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1.
PLoS One ; 15(11): e0241546, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33151992

RESUMO

Here we present and analyze the complete genome of Alcaligenes faecalis strain Mc250 (Mc250), a bacterium isolated from the roots of Mimosa calodendron, an endemic plant growing in ferruginous rupestrian grasslands in Minas Gerais State, Brazil. The genome has 4,159,911 bp and 3,719 predicted protein-coding genes, in a single chromosome. Comparison of the Mc250 genome with 36 other Alcaligenes faecalis genomes revealed that there is considerable gene content variation among these strains, with the core genome representing only 39% of the protein-coding gene repertoire of Mc250. Mc250 encodes a complete denitrification pathway, a network of pathways associated with phenolic compounds degradation, and genes associated with HCN and siderophores synthesis; we also found a repertoire of genes associated with metal internalization and metabolism, sulfate/sulfonate and cysteine metabolism, oxidative stress and DNA repair. These findings reveal the genomic basis for the adaptation of this bacterium to the harsh environmental conditions from where it was isolated. Gene clusters associated with ectoine, terpene, resorcinol, and emulsan biosynthesis that can confer some competitive advantage were also found. Experimental results showed that Mc250 was able to reduce (~60%) the virulence phenotype of the plant pathogen Xanthomonas citri subsp. citri when co-inoculated in Citrus sinensis, and was able to eradicate 98% of juveniles and stabilize the hatching rate of eggs to 4% in two species of agricultural nematodes. These results reveal biotechnological potential for the Mc250 strain and warrant its further investigation as a biocontrol and plant growth-promoting bacterium.


Assuntos
Alcaligenes faecalis/genética , Citrus/microbiologia , Genoma Bacteriano , Sequenciamento Completo do Genoma , Alcaligenes faecalis/efeitos dos fármacos , Animais , Antibacterianos/farmacologia , Sequência de Bases , Citrus/parasitologia , DNA Circular/genética , Resistência Microbiana a Medicamentos/efeitos dos fármacos , Resistência Microbiana a Medicamentos/genética , Ilhas Genômicas/genética , Ferro/metabolismo , Metais Pesados/toxicidade , Mimosa/microbiologia , Nematoides/fisiologia , Fenóis/metabolismo , Filogenia
2.
Radiol. bras ; Radiol. bras;44(2): 104-108, mar.-abr. 2011. ilus, tab
Artigo em Português | LILACS | ID: lil-588196

RESUMO

OBJETIVO: O objetivo deste trabalho foi verificar a aceitabilidade para a implantação no Brasil de um banco de objetos simuladores nacional, ou bancos regionais, para uso compartilhado desses objetos em atividades de controle de qualidade nos serviços de medicina nuclear. MATERIAIS E MÉTODOS: Foram analisadas as respostas dadas em um questionário que foi enviado a supervisores de radioproteção e físicos médicos de serviços de medicina nuclear do Brasil. Inicialmente, o questionário foi validado por profissionais da cidade de Aracaju, SE, que está localizada no Nordeste. De acordo com as regiões geográficas brasileiras, fizeram parte da amostra investigada: o Nordeste, com respostas de profissionais de 13 serviços de medicina nuclear; o Norte, com 2 profissionais; o Sul, com 7 profissionais; o Sudeste, com 43 profissionais; e o Centro-Oeste, com 2 profissionais. RESULTADOS: Segundo os dados analisados, 82 por cento dos entrevistados consideram que a implantação de um banco de simuladores seria uma alternativa apropriada para o aprimoramento do controle de qualidade em medicina nuclear. O interesse em compartilhar com o banco foi de 87 por cento. CONCLUSÃO: Os resultados mostraram que há motivação para o compartilhamento de objetos simuladores, ou seja, para o uso desses objetos de forma socializada.


OBJECTIVE: The present study was aimed at determining the acceptability of a national or regional phantoms bank to be deployed in Brazil for shared use in quality control activities by nuclear medicine centers. MATERIALS AND METHODS: The authors analyzed the answers to a questionnaire applied to medical physicists and radioprotection supervisors in Brazilian nuclear medicine centers. Initially, the questionnaire was validated by professionals in the city of Aracaju, SE, which is located in the Northeast region. The present study sample was geographically distributed as follows: the Northeast region, with answers from 13 professionals of nuclear medicine centers; the North region, with 2 professionals; the South region, with 7 professionals; the Southeast region, with 43 professionals; and the Midwest region, with 2 professionals. RESULTS: According to the data analyzed, 82 percent of the respondents consider that the implementation of a phantoms bank would be a suitable alternative for improving nuclear medicine quality control. The interest in sharing the bank was reported by 87 percent of the respondents. CONCLUSION: The present study demonstrated the motivation for a shared use of a future phantoms bank.

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