Synthesis of lignin-based carbon/graphene oxide foam and its application as sensors for ammonia gas detection.

The present study highlights the integration of lignin with graphene oxide (GO) and its reduced form (rGO) as a significant advancement within the bio-based products industry. Lignin-phenol-formaldehyde (LPF) resin is used as a carbon source in polyurethane foams, with the addition of 1 %, 2 %, and 4 % of GO and rGO to produce carbon structures thus producing carbon foams (CFs). Two conversion routes are assessed: (i) direct addition with rGO solution, and (ii) GO reduction by heat treatment. Carbon foams are characterized by thermal, structural, and morphological analysis, alongside an assessment of their electrochemical behavior. The thermal decomposition of samples with GO is like those having rGO, indicating the effective removal of oxygen groups in GO by carbonization. The addition of GO and rGO significantly improved the electrochemical properties of CF, with the GO2% sensors displaying 39 % and 62 % larger electroactive area than control and rGO2% sensors, respectively. Furthermore, there is a significant electron transfer improvement in GO sensors, demonstrating a promising potential for ammonia detection. Detailed structural and performance analysis highlights the significant enhancement in electrochemical properties, paving the way for the development of advanced sensors for gas detection, particularly ammonia, with the prospective market demands for durable, simple, cost-effective, and efficient devices.

Selected Kraft lignin fractions as precursor for carbon foam: Structure-performance correlation and electrochemical applications.

The rapid exhaustion of fossil fuels brings to the fore the need to search for energy efficient strategies. The conversion of lignin into advanced functional carbon-based materials is considered one of the most promising solutions for environmental protection and the use of renewable resources. This study analyzed the structure-performance correlation of carbon foams (CF) when lignin-phenol-formaldehyde (LPF) resins produced with different fractions of kraft lignin (KL) were employed as carbon source, and polyurethane foam (PU) as sacrificial mold. The lignin fractions employed were KL, fraction of KL insoluble in ethyl acetate (LFIns) and fraction of KL soluble in ethyl acetate (LFSol). The produced CFs were characterized by thermogravimetric analysis (TGA), X-ray diffractometry (XRD), Raman spectroscopy, 2D HSQC Nuclear magnetic resonance (NMR) analysis, scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and electrochemical measurements. The results showed that when LFSol was employed as a partial substitute for phenol in LPF resin synthesis, the final performance of the produced CF was infinitely higher. The improved solubility parameters of LFSol along with the higher S/G ratio and ß-O-4/α-OH content after fractionation were the key to produce CF with better carbon yields (54 %). The electrochemical measurements showed that LFSol presented the highest current density (2.11 × 10-4 mA.cm-2) and the lowest value of resistance to charge transfer (0.26 KΩ) in relation to the other samples, indicating that the process of electron transfer was faster in the sensor produced with LFSol. LFSol's potential for application as an electrochemical sensor was tested as a proof of concept and demonstrated excellent selectivity for the detection of hydroquinone in water.

Biodegradable and Flexible Thermoplastic Composite Graphite Electrodes: A Promising Platform for Inexpensive and Sensitive Electrochemical Detection of Creatine Kinase at the Point-of-Care.

Acute myocardial infarction (AMI) is the main cause of death worldwide, and the time of diagnosis is decisive for the effectiveness of the treatment of patients with AMI. Creatine kinase-myocardial band (CK-MB) has a predominance and high affinity with myocardial tissue, making it considered one of the main biomarkers for the diagnosis of AMI. In this work, we report a novel biodegradable composite material based on a polymer blend of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Poly(butylene adipate-co-terephthalate) (PHBV:Ecoflex) and graphite microparticles for sensitive and selective electrochemical detection of CK-MB. The morphological and physicochemical characterizations of the thermoplastic composite material revealed a homogeneous and synergistic distribution of the graphite microparticles through the blend structure, providing low defects and high electrical conductivity with high electron transfer kinetics (k0 = 3.54 × 10-3 cm s-1) features with adequate flexibility for point-of-care applications. The portable and disposable devices were applied to detect CK-MB using the electrochemical impedance spectroscopy (EIS) technique in a relevant clinical concentration ranging from 5.0 ng mL-1 to 100.0 ng mL-1 and presented a limit of detection of 0.26 ng mL-1 CK-MB. The selectivity of the sensor was confirmed by testing the potential interference of major biomolecules found in biofluids and other relevant macromolecules. The accuracy and robustness were assessed by addition and recovery protocol in urine and saliva samples without sample pretreatment and demonstrated the potential of our method for rapid and decentralized tests of AMI. In addition, the study of the thermal, biological, and photodegradation of the devices after being used was also carried out, aiming at the disposal of the material more sustainably.

Improvement of UV stability of thermoplastic starch matrix by addition of selected lignin fraction - Photooxidative degradation.

This paper examines the additivation of thermoplastic starch (TPS) matrix by selected fractions of kraft lignin (KL) and correlates its structure-performance when exposed to photooxidative degradation. KL from Eucalyptus urograndis wood was refined by a sequential fractionation process in ethyl acetate (EtOAc). Films were prepared by mixing lignin fractions as additive in TPS matrix by casting and pressing. The lignin employed were KL, fraction of KL insoluble in EtOAc (INS) and fraction of KL soluble in EtOAc (SOL). The samples were exposed to accelerated aging with Ultraviolet-C light (UV-C) for 432 h. Structural changes were measured by FTIR (Fourier-Transform Infrared) spectra. Thermal properties, such as melting enthalpy, glass transition temperature and thermal decomposition, were evaluated by DSC (Differential Scanning Calorimetry) and TG (Thermogravimetry). Morphology of the films was obtained by SEM (Scanning Electron Microscopy). Surface property of wettability was measured by contact angle. Mechanical properties were explored before and after exposure to UV-C light. It was observed that the least photodegraded films were those resulting from the addition of the lignin fraction with higher phenolic hydroxyl group content. According to structural and morphological observations, the soluble fraction (TSOL) presented the highest photoprotection and stabilizing effect as an UV-C light blocker additive on TPS matrix.

Improvements in thermal and mechanical properties of composites based on thermoplastic starch and Kraft Lignin.

Thermoplastic starch (TPS) is a widely studied biopolymer as an alternative to the use of conventional polymers. In this sense, the incorporation of fillers or reinforcements coming preferably from other substances of natural origin, can be an alternative to try to improve some mechanical and thermal properties of starch polymers. Thus, Kraft Lignin (KL), can be an excellent filler to be incorporated, since it presents mechanical and thermal properties and reduces the cost and weight of the final compounds. TPS films were prepared by casting using dimethyl sulfoxide (DMSO) as solvent and additives with 2, 4 and 8% KL. Characterization of TPS films and compositions with KL were carried out by Fourier-Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Dynamic Thermomechanical Analysis (DMTA), tensile testing and contact angle. Samples were also analyzed for biodegradation and for the ability to remove contaminants in water, Metil Orange (MO), by Ultraviolet-Visible Spectroscopy (UV-Vis). The FT-IR spectra of the films showed bands typical of functional groups derived from starch and lignin, with the intensity of these bands varying among the samples studied. Micrographs revealed slightly different morphologies among the films, but all showed irregular shapes with structures that appeared as plots. Increasing the percentage of KL led to an increase in contact angle values, showing a more hydrophobic behavior. In the TGA analysis, it was possible to observe a change in the main degradation event of the films for lower temperatures, especially of TPS - 4 and 8% KL compared to the TPS film. Films with KL had the peak of maximum degradation shifted to temperatures below the starch film, where the decrease in intensity of the main peak in the TPS - 4% KL and TPS - 8% KL samples demonstrates that there was less mass loss in the event. There was also in the percentage of residue as the addition of KL was increased The DMTA analyses allowed for the conclusion that presence of KL in TPS film allowed for an increase in its energy storage property, and that the loss modulus followed a decreasing order of storage modulus values to TPS - 8% KL from TPS. For the tensile strength property only TPS - 4% KL has significant improvement, and the elongation at break showed an increase for TPS - 4 and 8% KL compared to TPS. Samples showed a continuous and progressive biodegradation process, being completely biodegraded within 10 days. The monitoring of the ability to remove contaminants from water by UV-Vis, also showed promising results of compounds for this application. The best results were obtained, in most tests, for the TPS- 4% KL films.

Disposable and low-cost electrochemical sensor based on the colorless nail polish and graphite composite material for tartrazine detection.

A new method to manufacture electrochemical devices based on the graphite and colorless nail polish (N-grap) film was developed for tartrazine (Tz) detection. Scanning Electron Microscopy (SEM) demonstrates that the composite material presents a high porous carbon structure. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were employed to electrochemically characterize the electrode material, which corroborates the porous structure of the N-graph due to the enhanced electroactive area (5.4-fold increase) and presented a heterogeneous electron transfer rate constant (k0) of 5.82 × 10-3 cm s-1 for potassium ferricyanide. The electrochemical determination of the Tz was carried out using square-wave voltammetry (SWV), under the optimized experimental conditions, which showed high sensitivity (0.793 A L mol-1) and a lower limit of detection (LOD) of 2.10 × 10-8 mol L-1 with a linear concentration ranging from 2.0 to 50.0 µmol L-1. The developed sensor was applied for the analysis of Tz in sports drink samples and the result obtained by N-grap device was statistically compared with a spectrophotometric method demonstrating good accordance and the accuracy of the proposed method. Based on these results, we believe that this new fabrication method to produce disposable and low-cost electrochemical devices can be an alternative method for in-field analysis of dye in commercial sport drink samples and other relevant applications.

Interfacial behavior of Lactate Oxidase at Air-Subphase interface.

This article investigates the main aspects of the surface chemistry properties of the lactate oxidase (LacOx) enzyme monolayer at the air-subphase interface. Surface chemistry study determined the important properties like the surface packing and stability of the formed layer, whereas the spectroscopic experiments provided information regarding its secondary structure conformation of the enzyme. We have demonstrated that the LacOx in the monolayer form remained active for extended time period. In accordance to the data obtained from the isotherm it was also found that LacOx forms a stable monolayer that does not aggregate at the air-subphase interface. The stability of the monolayer at the air-subphase interface was studied by using compression-decompression cycles which revealed the stability with no significant evidence of aggregates or irreversible domains. This was further confirmed by UV-vis absorption and fluorescence measurements. Spectra from circular dichroism (CD) showed that the LB film retains the characteristic of an α-helix conformation.

A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders.

A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP461-70, AQP4131-140, AQP4141-150, and AQP4201-210) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP461-70-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP461-70 sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP461-70-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.

Improving direct immunoassay response by layer-by-layer films of gold nanoparticles - Antibody conjugate towards label-free detection.

The conjugation of nanoparticles with antibodies has been successfully applied in sandwich immunoassays for detecting cancer biomarkers. However, two antibodies are necessary to perform such experiment, being one of them functionalized with a signal label for optical or electrochemical assay. This approach is time and cost consuming compared to direct label-free immunoassays. In this study, we propose the synthesis of gold nanoparticles conjugated to anti-PSA antibody to produce a label-free impedimetric immunosensors based on nanostructured Layer-by-Layer (LbL) films. Prostate-specific antigen (PSA) detection was performed by electrochemical impedance spectroscopy demonstrating a detection mechanism governed by Langmuir-Freundlich adsorption model. This strategy provided a significant sensitivity using 10-fold less antibody than conventional immunosensors, i.e. decreasing costs using a simple approach, with a limit of detection of 0.17 ng mL-1 and an analytical range of 0.1-20 ng mL-1 indicating that our sensor is potentially useful for clinical applications.

Layer-by-Layer Films of Gold Nanoparticles and Carbon Nanotubes for Improved Amperometric Detection of Cholesterol.

Distinct architectures of layer-by-layer (LbL) films made of carbon nanotubes and gold nanoparticles were investigated to serve as the matrix to immobilize cholesterol oxidase, with which cholesterol could be detected using amperometry. The gold nanoparticles were synthesized by metal reduction stabilized in poly(allylamine hydrochloride) (PAH) providing a stable AuNPs-PAH suspension, while multi-walled carbon nanotubes (CNTs) were functionalized with carboxylic groups to obtain an aqueous suspension. The LbL films were deposited on ITO, with a cushion film of PAH and poly(vinyl sulfonic acid) (PVS). Owing to the synergy between CNTs and AuNPs, the electrode ITO/(PAH/PVS)2 (AuNPs-PAH/CNTs)10 was selected for immobilization of cholesterol oxidase (ChOx). This sensor could detect cholesterol with a limit of detection of 14.8 µmol L-1 and sensitivity of 36.47 (µA cm-2)·(mmol L-1)-1. It was also able to determine cholesterol in egg yolk with a recovery of 97.7%.

Regioregularity and deposition effect on the physical/chemical properties of polythiophene derivatives films.

Polythiophene thin films are widely studied for applications in organic electronics. However, some comparisons are still missing, regarding distinct deposition techniques and regioregularity. Here regioregular and regiorandom alkyl-substituted polythiophene derivatives (P3ATs) were deposited on solid substrates using both Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) techniques. The main goal was to verify the possible influence of the regioregularity as well the deposition technique on their optical, electrical and electrochemical properties. LB and LS films of regioregular and regiorandom poly(3-butylthiophene) (P3BT) and poly(3-octylthiophene) (P3OT) were deposited onto glass/Indium-Tin-Oxide) substrates and characterized by UV-visible optical spectroscopy, atomic force microscopy, cyclic voltammetry, and conductivity measurements. The results demonstrated the influence of the deposition technique on the electrical outcome, moreover, the regioregularity affected all the performed characterizations. In addition, this paper may be useful to understand how the amphiphilic molecule addition affected the film properties of regioregular and regiorandom P3ATs, particularly the energy diagram provided by the electrochemical and absorption features.

Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes.

Antigen-antibody interaction is crucial in autoimmune disease pathogenesis, as multiple sclerosis and neuromyelitis optica. Given that, autoantibodies are essential biomolecules, of which the myelin oligodendrocyte glycoprotein (MOG) can figure as a target. Here we combined Molecular Dynamics (MD), Steered Molecular Dynamics (SMD), and Atomic Force Microscope (AFM) to detail MOG recognition by its specific antibody. The complex model consisted of the MOG external domain interacting with an experimental anti-MOG antibody from the Protein Data Bank (1PKQ). Computational data demonstrated thirteen MOG residues with a robust contribution to the antigen-antibody interaction. Comprising five of the thirteen anchor residues (ASP102, HIS103, SER104, TYR105, and GLN106), the well-known MOG92-106 peptide in complex with the anti-MOG was analysed by AFM and SMD. These analyses evidenced similar force values of 780 pN and 765 pN for computational and experimental MOG92-106 and anti-MOG detachment, respectively. MOG92-106 was responsible for 75% of the total force measured between MOG external domain and anti-MOG, holding the interaction with the antibody. The antigen-antibody binding was confirmed by Surface Plasmon Resonance (SPR) measurements. Combined approaches presented here can conveniently be adjusted to detail novel molecules in diseases research. This can optimize pre-clinical steps, guiding experiments, reducing costs, and animal model usage.

Label-free, spatially multiplexed SPR detection of immunoassays on a highly integrated centrifugal Lab-on-a-Disc platform.

As a direct, label-free method, Surface Plasmon Resonance (SPR) detection significantly reduces the needs for liquid handling and reagent storage compared to common enzyme-linked immunosorbent assays (ELISAs), thus enabling comprehensive multiplexing of bioassays on microfluidic sample-to-answer systems. This paper describes a highly integrated centrifugal Lab-on-a-Disc (LoaD) platform for automating the full process chain extending between plasma extraction and subsequent aliquoting to five parallelized reaction channels for quantitative SPR detection by an inexpensive smartphone camera. The entire, multi-step / multi-reagent operation completes within less than 1 h. While the emphasis of this work is on the fluidic automation and parallelization by previously introduced, very robust event-triggered valving and buoyancy-driven centripetal pumping schemes, we successfully implement an immunoglobulin G (IgG) assay; by specific functionalization of the detection surfaces, the same disc layout can readily be customised for immunoassays panels from whole blood.

Photoswitchable Layer-by-Layer Coatings Based on Photochromic Polynorbornenes Bearing Spiropyran Side Groups.

Herein, we present the synthesis of linear photochromic norbornene polymers bearing spiropyran side groups (poly(SP-R)) and their assembly into layer-by-layer (LbL) films on glass substrates when converted to poly(MC-R) under UV irradiation. The LbL films were composed of bilayers of poly(allylamine hydrochloride) (PAH) and poly(MC-R), forming (PAH/poly(MC-R)) n coatings. The merocyanine (MC) form presents a significant absorption band in the visible spectral region, which allowed tracking of the LbL deposition process by UV-vis spectroscopy, which showed a linear increase of the characteristic MC absorbance band with increasing number of bilayers. The thickness and morphology of the (PAH/poly(MC-R)) n films were characterized by ellipsometry and scanning electron microscopy, respectively, with a height of ∼27.5 nm for the first bilayer and an overall height of ∼165 nm for the (PAH/poly(MC-R))5 multilayer film. Prolonged white light irradiation (22 h) resulted in a gradual decrease of the MC band by 90.4 ± 2.9% relative to the baseline, indicating the potential application of these films as coatings for photocontrolled delivery systems.

Layer-by-layer films containing emodin or emodin encapsulated in liposomes for transdermal applications.

Dermal drug release systems are an important area of research because they can be applied to the skin in a non-invasive procedure using a lower concentration of drugs. In this study, we have developed two types of Layer-by-Layer (LbL) films for releasing emodin (EM). In one system, EM was intercalated with poly(ethylenimine) PEI and poly(vinyl sufonate) (PVS) polyelectrolytes, forming (PEI/PVS)2(PEI/EM)7; in another, EM was incorporated in liposomes obtained by mixing dipalmitoyl phosphatidyl glycerol (DPPG) and palmitoyl oleoyl phosphatidyl glycerol (POPG) lipids, forming (PEI/PVS)2(PEI/DPPG-POPG-EM)7. UV-vis and FTIR spectroscopies were used to characterize the LbL films. These showed that the depositions of material by LbL were efficient, with increases in the absorbance of each bilayer evidencing the presence of EM in the film. The (PEI/PVS)2(PEI/EM)7 and (PEI/PVS)2(PEI/DPPG-POPG-EM)7 films released EM in three and five days, respectively. The cyclic voltammetry (CV) assay of the (PEI/PVS)2(PEI/EM)7 results are in agreement with UV-vis measurements, which suggest that EM was protonated in acid environments, while the CV of (PEI/PVS)2(PEI/DPPG-POPG-EM)7 demonstrated distinct protonation behaviour for EM within the inner liposome structure, even in acid solutions. Therefore, this study presents two systems based on LbL films and provides additional details about the release of EM from these films to create a viable alternative for transdermal applications.

Polyethylene imine/graphene oxide layer-by-layer surface functionalization for significantly improved limit of detection and binding kinetics of immunoassays on acrylate surfaces.

Antibody immobilization on polymeric substrates is a key manufacturing step for microfluidic devices that implement sample-to-answer automation of immunoassays. In this work, a simple and versatile method to bio-functionalize poly(methylmethacrylate) (PMMA), a common material of such "Lab-on-a-Chip" systems, is proposed; using the Layer-by-Layer (LbL) technique, we assemble nanostructured thin films of poly(ethylene imine) (PEI) and graphene oxide (GO). The wettability of PMMA surfaces was significantly augmented by the surface treatment with (PEI/GO)5 film, with an 81% reduction of the contact angle, while the surface roughness increased by 600%, thus clearly enhancing wettability and antibody binding capacity. When applied to enzyme-linked immunosorbent assays (ELISAs), the limit of detection of PMMA surface was notably improved from 340pgmL-1 on commercial grade polystyrene (PS) and 230pgmL-1 on plain PMMA surfaces to 130pgmL-1 on (PEI/GO)5 treated PMMA. Furthermore, the accelerated antibody adsorption kinetics on the LbL films of GO allowed to substantially shorten incubation times, e.g. for anti-rat IgG adsorption from 2h down to 15min on conventional and treated surfaces, respectively.

Surface plasmon resonance biosensor for enzymatic detection of small analytes.

Surface plasmon resonance (SPR) biosensing is based on the detection of small changes in the refractive index on a gold surface modified with molecular recognition materials, thus being mostly limited to detecting large molecules. In this paper, we report on a SPR biosensing platform suitable to detect small molecules by making use of the mediator-type enzyme microperoxidase-11 (MP11) in layer-by-layer films. By depositing a top layer of glucose oxidase or uricase, we were able to detect glucose or uric acid with limits of detection of 3.4 and 0.27 µmol l-1, respectively. Measurable SPR signals could be achieved because of the changes in polarizability of MP11, as it is oxidized upon interaction with the analyte. Confirmation of this hypothesis was obtained with finite difference time domain simulations, which also allowed us to discard the possible effects from film roughness changes observed in atomic force microscopy images. The main advantage of this mediator-type enzyme approach is in the simplicity of the experimental method that does not require an external potential, unlike similar approaches for SPR biosensing of small molecules. The detection limits reported here were achieved without optimizing the film architecture, and therefore the performance can in principle be further enhanced, while the proposed SPR platform may be extended to any system where hydrogen peroxide is generated in enzymatic reactions.

Layer-by-layer assembly of functionalized reduced graphene oxide for direct electrochemistry and glucose detection.

We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS. Stable aqueous dispersions of GPDDA and GPSS are easily obtained, enabling the growth of LbL films on various solid supports. The use of graphene in the immobilization of GOx promoted Direct Electron Transfer, which was evaluated by Cyclic Voltammetry. Amperometric measurements indicated a detection limit of 13.4µmol·L(-1) and sensitivity of 2.47µA·cm(-2)·mmol(-1)·L for glucose with the (GPDDA/GPSS)1/(GPDDA/GOx)2 architecture, whose thickness was 19.80±0.28nm, as determined by Surface Plasmon Resonance (SPR). The sensor may be useful for clinical analysis since glucose could be detected even in the presence of typical interfering agents and in real samples of a lactose-free milk and an electrolyte solution to prevent dehydration.

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection.

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (µA cm(-2))/(mmol L(-1)) and a detection limit of 0.33 mmol L(-1).