Observation of α-Synuclein Preformed Fibrils Interacting with SH-SY5Y Neuroblastoma Cell Membranes Using Scanning Ion Conductance Microscopy.

Parkinson's disease (PD) is the second-most prevalent neurodegenerative disorder in the U.S. α-Synuclein (α-Syn) preformed fibrils (PFFs) have been shown to propagate PD pathology in neuronal populations. However, little work has directly characterized the morphological changes on membranes associated with α-Syn PFFs at a cellular level. Scanning ion conductance microscopy (SICM) is a noninvasive in situ cell imaging technique and therefore uniquely advantageous to investigate PFF-induced membrane changes in neuroblastoma cells. The present work used SICM to monitor cytoplasmic membrane changes of SH-SY5Y neuroblastoma cells after incubation with varying concentrations of α-Syn PFFs. Cell membrane roughness significantly increased as the concentration of α-Syn PFFs increased. Noticeable protrusions that assumed a more crystalline appearance at higher α-Syn PFF concentrations were also observed. Cell viability was only slightly reduced, though statistically significantly, to about 80% but independent of the dose. These observations indicate that within the 48 h treatment period, PFFs continue to accumulate on the cell membranes, leading to membrane roughness increase without causing prominent cell death. Since PFFs did not induce major cell death, these data suggest that early interventions targeting fibrils before further aggregation may prevent the progression of neuron loss in Parkinson's disease.

Tourists perceptions of Caribbean islands facing environmental threats before the COVID-19 health crisis: Holbox Island and Archipelago of Bocas del Toro.

Knowledge gaps exist in the socio-ecological systems of small touristic islands in Latin America. Understanding tourists' perceptions of their environmental knowledge can help plan actions to prevent natural capital loss necessary for local economies. Tourists' perceptions of a touristic hotspot, Holbox Island, were documented. Surveys demonstrated that tourists are aware of their environmental impacts and are interested in minimizing these. Results were compared with results on Bocas del Toro, Panama. Tourists' perceptions had similarities among sites driven by similarities in tourists' populations with a common geographic origin. Tourists lack site-specific knowledge to steer them towards environmentally conscious decisions in both regions. Findings suggest the need to promote local actions to gain tourists' understanding about their destination and support education programs on island conservation. Addressing these needs can help build resilience to overcome the adverse socio-environmental effects of tourism, environmental disasters, and health crises as COVID-19 on small islands.

Dirección de la cura y lengua de señas. El lugar de la falta. Un caso de angustia en pandemia / Direction of the cure and sign language. The place of lack. A case of anguish in the pandemic

Diana es hipoacúsica. El encierro, por el covid-19, le hizo sentir: necesito hacer algo para mí y no sé qué me gusta. Algo de su historia tiene que ver con esto, años de trabas e impedimentos, pero no a causa de su discapacidad, sino de su vida, su historia, su madre. Uno de los mecanismos esenciales del análisis es la noción de la falta de objeto. ¿Se trata de la falta de audición? Diana no sufre, no se queja de su sordera. Le pedimos al paciente hacerse cargo de sus dichos, entonces a una persona sorda, con deseo de análisis: ¿usar LSA le impediría hacerse cargo? La dirección de la cura apunta a pasar de los enunciados a la enunciación. La lengua de señas es un modo de enunciar. No es con el modo en que enuncia sino con sus enunciados que vamos a trabajar

Diana has hearing loss. Covid lockdown made her feel that she needed to do something for her, though she did not know what she liked. Something from her past history has something to do with that, years of obstacles and impediments, not because of her disability, but because of her life, her history, her mother. One of the essential mechanisms in the analysis is the notion of the lack of object. Is this about the hearing loss? Diana does not suffer; she does not complain about that. We ask the patient to take responsibility for what they say. Therefore, would the use of sign language become an obstacle to a person with hearing loss and willing to be analysed? The direction of the cure aims at passing from the enunciated to the enunciation. Sign language is a way to enunciate. We will work with her enunciations, not with the way she enunciates

Síndrome de Gorlin - Goltz en paciente pediátrico: Reporte de Caso

Introducción El síndrome de Gorlin-Goltz es una entidad infrecuente hereditario autosómica dominante por el gen PTCH ubicado en el cromosoma 9q22.3 de expresión variable. El padecimiento es caracterizado por desarrollar carcinomas basocelulares, queratoquistes odontogénicos, meduloblastomas y fibromas ováricos. Objetivo Conocer las características clínicas y patológicas para un adecuado diagnóstico y tratamiento. Presentación del caso Paciente femenino de 13 años de edad acude a consulta al posgrado de Odontología Infantil de la Facultad de odontología Unidad Torreón de la Universidad Autónoma de Coahuila. Clínicamente presenta un aumento de volumen hemifacial izquierdo, indoloro, del color de la piel circundante, normotérmico, blando; así mismo, puente nasal ancho, exoftalmos y protuberancia frontal. A la exploración intraoral, se observa aumento de volumen en fondo de vestíbulo maxilar izquierdo extendiéndose de canino a premolares; en mandíbula aumento de volumen en fondo de vestíbulo derecho que abarca desde el central izquierdo hasta el molar derecho. En la ortopantomografía se observan 3 lesiones radiolúcidas en maxilar y dos en mandíbula provocando desplazamiento dental. Conclusión El diagnóstico temprano de este síndrome evitará confundir los tumores odontogénicos queratoquísticos con procesos infecciosos y así logar obtener resultado sin morbilidad elevada.

Serious postpartum infection due to MRSA in an asymptomatic carrier: case report and review.

Infection with methicillin-resistant Staphylococcus aureus (MRSA) has become a worldwide problem and is no longer acquired only in a hospital setting. Community-associated MRSA is an emerging pathogen of increasing interest to both obstetricians and neonatologists, reported in all three trimesters of pregnancy and postpartum, and in neonatal intensive care units, leading to severe outcomes, including neonatal death. This case report describes a serious and potentially life-threatening infection (including wound abscess, septicemia, septic thrombophlebitis, and septic pulmonary emboli) that developed in an otherwise healthy postpartum woman who had screened positive for MRSA in nares, vagina, and rectum at the time of her prior admission in labor as part of a research study. We conclude that asymptomatic nasal, vaginal, and rectal colonization with MRSA occurs in pregnancy and may be a risk factor for serious systemic infection after delivery.