Absence of correlation between IL-28B gene polymorphisms and the clinical presentation of chronic hepatitis B in an Amazon Brazilian population.

OBJECTIVE: The present study investigated the prevalence of the IL-28B polymorphisms rs12979860 and rs8099917 in chronic hepatitis B patients from a case study in Eastern Amazonia. METHODS: In total, 65 chronically infected HBV patients and 97 healthy subjects who were anti-HBc and anti-HBs positive (control group) were evaluated between May 2011 and December 2012. The groups of patients were designated as inactive carriers, chronic hepatitis without cirrhosis, and chronic hepatitis with cirrhosis based on clinical, pathological, biochemical, hematological, and virological variables. The patients were genotyped using quantitative real-time PCR. RESULTS: The frequencies of the rs12979860 polymorphism were similar between the infected group (32.3% CC, 41.5% CT, and 26.2 TT) and the control population (35% CC, 47.4% CT, and 17.6% TT), and the frequencies of the rs8099917 polymorphism (7.7% GG, 35.4% GT, and 56.9% TT versus 7.2% GG, 35.1% GT, and 57.7% TT) were also similar in both groups. The associations between the rs12979860 and rs8099917 polymorphisms and the clinical manifestations were not statistically significant. CONCLUSION: In conclusion, these polymorphisms had a similar distribution between infected and control groups, indicating that they were not associated with susceptibility and the clinical evolution of hepatitis B in the examined population.

Efeito do extrato alcoólico da aloe vera na sobrevida de camundongos e no crescimento do tumor sólido de Ehrlich / Effect of alcoholic extract of aloe vera on survival of mice and tumor growth of solid ehrlich tumor

Objetivo: analisar o efeito do extrato alcoólicoda Aloe vera na sobrevida de camundongos e nocrescimento do tumor sólido de Ehrlich. Método: a amostra contou com 23 animais distribuídos em 04grupos com 05 animais em cada, sendo estes: Tumor + Água 1 (TA1), no qual os animais receberam águadestilada; Tumor + Aloe vera 1 (TAV1), no qual os animais receberam extrato alcóolico da Aloe vera, paraanálise da evolução tumoral. Para o estudo da sobrevida os grupos foram: Tumor + Água 2 (TA2) e Tumor+ Aloe Vera 2 (TAV2). O tumor foi injetado via subcutânea no dorso de todos os animais. A administraçãodas substâncias foi realizada por gavagem. No dia previsto, foi realizada eutanásia dos animais e adissecção tumoral, sendo as peças tumorais pesadas e realizada análise macroscópica e histopatológica.Resultados: no grupo TA1, a média do peso dos tumores ressecados foi 0,182g, detectou-se ascite eaderências em 02 animais e metástases em 04 destes. Já no grupo TAV2, a média do peso tumoral foi0,258g, detectou-se ascite e aderências em 01 animal e metástases foram verificadas em 04. Nos grupos deanálise da sobrevida, todos os animais do grupo TA2 conseguiram sobreviver até o 20o dia pós-implante, omesmo ocorreu somente com 03 animais do grupo TAV2. Conclusão: a utilização do extrato alcoólicodaAloe vera não apresentou efeito benéfico na sobrevida de camundongos e não se mostrou estatisticamenterelevante no crescimento do tumor sólido de Ehrlich.

Objective: to analyze the effect of alcoholic extract of Aloe veraon the survival of mice and growth ofEhrlich solid tumor. Methods: the sample consisted of 23 animals distributed in 04 groups with 05animals each, the groups were: Tumor + Water (TA1), in which the animals received distilled water;Tumor + Aloe vera 1 (TAV1), in which the animals received alcoholic extract of Aloe vera for analysis oftumor evolution. To study the survival rate the groups were: Tumor + Water 2 (TA2) and Tumor + AloeVera 2 (TAV2). The tumor was injected subcutaneously on the back of all animals. The administration ofthe substances was performed by gavage. Euthanasia and tumoral dissection were performed in thepredicted day, and the tumor pieces were weighed and a macroscopic and histopathological analysis wasperformed. Results: in TA1 group, the average weight of resected tumors was 0.182 g, and ascites andadhesions were detected in 02 animals and metastases in 04. In the group TAV2, the average tumorweight was 0.258 g, ascites and adhesions were detected in 01 animal and metastases in 04. In the groupsof survival analysis, all animals in group TA2 survived until the 20th day post-implant, the same occurredwith only 03 animals in the group TAV2. Conclusion: the use of alcoholic extract of Aloe vera showedno beneficial effect on survival of mice and was not statistically significant in the growth of Ehrlich solidtumor