Improving Optical Flow Sensor Using a Gimbal for Quadrotor Navigation in GPS-Denied Environment.

This paper proposes a new sensor using optical flow to stabilize a quadrotor when a GPS signal is not available. Normally, optical flow varies with the attitude of the aerial vehicle. This produces positive feedback on the attitude control that destabilizes the orientation of the vehicle. To avoid this, we propose a novel sensor using an optical flow camera with a 6DoF IMU (Inertial Measurement Unit) mounted on a two-axis anti-shake stabilizer mobile aerial gimbal. We also propose a robust algorithm based on Sliding Mode Control for stabilizing the optical flow sensor downwards independently of the aerial vehicle attitude. This method improves the estimation of the position and velocity of the quadrotor. We present experimental results to show the performance of the proposed sensor and algorithms.

Genotyping of Toxoplasma gondii in domestic animals from Campeche, México, reveals virulent genotypes and a recombinant ROP5 allele.

Toxoplasma gondii has at least 318 genotypes distributed worldwide, and tropical regions usually have greater genetic diversity. Campeche is a state located in the southeastern region of México and has favourable climate conditions for the replication and dissemination of this protozoan, similar to those in South American countries where broad genetic diversity has been described. Thus, in this study, 4 T. gondii isolates were obtained from tissues of stray dogs and free-range chickens in Campeche, México, and were genotyped by Mn-PCR-RFLP with 10 typing markers (SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico) and 5 virulence markers (CS3, ROP16, ROP17, ROP18 and ROP5) to provide new information about the distribution and virulence prediction of T. gondii genotypes. Two isolates of T. gondii genotype #116 and 2 of genotype #38 were obtained from stray dogs and chickens, respectively. The parasite load found in these species was between <50 and more than 35 000 tachyzoites per mg of tissue. Virulence marker genotyping revealed a recombinant 1&3 ROP5 RFLP pattern in 2 ToxoDB #116 isolates with no prediction of virulence in a murine model, while in the 2 ToxoDB #38 isolates, the ROP18/ROP5 combination predicted high virulence. Considering all the typed markers, there is a predominance of type I and III alleles, as constantly reported for the isolates characterized in various regions of México. It is crucial to determine their phenotype to corroborate the genetic virulence profile of the T. gondii isolates obtained in this study.

Early Life Stress Negatively Impacts Spatial Learning Acquisition and Increases Hippocampal CA1 Microglial Activation After a Mild Traumatic Brain Injury in Adult Male Rats.

Early life stress (ELS) affects neurogenesis and spatial learning, and increases neuroinflammation after a pediatric mild traumatic brain injury (mTBI). Previous studies have shown that ELS has minimal effects in juveniles but shows age-dependent effects in adults. Hence, we aimed to evaluate the effects of ELS in adult male rats after an mTBI. Maternal separation for 180 min per day (MS180) during the first 21 post-natal (P) days was used as the ELS model. At P110, the rats were subjected to a mild controlled cortical impact injury (2.6 mm) or sham surgery. Spatial learning was evaluated in the Morris water maze (MWM) 14 days after surgery and both microglial activation and neurogenesis were quantified. The results indicate that MS180 + mTBI, but not control (CONT) + mTBI, rats show deficiencies in the acquisition of spatial learning. mTBI led to comparable increases in microglial activation in both the hilus and cortical regions for both groups. However, MS180 + mTBI rats exhibited a greater increase in microglial activation in the ipsilateral CA1 hippocampus subfield compared with CONT + mTBI. Interestingly, for the contralateral CA1 region, this effect was observed exclusively in MS180 + mTBI. ELS and mTBI independently caused a decrease in hippocampal neurogenesis and this effect was not increased further in MS180 + mTBI rats. The findings demonstrate that ELS and mTBI synergistically affect cognitive performance and neuroinflammation, thus supporting the hypothesis that increased inflammation resulting from the combination of ELS and mTBI could underlie the observed effects on learning.

Real-Time Person Detection in Wooded Areas Using Thermal Images from an Aerial Perspective.

Detecting people in images and videos captured from an aerial platform in wooded areas for search and rescue operations is a current problem. Detection is difficult due to the relatively small dimensions of the person captured by the sensor in relation to the environment. The environment can generate occlusion, complicating the timely detection of people. There are currently numerous RGB image datasets available that are used for person detection tasks in urban and wooded areas and consider the general characteristics of a person, like size, shape, and height, without considering the occlusion of the object of interest. The present research work focuses on developing a thermal image dataset, which considers the occlusion situation to develop CNN convolutional deep learning models to perform detection tasks in real-time from an aerial perspective using altitude control in a quadcopter prototype. Extended models are proposed considering the occlusion of the person, in conjunction with a thermal sensor, which allows for highlighting the desired characteristics of the occluded person.

Systemic lupus erythematosus in pediatric patients: Pulmonary manifestations.

The pulmonary manifestations of Systemic Lupus Erythematosus (SLE) in pediatric patients are poorly understood and the pulmonary manifestations reported from the adult population are generally extrapolated to the pediatric population. In the present work, the review of 228 files was carried out, in which the pulmonary manifestations, symptoms and antibody levels of the patients treated at the Hospital Regional de Alta Especialidad de Ixtapaluca (HRAEI), State of Mexico, Mexico, were identified. Statistical significance between groups was estimated using the Chi-square and Mann-Whitney U test. The main pulmonary manifestations identified were pleurisy (14 %), pulmonary hemorrhage (3.9 %), pulmonary thromboembolism (0.9 %), acute lupus pneumonitis (0.4 %), pulmonary arterial hypertension (0.4 %), and small lung syndrome (0.4 %). While the initial symptomatology was dyspnea with an incidence of 9.6 %, the mean oxygen saturation in the population was 96.87 %. Pleural effusion was identified as the most frequent pulmonary manifestation in radiographic changes. No statistically significant difference was found in antibody levels when comparing the groups. The most common pulmonary manifestation associated with SLE is pleurisy, however, the range of pulmonary manifestations in this type of patient can be very varied, as well as the presentation of each of them.

Unmanned aerial vehicle images in the machine learning for agave detection.

In this study, six supervised classification algorithms were compared. The algorithms were based on cluster analysis, distance, deep learning, and object-based image analysis. Our objective was to determine which of these algorithms has the highest overall accuracy in both detection and automated estimation of agave cover in a given area to help growers manage their plantations. An orthomosaic with a spatial resolution of 2.5 cm was derived from 300 images obtained with a DJI Inspire 1 unmanned aerial system. Two training classes were defined: (1) sites where the presence of agaves was identified and (2) "absence" where there were no agaves but other plants were present. The object-oriented algorithm was found to have the highest overall accuracy (0.963), followed by the support-vector machine with 0.928 accuracy and the neural network with 0.914. The algorithms with statistical criteria for classification were the least accurate: Mahalanobis distance = 0.752 accuracy and minimum distance = 0.421. We further recommend that the object-oriented algorithm be used, because in addition to having the highest overall accuracy for the image segmentation process, it yields parameters that are useful for estimating the coverage area, size, and shapes, which can aid in better selection of agave individuals for harvest.

Optical Identification of the Resonance-Stabilized para-Ethynylbenzyl Radical.

We report the spectroscopic observation of the jet-cooled para-ethynylbenzyl (PEB) radical, a resonance-stabilized isomer of C9H7. The radical was produced in a discharge of p-ethynyltoluene diluted in argon and probed by resonant two-color two-photon ionization (R2C2PI) spectroscopy. The origin of the D0(2B1)-D1(2B1) transition of PEB appears at 19,506 cm-1. A resonant two-color ion-yield scan reveals an adiabatic ionization energy (AIE) of 7.177(1) eV, which is almost symmetrically bracketed by CBS-QB3 and B3LYP/6-311G++(d,p) calculations. The electronic spectrum exhibits pervasive Fermi resonances, in that most a1 fundamentals are accompanied by similarly intense overtones or combination bands of non-totally symmetric modes that would carry little intensity in the harmonic approximation. Under the same experimental conditions, the m/z = 115 R2C2PI spectrum of the p-ethynyltoluene discharge also exhibits contributions from the m-ethynylbenzyl and 1-phenylpropargyl radicals. The former, like PEB, is observed herein for the first time, and its identity is confirmed by measurement and calculation of its AIE and D0-D1 origin transition energy; the latter is identified by comparison with its known electronic spectrum (J. Am. Chem. Soc., 2008, 130, 3137-3142). Both species are found to co-exist with PEB at levels vastly greater than might be explained by any precursor sample impurity, implying that interconversion of ethynylbenzyl motifs is feasible in energetic environments such as plasmas and flames, wherein resonance-stabilized radicals are persistent.

Electronic Spectroscopy of cis- and trans-meta-Vinylbenzyl Radicals.

The D0(2A″)-D1(2A″) electronic transition of resonance-stabilized radical C9H9 isomers cis- and trans-meta-vinylbenzyl (MVB) has been investigated using resonant two-color two-photon ionization (R2C2PI) and laser-induced fluorescence. The radicals were produced in a discharge of m-vinyltoluene diluted in Ar and probed under jet-cooled conditions. The origin bands of the cis and trans conformers are at 19 037 and 18 939 cm-1, respectively. Adiabatic ionization energies near 7.17 eV were determined for both conformers from two-color ion-yield scans. Dispersed fluorescence (DF) was used to conclusively identify the cis-conformer: ground-state cis-MVB eigenvalues calculated for a Fourier series fit of a computed vinyl torsion potential are in excellent agreement with torsional transitions in the 19 037 cm-1 DF spectrum. R2C2PI features arising from cis- or trans-MVB were distinguished by optical-optical hole-burning spectroscopy and vibronic assignments were made with guidance from density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations. There is a notable absence of mirror symmetry between excitation and emission spectra for several totally symmetric modes, whereby modes that are conspicuous in emission are nearly absent in excitation, and vice versa. This effect is largely ascribed to interference between Franck-Condon and Herzberg-Teller contributions to the electronic transition moment, and its pervasiveness a consequence of the low symmetry (Cs) of the molecule, which permits intensity borrowing from several relatively bright electronic states of A″ symmetry.

Tapirus bairdii-Associated Fecal Microbiome from a Critical Conservation Area: Calakmul, México.

Baird's tapir (Tapirus bairdii) is the largest native terrestrial mammal in the Neotropics, which is endangered primarily as a consequence of habitat loss and overhunting. Baird's tapir is predominantly nocturnal and exists at low densities which complicates field studies. Baird's tapir is a large-bodied herbivore that plays a key role in maintaining healthy tropical forests through seed dispersal in its feces. Studies of gut microbiome are essential and valuable to assess the health status of the host and the interaction with the environment. In this study, we collected fresh fecal samples of T. bairdii to analyze its gut microbiome during the rainy and dry seasons in the Calakmul region, which is a critical rainforest conservation area in Mexico. The results of a high-throughput 16S rDNA gene sequencing approach suggest that the fecal microbiome of Baird's tapir has no significant differences in composition among seasons. The most common phyla were Firmicutes, Bacteroidetes, Proteobacteria, Kiritimatiellaeota, and Spirochaetes. This study suggests that the stability of the fecal microbiome is related to similar feeding strategies throughout the year, and emphasizes the value of tapir in seed dispersal (and associated microbes) to the well-conserved forests of the Greater Calakmul region as biodiversity hotspots for conservation.

Heart urchins from the depths: Corparva lyrida gen. et sp. nov. (Palaeotropidae), and new records for the southwestern Atlantic Ocean / Erizos corazón de las profundidades: Corparva lyrida gen. et sp. nov. (Palaeotropidae) y nuevos registros para el Océano Atlántico sudoccidental

Introduction: Sea urchins in the order Spatangoida are the most diverse group of extant echinoids. Objective: Describe a new genus and species of Spatangoida from abyssal depths, and add new records for known species. Methods: Specimens were collected during several cruises at different areas of the southwestern Atlantic Ocean (SWAO), among 37-55° S latitude at depths ranging from 55 to 3 000 m. We present morphological and ultrastructure analyses. Results: Corparva lyrida gen. et sp. nov. (Palaeotropidae) is described from the Mar del Plata Canyon on the Argentine continental slope (2 950 m depth), the first record of this family from Argentina. Corparva gen. nov. differs in having an apical system semi-ethmolytic, and labrum reaching to rear part of second adjacent ambulacral plate. We also report the northernmost distribution and deepest record for Brisaster moseleyi (38° S latitude, 2 212 m depth), the northward extension of the distribution range of Tripylus excavatus (39° S latitude, 74 m depth), and the first record of Abatus philippii and Abatus agassizii at the Burdwood Bank/MPA Namuncurá. Conclusions: The present work brings novel and updated data about the diversity and distribution of spatangoids from the SWAO, including the description of C. lyrida gen. et sp. nov., and new records of species. This shows how much remains to be known about the diversity and distribution of heart urchins in the SWAO, especially from the deep-sea.

Introducción: Los erizos de mar del orden Spatangoida son el grupo más diverso de equinoideos recientes. Objetivo: Describir un nuevo género y una nueva especie de Spatangoida de profundidades abisales, y reportar nuevos registros para especies conocidas. Métodos: Los ejemplares fueron recolectados durante varias expediciones a diferentes áreas del Océano Atlántico sudoccidental (OAS), entre las latitudes 37-55° S y abarcando profundidades desde 55 a 3 000 metros. Presentamos análisis morfológicos y de ultraestructura. Resultados: Corparva lyrida gen. et sp. nov. (Palaeotropidae) fue descripta para el cañón submarino Mar del Plata en el talud continental de Argentina (2 950 m de profundidad), el primer registro de esta familia para Argentina. Corparva gen. nov. difiere en tener un sistema apical semi-etmolítico y labrum que llega a la parte posterior de la segunda placa ambulacral adyacente. También informamos la distribución más septentrional y el registro más profundo para Brisaster moseleyi (latitud 38° S, 2 212 m de profundidad), la extensión hacia el norte del rango de distribución de Tripylus excavatus (latitud 39° S, 74 m de profundidad) y el primer registro de Abatus philippii y Abatus agassizii en el Banco Burdwood/AMP Namuncurá. Conclusiones: El presente trabajo aporta datos novedosos y actualizados sobre la diversidad y distribución de erizos de mar espatangoideos del OAS, incluyendo la descripción de C. lyrida gen. et sp. nov., y nuevos registros para especies conocidas. Esto muestra cuánto queda por conocer sobre la diversidad y distribución de los erizos corazón en el OAS, especialmente de las profundidades marinas.

Environmental Factors Influencing the Occurrence of Unhealthy Tapirs in the Southern Yucatan Peninsula.

Information about the effects of environmental degradation on the health of terrestrial forest wildlife is limited, especially for rare species. In this study, we analyse the influence of ecological factors such as landscape characteristics and seasonality on the health status of Baird's tapirs in Calakmul, Mexico. We collected georeferenced photographic records of healthy (n = 32) and unhealthy (n = 22) tapirs from 2008 to 2019 and characterized landscape composition around each record at three spatial scales (circular buffers of 1, 2 and 3-km radii according to Baird's tapir home ranges). Our logistic model building process consisted in selecting the best spatial scale for each landscape cover class, before including them along with distance to human settlements and seasonality in a full model. The model that best explained the occurrence of unhealthy tapirs included the percentage of agriculture within a 1-km radius. This study hints at the negative effect that land-use change to agriculture occurring in Calakmul might have on tapir health, with 95.45% of unhealthy tapirs recorded in such landscapes. Further studies should investigate the proximate determinants of tapir health in anthropogenic landscapes, which might be linked to stress or to contact with domestic animals.

Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å.

Gap junctions establish direct pathways for cells to transfer metabolic and electrical messages. The local lipid environment is known to affect the structure, stability and intercellular channel activity of gap junctions; however, the molecular basis for these effects remains unknown. Here, we incorporate native connexin-46/50 (Cx46/50) intercellular channels into a dual lipid nanodisc system, mimicking a native cell-to-cell junction. Structural characterization by CryoEM reveals a lipid-induced stabilization to the channel, resulting in a 3D reconstruction at 1.9 Å resolution. Together with all-atom molecular dynamics simulations, it is shown that Cx46/50 in turn imparts long-range stabilization to the dynamic local lipid environment that is specific to the extracellular lipid leaflet. In addition, ~400 water molecules are resolved in the CryoEM map, localized throughout the intercellular permeation pathway and contributing to the channel architecture. These results illustrate how the aqueous-lipid environment is integrated with the architectural stability, structure and function of gap junction communication channels.

Gas-Phase Optical Detection of 3-Ethynylcyclopentenyl: A Resonance-Stabilized C7H7 Radical with an Embedded 1-Vinylpropargyl Chromophore.

The 3-ethynylcyclopentenyl radical (3ecpr) has been identified as the carrier of an electronic spectrum with origin at 21792 cm-1 using resonant ionization and laser-induced fluorescence spectroscopies. The radical was first detected in a toluene discharge and is most efficiently produced from 1,6-heptadiyne. Overwhelming spectroscopic and chemical evidence support our diagnosis: (1) the observed (6.93 eV) and calculated (CCSD(T)/pVQZ) adiabatic ionization energies are the same; (2) the origin band rotational contour can be well simulated with calculated rotational constants; (3) convincing vibrational assignments can be made using computed frequencies; and (4) the same spectrum was observed in a discharge of 1-ethynylcyclopentanol, which contains the 3ecpr carbon framework. The π-chromophore is essentially that of trans-1-vinylpropargyl, a highly resonance-stabilized C5H5 radical that persists in conditions relevant to both combustion and circumstellar atmospheres. We suggest that 3ecpr may be a similarly important radical warranting inclusion in models of C7H7 chemistry. It is the second C7H7 isomer with a five-membered ring yet to be detected, the other being vinylcyclopentadienyl, a species crucially involved in a recently proposed mechanism of soot formation (Science, 2018, 361, 6406, 997-1000). We argue that 3ecpr should be a significant product of H addition to ethynylcyclopentadiene (C7H6), a known product of benzyl decomposition. Further, it is plausible that 3ecpr is the unidentified C7H7 product of sequential addition of acetylene to propargyl (J. Phys. Chem. Lett., 2015, 6, 20, 4153-4158) in which 1-vinylpropargyl is an intermediate. As such, the nC2H2 + C3H3 cascade could represent a facile synthesis of a substituted five-membered ring in flames and stellar outflows.

Characterization of the serotonin 2A receptor selective PET tracer (R)-[18F]MH.MZ in the human brain.

PURPOSE: The serotonin receptor subtype 2A antagonist (5-HT2AR) (R)-[18F]MH.MZ has in preclinical studies been identified as a promising PET imaging agent for quantification of cerebral 5-HT2ARs. It displays a very similar selectivity profile as [11C]MDL 100907, one of the most selective compounds identified thus far for the 5-HT2AR. As [11C]MDL 100907, (R)-[18F]MH.MZ also displays slow brain kinetics in various animal models; however, the half-life of fluorine-18 allows for long scan times and consequently, a more precise determination of 5-HT2AR binding could still be feasible. In this study, we aimed to evaluate the potential of (R)-[18F]MH.MZ PET to image and quantify the 5-HT2AR in the human brain in vivo. METHODS: Nine healthy volunteers underwent (R)-[18F]MH.MZ PET at baseline and four out of these also received a second PET scan, after ketanserin pretreatment. Regional time-activity curves of 17 brain regions were analyzed before and after pretreatment. We also investigated radiometabolism, time-dependent stability of outcomes measures, specificity of (R)-[18F]MH.MZ 5-HT2AR binding, and performance of different kinetic modeling approaches. RESULTS: Highest uptake was determined in 5-HT2AR rich regions with a BPND of approximately 1.5 in cortex regions. No radiometabolism was observed. 1TCM and 2TCM resulted in similar outcome measure, whereas reference tissue models resulted in a small, but predictable bias. (R)-[18F]MH.MZ binding conformed to the known distribution of 5-HT2AR and could be blocked by pretreatment with ketanserin. Moreover, outcomes measures were stable after 100-110 min. CONCLUSION: (R)-[18F]MH.MZ is a suitable PET tracer to image and quantify the 5-HT2AR system in humans. In comparison with [11C]MDL 100907, faster and more precise outcome measure could be obtained using (R)-[18F]MH.MZ. We believe that (R)-[18F]MH.MZ has the potential to become the antagonist radiotracer of choice to investigate the human 5-HT2AR system.

Pharmacokinetic evaluation of [18F]PR04.MZ for PET/CT imaging and quantification of dopamine transporters in the human brain.

PURPOSE: Dopamine transporters (DAT) modulate pre-synaptic dopamine and physiological functions such as movement and reward. DAT also mirrors disease state in neurological disorders, rendering it an essential diagnostic target. [18F]PR04.MZ is a new PET imaging agent for DAT with an improved affinity and selectivity profile, for which we here describe the complete pharmacokinetic evaluation in healthy controls. METHODS: Thirty-two healthy subjects underwent T1-weighted MRI and dynamic PET scans for 180 min with arterial blood sampling (n = 5) or 90 min without blood sampling (n = 25) after injection of 197.6 ± 12.2 MBq [18F]PR04.MZ. Blood and plasma metabolite analysis were performed. MRI-based normalization of brain images, delineation of VOIs, and kinetic modeling was conducted to determine distribution volumes (Vt) and binding potentials (BPnd). The impact of scan duration was evaluated and repeated PET scans were performed to assess test-retest variability (n = 5). A static imaging protocol has been validated for clinical applications. RESULTS: [18F]PR04.MZ showed rapid metabolization in circulation, very high uptake in striatum and midbrain, and very low non-specific binding. The two-tissue compartment model 2TCM provided best fits for measured time-activity-curves and calculated Vts in putamen, caudate, substantia nigra pars compacta (SNpc), and cerebellar cortex were 11.83, 9.73, 2.12, and 0.57, respectively. All non-invasive models correlated well with BPnd values derived from 2TCM but underestimated DAT availability by about 28-33%. Of those, simplified reference tissue model (SRTM) provided the best fits, lowest Akaike Information Criteria values, and BPnd values of 14.82, 11.95, and 2.63 in putamen, caudate, and SNpc, respectively. BPnd estimates for striatal regions and SNpc were stable between 90 and 130 min post-injection. Test-retest results were excellent, showing low variability in all and excellent reliability in most relevant regions. Static imaging from 60 to 90-min post-injection is a viable alternative for quantification. CONCLUSIONS: [18F]PR04.MZ is a PET tracer with very high affinity, selectivity, and specific uptake in striatum and midbrain. 2TCM and SRTM provide good fits, high and stable Vts or BPnds, and good test-retest reliability for precise quantification of DAT in human subjects.

Waterhole detection using a vegetation index in desert bighorn sheep (Ovis canadensis cremnobates) habitat.

In arid ecosystems, desert bighorn sheep are dependent on natural waterholes, particularly in summer when forage is scarce and environmental temperatures are high. To detect waterholes in Sierra Santa Isabel, which is the largest area of desert bighorn sheep habitat in the state of Baja California, Mexico, we used the normalized difference vegetation index (NDVI) and normalized difference water index (NDWI) from Sentinel-2 satellite images. Waterhole detection was based on the premise that sites with greater water availability, where NDVI was higher, can be identified by their density of vegetation greenness. For the detected waterholes, we estimated the escape terrain (presence of cliffs or steep, rocky slopes) around each by the vector ruggedness measure to determine their potential use by desert bighorn sheep based on the animals' presence as documented by camera traps. We detected 14 waterholes with the NDVI of which 11 were known by land owners and 3 were unrecorded. Desert bighorn were not detected in waterholes with high values of escape terrain, i.e., flat areas. Waterhole detection by NDVI is a simple method, and with the assistance and knowledge of the inhabitants of the Sierra, it was possible to confirm the presence each waterhole in the field.

Hyper-phosphorylation of Rb S249 together with CDK5R2/p39 overexpression are associated with impaired cell adhesion and epithelial-to-mesenchymal transition: Implications as a potential lung cancer grading and staging biomarker.

Prediction of lung cancer metastasis relies on post-resection assessment of tumor histology, which is a severe limitation since only a minority of lung cancer patients are diagnosed with resectable disease. Therefore, characterization of metastasis-predicting biomarkers in pre-resection small biopsy specimens is urgently needed. Here we report a biomarker consisting of the phosphorylation of the retinoblastoma protein (Rb) on serine 249 combined with elevated p39 expression. This biomarker correlates with epithelial-to-mesenchymal transition traits in non-small cell lung carcinoma (NSCLC) cells. Immunohistochemistry staining of NSCLC tumor microarrays showed that strong phospho-Rb S249 staining positively correlated with tumor grade specifically in the squamous cell carcinoma (SCC) subtype. Strong immunoreactivity for p39 positively correlated with tumor stage, lymph node invasion, and distant metastases, also in SCC. Linear regression analyses showed that the combined scoring for phospho-Rb S249, p39 and E-cadherin in SCC is even more accurate at predicting tumor staging, relative to each score individually. We propose that combined immunohistochemistry staining of NSCLC samples for Rb phosphorylation on S249, p39, and E-cadherin protein expression could aid in the assessment of tumor staging and metastatic potential when tested in small primary tumor biopsies. The intense staining for phospho-Rb S249 that we observed in high grade SCC could also aid in the precise sub-classification of poorly differentiated SCCs.

Predicting Pinus monophylla forest cover in the Baja California Desert by remote sensing.

The Californian single-leaf pinyon (Pinus monophylla var. californiarum), a subspecies of the single-leaf pinyon (the world's only one-needled pine), inhabits semi-arid zones of the Mojave Desert (southern Nevada and southeastern California, US) and also of northern Baja California (Mexico). This tree is distributed as a relict subspecies, at elevations of between 1,010 and 1,631 m in the geographically isolated arid Sierra La Asamblea, an area characterized by mean annual precipitation levels of between 184 and 288 mm. The aim of this research was (i) to estimate the distribution of P. monophylla var. californiarum in Sierra La Asamblea by using Sentinel-2 images, and (ii) to test and describe the relationship between the distribution of P. monophylla and five topographic and 18 climate variables. We hypothesized that (i) Sentinel-2 images can be used to predict the P. monophylla distribution in the study site due to the finer resolution (×3) and greater number of bands (×2) relative to Landsat-8 data, which is publically available free of charge and has been demonstrated to be useful for estimating forest cover, and (ii) the topographical variables aspect, ruggedness and slope are particularly important because they represent important microhabitat factors that can determine the sites where conifers can become established and persist. An atmospherically corrected a 12-bit Sentinel-2A MSI image with 10 spectral bands in the visible, near infrared, and short-wave infrared light region was used in combination with the normalized differential vegetation index (NDVI). Supervised classification of this image was carried out using a backpropagation-type artificial neural network algorithm. Stepwise multiple linear binominal logistical regression and Random Forest classification including cross validation were used to model the associations between presence/absence of P. monophylla and the five topographical and 18 climate variables. Using supervised classification of Sentinel-2 satellite images, we estimated that P. monophylla covers 6,653 ± 319 ha in the isolated Sierra La Asamblea. The NDVI was one of the variables that contributed most to the prediction and clearly separated the forest cover (NDVI > 0.35) from the other vegetation cover (NDVI < 0.20). Ruggedness was the most influential environmental predictor variable, indicating that the probability of occurrence of P. monophylla was greater than 50% when the degree of ruggedness terrain ruggedness index was greater than 17.5 m. The probability of occurrence of the species decreased when the mean temperature in the warmest month increased from 23.5 to 25.2 °C. Ruggedness is known to create microclimates and provides shade that minimizes evapotranspiration from pines in desert environments. Identification of the P. monophylla stands in Sierra La Asamblea as the most southern populations represents an opportunity for research on climatic tolerance and community responses to climate variability and change.

Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis.

The retinoblastoma tumor suppressor protein (pRb) is a preeminent tumor suppressor that acts as a cell cycle repressor, specifically as an inhibitor of the G1-S transition of the cell cycle . pRb is a phosphoprotein whose function is repressed by extensive phosphorylation in several key residues, and therefore, pRb's phosphorylation status has become a surrogate for pRb activity. In particular, hyperphosphorylation of pRb has been associated with pathological states such as cancer, and therefore, assessing pRb's phosphorylation status is increasingly gaining diagnostic and prognostic value, may be used to inform therapeutic decisions, and is also an important tool for the cancer biologists seeking an understanding of the molecular etiology of cancer. In this chapter, we discuss an immunoblot protocol to detect pRb phosphorylation in two residues, serine 612 and threonine 821, in protein extracts from cancer cells.

Skin parasitism by Paratrichosoma recurvum in wild American crocodiles and its relation to environmental and biological factors.

Paratrichosma spp. are capillarid worms that parasitize the abdominal skin of crocodiles. They are likely not a threat to crocodiles' health, but they affect the skins' commercial value. No successful treatment exists against this parasite, and present knowledge of its life cycle is limited. Herein we report new information on Paratrichosoma recurvum occurrence in wild American crocodiles Crocodylus acutus from Mexican Caribbean islands and its relation to environmental (water salinity, temperature, climatic events) and biological (body condition) factors. The percentage of parasitized crocodiles (30.3%) is among the highest recorded in wild crocodilian populations. Small (<40.8 cm total length [TL]) and large (>270 cm TL) crocodiles are less parasitized, probably due to the characteristics of their skin or of the parasite life cycle. Two individuals appeared to have eliminated worms naturally between their capture and recapture. The thorax-abdomen is the most parasitized area of the body of crocodiles. The risk of infection is not associated with the sex of the crocodile, but there was a difference in the proportion of parasitized crocodiles between sites, which could be related to different environmental conditions. The body condition of a crocodile does not seem to be affected by the parasite. Climatic events and water temperature show no effect on the parasitism of crocodiles, but salinity could have an effect. The infection of crocodiles by P. recurvum could depend more on an individual's behavior than on environmental conditions.