RESUMO
INTRODUCTION: Brucellosis is a disease of high morbidity that affects several animal species, is transmitted to humans and, therefore, is a zoonosis. It is caused by bacteria of the genus Brucella. In this study we aim to determine seroprevalence, risk factors, and spatial distribution of caprine brucellosis in 14 municipalities in the central region of the state of Veracruz. MATERIALS AND METHODS: This cross-stratified multistage study was conducted between 2009 and 2012. It included 572 animals of 81 production units selected by consensus according to the value tables of ââCannon and Roe. The diagnosis was by Card Testing and Radial Immunodiffusion. The seroprevalence was determined with the VassarStats® risk factor program and odds. RESULTS: The overall seroprevalence was 0.52% (95% CI: 0.13-1.65) and production units 2.47% (95% CI: 0.43-9.46). They were identified as risk factor for infection, production units in feedlot system and Card Testing seroconversion to vaccine against brucellosis; and as a protective factor, vaccination. CONCLUSIONS: Seroprevalence and distribution of goat brucellosis is low, the intensive system is a risk, and according with the Health Ministry in order that human cases are scarce.
Assuntos
Brucelose/veterinária , Doenças das Cabras/epidemiologia , Doenças das Cabras/microbiologia , Animais , Brucelose/sangue , Brucelose/epidemiologia , Estudos Transversais , Feminino , Doenças das Cabras/sangue , Cabras , Masculino , México/epidemiologia , Estudos SoroepidemiológicosRESUMO
The protective dose of a live recombinant LaSota Newcastle disease virus (NDV)-avian influenza H5 vaccine (rNDV-LS/AI-H5) was determined in broiler chickens with high levels of maternal antibodies against NDV and avian influenza virus (AIV). At hatch the geometric mean titers (GMT) of the chickens' maternal antibodies were 2(5.1) and 2(10.3) for NDV and AIV, respectively. At the time of vaccination the GMT was 2(3.1) for NDV and 2(7.9) for AIV. The chickens were vaccinated with one drop (0.03 ml) in the eye at 10 days of age as is typical under field conditions. The test chickens received 10(4.8), 10(5.8), 10(6.8), or 10(7.8) mean chicken embryo infective doses (CEID50) of the rNDV-LS/AI-H5 vaccine. Control chickens were either nonvaccinated, or vaccinated with 10(5.8) or 10(6.8) CEID50 of a commercial live LaSota NDV vaccine. Birds were challenged with either the Mexican highly pathogenic avian influenza virus (HPAIV) strain A/Chicken/Queretaro/14588-19/95 (H5N2) or a Mexican velogenic viscerotropic (VV) NDV strain. One hundred percent of the chickens vaccinated with the rNDV-LS/AI-H5 vaccine were protected against HPAIV and VVNDV when a challenge dose of 10(6.8) EID50 or higher was administered by eye drop. Birds vaccinated with the LaSota NDV vaccine were protected against VVNDV, but not against HPAIV.
Assuntos
Galinhas , Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia , Vírus da Influenza A Subtipo H5N2/imunologia , Vacinas contra Influenza/imunologia , Influenza Aviária/prevenção & controle , Vírus da Doença de Newcastle/imunologia , Animais , Anticorpos Antivirais/sangue , Feminino , Imunidade Materno-Adquirida , Vírus da Influenza A/imunologia , Influenza Aviária/imunologia , Influenza Aviária/virologia , Masculino , Doença de Newcastle/sangue , Doença de Newcastle/imunologia , Doença de Newcastle/prevenção & controle , Vírus da Doença de Newcastle/classificação , Vacinas Sintéticas/imunologiaRESUMO
Specific-pathogen-free chickens immunized at 14 days of age with either an inactivated recombinant Newcastle disease virus-LaSota/avian influenza H5 (K-rNDV-LS/AI-H5) vaccine or a killed Newcastle disease/avian influenza whole-virus vaccine (K-ND/AI) were protected from disease when challenged with either A/chicken/Queretaro/14588-19/95 (H5N2), a high pathogenicity avian influenza virus (HPAIV) strain isolated in Mexico in 1995, or with a Mexican velogenic viscerotropic Newcastle disease virus (VVNDV) strain 21 days postvaccination. All nonvaccinated chickens challenged with HPAIV or VVNDV succumbed to disease, while those vaccinated with K-rNDV-LS/AI-H5 or K-ND/AI were protected from severe clinical signs and death. Both vaccines induced hemagglutination-inhibition (HI) antibody responses against NDV and AIV. Antibodies against AIV nucleoprotein were not detected by enzyme-linked immunosorbent assay (ELISA) in birds vaccinated with the inactivated rNDV-LS/AI-H5 vaccine. These chickens became positive for AIV antibodies by ELISA only after challenge with HPAIV. The data clearly indicate that the inactivated rNDV-LS/AI-H5 vaccine confers protection comparable to that of the conventional killed whole-virus vaccine against both NDV and AIV, while still allowing differentiation of infected from vaccinated animals by HI and ELISA tests.
Assuntos
Galinhas , Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia , Influenza Aviária/prevenção & controle , Doença de Newcastle/prevenção & controle , Vírus da Doença de Newcastle/imunologia , Vacinas Virais/imunologia , Animais , Ensaio de Imunoadsorção Enzimática , Feminino , Influenza Aviária/imunologia , Influenza Aviária/virologia , Masculino , Doença de Newcastle/imunologia , Doença de Newcastle/virologia , Organismos Livres de Patógenos Específicos , Vacinas de Produtos Inativados/imunologia , Vacinas Sintéticas/imunologia , Vacinas Virais/administração & dosagemRESUMO
Zoonoses are at the present time more important than ever due to their magnitude and impact. The international trade in animals, products and sub products, as well as the intense travel of people around the world, represent risks of dissemination of infectious diseases, and are the reason for a new age of emerging and reemerging zoonotic diseases. Under these conditions, public health and animal health authorities are obliged to work together in order to get more efficient control programs. In this paper the actual situation of some important emerging and reemerging zoonoses is analyzed, including: anthrax, rabies, tuberculosis, brucellosis, cysticercosis, echinococcosis, hanta virus, Hendra and Nipah virus. Particular attention is given to leptospirosis, due to the fact that it is considered by WHO and OIE as the widest spread zoonotic disease in the world. Zoonoses caused by ingestion of animal food products are discussed. They are responsible for the death of almost 2.2 million people. Bacteria of genus salmonella and campylobacter are considered. Some recommendations are given for the control and prevention of zoonoses, emphasizing the "One Health" concept.
Assuntos
Zoonoses/epidemiologia , Animais , Saúde GlobalRESUMO
Para evaluar el efecto del Factor de Transferencia (FT) en la respuesta leucocitaria en becerros clínicamente enfermos, se formaron tres grupos de diez animales cada uno. El grupo I (testigo) fue tratado con antibióticos, el II con FT más antibióticos y el III con la aplicación de tres dosis de FT (una cada tres días). Se tomaron muestras sanguíneas a todos los animales a los 0, 3, 5 y 14 días postratamiento, con el fin de realizar pruebas de Biometría Hemática y Rosetas "E" (para evaluar niveles de linfocitos "T"). Todos los animales se mantuvieron en observacióne durante 60 días (periodo de lactancia). Los resultados obtenidos fueron: El grupo I (testigo) tuvo un aumento de 3.87 porciento en la cuenta de roseras "E", el cual no fue significativo (P>0.05). Asimismo, los días de recuperación del cuadro clínico respiratorio (Neumonía) y digestivo (Síndrome Diarreico Neonatal, SDN), fueron de nueve para el primero de cinco para el segundo; existió un 20 porciento de mortalidad para este grupo. En el grupo II (FT + antibiótico) se encontró un aumento significativo (P<0.01) en la respuesta leucocitaria, dada por linfocitosis significativa (P<0.01); los días de recuperación fueron de ocho días con cuadro clinico respiratorio N y de cuatro con SDN, con mortalidad de 20 porciento. Los resultados obtenidos de laboratorio de los grupos II y III fueron semejantes, pues el aspecto clínico del grupo III y la recuperación de los animales fueron de cinco días para ambos cuadros clínicos y no hubo mortalidad.