogênese Imperfeita/genética , Proteínas de Ligação a Tacrolimo/genéticaRESUMO
Brazil is home to a wide variety of flora, including several lesser known species, such as araçá that were processed in this study using two green technologies consecutively: supercritical fluid extraction (SFE) for nonpolar fraction and pressurized liquid extraction. For polar fraction, the experiments followed a central composite design involving ethanol as solvent, with temperature and static time in each bath as independent variables. Both extracts were analyzed for antioxidant and antimicrobial activities. Total phenolics content (TPC) was determined for all ethanol extracts. In the araçá essential oil (AEO) obtained by SFE, the extraction yield was 2.33%. The three major compounds of AEO were α-pinene (20.75%), p-cymene (20.50%), and o-cymene (20.05%). In ethanol extracts, the high yield (14.49%) was obtained at 74 °C/6 min and the major TPC (136.95 mg GAE/100 g) at 60 °C/9 min. Ethanol extracts presented good antioxidant activity (EC50 = 6.37 mg/mL) at 74 °C/6 min. AEO was unable to reduce DPPH⢠concentration by 50%. Both extract types presented an inhibitory effect against Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus cereus, while only the ethanol extracts presented effect against Listeria monocytogenes. This work had the aim to present the innovation of the use of a whole typical Brazilian fruit that gives rise to extracts with excellent properties for employment in both the pharmaceutical and food industries.
Assuntos
Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Cromatografia com Fluido Supercrítico/métodos , Química Verde/métodos , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Psidium/química , Antibacterianos/química , Antioxidantes/análise , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Bacillus cereus/efeitos dos fármacos , Brasil , Dióxido de Carbono/química , Cromatografia com Fluido Supercrítico/instrumentação , Etanol/química , Frutas/química , Química Verde/instrumentação , Óleos Voláteis/análise , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia , Fenóis/análise , Fenóis/isolamento & purificação , Fenóis/farmacologia , Extratos Vegetais/análise , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
It is suggested that bovine enteroviruses (BEV) are involved in the aetiology of enteric infections, respiratory disease, reproductive disorders and infertility. In this study, bovine faecal samples collected in different Brazilian states were subjected to RNA extraction, reverse transcription-polymerase chain reaction analysis and partial sequencing of the 5'-terminal portion of BEV. One hundred and three samples were tested with an overall positivity of 14.5%. Phylogenetic analysis clustered these BEV Brazilian samples into the Enterovirus F clade. Our results bring an important update of the virus presence in Brazil and contribute to a better understanding of the distribution and characterisation of BEV in cattle.
Assuntos
Doenças dos Bovinos/virologia , Infecções por Enterovirus/veterinária , Enterovirus Bovino/isolamento & purificação , Animais , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Infecções por Enterovirus/epidemiologia , Infecções por Enterovirus/virologia , Enterovirus Bovino/genética , FilogeniaRESUMO
AIM: To establish an SV40 T-Ag-transfected cell line of human pulp-derived cells in order to compare the cytotoxicity, genotoxicity and to investigate the activities of immunological biomarkers of several endodontic sealers. METHODOLOGY: Primary human pulp cells and transfected cells were cultured. Cell morphology and proliferation were analysed, and the expression of cell-specific gene transcripts and proteins was detected by RT-PCR and immunohistochemistry. Transfection of human pulp-derived cells resulted in an immortalized cell line retaining phenotypic characteristics from the primarily cells tested. The SV40 T-Ag-transfected cells were cultured and stimulated by sealers (Apexit Plus, Real Seal, AH Plus, and EndoREZ) to evaluate the cytotoxicity and genotoxicity by MTT and MTN assays, respectively. Immunological inflammatory biomarkers (IL6, IL8 and TNF-α) were determined by ELISA assay. The differences between median values were statistically analysed using Kruskal-Wallis and Dunn's tests at 5% significance level. RESULTS: The cytotoxicity assay revealed that multimethacrylate (Real Seal) was the most cytotoxic sealer (P < 0.05) and exhibited the highest inflammatory potential against the SV40 T-Ag-transfected cells (P < 0.05). All root canal sealers tested were able to stimulate the immortalized pulp cells to produce IL-6, IL-8 and TNF-α, with differences in relation to the control group (P < 0.05). Higher levels of IL-6, IL-8 and TNF-α were found in cell supernatant after stimulation with multimethacrylate (Real Seal) compared to all other sealers tested (P < 0.05). No differences were found comparing epoxy resin-based sealer (AHPlus), single-methacrylate sealer (EndoREZ) and calcium hydroxide-based sealer (Apexit Plus), regardless of the cytokine investigated (all P > 0.05). CONCLUSIONS: A SV40 T-Ag-transfected cell line of human pulp-derived cells was established. The methacrylate resin-based sealer (Real Seal) exhibited the greatest cytoxicity and inflammatory potential against immortalized pulp cells compared to an epoxy resin-based sealer (AH Plus), a methacrylate-based sealer (EndoRez) and a calcium hydroxide-based sealer (Apexit).
Assuntos
Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/toxicidade , Biomarcadores/análise , Células Cultivadas , Citocinas/análise , Humanos , Teste de Materiais , TransfecçãoRESUMO
The aim of this study was to evaluate the hepatoprotective effect of silymarin in diets contaminated or not with aflatoxin B1 (AFB1) on the productive performance and serum biochemical profile of Japanese quail (Coturnix coturnix japonica) in the laying phase. A total of 240 12-week-old Japanese quail was used in a completely randomized design in a 3 × 2 factorial scheme (additives x contaminated or not with AFB1 - 1,500 µg/kg), totaling 6 treatments and 5 replicates of 8 birds each. The additives used were silymarin (500 g/ton), adsorbent (1 kg/ton), and a control diet (without additive). Of the total aflatoxin content, 84.64% was AFB1; 4.28% was AFB2; 11.07% was AFG1; and AFG2 was not detected. The data were submitted to ANOVA, and means were compared by Tukey's test. There was no interaction (P > 0.05) between the additive and AFB1 on performance parameters. However, the inclusion of AF in diets reduced (P < 0.05) egg weight and feed intake, impairing feed conversion compared to the unchallenged groups. There was an increase (P < 0.05) in blood concentrations of aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), and creatine kinase (CK) in birds challenged with AFB1, regardless of the additive used, characterizing a possible alteration in hepatic metabolism. Serum total protein and globulin levels were reduced (P < 0.05) in birds challenged with toxins. The consumption of diets contaminated with 1,500 µg AFB1/kg altered hepatic function in quail, impairing productive performance and egg weight. The concentrations of silymarin and adsorbent evaluated in this study were not able to mitigate the negative effect of toxins on the metabolism and performance of laying quail.
Assuntos
Aflatoxina B1/efeitos adversos , Coturnix/fisiologia , Substâncias Protetoras/farmacologia , Silimarina/farmacologia , Aflatoxina B1/administração & dosagem , Ração Animal/análise , Animais , Proteínas Aviárias/sangue , Dieta/veterinária , Suplementos Nutricionais/análise , Substâncias Protetoras/administração & dosagem , Silimarina/administração & dosagemRESUMO
Mass spectrometry imaging (MSI) of animal tissues has become an important tool for in situ molecular analyses and biomarker studies in several clinical areas, but there are few applications in parasitological studies. Leishmaniasis is a neglected tropical disease, and experimental mouse models have been essential to evaluate pathological and immunological processes and to develop diagnostic methods. Herein we have employed MALDI MSI to examine peptides and low molecular weight proteins (2 to 20 kDa) differentially expressed in the liver during visceral leishmaniasis in mice models. We analyzed liver sections of Balb/c mice infected with Leishmania infantum using the SCiLS Lab software for statistical analysis, which facilitated data interpretation and thus highlighted several key proteins and/or peptides. We proposed a decision tree classification for visceral leishmaniasis with distinct phases of the disease, which are named here as healthy, acute infection and chronic infection. Among others, the ion of m/z 4963 was the most important to identify acute infection and was tentatively identified as Thymosin ß4. This peptide was previously established as a recovery factor in the human liver and might participate in the response of mice to Leishmania infection. This preliminary investigation shows the potential of MALDI MSI to complement classical compound selective imaging techniques and to explore new features not yet recognized by these approaches.
Assuntos
Leishmania infantum/patogenicidade , Leishmaniose Visceral/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Animais , Doenças do Cão/imunologia , Doenças do Cão/metabolismo , Cães , Leishmania infantum/imunologia , Leishmaniose/imunologia , Leishmaniose/metabolismo , Leishmaniose Visceral/imunologia , Camundongos , Camundongos Endogâmicos BALB C , SoftwareAssuntos
Doenças Transmissíveis Emergentes , Doenças da Laringe/diagnóstico , Doenças da Laringe/terapia , Leishmania braziliensis/isolamento & purificação , Leishmaniose/diagnóstico , Leishmaniose/terapia , Traqueostomia , Animais , Antiprotozoários/uso terapêutico , Emergências , Rouquidão/parasitologia , Humanos , Doenças da Laringe/parasitologia , Leishmaniose/complicações , Masculino , Meglumina/uso terapêutico , Antimoniato de Meglumina , Pessoa de Meia-Idade , Obstrução Nasal/parasitologia , Compostos Organometálicos/uso terapêutico , Psychodidae , Traqueostomia/métodos , Resultado do TratamentoRESUMO
The aim of this study was to verify the carryover of aflatoxin B1 from feed to lambari fish. Aflatoxins (AF) were incorporated into feed, checking the levels by HPLC. Treatments were: Control, feed without toxin; A, feed + 10 µg AFB1 kg-1; B, feed + 20 µg AFB1 kg-1; and C, feed + 50 µg AFB1 kg-1. Juveniles of lambari fish were placed in 12 aquariums at a density of 50 fish/m2. Fish were fed twice a day with extruded feed, at 5% of animal biomass. The unit sample was constituted by a pool of 10 fish. AFs B1, B2, G1, G2 and M1 were quantified by HPLC in fish muscle and liver after 30, 60, 90 and 120 days of experiment. There was accumulation of AFs is fish liver and muscle, mainly after 90 days. Fish from treatment C had higher levels of AFB1 in muscle when compared with the others, and AFB1 in muscle at 120 days was similar to the levels in feed. Therefore, when lambari fish is exposed on a daily and long-term basis to AFs in feed, the regulation limits for AFs in animal feed do not guarantee safety for consumers.
Assuntos
Aflatoxinas/análise , Ração Animal/análise , Contaminação de Alimentos/análise , Fígado/química , Músculos/química , Aflatoxinas/farmacocinética , Animais , Characidae , Cromatografia Líquida de Alta Pressão , Distribuição TecidualRESUMO
Nebovirus is a new genus of viruses belonging to the Caliciviridae family recently characterized in cattle, and is associated with gastrointestinal disorders, such as diarrhoea, anorexia and intestinal lesions particularly in calves. The aim of this study was to investigate the prevalence of neboviruses in Brazilian cattle and analyse phylogenetically the virus strains detected. A prevalence of 4·8% of neboviruses in faecal samples from 62 head of cattle from different Brazilian states was detected. All positive animals were aged 96·0% nt (100% aa) sequence identity between the virus sequences in this study and >88·8% nt (>94·4% aa) identity with Newbury1/UK. Our results indicate, for the first time, the occurrence of neboviruses in Brazil as well as in South America, and the first Newbury1-like nebovirus found outside the UK.
Assuntos
Infecções por Caliciviridae/veterinária , Caliciviridae/classificação , Caliciviridae/isolamento & purificação , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , Genótipo , Animais , Brasil/epidemiologia , Caliciviridae/genética , Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Bovinos , Análise por Conglomerados , Diarreia/epidemiologia , Diarreia/veterinária , Diarreia/virologia , Fezes/virologia , Feminino , Gastroenterite/epidemiologia , Gastroenterite/veterinária , Gastroenterite/virologia , Humanos , Masculino , Filogenia , Prevalência , Homologia de Sequência do Ácido NucleicoRESUMO
BACKGROUND AND OBJECTIVE: Procedural pain in neonates has been a concern in the last two decades. The purpose of this review was to provide a critical appraisal and a synthesis of the published epidemiological studies about procedural pain in neonates admitted to intensive care units. The aims were to determine the frequency of painful procedures and pain management interventions as well as to identify their predictors. DATABASES AND DATA TREATMENT: Academic Search, CINAHL, LILACS, Medic Latina, MEDLINE and SciELO databases were searched for observational studies on procedural pain in neonates admitted to intensive care units. Studies in which neonatal data could not be extracted from the paediatric population were excluded. RESULTS: Eighteen studies were included in the review. Six studies with the same study duration, the first 14 days of the neonate life or admission in the unit of care, identified 6832 to 42,413 invasive procedures, with an average of 7.5-17.3 per neonate per day. The most frequent procedures were heel lance, suctioning, venepuncture and insertion of peripheral venous catheter. Pharmacological and nonpharmacological approaches were inconsistently applied. Predictors of the frequency of procedures and analgesic use included the neonate's clinical condition, day of unit stay, type of procedure, parental presence and pain assessment. The existence of pain protocols was not a predictor of analgesia. CONCLUSIONS: Painful procedures were performed frequently and often with inadequate pain management. Unlike neonate clinical factors, organizational factors may be modified to promote a context of care more favourable to pain management.
Assuntos
Manejo da Dor , Dor/epidemiologia , Punções/efeitos adversos , Analgésicos/uso terapêutico , Cuidados Críticos , Humanos , Recém-Nascido , Estudos Observacionais como Assunto , Dor/diagnóstico , Dor/tratamento farmacológico , Medição da DorRESUMO
To assess the impact of the delay in recognition of the initial symptoms of laryngeal papillomatosis in children over the evolution of the disease. Retrospective study of patients with respiratory papillomatosis referred from general pediatric practices to a tertiary hospital with pediatric laryngology specialization. Gender, age at time of diagnosis, symptom duration, sites affected at the time of diagnosis, treatment, and evolution of the disease over time were evaluated. From January 2003 to December 2013, 21 patients (15 females and 6 males) were identified and followed for at least 3 years. The average age at which symptoms first appeared was 40.2 months, and the average age at the time of initial treatment was 76 months. The most frequent clinical manifestation was hoarseness. The most common site of involvement was the glottis followed by the supraglottis and subglottis, respectively. Three of the 21 patients required tracheostomy. The average time from symptom onset to diagnosis was 52.3 months. There were no fatalities. On average 3.7 procedures were performed per patient. Patients presenting more than 1 year from the symptom onset to the time of first treatment required a greater number of procedures to control disease. Delay in diagnosis can have negative clinical consequences due to disease progression. The later the diagnosis the more surgeries are needed to control the disease. Involvement of the subglottic larynx is a risk factor for emergent tracheostomy.
Assuntos
Diagnóstico Tardio , Neoplasias Laríngeas/diagnóstico , Neoplasias Laríngeas/patologia , Papiloma/diagnóstico , Papiloma/patologia , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Neoplasias Laríngeas/cirurgia , Estudos Longitudinais , Masculino , Papiloma/cirurgia , Estudos Retrospectivos , TraqueostomiaRESUMO
The aim of this study was to detect and quantify fumonisin B1 (FB1) in cereal mixtures marketed in Brazil. Fifteen samples from different lots were acquired each month by internet from supermarkets during seven months, adding up to 105 analysed samples. The unit sample constituted of an original package with a minimum of 250 g. Extraction and clean-up of samples for FB1 determination were carried out using immunoaffinity columns. Identification and quantification of FB1 were performed by high performance liquid chromatography. Eighty-eight (83.8%) samples were contaminated with FB1 and four (3.8%) presented levels above 500 µg kg(-1) (634, 703, 1269 and 1876 µg kg(-1)). Maximum FB1 + FB2 levels allowed by Brazilian regulations will reach 1500 µg kg(-1) for corn flour in 2016 and 1000 µg kg(-1) for others corn products. This study showed that even at levels below the legislative limits, human exposure to this toxin can occur constantly.
Assuntos
Grão Comestível/química , Fumonisinas/análise , Brasil , Cromatografia de Afinidade , Cromatografia Líquida de Alta Pressão , Limite de Detecção , Reprodutibilidade dos TestesRESUMO
Reducing spoilage and indicator bacteria is important for microbiological stability in meat and meat products. The objective was to evaluate the effect of different doses of gamma radiation on the shelf-life of lamb meat, vacuum-packed and stored under refrigeration, by assessing the microbiological safety, physicochemical stability and sensory quality. Lamb loin cuts (Longissimus dorsi) were irradiated with 1.5kGy and 3.0kGy. The samples, including control, were stored at 1±1°C during 56days. Samples were analyzed on zero, 14, 28, 42 and 56days by their microbiological and physicochemical characteristics. Sensory quality was carried out on day zero. The results showed a reduction (p<0.05) in the microbial load of the irradiated samples. The acceptance of lamb loins was not affected (p>0.05) by the radiation doses. Thus gamma irradiation at 3.0kGy was effective in reducing the content of microorganisms, without harming the physicochemical characteristics evaluated.
Assuntos
Bactérias/efeitos da radiação , Microbiologia de Alimentos , Embalagem de Alimentos , Conservação de Alimentos , Raios gama , Carne/análise , Refrigeração , Animais , Contagem de Colônia Microbiana , Armazenamento de Alimentos , Humanos , Carne/microbiologia , Carne/normas , Músculo Esquelético , Odorantes , Carneiro Doméstico , Paladar , VácuoRESUMO
Introduction: Currently, two types of atlas provide anatomical studies: those with two-dimensional images, and those compiled by interactive pictures; mostly drawn virtually. In recent decades, the development of virtual anatomical atlases has been encouraged and prioritized by both the technological advances and the need to facilitate and complement medical education. The aim of this study was to develop an interactive virtual atlas of the upper limbs. Material and Methods: A systematic dissection of three upper limbs (shoulder, arm, forearm and hand) of legally preserved cadavers in Anatomy Laboratory of São José do Rio Preto Medical School, SP (FAMERP) has been carried out. Results: A website has been developed to facilitate the learning of superficial and deep structures of the limbs comprising ten pictures showing the steps and the various structures and display devices (naming demarcated structures and text with relevant information about these). In addition, the website includes a photo gallery with subtitled images of the dissected upper limbs. Conclusion: In conclusion, virtual study of Anatomy by means of interactive-image display of dissected parts can facilitate and enhance the learning of the upper limbs.(AU)