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1.
Plants (Basel) ; 13(6)2024 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-38592828

RESUMO

Common bean (Phaseolus vulgaris L.) is the primary grain legume cultivated worldwide for direct human consumption due to the high nutritional value of its seeds and pods. The high protein content of common beans highlights it as the most promising source of plant-based protein for the food industry. Additionally, landraces of common bean have great variability in nutritional traits, which is necessary to increase the nutritional quality of elite varieties. Therefore, the main objective of this study was to nutritionally characterize 23 Chilean landraces and 5 commercial varieties of common bean to identify genotypes with high nutritional value that are promising for the food industry and for genetic improvement programs. The landrace Phv23 ('Palo') was the most outstanding with high concentrations of minerals such as P (7.53 g/kg), K (19.8 g/kg), Mg (2.43 g/kg), Zn (52.67 mg/kg), and Cu (13.67 mg/kg); essential amino acids (364.8 mg/g protein); and total proteins (30.35 g/100 g seed). Additionally, the landraces Phv9 ('Cimarrón'), Phv17 ('Juanita'), Phv3 ('Araucano'), Phv8 ('Cabrita/Señorita'), and Phv4 ('Arroz') had a high protein content. The landrace Phv24 ('Peumo') stood out for its phenolic compounds (TPC = 218.1 mg GA/100 g seed) and antioxidant activity (ORAC = 22,167.9 µmol eq trolox/100 g extract), but it has moderate to low mineral and protein concentrations. In general, the concentration of nutritional compounds in some Chilean landraces was significantly different from the commercial varieties, highlighting their high nutritional value and their potential use for the food industry and for genetic improvement purposes.

2.
Can J Infect Dis Med Microbiol ; 2024: 8871439, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38384428

RESUMO

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19). It is estimated that more than half of new infections are transmitted by asymptomatic people; therefore, the isolation of symptomatic people is not enough to control the spread of the disease. Methods: A total of 171 unvaccinated young adults (18-35 years) from Sonora, Mexico, who underwent a structured survey to identify prior COVID-19 infections, were included in this study. A qualitative determination of anti-SARS-CoV-2 antibodies in serum was performed by lateral flow immunoassay (Certum IgG/IgM Rapid Test™ cassette kit) and neutralizing antibodies were also determined (GenScript cPass assay). Results: A total of 36 people reported a history of COVID-19 infection, and 135 reported no history of COVID-19. In contrast, 49.6% (67/135) of individuals who had not reported a previous SARS-CoV-2 infection were seropositive to the rapid anti-SARS-CoV-2 antibody test, and 48.1% (65/135) of them had neutralizing antibodies. Conclusions: These results suggest that in young adults, SARS-CoV-2 infections could be asymptomatic in a high percentage of individuals, which could contribute in part to the slow control of the current pandemic due to the large number of asymptomatic cases that are contagious and that could be a silent spread of the virus.

3.
J Agric Food Chem ; 72(9): 4897-4905, 2024 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-38394028

RESUMO

The potential use of texturized pea protein in meat analogues was investigated by comparing the effects of fermentation on pea and myofibrillar pork proteins in a model system including additives, microbial starters, and proteases. Model fermentation was controlled for 15 days by a pH decrease and microbial count and free amino acid increase. Besides, volatile production and sensory properties were evaluated at the end of fermentation. Protein type affected free amino acid generation and volatile profile. Models supplemented with proteases showed an increase in amino-acid-derived compounds (branched aldehydes and alcohols) and fruity odor notes. During fermentation, protease addition significantly reduced the production of linear aldehydes (pentanal, hexanal, and octanal) in vegetal models, while pyrazine compounds were not affected. This changes in the volatile profile reduced the legume beany odor but increased the perception of toasted cereal-like notes generated by the texturization process.


Assuntos
Proteínas de Ervilha , Compostos Orgânicos Voláteis , Peptídeo Hidrolases , Odorantes , Fermentação , Substitutos da Carne , Aldeídos , Endopeptidases , Aminoácidos
4.
medRxiv ; 2024 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-38352327

RESUMO

Background: Understanding the relative contributions of SARS-CoV-2 infection-induced and vaccine- induced seroprevalence is key to measuring overall population-level seroprevalence and help guide policy decisions. Methods: Using a series of six population-based cross-sectional surveys conducted among persons aged ≥7 years in a large health system with over 4.5 million members between May 2021 and April 2022, we combined data from the electronic health record (EHR), an electronic survey and SARS-CoV-2 spike antibody binding assay, to assess the relative contributions of infection and vaccination to population- level SARS-CoV-2 seroprevalence. EHR and survey data were incorporated to determine spike antibody positivity due to SARS-CoV-2 infection and COVID-19 vaccination. We used sampling and non-response weighting to create population-level estimates. Results: We enrolled 4,319 persons over six recruitment waves. SARS-CoV-2 spike antibody seroprevalence increased from 83.3% (CI 77.0-88.9) in May 2021 to 93.5% (CI 89.5-97.5) in April 2022. By April 2022, 68.5% (CI 61.9-74.3) of the population was seropositive from COVID-19 vaccination only, 13.9% (10.7-17.9) from COVID-19 vaccination and prior diagnosed SARS-CoV-2 infection, 8.2% (CI 4.5- 14.5) from prior diagnosed SARS-CoV-2 infection only and 2.9% (CI 1.1-7.6) from prior undiagnosed SARS-CoV-2 infection only. We found high agreement (≥97%) between EHR and survey data for ascertaining COVID-19 vaccination and SARS-CoV-2 infection status. Conclusions: By April 2022, 93.5% of persons had detectable SARS-CoV-2 spike antibody, predominantly from COVID-19 vaccination. In this highly vaccinated population and over 18 months into the pandemic, SARS-CoV-2 infection without COVID-19 vaccination was a small contributor to overall population-level seroprevalence. Article summary: By April 2022, >93% of people had antibodies to SARS-CoV-2 with COVID-19 vaccination as the main driver of overall population-level seroprevalence in our healthcare system. SARS-CoV-2 infection without vaccination made a small contribution to population-level seroprevalence in our healthcare system.

5.
Mol Phylogenet Evol ; 190: 107945, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37863452

RESUMO

The deep-sea has experienced dramatic changes in physical and chemical variables in the geological past. However, little is known about how deep-sea species richness responded to such changes over time and space. Here, we studied the diversification dynamics of one of the most diverse octocorallian families inhabiting deep sea benthonic environments worldwide and sustaining highly diverse ecosystems, Primnoidae. A newly dated species-level phylogeny was constructed to infer their ancestral geographic locations and dispersal rates initially. Then, we tested whether their global and regional (the Southern Ocean) diversification dynamics were mediated by dispersal rate and abiotic factors as changes in ocean geochemistry. Finally, we tested whether primnoids showed changes in speciation and extinction at discrete time points. Our results suggested primnoids likely originated in the southwestern Pacific Ocean during the Lower Cretaceous âˆ¼112 Ma, with further dispersal after the physical separation of continental landmasses along the late Mesozoic and Cenozoic. Only the speciation rate of the Southern Ocean primnoids showed a significant correlation to ocean chemistry. Moreover, the Paleocene-Eocene thermal maximum marked a significant increase in the diversification of primnoids at global and regional scales. Our results provide new perspectives on the macroevolutionary and biogeographic patterns of an ecologically important benthic organism typically found in deep-sea environments.


Assuntos
Antozoários , Ecossistema , Humanos , Animais , Filogenia , Oceano Pacífico
7.
IDCases ; 34: e01892, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37711881

RESUMO

Hyper-reactive malarial splenomegaly (HMS), or tropical splenomegaly syndrome, is a severe complication of chronic and recurrent infections caused by Plasmodium spp. This condition typically results in splenomegaly greater than or equal to 10 cm and a constellation of laboratory findings, including the absence of identifiable parasites in peripheral blood smears. However, patients with HMS demonstrate serological or molecular evidence of infection. Despite being a familiar entity in malaria holoendemic countries in Africa, and regions of Papua New Guinea, the pathophysiology, natural history, and treatment of the syndrome remains to be fully elucidated. Herein, we describe a highly suggestive case of HMS in a Senegalese patient migrating northbound to reach the U.S.-Mexico border and for whom we provided medical care during his crossing of the Darien Gap in Panama. We also reviewed the literature on diagnosing and treating HMS in-depth.

8.
Cureus ; 15(5): e39703, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-37398802

RESUMO

Necrotizing infections of deep neck spaces are a group of life-threatening infectious diseases acquired through trauma or as a descending infection from an odontogenic source. The isolation of pathogens is unusual because of the anaerobic nature of the infection; however, one way to achieve this is through the use of automated microbiological methods like matrix-assisted laser desorption/ionization and time-of-flight (MALDI-TOF) following standard microbiology protocols for analyzing samples from potential anaerobic infections. We present a case of a patient without risk factors for descending necrotizing mediastinitis with isolation of Streptococcus anginosus and Prevotella​​​​​​​ buccae managed at the intensive care unit with a multidisciplinary team. We present our approach and how we successfully treat this complicated infection.

9.
Foods ; 12(12)2023 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-37372640

RESUMO

This study assessed the effect of replacing pork lard with coconut oil and Debaryomyces hansenii inoculation on the biotransformation of amino acids into volatile compounds in a meat model system. Yeast counts, solid-phase microextraction, and gas chromatography/mass spectrometry were used to assess yeast growth and volatile production, respectively. Yeast growth was confirmed until 28 d, although the volatile profile changed until 39 d. Forty-three volatiles were quantified, and their odor activity values (OAVs) were calculated. The presence of fat and yeasts contributed to differences in volatiles. In pork lard models, a delayed formation of lipid-derived aldehyde compounds was observed, whereas in coconut oil models, the generation of acid compounds and their respective esters was enhanced. Yeast activity affected amino acid degradation, which produced an increase in branched-chain aldehydes and alcohols. The aroma profile in the coconut models was influenced by hexanal, acid compounds, and their respective esters, whereas in pork lard models, aroma was affected by methional (musty, potato) and 3-methylbutanal (green, cocoa). The yeast inoculation contributed to the generation of 3-methylbutanoic acid (cheesy) and phenylethyl alcohol (floral). The type of fat and yeast inoculation produced a differential effect on the aroma.

10.
Cambios rev. méd ; 22(1): 894, 30 Junio 2023. ilus, tabs
Artigo em Espanhol | LILACS | ID: biblio-1451329

RESUMO

La fisura anal es una de las enfermedades más antiguamente descritas, la misma que, ha tenido hasta el momento múltiples tratamientos tanto médicos como quirúrgicos, existiendo controversias en su algoritmo terapéutico. Constituye una de las patologías cuyo diagnóstico y tratamiento corresponde a la Especialidad de Coloproctología, afecta a ambos sexos y a cualquier edad y puede ser aguda o crónica. Proponemos el presente Protocolo para un adecuado manejo de la patología, de manera que sirva de guía en la toma correcta de decisiones basadas en la evidencia y el consenso de quienes integramos la Unidad Técnica de Coloproctología del Hospital de Especialidades Carlos Andrade Marín.


Anal fissure is one of the oldest described diseases, which has so far had multiple medical and surgical treatments, with controversies in its therapeutic algorithm. It is one of the pathologies whose diagnosis and treatment corresponds to the Coloproctology Specialty, it affects both sexes and any age and can be acute or chronic. We propose the present Protocol for an adequate management of the pathology, so that it serves as a guide in the correct decision making based on evidence and consensus of those who integrate the Technical Unit of Coloproctology of the Hospital de Especialidades Carlos Andrade Marín.


Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Canal Anal , Doenças do Ânus , Prurido Anal , Cirurgia Colorretal , Fissura Anal/cirurgia , Qualidade de Vida , Proctoscopia , Dieta , Equador , Esfincterotomia Lateral Interna , Hemorragia , Analgesia
11.
J Sci Food Agric ; 103(6): 2806-2814, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36647301

RESUMO

BACKGROUND: Hybrid batters constitute the base for the processing of cooked and dry meat analogues. The use of texturized plant proteins in their formulation is a key strategy to reduce the consumption of animal proteins, although off-flavors present in these plant proteins often cause sensory rejection. The aim was to study the effect of a deodorization process of pea protein, for their use in hybrid meat batters at different percentages of substitution. RESULTS: Hybrid patties with higher percentages of pea protein showed higher values of yellowness, pH, and water activity, whereas hardness was reduced. Soaking treatment with ethanol for deodorization of the texturized pea protein increased humidity and pH in all patties but reduced the textural properties (hardness, springiness, cohesiveness, and chewiness). The addition of oat flour improved the patty texture at higher percentages of pea protein, but in soaked deodorized patty formulations it affected volatile retention. Volatile compounds related to off-flavors (aldehydes, alcohols, acid compounds, ketones, and pyrazines) appeared significantly increased as the pea percentage increased, although these were efficiently removed by the soaking deodorizing process applied. CONCLUSION: Soaking of the texturized pea protein with ethanol is an effective strategy to reduce off-flavors in hybrid meat patties. However, it produces changes in the textural characteristics by the solubilization of the proteins of the texturized pea, reducing the availability to form a network and affecting volatile retention. The formulation of hybrid batter should be controlled in all sensory aspects for the processing of cooked and dry cured meat product analogues. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Assuntos
Produtos da Carne , Proteínas de Ervilha , Animais , Carne/análise , Culinária , Produtos da Carne/análise , Proteínas , Solventes
12.
Cureus ; 15(12): e51247, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38283480

RESUMO

Caterpillar venom has the potential to cause acute pain and systemic symptoms in individuals seeking medical attention in the jungles of Panama. Although this is not an obligatory notifiable disease, the hazards associated with exposure to this animal are widely recognized within the local community. Here, we present a case of a patient who sought medical attention after being rescued from a river in a Panamanian jungle after feeling acute pain in an upper extremity associated with shortness of breath and how tropical medicine teleconsult allowed for quick identification of the cause and assisted in the management. About his case, we examine the phenomenon of caterpillar envenomation and suggest that further research is needed to assess the potential impact of climate change on this disease. Of particular concern is the likelihood of an increase in contact accidents. We recommend that scientists and public health officials work together to understand the mechanisms of this disease better and to develop effective strategies for prevention and treatment. Our analysis underscores the importance of ongoing monitoring and surveillance to ensure we are prepared for future outbreaks.

13.
Curr Issues Mol Biol ; 44(9): 4268-4281, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36135205

RESUMO

Citrullination is catalyzed by the peptidyl arginine deiminase 4 (PAD4) enzyme, encoded by the PADI4 gene. Increased PAD4 activity promotes the onset and progression of rheumatoid arthritis (RA). This study aimed to evaluate the association of PADI4 haplotypes with RA risk, mRNA expression, and the PAD4 activity in patients with RA from Mexico. Methodology: 100 RA patients and 100 control subjects (CS) were included. Genotyping was performed by PCR-RFLP method, PADI4 mRNA expression was quantified by real-time PCR, the contribution of PADI4 alleles (PADI4_89 G>A, PADI4_90 T>C, and PADI4_92 G>C) to mRNA expression by the ASTQ method, and PAD4 activity by HPLC. Also, the anti-CCP and anti-PADI4 antibodies were quantified by ELISA. Results: The three PADI4 polymorphisms were associated with RA susceptibility (OR = 1.72, p = 0.005; OR = 1.62; p = 0.014; OR = 1.69; p = 0.009; respectively). The 89G, 90T, and 92G alleles have a higher relative contribution to PADI4 mRNA expression from RA patients than 89A, 90C, and 92C alleles in RA patients. Moreover, the GTG/GTG haplotype was associated with RA susceptibility (OR = 2.86; p = 0.024). The GTG haplotype was associated with higher PADI4 mRNA expression (p = 0.04) and higher PAD4 enzymatic activity (p = 0.007) in RA patients. Conclusions: The evaluated polymorphisms contribute to PADI4 mRNA expression and the enzymatic activity of PAD4 in leukocytes. Therefore, the GTG haplotype is a genetic risk factor for RA in western Mexico, and is associated with increased PADI4 mRNA expression and higher PAD4 activity in these patients.

15.
Meat Sci ; 192: 108896, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35753267

RESUMO

The goal of this study was to confirm and acquire more information about the nitrogen and sulfur compounds existing in the volatile profile of dry fermented sausages from the addition of precursors (proline, ornithine and thiamine), and their role in sausage aroma. To this end, the precursors were added to the formulation of sausages, which were submitted to a fermentation and drying process. The sausage aroma was analyzed by olfactometry technique and Free Choice Profile sensory analysis. The results showed that the addition of precursors impacted the aroma, and reduced the level of oxidation in the final sausages while microbial differences were mainly observed in Orn-sausages. Among the aroma compounds detected only 2-methyl-3-(methylthio)furan verified the effect of thiamine supplementation and the impact on the cured and savoury odours detected in Thia-sausages by Free Choice profile sensory analysis, while no clear effect could be attributed to specific volatile compounds in the nitrogen supplemented sausages.


Assuntos
Produtos da Carne , Odorantes , Fermentação , Produtos da Carne/análise , Nitrogênio , Odorantes/análise , Enxofre , Tiamina
16.
Cureus ; 14(3): e23158, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35444888

RESUMO

Surgical site infections are a common complication in the management of patients with solid cancer tumors in cytotoxic chemotherapy. The antibiotic combination chosen depends on the neoadjuvant therapy used and the anatomical site and extent of the surgery. In this brief report, we describe a surgical site infection caused by an unusual microorganism and a succinct review of the pathogen's behavior.

17.
Podium (Pinar Río) ; 17(1)abr. 2022.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1448864

RESUMO

La recuperación es un estado funcional del deportista una vez que el estímulo físico se detiene, lo que es esencial para la bioadaptación del organismo. El entrenamiento funcional, al simular una actividad de la vida cotidiana de forma integral, puede ser utilizado para la recuperación muscular, es una alternativa para la recuperación poscompetencia en futbolistas de iniciación. En tal sentido, se plantea como propósito demostrar si el entrenamiento funcional permite la recuperación poscompetencia en futbolistas Sub-12. La investigación es transversal-correlacional, de orientación cualitativa, estudia a una población de 21 jugadores de fútbol Sub-12, a los cuales se les implementó una propuesta de intervención con ejercicios funcionales, se les midió el índice de fatiga en dos momentos de la preparación y se consultaron los criterios de 13 especialistas. Las siete fases del test Bangsbo (Pretest) disminuyeron su efectividad en la medida que se sucedieron las pruebas (Prueba 1: 7.35s; Prueba 7: 6.99s) y en el postest, la efectividad fue mejor (Min: 7.59s a Máx: 7.86s), se redujo el índice de fatiga en 1 % (de 1,55s a 0.78s). Por otra parte, los especialistas no consideraron que el entrenamiento funcional posee una influencia significativa en la recuperación poscompetencia (2.54 puntos Baja y Media). La aplicación del entrenamiento funcional evidenció mejoras en el índice de fatiga, resulta una alternativa efectiva para la recuperación poscompetencia en fútbol Sub-12, aunque los especialistas consultados desde el punto de vista teórico no han considerado la importancia que posee el entrenamiento funcional para optimizar los procesos de recuperación orgánica.


A recuperação é um estado funcional do atleta quando o estímulo físico pára, o que é essencial para a bi adaptação do organismo. O treino funcional, através da simulação de uma atividade da vida quotidiana de forma integral, pode ser utilizado para a recuperação muscular, é uma alternativa para a recuperação pós-competição em jogadores de futebol principiantes. Neste sentido, o objectivo deste estudo é demonstrar se o treino funcional permite a recuperação pós-competição em jogadores de futebol sub-12. A investigação é transversal-correlacional, de orientação qualitativa, estuda uma população de 21 jogadores de futebol sub-12, aos quais foi implementada uma proposta de intervenção com exercícios funcionais, o índice de fadiga foi medido em dois momentos da preparação e foram consultados os critérios de 13 especialistas. As sete fases do teste Bangsbo (Pré-teste) diminuíram em eficácia à medida que os testes prosseguiam (Teste 1: 7,35s; Teste 7: 6,99s) e no pós-teste, a eficácia foi melhor (Min: 7,59s para Max: 7,86s), o índice de fadiga foi reduzido em 1% (de 1,55s para 0,78s). Por outro lado, a formação funcional não foi considerada pelos especialistas como tendo uma influência significativa na recuperação pós-competição (2,54 pontos Baixo e Médio). A aplicação do treino funcional mostrou melhorias no índice de fadiga, é uma alternativa eficaz para a recuperação pós-competição no futebol sub-12, embora os especialistas consultados do ponto de vista teórico não tenham considerado a importância do treino funcional para optimizar os processos de recuperação orgânica.


Recovery is a functional state of the athlete once the physical stimulus stops, which is essential for the bioadaptation of the organism. Functional training, by simulating an activity of daily life in an integral way, can be used for muscle recovery, and is an alternative for post-competition recovery in beginner soccer players. In this sense, the purpose of this study is to demonstrate whether functional training allows post-competition recovery in U12 soccer players. The research is transversal-correlational, of qualitative orientation, it studies a population of 21 U-12 soccer players, to whom an intervention proposal with functional exercises was implemented, the fatigue index was measured in two moments of the preparation and the criteria of 13 specialists were consulted. The seven phases of the Bangsbo test (Pretest) decreased their effectiveness as the tests went on (Test 1: 7.35s; Test 7: 6.99s) and in the posttest, the effectiveness was better (Min: 7.59s to Max: 7.86s), the fatigue index was reduced by 1 % (from 1.55s to 0.78s). On the other hand, the specialists did not consider that functional training has a significant influence on post competition recovery (Low and Medium 2.54points). The application of functional training evidenced improvements in the fatigue index, it is an effective alternative for post competition recovery in U-12 soccer, although the specialists consulted from the theoretical point of view have not considered the importance of functional training to optimize the processes of organic recovery.

18.
Travel Med Infect Dis ; 47: 102317, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35342009

RESUMO

Rapid rise of population migration is a defining feature of the 21st century due to the impact of climate change, political instability, and socioeconomic downturn. Over the last decade, an increasing number of migrant peoples travel across the Americas to reach the United States seeking asylum or cross the border undocumented in search of economic opportunities. In this journey, migrant people experience violations of their human rights, hunger, illness, violence and have limited access to medical care. In the 'Divine Comedy', the Italian poet Dante Alighieri depicts his allegorical pilgrimage across Hell and Purgatory to reach Paradise. More than 700 years after its publication, Dante's poem speaks to the present time and the perilious journey of migrant peoples to reach safehavens. By exploring the depths and heights of the human condition, Dante's struggles resonate with the multiple barriers and the unfathomable experiences faced by migrant peoples in transit across South, Central, and North America to reach the United States. Ensuring the safety of migrant peoples across the Americas and elsewhere, and attending to their health needs during their migratory paths represent modern priorities to reduce social injustices and achieving health equity.


Assuntos
Migrantes , América , Países em Desenvolvimento , Humanos , Itália , Dinâmica Populacional , Estados Unidos
19.
Vaccines (Basel) ; 10(3)2022 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-35335032

RESUMO

Several studies have reported the benefits and safety of heterologous vaccination among different approved vaccines; however, there are no specific reports on the effects of vaccination with the Ad5-nCoV and other vaccines of the same or different technologies. In the present study, we evaluated the neutralizing antibodies percentage against SARS-CoV-2 in Mexican patients immunized with the Ad5-nCoV vaccine six months after its application. Moreover, the effect of the heterologous vaccination with the Ad5-nCoV vaccine and a booster dose of ChAdOx1-S-Nov-19, Ad26.COV2.S, BNT162b2, or mRNA-127 were determined. Our results suggest that a heterologous regimen of one dose with Ad5-nCoV vaccine followed by a booster dose of a different vaccine is safe and induces a stronger humoral immune response.

20.
J Appl Microbiol ; 133(1): 200-211, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35050543

RESUMO

INTRODUCTION: Debaryomyces hansenii is a yeast widely used in meat fermentations as starter for the purpose of improving the aromatic quality of the final product. However, it has not been the subject of an extensive study regarding phenotypic characteristics important for starter selection, such as the capacity to grow at abiotic stress conditions occurring during fermentation, the ability to generate desirable aromas and the absence of virulence traits in yeasts. AIMS: The aim of this study was to screen 60 strains of D. hansenii isolated from assorted foods for their potential application as starters in dry-cured fermented sausages manufacture. METHODS: The abiotic stress factors tested were low aw and pH and high concentration of salt, acetic acid and lactic acid. The phenotypic virulence traits explored were growth at 37°C, pseudohyphal and biofilm generation, invasiveness and enzymatic activities present in virulent yeasts. The generation of desirable meat aromas was tested in models containing aroma precursors applying an olfactory analysis. A quantitative profiling of stress tolerance was used to test the potential performance of selected strains in meat fermentations. RESULTS: The results demonstrated that most strains displayed no virulence trait or were only positive for biofilm production. Moreover, the strains showed large heterogeneity regarding their tolerance to abiotic stress factors, although most of them could grow at intermediate to high levels of the traits. The sensory analysis was the criteria determining the selection of starter strains. CONCLUSIONS: The evaluation of the phenotypic traits demonstrates that D. hansenii is a safe yeast, it is able to tolerate the stress in meat fermentation and it is able to generate desirable aromas. SIGNIFICANCE AND IMPACT OF THE STUDY: The results of this study confirm the adequacy of selected D. hansenii strains to be applied as starters in meat products.


Assuntos
Debaryomyces , Produtos da Carne , Debaryomyces/genética , Fermentação , Microbiologia de Alimentos , Odorantes/análise , Saccharomyces cerevisiae , Estresse Fisiológico , Fatores de Virulência/análise
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