Chemical Composition and Evaluation of Antibacterial, Antibiofilm, and Mutagenic Potentials of a Propolis Sample from the Atlantic Forest of Midwest Brazil.

Sixteen triterpenoids with various skeletal types, five phenylpropanoid derivatives, and two flavonoids were isolated from a propolis sample produced by Apis mellifera collected in the Atlantic Forest of Midwest Brazil. Among these compounds, six triterpenes, namely 3ß,20R-dihydroxylanost-24-en-3-yl-palmitate, (23E)-25-methoxycycloartan-23-en-3-one, 24-methylenecycloartenone, epi-lupeol, epi-α-amyrin, and epi-ß-amyrin are being reported for the first time in propolis, while cycloartenone, (E)-cinnamyl benzoate, and (E)-cinnamyl cinnamate are new findings in Brazilian propolis. The presence of cycloartane- and lanostane-type triterpenoids, the latter being a class of compounds of restricted distribution in propolis worldwide, has not been reported in propolis from Midwest Brazil until now. The ethyl acetate phase obtained from the ethanol extract was effective in preventing biofilm formation by Staphylococcus aureus, with an inhibition rate of about 96 % at 0.5 mg.mL-1 , and with quercetin isolated as one of its active constituents. In contrast, the hexane phase exhibited notable antibacterial activity against Pseudomonas aeruginosa, inhibiting bacterial growth by 92 % at 0.5 mg.mL-1 ; however, none of the triterpenoids isolated from this phase proved active against this pathogen. The ethanol extract was neither toxic nor mutagenic at the concentrations tested, as determined by the in vivo SMART assay on Drosophila melanogaster, even under conditions of high metabolic activation.

Prevention measures for vertical HIV transmission: monitoring infected pregnant women and exposed children / Medidas de prevenção para transmissão vertical do HIV: acompanhamento de gestantes infectadas e crianças expostas

Abstract Objectives: to present an analysis of the follow-up of exposed children and pregnant women living with HIV who are assisted in a reference public healthcare service of a Brazilian capital. Methods: this is a cross-sectional study with sociodemographic and clinical data obtainedfrom physical and electronic medical records of women living with HIV and exposed children, treated between 2000 and 2018, in a secondary healthcare service. For associations, the chi-square test at 5% significance was used. Results: the data about 183 pregnancies and 214 children from the capital or other municipalities in the state were analyzed. The women have low schooling, are brown or black, single, multiparous, diagnosed with HIV during prenatal care, with the start of follow-up from the third trimester of pregnancy and the use of prophylaxis during childbirth. As for children, the associations were significant for notification on SINAN, admission to the health service, use of prophylaxis indicating better monitoring for those who live in the state capital. Conclusions: the prevention measures recommended by the Ministry of Health, in general, were followed, but children and pregnant women who live in other cities were worse off than those ones in the capital. Unnotified cases were identified on SINAN. These issues may contribute to new cases of vertical transmission of HIV.

Resumo Objetivos: apresentar uma análise do acompanhamento de crianças expostas e gestantes vivendo com HIV atendidas em um serviço público de referência de uma capital brasileira. Métodos: trata-se de estudo transversal, com dados sociodemográficos e clínicos obtidos em prontuários físicos e eletrônicos mulheres vivendo com HIV e crianças expostas, atendidas entre 2000 a 2018 em um serviço de atenção secundária à saúde. Para as associações foi utilizado o teste qui-quadrado, com significância de 5%. Resultados: foram analisados dados de 183 gestações de 150 mulheres e 214 crianças expostas a infecção pelo HIV As mulheres possuíam baixa escolaridade, eram pardas ou pretas, solteiras, multíparas, com diagnóstico de HIV durante o pré-natal, com início do acompanhamento a partir do terceiro trimestre de gestação e uso de profilaxia durante o parto. Quanto às crianças, as associações foram significativas para notificação no SINAN, admissão no serviço de saúde, uso de profilaxia indicando melhor acompanhamento para aquelas que moravam na capital do estado. Conclusões: as medidas de prevenção recomendadas pelo Ministério da Saúde, em geral, foram seguidas, porém apresentaram pior cenário as crianças e gestantes que residiam em outros munícipios quando comparadas as da capital. Foram identificados casos não notificados no SINAN. Essas questões podem contribuir para novos casos de transmissão vertical do HIV.

Osteopoiquilose: comparação entre a radiografia e a ressonância magnética / Osteopoiquilosis: comparison between radiography and magnetic resonance

A osteopoiquilose é uma displasia osteosclerótica rara que mostra uma transmissão autossômica dominante e tem características radiológicas específicas que não apresenta clínica específica; o diagnóstico é feito por achados radiológicos incidentais típicos em pacientes que procuram tratamento para outros problemas médicos. A osteopoiquilose pode ser confundida com metástases osteoblásticas, esclerose tuberosa e mastocitose óssea. Relatamos uma mulher de 37 anos queixando-se de dor e edema no punho há 20 dias. A paciente foi submetida a avaliação com ressonância magnética, apresentando ossos do carpo e regiões epifisárias do rádio e ulna, além de metacarpos e algumas falanges, com diversos focos escleróticos dispersos. Em seguida, para nova avaliação, a paciente realizou radiografias de joelhos, ombros e punhos, confirmando os focos escleróticos encontrados na ressonância magnética, confirmando o diagnóstico de osteopoiquilose

Osteopoikilosis is a rare osteosclerotic dysplasia that shows an autosomal dominant transmission and has characteristic radiological features, there are no specific clinical features; diagnosis is made by incidental typical radiological findings in patients seeking treatment for other medical problems. Osteopoikilosis can be confused with osteoblastic metastases, tuberous sclerosis, and bone mastocytosis. We report a 37-years-old woman, complaining of pain and swelling in the wrist 20 days ago. The patient submitted to MRI for evaluation, showing carpal bones and epiphyseal regions of radius and ulna, as well as metacarpal and some phalanges, presenting several scattered sclerotic foci. After that for further evaluation, the patient was referred for radiographs of the knees, shoulders, and wrists, confirming the sclerotic foci found in MRI, confirming the diagnosis of osteopoikilosis

Lesão renal no trauma abdominal fechado ­ diagnóstico pelos exames de imagem / Renal injury in closed abdominal trauma ­ diagnosis by imaging tests

A lesão do trato geniturinário ocorre em aproximadamente 10% dos casos de trauma renal, sendo que a lesão renal ocorre em aproximadamente 1% a 5% de todos os casos, apresentando predominância masculina ­ 3:1. As lesões renais podem ser classificadas em abertas ou fechadas ­ 80-95% dos casos são fechadas em países desenvolvidos; em países subdesenvolvidos 59% das lesões são abertas. A tomografia computadorizada (TC) é o padrão-ouro. No entanto, devido a crescente preocupação com a exposição à radiação, principalmente em crianças, a ultrassonografia é uma excelente alternativa para a avaliação de lesões do trato urinário. O objetivo deste estudo é relatar dois casos de trauma renal, um em um paciente adulto e outro em um paciente pediátrico, destacando a importância da avaliação pela ultrassonografia e pela TC

Injury of the genitourinary tract occurs in approximately 10% of cases of renal trauma, with renal injury occurring in approximately 1% to 5% of all cases, with a male predominance - 3:1. Kidney injuries can be classified as blunt or penetrating - 80-95% of cases are blunt in developed countries; in underdeveloped countries, 59% of injuries are penetrating. Computed tomography (CT) scan is the gold standard. However, due to the growing concern with radiation exposure, especially in children, ultrasound is an excellent alternative for the evaluation of urinary tract injuries. This study aims to report two cases of renal trauma, one in an adult patient and the other in a pediatric patient, highlighting the importance of evaluation by ultrasound and CT scan

Síndrome do osso navicular acessório / Accessory navicular bone syndrome

O osso navicular acessório, do qual foram descritas três variantes, é frequentemente considerado uma variação anatômica normal.É uma anomalia congênita em que a tuberosidade do navicular se desenvolve a partir de um centro de ossificação secundário e tem uma incidência estimada entre 2% e 14% com maior incidência em mulheres sendo bilateral em 50-90% dos casos. Nosso objetivo foi relatar um caso e revisar a literatura acerca desta lesão incomum, utilizando-se da revisão do prontuário, registro fotográfico do método diagnóstico e revisão da literatura. Mulher de 59 anos com dor no lado medial do pé esquerdo há dois anos, com piora ao andar. Exame físico com testes específicos do tornozelo negativos, sem hematoma ou edema. Nega torção, trauma e cirurgias. A ressonância magnética demonstra osso navicular acessório tipo II e osso navicular, ambos com edema. Estes achados com quadro clínico são compatíveis com síndrome do osso navicular acessório. Este relato demonstra a dificuldade de diagnosticar essa lesão, pois seu o exame físico é inespecífico, podendo simular patologias mais complexas, necessitando de exames complementares para seu correto diagnóstico (AU)

The accessory navicular bone, of which three variants have been described, is often considered as a normal anatomic and roentgenographic variant. Is a congenital anomaly in which the tuberosity of the navicular bone develops from a secondary ossification center and it has an incidence estimated between 2% and 14% with higher incidence in women being bilateral in 50-90% of the cases. Our aim was to report a case and review the literature about this uncommon lesion. We carried out a review of medical records, photographic record of diagnostic method, and review from the literature. A 59 years old woman with pain in the medial side of the left foot for two years. Refers worsening with walking. Physical examination with specific ankle negative tests, without bruise or edema. Denies torsion, trauma and surgeries. Magnetic resonance imaging demonstrates type II accessory navicular bone with edema and navicular bone edema. These findings with clinical complaint are compatible with accessory navicular bone syndrome. This report demonstrates the difficulty of diagnosing this lesion, since its physical examination is non-specific, and it can simulate more complex pathologies, requiring complementary tests for its correct diagnosis (AU)

Copper exposure and seawater acidification interaction: Antagonistic effects on biomarkers in the zooxanthellate scleractinian coral Mussismilia harttii.

Coral reefs are threatened by global and local impacts, such as ocean acidification (OA) and metal contamination. Toxicity of metals, such as copper (Cu), is expected to be enhanced with OA. However, the interaction between these environmental stressors is still poorly evaluated. In the present study, the interactive effects of seawater acidification and increasing Cu concentrations were evaluated in a zooxanthellate scleractinian coral (Mussismilia harttii), using biochemical biomarkers involved in the coral calcification process and the photosynthetic metabolism of endosymbionts. Corals were kept under control conditions (no seawater acidification and no Cu addition in seawater) or exposed to combined treatments of reduced seawater pH (8.1, 7.8, 7.5 and 7.2) and environmentally relevant concentrations of dissolved Cu (measured: 1.0, 1.6, 2.3 and 3.2 µg/L) in a mesocosm system. After 15- and 35-days exposure, corals were analyzed for photochemical efficiency (Fv/Fm), chlorophyll a content, Ca-ATPase and carbonic anhydrase (CA) activity. Results showed that 76% of the interactions between reduced seawater pH and increasing Cu concentrations were antagonistic. Only 24% of these interactions were additive or synergistic. In general, the combination of stressors had no significant deleterious effects in the photosynthetic metabolism of endosymbionts or Ca-ATPase activity. In fact, the lowest dissolved Cu concentration tested had a consistent positive effect on Ca-ATPase activity in corals facing any of the reduced seawater pH conditions tested. In turn, potentially deleterious effects on acid-base balance in M. harttii, associated with changes in CA activity, were intensified by the combination of stressors. Findings reported here indicate that Cu toxicity in future OA scenarios can be less severe than previously suggested in this coral holobiont.

Dyke-Davidoff-Masson syndrome and chiari type ii malformation / Síndrome de Dyke-Davidoff-Masson e malformação de chiari tipo II

Dyke-Davidoff-Masson Syndrome is a syndrome associated with refractory epilepsy. The Chiari II malformation is a complex congenital malformation of the brain. The authors report a case of a 15 years-old adolescent presenting Dyke-Davidoff-Masson syndrome and Chiari type II malformation association. This case demonstrates an unusual association in neuroimaging tests that indicates the need to evaluate associated diseases, such as myelomeningocele, corpus callosum dysgenesis and syringohydromyelia.

A Síndrome de Dyke-Davidoff-Masson é uma síndrome associada à epilepsia refratária. A malformação de Chiari II é uma malformação congênita complexa do cérebro. Os autores relatam um caso de uma adolescente de 15 anos apresentando a síndrome de Dyke-Davidoff-Masson associada à malformação de Chiari tipo II. Este caso demonstra uma associação incomum nos exames de neuroimagem que indica a necessidade de avaliar doenças associadas, como mielomeningocele, disgenesia do corpo caloso e a siringohidromielia.

A rare case of gastric mucormycosis in an immunocompetent patient.

We report the case of a 23-year-old immunocompetent patient who presented at the emergency department of a Brazilian hospital with epigastric pain and fever. After an investigation that included a computed tomography scan and upper gastrointestinal endoscopy with biopsy, a diagnosis of mucormycosis was established. The patient exhibited favorable progress after surgery and antifungal therapy. Mucormycosis is a rare condition that usually affects immunocompromised patients, with a high mortality rate of up to 85%. Correct diagnosis and fast initiation of therapy are required to ensure improved patient prognosis.

Cisto no ligamento cruzado anterior: relato de caso de um achado raro / Anterior cruciate ligament cyst ­ A rare finding

Cistos no ligamento cruzado anterior são difíceis de diagnosticar devido à falta de sintomas e sinais específicos. A incidência deste transtorno é mais alta entre 20 e 40 anos de idade, ocorrendo predominantemente em homens. Nosso objetivo foi relatar um caso e revisar a literatura acerca desta lesão incomum, através de revisão do prontuário, registro fotográfico do método diagnóstico e revisão da literatura. Homem de 18 anos com dor no joelho direito há 04 meses. Refere jogar duas horas de futebol todos os dias por alguns anos. Exame físico com testes específicos do joelho negativos, sem hematoma ou edema. Nega torção, trauma e cirurgias. A ressonância magnética demonstra cisto lobulado intrasubstancial no ligamento cruzado anterior. Este relato demonstra a dificuldade de diagnosticar essa lesão, pois o exame físico é inespecífico, podendo simular patologias mais graves, necessitando de exames complementares para seu correto diagnóstico.

Cysts in the anterior cruciate ligament is difficult to diagnose due to the lack of specific symptoms and signs. Incidence of this disorder was the highest for 20­40 years of age group. Occurs predominantly in males. Our aim was to report a case and review the literature about this uncommon lesion. We carried out a review of medical records, photographic record of diagnostic method, and review from the literature. 18 years-old man with pain in the right knee for 04 months. He plays two hours of soccer everyday for a few years. Physical examination with specific knee negative tests, without bruise or edema. Denies torsion, trauma and surgeries. Magnetic resonance imaging demonstrates intrasubstantial lobulated cyst in the anterior cruciate ligament. This report demonstrates the difficulty of diagnosing this lesion, since its physical examination is non-specific, and it can simulate more serious pathologies, requiring complementary tests for its correct diagnosis.

A rare case of gastric mucormycosis in an immunocompetent patient

Abstract We report the case of a 23-year-old immunocompetent patient who presented at the emergency department of a Brazilian hospital with epigastric pain and fever. After an investigation that included a computed tomography scan and upper gastrointestinal endoscopy with biopsy, a diagnosis of mucormycosis was established. The patient exhibited favorable progress after surgery and antifungal therapy. Mucormycosis is a rare condition that usually affects immunocompromised patients, with a high mortality rate of up to 85%. Correct diagnosis and fast initiation of therapy are required to ensure improved patient prognosis.

Rim em Panqueca ­ Um Achado Incomum / Pancake Kidney ­ An Unusual Finding

Introdução: o rim em panqueca foi descrito pela primeira vez por Looney e Dodd (1926), mas apresenta outras nomenclaturas como "bolo", "disco", "rosca" e "rim em escudo", porque produz uma massa em forma de anel ou em forma de rosca. É uma ectopia renal congênita fundida muito rara. Objetivo: relatar um caso raro de rim em panqueca associado a apendicite. Materiais e Métodos: revisão do prontuário, registro fotográfico dos métodos diagnósticos e revisão da literatura. Resultados: jovem de 15 anos de idade, do sexo masculino, com dor em cólica no hipogástrio há dois dias com vômitos há um dia. A tomografia computadorizada demonstrou apendicite e uma massa lobulada fundida situada na cavidade pélvica que se estende de L2 a L4 com ureteres inserindo-se separadamente na bexiga urinária, caracterizando rim em panqueca. Conclusão: relatamos um caso de rim em panqueca, entidade muito rara e pouco relatada na literatura médica, muitas vezes insuspeitada na avaliação clínica inicial, porém que pode causar complicações, tais como a litíase renal e a hipertensão arterial.

Introduction: pancake kidney was first described by Looney and Dodd, but presents other nomenclatures such as "cake", "disk", "doughnut" and "shield kidney" because it produces a ring-shaped or doughnut-shaped mass. It is a very rare fused congenital renal ectopia. Objective: to report a rare case of pancake kidney associated with appendicitis. Materials and Methods: We carried out a review of medical records, photographic record of diagnostic methods and review from the literature. Results: 15 year old male patient with colic pain in the hypogastrium two days ago with vomiting a day ago. Computed tomography scan showed appendicitis and a fused lobulated mass located in the pelvic cavity extending from L2 to L4 with ureters inserting separately into the urinary bladder, characterizing pancake kidney. Conclusion: pancake kidney is an entity very rare and little reported in the medical literature, often unsuspected in the initial clinical evaluation, but that can lead to complications such as renal lithiasis and arterial hypertension.

A comparison of the prevalence of fluorosis in 8-year-old children from seven European study sites using a standardized methodology.

OBJECTIVES: The objectives of this study were to report on the prevalence of enamel opacities from seven European study sites using a standardized photographic method, and to investigate the importance of variables responsible for enamel fluorosis. METHODS: The sample comprised a randomly selected group of 300 8-year-old children in each of the study areas. One examiner from each area was trained and calibrated in the use of a standardized photographic technique. Two transparencies were taken of each child's permanent maxillary central incisor teeth; one to represent the teeth 'wet' and one when the teeth had been allowed to dry out naturally for 105 s. The transparencies were viewed 'blind' by the author (JAC) and scored using the DDE and TF indices. Data relating to variables considered to be associated with enamel fluorosis were also collected. RESULTS: The prevalence of diffuse opacities ranged from 61% in fluoridated Cork (Ireland) to 28% in Athens (Greece). The percentage of subjects with a TF score of three or more ranged from 4% in Cork and nonfluoridated Haarlem (the Netherlands) to zero in Oulu (Finland) and Athens. Fluoridated water and the prolonged use of fluoride tablets were found to be significant contributory factors to fluorosis. CONCLUSIONS: The prevalence of fluorosis was found to be highest in fluoridated Cork. The prolonged use of fluoride supplements was also found to be a significant risk indicator associated with fluorosis.

Fluoride ingestion from toothpaste: conclusions of European Union-funded multicentre project.

An important challenge encountered in this multicentred project was the need to take account of the different cultural and legal differences between the seven sites when agreeing the protocol. Examples such as access to registers of births and subject consent dictated that there were some differences in the methods used in the different sites. The data presented showed that it was possible to train and calibrate a number of examiners in a standardized photographic method for recording enamel fluorosis. This method has a number of important advantages for the objective monitoring of enamel fluorosis over time. There were considerable differences between the seven sites in the formulations of the toothpaste used and in the pattern of their use. The results indicate that it is possible to agree and adopt a standardized method for measuring fluoride ingestion from toothpaste. The aesthetic impact of enamel fluorosis seemed low in the populations included in this project, but further work is required on this issue.