Insights into the degradation capacities of Amycolatopsis tucumanensis DSM 45259 guided by microarray data.

The analysis of catabolic capacities of microorganisms is currently often achieved by cultivation approaches and by the analysis of genomic or metagenomic datasets. Recently, a microarray system designed from curated key aromatic catabolic gene families and key alkane degradation genes was designed. The collection of genes in the microarray can be exploited to indicate whether a given microbe or microbial community is likely to be functionally connected with certain degradative phenotypes, without previous knowledge of genome data. Herein, this microarray was applied to capture new insights into the catabolic capacities of copper-resistant actinomycete Amycolatopsis tucumanensis DSM 45259. The array data support the presumptive ability of the DSM 45259 strain to utilize single alkanes (n-decane and n-tetradecane) and aromatics such as benzoate, phthalate and phenol as sole carbon sources, which was experimentally validated by cultivation and mass spectrometry. Interestingly, while in strain DSM 45259 alkB gene encoding an alkane hydroxylase is most likely highly similar to that found in other actinomycetes, the genes encoding benzoate 1,2-dioxygenase, phthalate 4,5-dioxygenase and phenol hydroxylase were homologous to proteobacterial genes. This suggests that strain DSM 45259 contains catabolic genes distantly related to those found in other actinomycetes. Together, this study not only provided new insight into the catabolic abilities of strain DSM 45259, but also suggests that this strain contains genes uncommon within actinomycetes.

Un análisis documental de la investigación en dislexia en la edad adulta / A Documentary Analysis of Research in Dyslexia in Adulthood

La dislexia en la edad adulta afecta alrededor del 4% de la población. Por ello, el objetivo de este trabajo es analizar los aspectos metodológicos, así como los temas de investigación, acerca de la dislexia en población adulta. A partir de una búsqueda bibliográfica en la base de datos del Psychological Abstracts: PsycoINFO, se identifican y seleccionan los trabajos sobre los que se centra la revisión. Los resultados indican respecto a la metodología que la mayoría de estudios emplean métodos cuantitativos de investigación (59.4%), frente al 5.7% que emplea métodos cualitativos. También se emplean en el 10% de los trabajos diseños de N = 1, y, en menos del 10%, entrevistas, estudios longitudinales y técnicas neurobiológicas. En cuanto a los temas de investigación, el 40.6% de los trabajos se centran en analizar procesos cognitivos (conciencia fonológica, memoria, inteligencia, velocidad de procesamiento) y el rendimiento académico (lectura de palabras, lectura de pseudopalabras, ortografía y comprensión de textos), el 25.4% son estudios neurobiológicos (neurológicos y genéticos), el 12% se centran en la intervención, el 8% en identificación y el 5.4% analizan los problemas asociados a la dislexia en la edad adulta. Los objetivos prioritarios de la investigación parecen ser la caracterización de las manifestaciones de la dislexia en la edad adulta, seguidos de los estudios que se dirigen al esclarecimiento de las bases neurobiológicas. También se amplía la investigación hacia temas de orden práctico.

Dyslexia in adulthood affects around 4% of the population. Thus, this study offers an overview of the methodological aspects and the areas of research in dyslexia in adulthood. From a bibliographical search conducted in the Psychological Abstracts database: PsycINFO, we identified and selected the studies the review was to focus on. Results show in relation to methodological aspects, quantitative research methods are preferred in 59.4% of cases, whereas 5.7% opted for qualitative methods. Also, in 10% of the studies, used N=1 designs and in an under 10% studies used surveys, longitudinal/ follow-up studies or neurobiological procedures. In relation to the topics of research, 40.6% analyzed cognitive processes (phonemic awareness, memory, IQ, naming speed) and/or academic achievement (word reading, pseudoword reading, spelling, and reading comprehension), the 25.4% were neurobiological studies (neurological and genetics), the 12% were studies focusing on intervention, the 8% focused on identification/ diagnostic, and 5.4% analyzed associated problems to dyslexia in adulthood. The primary objectives of the research appear to be the characterisation of dyslexia and studies about neurobiological basis of dyslexia in adulthood. Research is also extended towards other, more practical matters.

Knowledge and beliefs about developmental dyslexia in pre-service and in-service Spanish-speaking teachers.

The present study investigated knowledge, misconceptions, and lack of information about dyslexia among pre-service (PST) and in-service (IST) Spanish-speaking teachers in Spain and Peru. Two hundred and forty-six pre-service teachers and 267 in-service teachers completed the Knowledge and Beliefs about Developmental Dyslexia Scale (KBDDS). In-service teachers scored significantly higher on the total scale, and on the symptoms/diagnosis and general information subscales, than pre-service teachers. The percentages for misconceptions and lack of information ("do not know responses") were higher for PSTs than for ISTs on the general information subscale, the symptoms/diagnosis subscale, and the treatment subscale. Analyses of individual items were conducted to differentiate concepts that teachers did not know from misconceptions. In-service teacher self-efficacy, years of teaching experience, post-graduate training in dyslexia, and prior exposure to a child with dyslexia were positively related to knowledge about dyslexia. Implications for pre-service teacher training and professional development are discussed.

Narraciones escritas en niños con Trastorno Específico del Lenguaje (TEL) / Written Narration in Specific Language Impairment (SLI)

El presente estudio examinó las narraciones escritas, así como la influencia de las variables lingüísticas sobre la competencia narrativa de los niños con Trastorno Específico del Lenguaje (TEL). Veinticinco niños con TEL y 25 niños con desarrollo normal del lenguaje, equilibrados en edad e inteligencia, realizaron una tarea de narración escrita. Los resultados indican que ambos grupos difieren en la producción de narraciones. Los niños con TEL no solo escribieron historias más cortas, sino también de menor organización y coherencia. Además cometieron significativamente más errores de ortografía natural y de sintaxis. Los análisis de regresión jerárquica mostraron que diferentes medidas lingüísticas -memoria secuencial auditiva, comprensión auditiva y expresión verbal- tienen un peso predictivo de la competencia narrativa.

The present study examined the written narrations and the influence of linguistic measures on narrative competence of children with specific language impairment (SLI). A narration task was used to assess narrative abilities in 25 children with SLI and 25 normally developing children, matched on age and IQ. Results indicated that the two groups did differ in the production of narratives. The children with SLI provided short narratives, poorly organized and less cohesive. Also, writings were characterized by more syntax errors and had high proportion of phonologically inaccurate spelling errors. Hierarchical regression analysis showed that different linguistic measures - auditory comprehension, verbal fluency and sequential auditory memory - played a predictive role in narrative competence.

Functional-based screening methods for lipases, esterases, and phospholipases in metagenomic libraries.

The use of metagenomic techniques for enzyme discovery constitutes a powerful approach. Functional screens, in contrast to sequence homology search, enable us to select enzymes based on their activity. It is noteworthy that they additionally guarantee the identification of genes coding for enzymes that exhibited no sequence similarity to known counterparts from public databases and that even do not match any putative catalytic residues, involved in the selected catalytic function. Therefore, this strategy not only provides new enzymes for new biotechnological applications, but also allows functional assignment of many proteins, found in abundance in the databases, currently designated as "hypothetical" or "conserved hypothetical" proteins. In the past decade, there has been an exponential increase in the design of functional screening programmes, the majority of them established for hydrolases and oxidoreductases. Here, functional screening methods that guarantee the greatest enzyme diversity, for mining esterases and lipases, are described.