Is There a Relationship Between Birth Quartile and Vertical Jump Performance Among Young Male Elite Volleyball Athletes?

Vertical jump is an important skill that influences volleyball performance. In this study, we analyzed the relationship between vertical jump performance and birth quartile of Brazilian male youth volleyball players. We calculated chi-square goodness-of-fit tests to compare the athletes' birthdate distributions in quarters of their birth years (Q1, Q2, Q3, and Q4) according to player age categories (U17, U18, U19, and U21). We calculated one-way ANOVAs to compare spike jump and block jump heights of players born in different quarters of the same year. Overall, we found a relative age effect (i.e., more players with birth dates early in the birth year) in U17 (p < .001), U18 (p < .001), U19 (p < .001), and U21 (p = .04). Regarding vertical jump performance, U18 athletes born in Q2 reached higher spike jump heights (p = .006) and block jump heights (p = .002) than athletes born in Q4, and U19 athletes born in Q1 reached higher block jump heights than athletes born in Q3 (p = .049). There were no significant differences in vertical jump performance across birth quartiles among U17 and U21 athletes. Thus, a relative age effect was present in all age categories but not always reflected in vertical jump performance. Volleyball coaches and policymakers are still advised to employ strategies to ensure fairer opportunities for players born later in the year of their eligibility dates, as we found RAE to be sometimes, but not always, related to higher spike or block jump heights even among these older adolescents and young adult athletes.

Antifungal edible coatings for fruits based on zein and chitosan nanowhiskers.

Fresh produce have a more limited shelf life than processed ones. Their sensory attributes such as appearance and surface texture are important features in consumer perception and liking. The decomposition of fresh produce, which is caused by enzymes, chemical reactions, and microbial infections, often caused by Colletotrichum species, is inevitable. However, it can be slowed down. Several materials have been developed for this purpose, with an emphasis on active coatings using nanomaterials. In this study, the protective effects of a zein coating containing chitosan nanowhiskers (CSW) for the maintenance of fruit quality were investigated using guava (Psidium guajava L.) as a model fruit. CSW were previously characterized, and their antifungal effects against distinct Colletotrichum species (Colletotrichum asianum, Colletotrichum tropicale, Colletotrichum gloeosporioides, and Colletotrichum brevisporum) were proven. Coatings were characterized by thermogravimetric analysis, optical profilometry, and mechanical properties. Total soluble solids, pH, mass loss, and visual inspection of uncoated and coated guava fruits were also verified during 9 days. Results show that CSW length and aspect ratio decreased for longer extraction times. A similar behavior was found for x-ray diffraction in which peak intensity decreases under the same conditions. CSW degradation (ca. 250-400°C) also depends on extraction time in which more crystalline whiskers are the most thermally stable ones. The addition of CSW did not significantly (p < 0.05) modify the homogeneity and continuity of coating but prevented microbial growth assuring fruit quality during storage. In summary, coatings protected guava fruits from post-harvest spoilage while preserving quality and extending shelf life. PRACTICAL APPLICATION: Fresh foods such as fruits and vegetables have a more limited shelf life than processed ones.

The Decision-Making of High-Level Volleyball Setters in the 2021-2022 Volleyball Men's Superliga: Does the Opponent Matter?

Our primary objective in this study was to investigate the offensive strategies employed in the attack phase of men's volleyball, specifically focusing on side-out as stratified by the type of confrontation that was determined by the opponent's team performance. We analyzed 5524 attacking actions during 22 games of the 12 teams that participated in the Volleyball Men's Superliga (season 2021-2022). Based on their final rankings in the championship, we classified these teams into three tiers: high-performance, intermediate-performance, and low-performance. Subsequently, we examined the dynamics of these matches using Social Network Analysis. We found that the opponent teams' performance levels did not influence the game dynamics. Notably, the eigenvector values were prominently higher for Attack Zones 2 and 4, wherein the middle-blocker jumped to attack close to the setter across all networks. Thus, setters opted for traditional and low-risk strategies to minimize errors, disregarding available information about the skill level of the opposing team, making their offensive tactics predictable.

Targeting ryanodine receptor type 2 to mitigate chemotherapy-induced neurocognitive impairments in mice.

Chemotherapy-induced cognitive dysfunction (chemobrain) is an important adverse sequela of chemotherapy. Chemobrain has been identified by the National Cancer Institute as a poorly understood problem for which current management or treatment strategies are limited or ineffective. Here, we show that chemotherapy treatment with doxorubicin (DOX) in a breast cancer mouse model induced protein kinase A (PKA) phosphorylation of the neuronal ryanodine receptor/calcium (Ca2+) channel type 2 (RyR2), RyR2 oxidation, RyR2 nitrosylation, RyR2 calstabin2 depletion, and subsequent RyR2 Ca2+ leakiness. Chemotherapy was furthermore associated with abnormalities in brain glucose metabolism and neurocognitive dysfunction in breast cancer mice. RyR2 leakiness and cognitive dysfunction could be ameliorated by treatment with a small molecule Rycal drug (S107). Chemobrain was also found in noncancer mice treated with DOX or methotrexate and 5-fluorouracil and could be prevented by treatment with S107. Genetic ablation of the RyR2 PKA phosphorylation site (RyR2-S2808A) also prevented the development of chemobrain. Chemotherapy increased brain concentrations of the tumor necrosis factor-α and transforming growth factor-ß signaling, suggesting that increased inflammatory signaling might contribute to oxidation-driven biochemical remodeling of RyR2. Proteomics and Gene Ontology analysis indicated that the signaling downstream of chemotherapy-induced leaky RyR2 was linked to the dysregulation of synaptic structure-associated proteins that are involved in neurotransmission. Together, our study points to neuronal Ca2+ dyshomeostasis via leaky RyR2 channels as a potential mechanism contributing to chemobrain, warranting further translational studies.

Osteosarcoma in a free-living yellow armadillo (Euphractus sexcinctus).

An adult male free-living yellow armadillo (Euphractus sexcinctus) was found by hunters and referred for clinical evaluation because of a tumour-like lesion on the carapace. The animal was lethargic and weak with severe dehydration, enophthalmos and cachexia, and was euthanized because of its very poor clinical condition. Necropsy revealed a whitish, exophytic, irregular and moist mass (7 × 6.5 × 1.5 cm) in the caudal third of the carapace. On cut section, the mass was hard, compact, irregular and whitish. Histopathology revealed a densely cellular, expansive, poorly delimited neoplasm composed of malignant mesenchymal cells arranged in islands and cords, interspersed by numerous areas of irregularly mineralized osteoid matrix. Neoplastic cells were intensely immunolabelled for vimentin. The diagnosis of a high-grade osteosarcoma of osteoblastic subtype was based on the clinical, gross, histopathological and immunohistochemical findings. This first report of an osteosarcoma in an armadillo expands the list of armadillo diseases and will assist the management of these animals by veterinarians.

Upper and Lower Limbs Acute Fatigue Did Not Mitigate Male Trained Air Force Soldiers' Marksmanship.

In this study, we aimed to investigate the impact of acute fatigue on pistol shooting performance among Air Force marksmen. We compared the accuracy, precision, speed-accuracy trade-off, shooting cycle time, and hits on a silhouette target among 12 Brazilian Air Force servicemen (M age = 21.5, SD - 1.6 years) under both fatigue and non-fatigue conditions in a crossover design. In the fatigued condition, the participants performed a fatigue protocol composed of side runs, vertical jumps, push-ups, running, and burpees exercises before shooting. Participants performed the countermovement jump and the plyometric push-ups tests on a contact mat before and immediately after the fatigue protocol to compare the heights achieved pre- and post-fatigue. Paired t-tests showed a significant performance reduction of 34.36% and 40.02% for the countermovement jump and plyometric push-ups, respectively, indicating that participants were fatigued in their lower and upper limbs. In the non-fatigued condition, no exercise was performed before shooting. Results indicated no significant differences between conditions on shooting precision (p = .125; ES: .54), speed-accuracy trade-off (p = .261; ES = .33), hits within the silhouette (p = .167; ES = .41), or shooting cycle times (p = .868; ES = .05); but accuracy was greater (p = .025; ES: .54) when fatigued. We concluded that overall shooting performance was not impaired by physical fatigue, and shooting accuracy appeared to be improved. Perhaps physical fatigue was not enough to impair shooting accuracy in this young adult group, as accuracy decline is expected instead when shooters are in an exhausted state. Further research is needed to confirm these findings and test this presumption.

Adaptive Parameters for LoRa-Based Networks Physical-Layer.

Sub-GHz communication provides long-range coverage with low power consumption and reduced deployment cost. LoRa (Long-Range) has emerged, among existing LPWAN (Low Power Wide Area Networks) technologies, as a promising physical layer alternative to provide ubiquitous connectivity to outdoor IoT devices. LoRa modulation technology supports adapting transmissions based on parameters such as carrier frequency, channel bandwidth, spreading factor, and code rate. In this paper, we propose SlidingChange, a novel cognitive mechanism to support the dynamic analysis and adjustment of LoRa network performance parameters. The proposed mechanism uses a sliding window to smooth out short-term variations and reduce unnecessary network re-configurations. To validate our proposal, we conducted an experimental study to evaluate the performance concerning the Signal-to-Noise Ratio (SNR) parameter of our SlidingChange against InstantChange, an intuitive mechanism that considers immediate performance measurements (parameters) for re-configuring the network. The SlidingChange is compared with LR-ADR too, a state-of-the-art-related technique based on simple linear regression. The experimental results obtained from a testbed scenario demonstrated that the InstanChange mechanism improved the SNR by 4.6%. When using the SlidingChange mechanism, the SNR was around 37%, while the network reconfiguration rate was reduced by approximately 16%.

Tube-in-tube endoscopic vacuum therapy for treatment of colorectal anastomotic leaks: A low-cost, patient-friendly, feasible and efficient technical modification of sponge-based endoscopic vacuum therapy.

BACKGROUND AND AIMS: Colorectal endoscopic vacuum therapy (CR EVT) is usually performed using sponges passed through the anus. It may be associated with patient discomfort and displacement of the aspiration tube. METHODS: With the tube-in-tube endoscopic vacuum therapy modification (CR TT-EVT), it is possible to position the aspiration tube in the pelvic cavity through the abdominal wall. In addition, it allows frequent cleaning of the fistula, eliminates the need for programmed device changes, and enables a standardized approach to such a wide variety of fistulas, leaks, and perforations. RESULTS: Here is a technical note on how to perform CR TT-EVT, while we are at the early phase of our case series we have reached 100% of technical success.

Does Relative Age Effect Exist in Paralympic Sport? A Study With Brazilian Paralympic Swimmers.

A Relative Age Effect (RAE), by which young athletes with birthdates early in a calendar year have experienced a team selection advantage that persists throughout their careers, has been found to be prevalent in many sports. However, this phenomenon has not been investigated in the Paralympic sports context. Thus, we aimed to investigate the prevalence of RAE among male and female Brazilian Paralympic swimmers. Data from 694 ranked athletes were collected from the 2021 Brazilian Paralympic Swimmers National rankings. Athletes' birthdates were divided into four quarters (Q1, Q2, Q3, and Q4) according to their month of birth. Chi-Square (χ2) goodness-of-fit tests were used to compare the observed and expected distributions of athletes born in each quarter, based on sex (male and female), impairment type (physical, visual, and intellectual), and swim stroke competition (freestyle, medley, backstroke, butterfly, and breaststroke). The observed birthdates distributions were different from expected in males (χ2 = 11.647; p = 0.009) and females (χ2 = 8.899; p = 0.031), for athletes with physical impairments (χ2 = 10.443; p = 0.015); and for athletes who competed in freestyle (χ2 = 16.683; p = 0.001), medley (χ2 = 12.343; p = 0.006) and backstroke (χ2 = 8.025; p = 0.045) races. Even though our results demonstrated asymmetric distributions of Brazilian Paralympic swimmers' birthdates in many of the analyses, we could not establish the classical prevalence of athletes born at the beginning of the year that defines RAE. Therefore, the selection process of Brazilian Paralympic swimmers does not seem to be influenced by the athletes' time of birth.

Are relative age effects pervasive in lower competitive tiers of school sports? An investigation of student-athletes from Minas Gerais / Los efectos de la edad relativa son dominantes en los niveles menos competitivos del deporte escolar? Una investigación de estudiantes-deportistas de minas gerais / Os efeitos da idade relativa são pervasivos nos níveis menos competitivos do esporte escolar? Uma investigação de estudantes-atletas de Minas Gerais

Este estudio investigaba el Efecto de la Edad Relativa (EEA) en atletas escolares, según categorías y género. Han sido analizados 1356 jugadores masculinos y 1044 femeninas (de 9 a 17 años) que participaron en competencias escolares en Minas Gerais-Brasil en 2018 y/o 2019 en las categorías Sub-11, Sub-14 y/o Sub-17. Los jugadores fueron divididos en cuartos, de acuerdo con el mes de nacimiento: Q1 (enero-marzo), Q2 (abril-junio), Q3 (julio-septiembre) y Q4 (octubre-diciembre). Se realizaron pruebas de chi-cuadrado para verificar la presencia de EEA. El nivel de significación fue 5%. La distribución observada difirió de la esperada cuando todos los jugadores fueron analizados juntos (p = 0.019; ⍵ = 0.06), y en los hombres, cuando los jugadores fueron analizados por género (p = 0.017; ⍵ = 0.09). En ambos casos se encontró una mayor proporción de jugadores nacidos en los primeros meses del año (p < 0.008). Los resultados indicaron una distribución desigual de nacimientos en la categoría Sub-11 (p < 0.001; ⍵ = 0.25), en la que deportistas nacidos en Q1 y Q2 fueron más frecuentes (p < 0.001). Cuando se analizaron juntos categoría y género, se observó que atletas nacidos en Q1 fueron más frecuentes que los deportistas nacidos en Q3 (p < 0.002) y Q4 (p < 0.001) en la Sub-11, independientemente del género (p < 0.002), pero no en las categorías Sub-14 y Sub-17. Teniendo en cuenta que el deporte escolar puede ser el primer paso para alcanzar mayores niveles competitivos, es importante instruir a los entrenadores sobre el EEA, con el fin de reducir las desigualdades en el acceso al deporte escolar. (AU)

This study investigated the Relative Age Effect (RAE) in school athletes, according to sex and age categories. Birthdates from 1356 male and 1044 female athletes (aged 9 to 17 years) who played scholar competitions in Minas Gerais-Brazil in 2018 and/or 2019 in categories Under-11, Under-14 and/or Under-17 were analyzed. Athletes were divided into quarters according to their month of birth: Q1 (January-March), Q2 (April-June), Q3 (July-September), and Q4 (October-December). Chi-squared tests were performed to verify the presence of RAE. The significance level was set at 5%. The observed distribution was different from expected when all athletes were analyzed together (p = 0.019; ⍵= 0.06), and in males when athletes were analyzed separately by sex (p = 0.017; ⍵= 0.09). Athletes born in the three first months of the year were more frequent in both cases (p < 0.008). The age category analyses indicated uneven distribution of births in Under-11 category (p < 0.001; ⍵= 0.25), where athletes born in Q1 and Q2 were more frequent (p < 0.001). When age category and sex were considered together, it was observed that athletes born in Q1 were more frequent than athletes born in Q3 (p < 0.002) and Q4 (p < 0.001) in the U-11 category regardless of sex (p < 0.002), but not in the Under-14 and Under-17 categories. Considering that school sport can be the first step towards achieving higher competitive levels, it is important to instruct coaches about RAE, in order to reduce inequalities in the access to school sport. (AU)

Este estudo investigou o Efeito da Idade Relativa (EIR) em atletas escolares, de acordo com categorias e sexo. Analisaram-se datas de nascimento de 1356 atletas do sexo masculino e 1044 do sexo feminino (de 9 a 17 anos) que participaram de competições escolares em Minas Gerais-Brasil em 2018 e/ou 2019 nas categorias Sub-11, Sub-14 e/ou Sub-17. Os atletas foram divididos em quartis, de acordo com o mês de nascimento: Q1 (janeiro-março), Q2 (abril-junho), Q3 (julho-setembro) e Q4 (outubro-dezembro). Realizaram-se testes de chi-quadrado para verificar a presença do EIR. O nível de significância adotado foi de 5%. A distribuição observada diferiu do esperado quando todos os atletas foram analisados em conjunto (p = 0.019; ⍵= 0.06), e no sexo masculino, quando os atletas foram analisados por sexo (p = 0.017; ⍵= 0.09). Encontrou-se maior proporção de atletas nascidos nos primeiros meses do ano em ambos os casos (p < 0.008). Os resultados indicaram uma distribuição desigual de nascimentos na categoria Sub-11 (p < 0.001; ⍵= 0.25), onde atletas nascidos nos Q1 e Q2 foram mais frequentes (p < 0.001). Quando a categoria e o sexo foram analisados conjuntamente, se observou que atletas nascidos no Q1 foram mais frequentes que atletas nascidos em Q3 (p < 0.002) e Q4 (p < 0.001) no Sub-11, independentemente do sexo (p < 0.002), mas não nas categorias Sub-14 e Sub-17. Considerando que o esporte escolar pode ser o primeiro passo para alcançar maiores níveis competitivos, é importante instruir os treinadores sobre o EIR, a fim de reduzir as desigualdades de oportunidades de acesso ao esporte escolar. (AU)

Influência do efeito da idade relativa no futsal masculino escolar de Mato Grosso: análise de acordo com a categoria / Influence of the relative age effect on male school futsal in Mato Grosso: a category-based analysis / Influencia del efecto de la edad relativa en el fútbol sala escolar masculino de Mato Grosso: análisis de acuerdo con la categoría

O objetivo deste estudo foi analisar a existência e a influência do Efeito da Idade Relativa (EIR) em estudantes-atletas de futsal masculino de Mato Grosso de acordo com a categoria etária. A amostra foi composta por 486 estudantes-atletas masculinos de futsal de Mato Grosso das categorias A (15 a 17 anos) e B (12 a 14 anos) que disputaram os Jogos Escolares Mato-grossenses e os Jogos Estudantis de Seleções Mato-Grossenses de 2021. Para análise, foram realizados testes qui-quadrado (χ2) de aderência. Os resultados mostraram a presença do EIR na análise geral e nas categorias A e B, com maior representação de atletas nascidos no primeiro trimestre do ano. Conclui-se que o EIR é um fenômeno presente em estudantes-atletas de futsal masculino do estado de Mato Grosso.

The objective of this study was to analyze the existence and influence of the Relative Age Effect (RAE) in male Mato Grosso's futsal student-athletes according to age category. The sample consisted of 486 male futsal student-athletes from Mato Grosso, in categories A (15 to 17 years old) and B (12 to 14 years old) who competed in the 2021 Mato Grosso School Games and the Mato Grosso Student Selections Games. For analysis, chi-square (χ2) goodness of fit tests were performed. Results showed the presence of RAE in the overall analysis and in categories A and B, with a greater representation of athletes born in the first quarter of the year. It is concluded that RAE is a phenomenon present in male futsal student-athletes in the state of Mato Grosso.

El objetivo de este estudio fue analizar la existencia e influencia del Efecto de la Edad Relativa (EER) en estudiantes-atletas de fútbol sala masculino de Mato Grosso de acuerdo con la categoría de edad. La muestra estuvo compuesta por 486 estudiantes-atletas masculinos de fútbol sala de Mato Grosso de las categorías A (15 a 17 años) y B (12 a 14 años) que compitieron en los Juegos Escolares de Mato Grosso y los Juegos Estudiantiles de Selecciones de Mato-Grosso (Brasil) de 2021. Para el análisis, se realizaron pruebas estadísticas de ajuste chi-cuadrado (χ2). Los resultados mostraron la presencia del EER en el análisis general y en las categorías A y B, con una mayor representación de atletas nacidos en el primer trimestre del año. Se concluye que el EER es un fenómeno presente en estudiantes-atletas de fútbol sala masculino del estado de Mato Grosso.

The Relative Age Effect in Male and Female Brazilian Elite Volleyball Athletes of Varied Competitive Levels.

Volleyball is a popular sport in Brazil, and the relative age effect (RAE) is known to occur within it; but less is known of how RAE relates to elite Brazilian volleyball players' age, sex, and competitive levels. We aimed to investigate RAE prevalence with data from two seasons of play among players in the Superliga A (2020/2021 and 2021/2022 seasons), and Superliga B (2021 and 2022 seasons) made available from the Brazilian Volleyball Confederation (CBV), the club's official website, or direct consultation with the CBV. After removing duplicate data, we grouped these 1,063 athletes by their dates of birth, sex, and competition level (Superliga A or B). We divided players' birth dates into quarters (Q1: January-March, Q2: April-June, Q3: July-September and Q4: October-December) and into semesters, and we performed chi-square (χ2) tests to investigate RAE prevalence according to the players' sex and competitive level. RAE was prevalent overall (χ2 = 33.198; p < .001), among males (χ2 = 24.48; p < .001) and females (χ2 = 11.23; p < .011). Regarding competition level, RAE was evident among males in both Superliga A (χ2 = 14.581; p = 0.002), and B (χ2 = 13.985; p = 0.003), and among females in Superliga B (χ2 = 9.204; p = 0.027), but not Superliga A (χ2 = 4.012; p = 0.26). Thus, the RAE phenomenon operated differently for male and female Brazilian volleyball players according to their competitive level. We discuss the implications of these findings.

Expectation of Teaching and Self-Controlled KR in Motor Skills Acquisition: Are There Additive Effects?

Purpose: Recent evidence suggests learning a motor skill with the expectation of teaching it enhances motor learning. The mechanisms underlying this effect seem to be similar to those of another motor learning condition, the self-control of knowledge of results (KR). Considering the similarities between the mechanisms that underlie these conditions, we aimed to investigate the learning effects obtained through expected teaching and self-controlled conditions, and whether these effects would be additive. Methods: Participants practiced a dart-throwing task under one of the following conditions: a) expecting to teach the skill; b) controlling the KR request; c) combining the two previous conditions; and d) receiving KR in a yoked condition with self-controlled participants. In acquisition phase, motivational aspects, strategies for requesting KR and aspects related to the expectation of teaching were assessed according to each condition. Results: Participants with control over KR and/or with the expectation of teaching the skill showed superior learning of the task compared to the control condition. However, the combination of the experimental conditions did not result in additive learning benefits. Increased perceived competence was found in expecting to teach, self-controlled and combined conditions, compared to the yoked group. Additionally, expecting to teach also affected the way and the frequency learners requested KR. Conclusions: Our findings provide important insights toward understanding the effects of expecting to teach, in addition to demonstrating that expecting to teach affects self-controlled KR scheduling and its use during motor skill acquisition.

Prevalence of the Relative Age Effect in Elite Brazilian Volleyball: An Analysis Based on Gender, the Playing Position, and Performance Indicators.

Athletes born closer to an arbitrary cut-off date are more likely to reach an elite level in sport, which is supported by a phenomenon called the relative age effect (RAE). It is important to determine whether this phenomenon is present in a sport to minimize this selection bias. This study aimed to investigate the prevalence of RAE in elite volleyball athletes, considering the influence of gender, the playing position (Setter, Middle, Libero, Opposite, and Outside Hitter) and the performance level (attack points, aces, and block points). The sample comprised 203 male and 193 female athletes competing in the Superliga A in the 2020/2021 season, which was equivalent to all of the teams of the championship. The data collection was performed during May and June, 2021. Athletes were organized according to gender, the playing position, and performance in the Superliga. For performance variables, athletes were separated based on the median value (90.0), and classified as high- or low-performance. Chi-squared tests were performed to verify differences between birth date distributions in relation to the aforementioned variables. Results indicated overrepresentation of relatively older male athletes (Q1 = 35.96%; Q2 = 27.59%; Q3 = 19.21%; Q4 = 17.24%), especially in Middles, Opposites, and Outside Hitters, regardless of their performance level. Considering females, no differences were found. Our findings suggest that RAE operates differently for men and women in elite Brazilian volleyball. The characteristics of the games played by male and female elite athletes may lead to different talent selection processes, affecting the likelihood of RAE prevalence.

The 677C > T variant in methylenetetrahydrofolate reductase causes morphological and functional cerebrovascular deficits in mice.

Vascular contributions to cognitive impairment and dementia (VCID) particularly Alzheimer's disease and related dementias (ADRDs) are increasing; however, mechanisms driving cerebrovascular decline are poorly understood. Methylenetetrahydrofolate reductase (MTHFR) is a critical enzyme in the folate and methionine cycles. Variants in MTHFR, notably 677 C > T, are associated with dementias, but no mouse model existed to identify mechanisms by which MTHFR677C > T increases risk. Therefore, MODEL-AD created a novel knock-in (KI) strain carrying the Mthfr677C > T allele on the C57BL/6J background (Mthfr677C > T) to characterize morphology and function perturbed by the variant. Consistent with human clinical data, Mthfr677C > T mice have reduced enzyme activity in the liver and elevated plasma homocysteine levels. MTHFR enzyme activity is also reduced in the Mthfr677C > T brain. Mice showed reduced tissue perfusion in numerous brain regions by PET/CT as well as significantly reduced vascular density, pericyte number and increased GFAP-expressing astrocytes in frontal cortex. Electron microscopy revealed cerebrovascular damage including endothelial and pericyte apoptosis, reduced luminal size, and increased astrocyte and microglial presence in the microenvironment. Collectively, these data support a mechanism by which variations in MTHFR perturb cerebrovascular health laying the foundation to incorporate our new Mthfr677C > T mouse model in studies examining genetic susceptibility for cerebrovascular dysfunction in ADRDs.

Relative age effect on elite men’s futsal according to region and playing position:A study of the FIFA Futsal World Cup Lithuania 2021 / Efecto de la edad relativa en el fútbol sala masculino de elite según la región y la posición de juego: Unestudio de la Copa Mundial de Fútbol Sala FIFA Lituania 2021 / Efeito da idade relativa no futsal de elite masculino de acordo com a região e posição de jogo: Um estudo daCopa do Mundo de Futsal FIFA Lituânia 2021

Objective: To investigate the existence of relative age effect (RAE) in male futsal athletes that participated in the “FIFA Futsal World Cup Lithuania 2021”,according to region and playing position.Methods: The birthdates of 384 male futsal athletes participating in the competition were collected from official websites. Players were divided accordingto their continents and playing positions, and the chi-square goodness-of-fit tests ( 2) were performed to compare the expected and the observed birthχ2) were performed to compare the expected and the observed birth-date absolute distributions for each group. Also, specific effect sizes (⍵) were calculated for each of the chi-square tests performed. Additionally, OddsRatio (ORs) and 95% confidence intervals were calculated for the first and the last quarters of the year, as for semesters. Results: The overall analysis of the athletes indicated no presence of RAE in this sample, since an even distribution of birthdates was found ( 2 = 5.949; pχ2) were performed to compare the expected and the observed birth= 0.114; = 0.124). The regions analysed also indicated no presence of RAE. When playing positions were considered, once again analyses indicated noω = 0.124). The regions analysed also indicated no presence of RAE. When playing positions were considered, once again analyses indicated noRAE to elite international futsal athletes, since no differences were found between the observed and expected birthdate distributions.Conclusion: RAE is not prevalent on the international male elite futsal context. This effect was not associated with region or playing positions. From apractical perspective, it is important that selection processes consider the incidence of the RAE and give similar opportunities to athletes regardless oftheir birthdate.(AU)

Objetivo: Investigar la existencia del efecto de la edad relativa (EER) en atletas masculinos de fútbol sala que participaron en la “Copa Mundial de Fútbol Sala FIFA Lituania 2021”, según región y posición de juego.Métodos: Las fechas de nacimiento de 384 atletas masculinos de fútbol sala que participaron en la competencia fueron obtenidas de los sitios web oficiales. Se dividieron los jugadores de acuerdo con sus continentes y posiciones de juego, y se realizaron los testes de chi-cuadrado goodness-of-fit ( 2) χ2) were performed to compare the expected and the observed birthpara comparar las distribuciones absolutas de fecha de nacimiento esperada y observada para cada grupo. Así, se calcularon tamaños de efecto específicos ( ) para cada tests de chi-cuadrado Además, se calcularon Odds Ratio (OR) e intervalos de confianza del 95% para el primer y último ⍵trimestre del año, al igual que para los semestres. Resultados: El análisis general de los atletas indicó que no existe EER en esta muestra, ya que se encontró una distribución uniforme de las fechas de nacimiento ( 2 = 5.949; p = 0.114; = 0.124). Los análisis de regiones también no indicaron presencia de EER. Cuando se consideraron las posiciones deχ2) were performed to compare the expected and the observed birthω = 0.124). The regions analysed also indicated no presence of RAE. When playing positions were considered, once again analyses indicated nojuego, una vez más los análisis no indicaron EER para los atletas, ya que no se encontraron diferencias entre las distribuciones de fechas de nacimiento observadas y esperadas.Conclusiones: El EER no prevalece en el contexto internacional del fútbol sala de élite masculino. Este efecto no se asoció con la región o las posiciones de juego. En la práctica, es importante que en el proceso de selección considerérese la incidencia de EER y que hay oportunidades similares a los atletas independientemente de su fecha de nascimiento.(AU)

Objetivo: Investigar a existência de efeito de idade relativa (EIR) em atletas masculinos de futsal que participaram da “Copa do Mundo de Futsal FIFALituânia 2021”, de acordo com a região e posição de jogo.Métodos: As datas de nascimento de 384 atletas masculinos de futsal que participam da competição foram coletadas de sites oficiais. Os jogadores foramdivididos de acordo com seus continentes e posições de jogo, e os testes qui-quadrado de aderência ( 2) foram realizados para comparar as distribuiçõesχ2) were performed to compare the expected and the observed birthabsolutas esperadas e observadas de nascimento para cada grupo. Assim, foram calculados tamanhos de efeito específicos ( ) para cada um dos testes⍵qui-quadrado realizados. Adicionalmente, foram calculados Odds Ratio (ORs) e intervalos de confiança de 95% para o primeiro e último trimestres doano, assim como para os semestres.Resultados: A análise geral dos atletas não indicou a presença de EIR nesta amostra, uma vez que foi encontrada uma distribuição uniforme das datas denascimento ( 2 = 5.949; p = 0.114; = 0.124). As análises das regiões também não indicaram a presença de EIR. Ao considerar as posições de jogo, maisχ2) were performed to compare the expected and the observed birthω = 0.124). The regions analysed also indicated no presence of RAE. When playing positions were considered, once again analyses indicated nouma vez as análises não indicaram EIR para atletas de elite do futsal internacional, uma vez que não foram encontradas diferenças entre as distribuiçõesde datas de nascimento observadas e esperadas.Conclusões: O EIR não é prevalente no contexto internacional de futsal de elite masculino. Este efeito não foi associado à região ou às posições de jogo. Naprática, é importante que o processo de seleção de talentos considere a incidência do EIR e forneça oportunidades similares aos atletasindependentemente da sua data de nascimento.(AU)

Pharmacokinetic, pharmacodynamic, and transcriptomic analysis of chronic levetiracetam treatment in 5XFAD mice: A MODEL-AD preclinical testing core study.

Introduction: Hyperexcitability and epileptiform activity are commonplace in Alzheimer's disease (AD) patients and associated with impaired cognitive function. The anti-seizure drug levetiracetam (LEV) is currently being evaluated in clinical trials for ability to reduce epileptiform activity and improve cognitive function in AD. The purpose of our studies was to establish a pharmacokinetic/pharmacodynamic (PK/PD) relationship with LEV in an amyloidogenic mouse model of AD to enable predictive preclinical to clinical translation, using the rigorous preclinical testing pipeline of the Model Organism Development and Evaluation for Late-Onset Alzheimer's Disease Preclinical Testing Core. Methods: A multi-tier approach was applied that included quality assurance and quality control of the active pharmaceutical ingredient, PK/PD modeling, positron emission tomography/magnetic resonance imaging (PET/MRI), functional outcomes, and transcriptomics. 5XFAD mice were treated chronically with LEV for 3 months at doses in line with those allometrically scaled to the clinical dose range. Results: Pharmacokinetics of LEV demonstrated sex differences in Cmax, AUC0-∞, and CL/F, and a dose dependence in AUC0-∞. After chronic dosing at 10, 30, 56 mg/kg, PET/MRI tracer 18F-AV45, and 18F-fluorodeoxyglucose (18F-FDG) showed specific regional differences with treatment. LEV did not significantly improve cognitive outcomes. Transcriptomics performed by nanoString demonstrated drug- and dose-related changes in gene expression relevant to human brain regions and pathways congruent with changes in 18F-FDG uptake. Discussion: This study represents the first report of PK/PD assessment of LEV in 5XFAD mice. Overall, these results highlighted non-linear kinetics based on dose and sex. Plasma concentrations of the 10 mg/kg dose in 5XFAD overlapped with human plasma concentrations used for studies of mild cognitive impairment, while the 30 and 56 mg/kg doses were reflective of doses used to treat seizure activity. Post-treatment gene expression analysis demonstrated LEV dose-related changes in immune function and neuronal-signaling pathways relevant to human AD, and aligned with regional 18F-FDG uptake. Overall, this study highlights the importance of PK/PD relationships in preclinical studies to inform clinical study design. Highlights: Significant sex differences in pharmacokinetics of levetiracetam were observed in 5XFAD mice.Plasma concentrations of 10 mg/kg levetiracetam dose in 5XFAD overlapped with human plasma concentration used in the clinic.Drug- and dose-related differences in gene expression relevant to human brain regions and pathways were also similar to brain region-specific changes in 18F-fluorodeoxyglucose uptake.

Plcg2M28L Interacts With High Fat/High Sugar Diet to Accelerate Alzheimer's Disease-Relevant Phenotypes in Mice.

Obesity is recognized as a significant risk factor for Alzheimer's disease (AD). Studies have supported the notion that obesity accelerates AD-related pathophysiology in mouse models of AD. The majority of studies, to date, have focused on the use of early-onset AD models. Here, we evaluate the impact of genetic risk factors on late-onset AD (LOAD) in mice fed with a high fat/high sugar diet (HFD). We focused on three mouse models created through the IU/JAX/PITT MODEL-AD Center. These included a combined risk model with APOE4 and a variant in triggering receptor expressed on myeloid cells 2 (Trem2R47H ). We have termed this model, LOAD1. Additional variants including the M28L variant in phospholipase C Gamma 2 (Plcg2M28L ) and the 677C > T variant in methylenetetrahydrofolate reductase (Mthfr 677C > T ) were engineered by CRISPR onto LOAD1 to generate LOAD1.Plcg2M28L and LOAD1.Mthfr 677C > T . At 2 months of age, animals were placed on an HFD that induces obesity or a control diet (CD), until 12 months of age. Throughout the study, blood was collected to assess the levels of cholesterol and glucose. Positron emission tomography/computed tomography (PET/CT) was completed prior to sacrifice to image for glucose utilization and brain perfusion. After the completion of the study, blood and brains were collected for analysis. As expected, animals fed a HFD, showed a significant increase in body weight compared to those fed a CD. Glucose increased as a function of HFD in females only with cholesterol increasing in both sexes. Interestingly, LOAD1.Plcg2M28L demonstrated an increase in microglia density and alterations in regional brain glucose and perfusion on HFD. These changes were not observed in LOAD1 or LOAD1.Mthfr 677C > T animals fed with HFD. Furthermore, LOAD1.Plcg2M28L but not LOAD1.Mthfr 677C > T or LOAD1 animals showed transcriptomics correlations with human AD modules. Our results show that HFD affects the brain in a genotype-specific manner. Further insight into this process may have significant implications for the development of lifestyle interventions for the treatment of AD.

Did the Absence of Crowd Support During the Covid-19 Pandemic Affect the Home Advantage in Brazilian Elite Soccer?

This study aimed to investigate the impact of crowd absence due to the COVID-19 pandemic on home advantage in Series A and B of professional Brazilian male soccer. Moreover, we sought to compare the home advantage between different competitive levels. Data from 2018 to 2020 Brazilian professional soccer championships (Series A and B) were analyzed, consisting of 2280 matches. The effect of home advantage was calculated in relation to the number of points won, the number of wins, goals scored and goals conceded. Our results indicated that home advantage was reduced in Serie A. Specifically, the 2020 (absence of the crowd) and 2019 seasons showed smaller home advantage compared to the 2018 season. On the other hand, Serie B analyses indicated no changes in home advantage over the seasons analyzed. The comparison of home advantage between competitive levels indicated higher home advantage in Serie A, exclusively in the 2018 season. In addition to the absence of fans, other changes occurred in elite sports due to the pandemic, such as changes in rules and the calendar. Considering that home advantage is affected by multiple interacting factors, it is important to investigate specific sports leagues across the world to determine which factors had the greatest impact on the advantage of playing home matches.

Relative Age Effect in Brazilian water polo: analysis of male and female athletes from U16 to senior categories

Abstract Aim: To investigate the existence of relative age effect (RAE) in Brazilian water polo athletes according to sex and age category. Methods: The birthdate of 574 Brazilian water polo athletes were organized according to the athletes' birthdates into quarters of the year (Q1, Q2, Q3, and Q4), and classified according to sex (male and female) and age category (U16, U18, U20, and senior). To verify the existence of RAE, the Chi-Square tests (χ2) were performed, and the effect sizes (ω) were calculated for each of the tests. We also calculated odds ratio (ORs) and 95% confidence intervals, setting the level of significance to 0.05. As post hoc analysis, multiple comparisons between quarters were performed, with Bonferroni's correction (significance level set to 0.0083 in these cases). Results: The results indicated an uneven distribution of birthdates for male water polo athletes (χ2 = 12.257; p = 0.007; ω = 0.173), with an overrepresentation of athletes born in the first (p < 0.006) and second (p < 0.002) quarters. When sex and age category were considered, male athletes presented uneven distributions in U20 (χ2 = 10.747; p = 0.013; ω = 0.345) and senior (χ2= 12.614; p = 0.006; ω = 0.383) categories. In females, no differences were found. Conclusion: We conclude that there is an uneven distribution of birthdates in male Brazilian water polo athletes, indicating the presence of RAE in this group.