RESUMO
BACKGROUND: Crop yields have been affected by many different biotic and abiotic factors. Generally, plants experience more than one stress during their life cycle, and plants can tolerate multiple stresses and develop cross-tolerance. The expected rise in atmospheric CO2 concentration ([CO2]) can contribute to cross-tolerance. Priming is a strategy to increase yield or to maintain yield under stress conditions. Thus, our objective was to evaluate if priming the rice plants with water deficit during the vegetative stage can induce tolerance to heat stress at anthesis and to evaluate the contribution of e[CO2]. METHODS: The experiment was arranged in a completely randomized design in a factorial arrangement. Factor A consisted of the following treatments: water deficit at four-leaf stage (no-stress, and drought stress), heat at anthesis (normal temperature, high temperature), and priming with water deficit at four-leaf stage and heat stress at anthesis; and Factor B was two [CO2] treatments: a[CO2] = 400 ± 40 µmol mol-1 and e[CO2] = 700 ± 40 µmol mol-1. We assessed the effect of the treatments on plant growth, yield, biochemical, and transcriptome alterations. RESULTS: Although e[CO2] affected rice growth parameters, it did not affect the priming effect. Primed plants showed an increase in yield and number of panicles per plant. Primed plants showed upregulation of OsHSP16.9A, OsHSP70.1, and OsHSP70.6. These results showed induced cross-tolerance. CONCLUSIONS: Water deficit at the rice vegetative stage reduces the effect of heat stress at the reproductive stage. Water deficit at the vegetative stage can be used, after further testing in field conditions, to reduce the effect of heat stress during flowering in rice.
RESUMO
The differential growth and yield response of plant species to rising carbon dioxide concentrations and climatic change may alter species diversity within biomes. The Pampa Biome in South America is an important grassland biome of agronomic and environmental importance. Acanthostyles buniifolius (Chirca) is one of the most important weeds in natural pasture areas widely distributed in southern South America and can adversely affect livestock production. The current study was designed to identify possible responses of Chirca to CO2 concentration ([CO2]) and drought that would indicate higher adaptation and potential proliferation within the Pampa Biome. Chirca plants were cultivated at two CO2 concentrations (400 (a[CO2]) and 700 (e[CO2]) µmol mol-1) and two water conditions (under water restriction-15% of the pot capacity; and plants without water restriction-pot capacity). Besides growth parameters, we also determined water potential (ѱw), relative water contents (RWC), proline, glycine betaine, total soluble sugars, hydrogen peroxide, lipid peroxidation, superoxide dismutase (SOD), ascorbate peroxidase (APX) activity, chlorophyll A and B, carotenoids and root dry mass (RDM). Plants exposed to e[CO2] are more efficient in water use and have a greater increase in root dry mass, enabling greater adaptation to climate-induced droughts. Among the biochemical changes observed in the plants under drought stress, the accumulation of proline, glycine betaine, and total soluble sugars were the most evident mechanisms allowing plants to tolerate drought stress by osmotic adjustment.
RESUMO
ABSTRACT: The use of imidazolinone-tolerant rice cultivars allows selective control of weedy rice and barnyardgrass. However, in many situations, there is a need to add herbicides from other chemical groups to increase the spectrum of weed control. In this sense, saflufenacil has the potential to be used in mixture with imidazolinone herbicides. This study aimed to evaluate the interaction effects of the imazapyr+imazapic and saflufenacil herbicides in weedy rice and barnyardgrass and to investigate their impacts on the yield of the irrigated rice cultivar Puitá INTA CL. To reach these aims, greenhouse and field experiments were carried out during two growing seasons, with herbicide treatments sprayed separately and in mixtures of saflufenacil with imazapyr+imazapic. Results showed that saflufenacil did not interfere with control of weedy rice and barnyardgrass obtained with imazapyr+imazapic. The D50 values of imazapyr+imazapic for weedy rice control were 14.5+5, 9.1+3 and 12.5+4.2 g ha-1of imazapyr+imazapic for combinations with 0, 3.06 and 6.12 g ha-1of saflufenacil, respectively. In the field experiments, all doses of imazapyr+imazapic applied isolated or in mixture with saflufenacil provided control levels barnyardgrass above 90% at 28 days after herbicides application. Furthermore, saflufenacil did not cause damage or loss in the yield of the rice crop when mixed with imazapyr+imazapic. The Puitá INTA CL rice cultivar was tolerant of the tested herbicides, whether applied alone or in mixture, reaching grain yield of 9.987 kg ha-1 when applied 42 g ha-1 of saflufenacil plus 147+49 g ha-1 of imazapyr+imazapic.
RESUMO: A utilização de cultivares de arroz tolerantes às imidazolinonas possibilita um controle seletivo de arroz-daninho. No entanto, em muitas situações existe a necessidade de adição de herbicidas de outros grupos químicos para aumentar o espectro de controle de plantas daninhas. Neste sentido, saflufenacil apresenta potencial para ser utilizado em mistura com herbicidas imidazolinonas. Este estudo teve como objetivo avaliar os efeitos da interação dos herbicidas imazapyr+imazapic e saflufenacil em arroz-daninho e capim-arroz; e averiguar os impactos sobre a produtividade do cultivar de arroz irrigado Puitá INTA CL. Para alcançar esses objetivos, foram realizados experimentos em casa de vegetação e a campo em duas estações de cultivo, com os tratamentos herbicidas aplicados separadamente e em misturas de saflufenacil com imazapyr+imazapic. Os resultados mostraram que a adição de saflufenacil não interferiu no controle de arroz-daninho e capim-arroz obtido com imazapyr+imazapic. Os valores D50 de imazapyr+imazapic para o controle de arroz-daninho foram 14,5+5; 9,1+3 e 12,5+4,2 g ha-1 de imazapyr+imazapic para combinações com 0; 3,06 e 6,12 g ha-1 de saflufenacil. No experimento de campo todas doses de imazapyr+imazapic, aplicadas isoladas ou em mistura, proveram níveis de controle de capim-arroz superiores a 90% aos 28 dias após a aplicação dos herbicidas. Além disso, saflufenacil não causou injúrias ou perda de rendimento da cultura do arroz quando misturado com imazapyr+imazapic. O cultivar Puitá INTA CL foi tolerante aos herbicidas testados, sejam aplicados isolados ou em mistura, atingindo uma produtividade de 9.987 Kg ha-1 quando aplicado 42 g ha-1 de saflufenacil mais 147+49 g ha-1 de imazapyr+imazapic.
