Genetic correlations between wool traits and meat quality traits in Merino sheep.

Genetic correlations between 29 wool production and quality traits and 25 meat quality and nutritional value traits were estimated for Merino sheep from an Information Nucleus (IN). Genetic correlations among the meat quality and nutritional value traits are also reported. The IN comprised 8 flocks linked genetically and managed across a range of sheep production environments in Australia. The wool traits included over 5,000 yearling and 3,700 adult records for fleece weight, fiber diameter, staple length, staple strength, fiber diameter variation, scoured wool color, and visual scores for breech and body wrinkle. The meat quality traits were measured on samples from the and included over 1,200 records from progeny of over 170 sires for intramuscular fat (IMF), shear force of meat aged for 5 d (SF5), 24 h postmortem pH (pHLL; also measured in the , pHST), fresh and retail meat color and meat nutritional value traits such as iron and zinc levels, and long-chain omega-3 and omega-6 polyunsaturated fatty acid levels. Estimated heritabilities for IMF, SF5, pHLL, pHST, retail meat color lightness (), myoglobin, iron, zinc and across the range of long-chain fatty acids were 0.58 ± 0.11, 0.10 ± 0.09, 0.15 ± 0.07, 0.20 ± 0.10, 0.59 ± 0.15, 0.31 ± 0.09, 0.20 ± 0.09, 0.11 ± 0.09, and range of 0.00 (eicosapentaenoic, docosapentaenoic, and arachidonic acids) to 0.14 ± 0.07 (linoleic acid), respectively. The genetic correlations between the wool production and meat quality traits were low to negligible and indicate that wool breeding programs will have little or no effect on meat quality. There were moderately favorable genetic correlations between important yearling wool production traits and the omega-3 fatty acids that were reduced for corresponding adult wool production traits, but these correlations are unlikely to be important in wool/meat breeding programs because they have high SE, and the omega-3 traits have little or no genetic variance. Significant genetic correlations among the meat quality traits included IMF with SF5 (-0.76 ± 0.24), fresh meat color * (0.50 ± 0.18), and zinc (0.41 ± 0.19). Selection to increase IMF will improve meat tenderness and color which may address some of the issues with Merino meat quality. These estimated parameters allow Merino breeders to combine wool and meat objectives without compromising meat quality.

Genetic parameters for wool traits, live weight, and ultrasound carcass traits in Merino sheep.

Genetic correlations between 29 wool production and quality traits and live weight and ultrasound fat depth (FAT) and eye muscle depth (EMD) traits were estimated from the Information Nucleus (IN). The IN comprised 8 genetically linked flocks managed across a range of Australian sheep production environments. The data were from a maximum of 9,135 progeny born over 5 yr from 184 Merino sires and 4,614 Merino dams. The wool traits included records for yearling and adult fleece weight, fiber diameter (FD), staple length (SL), fiber diameter CV (FDCV), scoured color, and visual scores for breech and body wrinkle. We found high heritability for the major yearling wool production traits and some wool quality traits, whereas other wool quality traits, wool color, and visual traits were moderately heritable. The estimates of heritability for live weight generally increased with age as maternal effects declined. Estimates of heritability for the ultrasound traits were also higher when measured at yearling age rather than at postweaning age. The genetic correlations for fleece weight with live weights were positive (favorable) and moderate (approximately 0.5 ± 0.1), whereas those with FD were approximately 0.3 (unfavorable). The other wool traits had lower genetic correlations with the live weights. The genetic correlations for FAT and EMD with FD and SL were positive and low, with FDCV low to moderate negative, but variable with wool weight and negligible for the other wool traits. The genetic correlations for FAT and EMD with postweaning weight were positive and high (0.61 ± 0.18 to 0.75 ± 0.14) but were generally moderate with weights at other ages. Selection for increased live weight will result in a moderate correlated increase in wool weight as well as favorable reductions in breech cover and wrinkle, along with some unfavorable increases in FD and wool yellowness but little impact on other wool traits. The ultrasound meat traits, FAT and EMD, were highly positively genetically correlated (0.8), and selection to increase them would result in a small unfavorable correlated increase in FD, moderately favorable reductions in breech cover and wrinkle, but equivocal or negligible changes in other wool traits. The estimated parameters provide the basis for calculation of more accurate Australian Sheep Breeding Values and selection indexes that combine wool and meat objectives in Merino breeding programs.

Genetic correlations between wool traits and carcass traits in Merino sheep.

Genetic correlations between 29 wool production and quality traits and 14 whole carcass measures and carcass component traits were estimated from the Information Nucleus of 8 flocks managed across a range of Australian sheep production environments and genetically linked. Wool data were from over 5,000 Merino progeny born over 5 yr, whereas carcass data were from over 1,200 wether progeny of over 176 sires, slaughtered at about 21 kg carcass weight, on average. Wool traits included yearling and adult records for wool weight, fiber diameter, fiber diameter variation, staple strength, scoured color, and visual scores for breech and body wrinkle. Whole carcass measures included HCW, dressing percentage (DP), and various measures of fat depth and eye muscle dimensions. Carcass components were obtained by dissection, and lean meat yield (LMY) was predicted. Heritability estimates for whole carcass measures ranged from 0.12 ± 0.08 to 0.35 ± 0.10 and ranged from 0.17 ± 0.10 to 0.46 ± 0.10 for carcass dissection traits, with no evidence of important genotype × environment interactions. Genetic correlations indicated that selection for increased clean wool weight will result in reduced carcass fat (-0.17 to -0.34) and DP (-0.48 ± 0.15), with little effect on carcass muscle. Selection for lower fiber diameter will reduce HCW (-0.48 ± 0.15) as well as carcass fat (0.14 to 0.27) and muscle (0.21 to 0.50). There were high genetic correlations between live animal measures of fat and muscle depth and the carcass traits (generally greater than 0.5 in size). Selection to increase HCW (and DP) will result in sheep with fewer wrinkles on the body (-0.57 ± 0.10) and barer breeches (-0.74 ± 0.12, favorable), with minor deterioration in scoured wool color (reduced brightness and increased yellowness). Selection for reduced fat will also result in sheep with fewer body wrinkles (-0.42 to -0.79). Increasing LMY in Merinos through selection would result in a large reduction in carcass fat and DP (-0.66 to -0.84), with a smaller increase in carcass muscle and some increase in wool weight and wrinkles. Although no major antagonisms are apparent between the wool and carcass traits, developing selection indexes for dual-purpose wool and meat breeding objectives will require accurate estimates of genetic parameters to ensure that unfavorable relationships are suitably considered. The findings will aid development of dual-purpose wool and meat breeding objectives.

Different epigenetic states define syncytiotrophoblast and cytotrophoblast nuclei in the trophoblast of the human placenta.

INTRODUCTION: The syncytiotrophoblast (STB) epithelial covering of the villous tree in the human placenta is a multi-nucleated syncytium that is sustained by continuous incorporation of differentiating cytotrophoblast (CTB) cells. STB nuclei display a variety of morphologies, but are generally more condensed in comparison to CTB nuclei. Here, we consider whether this condensation is a feature of epigenetic regulation of chromatin structure. METHODS: Semi-quantitative immunohistochemical investigations of a panel of histone modifications were performed to determine the relative proportions in CTB and STB nuclear populations. We also investigated the patterns of DNA methylation and distribution of DNA methyltransferases enzymes in these populations. RESULTS: Unexpectedly DNA methylation, and H3K9me3 and H3K27me3, which are modifications associated with heterochromatin, are present at lower levels in STB nuclei compared to CTB, despite the intensive condensation in the former nuclear population and the progenitor state of the latter. By contrast, STB nuclei are enriched for H4K20me3, which is also associated with repressive states. 5'hydroxymethylcytosine immunoreactivity is higher in STB, with intense staining observed in the highly condensed nuclei within syncytial knots. DISCUSSION: Cell-type specific epigenetic states exist within the trophoblast populations potentially regulating their different functions and developmental properties and suggesting non-canonical epigenetic states associated with the properties of these cells.

IFPA Meeting 2011 workshop report III: Placental immunology; epigenetic and microRNA-dependent gene regulation; comparative placentation; trophoblast differentiation; stem cells.

Workshops are an important part of the IFPA annual meeting as they allow for discussion of specialised topics. At IFPA meeting 2011 there were twelve themed workshops, five of which are summarized in this report. These workshops related to various aspects of placental biology: 1) immunology; 2) epigenetics; 3) comparative placentation; 4) trophoblast differentiation; 5) stem cells.

A quantitative analysis of transcriptionally active syncytiotrophoblast nuclei across human gestation.

The syncytiotrophoblast (STB) epithelial covering of the human placenta is a unique terminally differentiated, multi-nucleated syncytium. No mitotic bodies are observed in the STB, which is sustained by continuous fusion of underlying cytotrophoblast cells (CTB). As a result, STB nuclei are of different ages. Morphologically, they display varying degrees of chromatin compaction, suggesting progressive maturational changes. Until recently, it was thought that STB nuclei were transcriptionally inactive, with all the mRNAs required by the syncytium being incorporated upon fusion of CTB. However, recent research has shown the presence of the active form of RNA polymerase II (RNA Pol II) in some STB nuclei. In this study, we confirm the presence of transcriptional activity in STB nuclei by demonstrating immunoreactivity for a transcription factor and an RNA polymerase I (RNA Pol I) co-factor, phospho-cAMP response element-binding protein and phospho-upstream binding factor, respectively. We also show, through immunoco-localisation studies, that a proportion of STB nuclei are both RNA Pol I and II transcriptionally active. Finally, we quantify the numerical densities of nuclei immunopositive and immunonegative for RNA Pol II in the STB of normal placentas of 11-39 weeks gestational age using an unbiased stereological counting tool, the physical disector. These data were combined with estimates of the volume of trophoblast to calculate total numbers of both types of nuclei at each gestational age. We found no correlation between gestational age and the numerical density of RNA Pol II-positive nuclei in the villous trophoblast (r = 0.39, P > 0.05). As the number of STB nuclei increases exponentially during gestation, we conclude that the number of transcriptionally active nuclei increases in proportion to trophoblast volume. The ratio of active to inactive nuclei remains constant at 3.9:1. These findings confirm that the majority of STB nuclei have intrinsic transcriptional activity, and that the STB is not dependent on CTB fusion for the provision of transcripts.

Genetic parameters for carcass and meat quality traits and their relationships to liveweight and wool production in hogget Merino rams.

Genetic parameters for carcass and meat quality traits of about 18-month-old Merino rams (n = 5870), the progeny of 543 sires from three research resource flocks, were estimated. The estimates of heritability for hot carcass weight (HCW) and the various fat and muscle dimension measurements were moderate and ranged from 0.20 to 0.37. The brightness of meat (colour L*, 0.18 +/- 0.03 standard error) and meat pH (0.22 +/- 0.03) also had moderate estimates of heritability, although meat relative redness (colour a*, 0.10 +/- 0.03) and relative yellowness (colour b*, 0.10 +/- 0.03) were lower. Heritability estimates for live weights were moderate and ranged from 0.29 to 0.41 with significant permanent maternal environmental effects (0.13 to 0.10). The heritability estimates for the hogget wool traits were moderate to high and ranged from 0.27 to 0.60. The ultrasound measurements of fat depth (FATUS) and eye muscle depth (EMDUS) on live animals were highly genetically correlated with the corresponding carcass measurements (0.69 +/- 0.09 FATC and 0.77 +/- 0.07 EMD). Carcass tissue depth (FATGR) had moderate to low genetic correlations with carcass muscle measurements [0.18 +/- 0.10 EMD and 0.05 +/- 0.10 eye muscle area (EMA)], while those with FATC were negative. The genetic correlation between EMD and eye muscle width (EMW) was 0.41 +/- 0.08, while EMA was highly correlated with EMD (0.89 +/- 0.0) and EMW (0.78 +/- 0.04). The genetic correlations for muscle colour with muscle measurements were moderately negative, while those with fat measurements were close to zero. Meat pH was positively correlated with muscle measurements (0.14 to 0.17) and negatively correlated with fat measurements (-0.06 to -0.18). EMDUS also showed a similar pattern of correlations to EMD with meat quality indicator traits, although FATUS had positive correlations with these traits which were generally smaller than their standard error. The genetic correlations among the meat colour traits were high and positive while those with meat pH were high and negative, which were all in the favourable direction. Generally, phenotypic correlations were similar or slightly lower than the corresponding genetic correlations. There were generally small to moderate negative genetic correlations between clean fleece weight (CFW) and carcass fat traits while those with muscle traits were close to zero. As the Merino is already a relatively lean breed, this implies that particular attention should be given to this relationship in Merino breeding programmes to prevent the reduction of fat reserves as a correlated response to selection for increased fleece weight. The ultrasound scan traits generally showed a similar pattern to the corresponding carcass fat and muscle traits. There was a small unfavourable genetic correlation between CFW and meat pH (0.19 +/- 0.07).

Genetic variation of plasma insulin-like growth factor-1 in young crossbred ewes and its relationship with their maintenance feed intake at maturity and production traits.

The genetic variation of plasma IGF-I in crossbred ewe lambs postweaning was evaluated together with its potential use as a physiological marker for selection in meat sheep. Genetic variation for IGF-I was analyzed among 1,246 young crossbred ewes that were the progeny of 30 sires from various maternal breeds and Merino dams. The estimate of heritability of IGF-I was 0.28 +/- 0.10, with sire breed not being significant. Genetic correlations were estimated between IGF-I and performance traits of the ewes, including feed intake, growth, body composition, wool, and reproduction over 3 matings. Although the genetic correlations had high standard errors because of the limited size of the data set, the correlation between IGF-I and grazing feed intake of the mature ewes at maintenance was positive (0.32 +/- 0.31). The genetic correlations of IGF-I with other traits ranged from positive and low to moderate for growth (0.05 to 0.36), positive for ultrasound eye muscle depth (0.15), and negative for ultrasound fat depth (-0.12) in the mature ewes, and close to zero for the wool traits. The genetic correlation between IGF-I and the average number of lambs born per ewe mated was negative (-0.18), whereas that for the average number of lambs weaned per ewe mated was positive (0.10). The parameters indicated that genetic variation exists for IGF-I in sheep, and selection for low IGF-I in young ewes may result in some reduction in feed intake and improvement in maintenance efficiency of mature ewes under grazing, with little impact on other production traits. However, the genetic correlations had high standard errors, and more precise estimates of these parameters are required for genetic evaluation and to predict with confidence the outcome of breeding programs.

Reproductive performance and genetic parameters in first cross ewes from different maternal genotypes.

The reproduction of 2,846 crossbreed ewes with 7,899 records is reported. The ewes were progeny of mainly Merino dams and 91 sires from several maternal sire breeds including Border Leicester, East Friesian, Finnsheep, Coopworth, Corriedale, Booroola Leicester, and several others. There were 3 cohorts of ewes at each of 3 sites that were bred naturally to meat-type rams for each of 3 yr to evaluate reproduction and lamb production. At 2 sites, the ewes were mated in the autumn, first at 7 mo of age, and at 2 sites the ewes were mated in the spring, first at 14 or 17 mo of age. The cohorts of ewes and sites were genetically linked by 3 common sires. Mixed linear models were used to analyze ultrasound scanned pregnancy rate, fetal number, fertility (ewes lambing), litter size, lamb survival, number of lambs born (NLBj), number of lambs weaned (NLWj), and total weight of lamb weaned (TWWj) per ewe bred. Fixed effects included sire breed (1 to 10), environment (1 to 4, site and season of breeding: autumn, spring), breeding (1 to 3), cohort (1 to 3), and their interactions. The REML procedures were used to estimate (co)variance components. Ewe sire breed effects were significant (P < 0.01) for all the reproductive traits and breed means ranged from 0.75 to 0.96 for fertility, 1.22 to 2.08 for litter size, 0.70 to 0.90 for lamb survival, 0.99 to 1.66 for NLBj, 0.87 to 1.26 for NLWj, and 22.9 to 33.8 kg for TWWj, with the ranking of sire breeds varying for different traits. For all traits except lamb survival, the contrast between breeding 1 vs. 2 and 3 was considerably greater than the contrast between breeding 2 vs. 3, with significant environment x breeding interactions (P < 0.01). Estimates of heritability for the components of reproduction ranged from 0.03 +/- 0.02 for lamb survival to 0.19 +/- 0.05 for litter size, and those for the composite traits were 0.17 +/- 0.04 for NLBj, 0.13 +/- 0.04 for NLWj, and 0.17 +/- 0.04 for TWWj, with repeatability ranging from 0.10 to 0.19. Genetic and phenotypic correlations among the traits are reported. The significant variation among sire breeds of the crossbred ewes can be used to improve reproduction, although there was a change in the rank of the breeds for the various traits. There was considerable overlap between the breeds, and additional improvement could be achieved by exploiting the genetic variation between sires within breeds for all the ewe reproductive traits.

Genetic correlations between ewe reproduction and carcass and meat quality traits in Merino sheep.

Genetic correlations between reproduction traits in ewes and carcass and meat quality traits in Merino rams were obtained using restricted maximum likelihood procedures. The carcass data were from 5870 Merino rams slaughtered at approximately 18 months of age that were the progeny of 543 sires from three research resource flocks over 7 years. The carcass traits included ultrasound scan fat and eye muscle depth (EMDUS) measured on live animals, dressing percentage and carcass tissue depth (at the GR site FATGR and C site FATC), eye muscle depth, width and area and the meat quality indicator traits of muscle final pH and colour (L*, a*, b*). The reproduction data consisted of 13 464 ewe joining records for number of lambs born and weaned and 9015 records for LS. The genetic correlations between reproduction and fat measurements were negative (range -0.06 +/- 0.12 to -0.37 +/- 0.12), with smaller correlations for live measurement than carcass traits. There were small favourable genetic correlations between reproduction traits and muscle depth in live rams (EMDUS, 0.10 +/- 0.12 to 0.20 +/- 0.12), although those with carcass muscle traits were close to zero. The reproduction traits were independent of meat colour L* (relative brightness), but tended to be favourably correlated with meat colour a* (relative redness, 0.12 +/- 0.17 to 0.19 +/- 0.16). There was a tendency for meat final pH to have small negative favourable genetic correlations with reproduction traits (0.05 +/- 0.11 to -0.17 +/- 0.12). This study indicates that there is no antagonism between reproduction traits and carcass and meat quality indicator traits, with scope for joint improvement of reproduction, carcass and meat quality traits in Merino sheep.

Genetic correlations among and between wool, growth and reproduction traits in Merino sheep.

Data from seven research resource flocks across Australia were combined to provide accurate estimates of genetic correlations among production traits in Merino sheep. The flocks represented contemporary Australian Merino fine, medium and broad wool strains over the past 30 years. Over 110,000 records were available for analysis for each of the major wool traits, and 50,000 records for reproduction and growth traits with over 2700 sires and 25,000 dams. Individual models developed from the single trait analyses were extended to the various combinations of two-trait models to obtain genetic correlations among six wool traits [clean fleece weight (CFW), greasy fleece weight, fibre diameter (FD), yield, coefficient of variation of fibre diameter and standard deviation of fibre diameter], four growth traits [birth weight, weaning weight, yearling weight (YWT), and hogget weight] and four reproduction traits [fertility, litter size, lambs born per ewe joined, lambs weaned per ewe joined (LW/EJ)]. This study has provided for the first time a comprehensive matrix of genetic correlations among these 14 wool, growth and reproduction traits. The large size of the data set has also provided estimates with very low standard errors. A moderate positive genetic correlation was observed between CFW and FD (0.29 +/- 0.02). YWT was positively correlated with CFW (0.23 +/- 0.04), FD (0.17 +/- 0.04) and LWEJ (0.58 +/- 0.06), while LW/EJ was negatively correlated with CFW (-0.26 +/- 0.05) and positively correlated with FD (0.06 +/- 0.04) and LS (0.68 +/- 0.04). These genetic correlations, together with the estimates of heritability and other parameters provide the basis for more accurate prediction of outcomes in complex sheep-breeding programmes designed to improve several traits.

Diverse lamb genotypes. 3. Eating quality and the relationship between its objective measurement and sensory assessment.

The effect of genotype on eating quality was evaluated on m. Longissimus thoracis et lumborum (LTL) muscle of 60 lambs. The lambs were sired by a selection of Texel (T), Poll Dorset (PD), Border Leicester (BL) and Merino (M) rams, crossed with Border Leicester x Merino (BLM) and Merino (M) ewes giving six genotypes (TxBLM, PDxBLM, TxM, PDxM, BLxM and MxM). The relationships between sensory panel assessment of eating quality attributes and pH, cooking loss and shear force were also investigated. No significant differences were observed between genotypes for panel assessment of tenderness, juiciness, aroma liking, aroma strength, flavour liking, overall acceptability and rating. MxM lambs had a significantly (P<0.05) higher flavour strength than BLxM lambs. pH was a poor indicator of any eating quality attributes, except aroma strength (r=0.3, P<0.05). Warner Bratzler shear force value (WB) and tenderness showed a significant (P<0.001) negative correlation (-0.7). Tenderness, flavour and juiciness were the most important sensory attributes, explaining 86.5% of the total variation in overall acceptability.

Diverse lamb genotypes 4. Predicting the yield of saleable meat and high value trimmed cuts from carcass measurements.

To determine how current predictors of carcass and cut yield perform in the Australian lamb industry a large study was undertaken using diverse genotypes. Carcass measurements and yield data were obtained for 591 lambs representing two sexes (cryptorchids, ewes). The lambs were sired by a selection of Texel (T), Poll Dorset (PD), Border Leicester (BL) and Merino (M) rams crossed with Border Leicester×Merino (BLM) and Merino (M) ewes giving six genotypes (T×BLM, PD×BLM, T×M, PD×M, BL×M, M×M). The percentage yield of saleable meat, hindquarter, loin and a range of trimmed cuts were predicted using models based on hot carcass weight, EUROP conformation score, m. longissimus thoracis et lumborum area (LL area) in combination with three different measures of carcass fatness: (1) the depth of muscle and fat tissue from the surface of the carcass to the lateral surface of 12th rib, 110 mm from the midline (GR); (2) fat depth over the LL at the deepest part of the muscle (FATC); and (3) fat depth at the 5th rib, 110 mm from the midline (FAT5). FAT5 was identified as the most accurate and robust (stable) fat measurement for the prediction of saleable meat yield in a production system based on diverse genotypes. Using GR as a fatness measurement led to overestimation of fatness level and underestimation of yield in T sired lambs. Conformation score after carcass weight and any of the fat measurements improved (P<0.01) the prediction of the yield of saleable meat, hindquarter, loin and all trimmed lamb cuts with the exception of the ribloin (rack). LL area added significantly (P<0.01) to the prediction of the yield of saleable meat, hindquarter and trimmed lamb cuts, but not for the midloin and neck fillet at a constant carcass weight and fatness. Large proportions of the variation in all yields were unaccounted for, even after the inclusion of either conformation or LL area. Predictions based on carcass weight and any of the three different fat measurements were not very accurate. Although the addition of LL area as a predictor significantly (P<0.01) increased the accuracy of the predictions the results suggest that the current description system for lamb carcases in Australia needs to be re-examined.

The viability of transferred sheep embryos after long-term cryopreservation.

The survival to term of 414 sheep embryos, thawed and transferred after conventional cryopreservation and storage for 13 years, was evaluated. A concurrent experiment involving the transfer of vitrified embryos to 91 ewes and artificial insemination of 51 ewes with frozen-thawed semen from sires of the long-term cryopreserved embryos provided forms of control treatments. The donor ewes had a mean ovulation rate of 10.9, and 7.1 embryos per ewe were cryopreserved. Each recipient ewe received two embryos and pregnancy was assessed at Day 18, Day 54 and term. The pregnancy rate was lower in the long-term embryo group than the artificial insemination group at Day 18 (P<0.01) and Day 54 (P<0.05), although the difference at term (31% v. 49%) was not significant, with the vitrified embryo group being similar to the long-term group. Embryo survival to birth was 21%, with the majority of loss (80%) occurring by Day 18. The later stage of development and higher grade of transferred embryos and the older age of donor ewes resulted in a significantly higher (P<0.01) pregnancy rate at Day 54 and term, and percentage of lambs born and weaned. Other effects of donor ewes (genotype, superovulation treatment, number of ovulations and embryos cryopreserved) were not significant. Implications for the design of genetic evaluation and germplasm conservation programmes using embryo cryopreservation technology are discussed.

Diverse lamb genotypes-1. Yield of saleable cuts and meat in the carcass and the prediction of yield.

Carcass measurements and composition data were obtained for 591 lambs representing two sexes (307 cryptorchid males, 284 ewes). The lambs were sired by a selection of Texel (T), Poll Dorset (PD), Border Leicester (BL) and Merino (M) rams, crossed with Border Leicester × Merino (BLM) and Merino (M) ewes giving 6 genotypes (T × ELM, PD × BLM, T × M, PD × M, BL × M, M × M). m × M carcasses were significantly (p < 0.05) leaner than all other genotypes as measured by GR at a common weight, except for ewe carcasses where they were not significantly leaner than T × M carcasses. There was no difference in GR between Texel and Poll Dorset sired carcasses. The BL × M and PD × BLM carcasses were the fattest at the 5th rib. M × M lambs produced the lightest carcasses in each sex. Genotype differences were found for conformation using the EUROP scoring system. M×M lambs had a greater proportion (p < 0.001) of poorer scores particularly compared to PD × BLM and T × BLM carcasses. Analysis of Texel and Poll Dorset sired carcasses showed that the Texel cross had a better conformation than Poll Dorset cross amongst both sexes. BLM dams produced carcasses with a superior conformation compared to Merino dams (p < 0.001) in both sexes. BL × M carcasses had a significantly (p < 0.05) smaller M. longissimus thoracis et lumborum area (LL area) than other genotypes, except for M × M ewe carcasses. Texel sired ewe carcasses had a larger LL area (p < 0.05) than those sired by Poll Dorsets, but there was no significant difference for the heavier cryptorchid carcasses. Saleable meat yield for the BL × M carcasses of both sexes was significantly less (p < 0.001) than all other genotypes. M × M carcasses of both sexes produced similar amounts of saleable product to second cross carcasses with the T × M carcasses being superior, resulting in a significant sire breed effect. Texel sired carcasses produced more product than Poll Dorset carcasses. Year had a significant (p < 0.05) effect on all carcass measures and saleable meat yield. Genotype had a significant (p < 0.05) effect on the proportion of each cut except for the neck from cryptorchids and neck fillet from ewes. Texel sired genotypes had significantly (p < 0.05) heavier weights for the hindleg and forequarter cuts than those sired by Poll Dorsets. For the BL × M and m × M genotypes, some of the hindleg cuts, such as the topside and silverside weighed less than other genotypes. The boneless loins from the Poll Dorset genotypes were the heaviest, resulting in a significant (p < 0.05) sire breed effect, with all other genotypes being similar. Models were developed to estimate the yield of cuts and saleable meat using hot carcass weight, GR and other variables. The amount of variation in yield explained by hot carcass weight and GR ranged from r(2) = 0.06 to 0.23. Inclusion of LL area increased the r(2) values up to 0.39. Inclusion of both genotype and year significantly (p < 0.05) improved the prediction of yield, with year contributing the most. The addition of sex had no effect on the prediction of yield.

Diverse lamb genotypes-2. Meat pH, colour and tenderness.

The effect of genotype on meat ultimate pH, colour and tenderness was studied in 3 experiments involving 436 lambs. The lambs were sired by a selection of Texel (T), Poll Dorset (PD), Border Leicester (BL) and Merino (M) rams, crossed with Border Leicester × Merino (BLM) and Merino (M) ewes giving 6 genotypes (T × BLM, PD × BLM, T × M, PD × M, BLM and M × M). In experiment 1, pH data were obtained for 4 muscles (M. Semimembranosus (SM), M. Biceps femoris M. Semitendinosus and M. Longissimius thoracis et lumborum (LL)) from 198 carcasses representing two sexes (104 cryptorchid males, 94 ewes). There were significant differences for pH between genotypes in both sex groups. BL × M and M × M lambs tended to have higher pH values than the other genotypes. In experiment 2, meat pH, colour using a chromameter, and tenderness data were obtained for 186 lambs representing two sexes (101 cryptorchid males, 85 ewes). There were few significant differences between genotypes for the various meat quality traits amongst the ewes. The PD × BLM ewes had a significantly lower (p < 0.05) pH than the BL × M ewes for the LL and SM muscles. The BL × M crypt orchids had a higher LL muscle pH (p < 0.05) than the PD × BLM cryptorchids with other genotypes being intermediate. There were no significant differences between the genotypes for muscle shear force values. Within experiment 2, LL samples were taken from an additional 52 cryptorchid carcasses these being from 3 specific Poll Dorset sires (experiment 3). One of these sires (sire 3) was suspected of carrying a gene for increased muscling. Sire had a significant effect (p < 0.05) on shear force values, cooking loss and L(∗) colour values, but not a(∗) or b(∗) colour values or pH. Sire 3 progeny had significantly (p < 0.001) tougher LL muscles than those from the other 2 sires. Sire 3 also had the lowest L(∗) values, but was intermediate for cooking loss. There was no significant sire effect on LL area at the same carcass weight, but boneless loins from the progeny of Sire 3 were significantly (p < 0.05) heavier. In a fourth experiment, meat colour was measured at three sites (LL, diaphragm and M. Obliquus externus abdominis) on 86 cryptorchid carcasses 24 hr post-mortem. There was little association between L(∗), a(∗) and b(∗) values measured at the different sites.

Differences in composition, muscularity, muscle:bone ratio and cut dimensions between six lamb genotypes.

Carcass measurements and composition data were obtained for 198 lambs representing two sexes (104 cryptorchids, 94 ewes). Dorsal images of 166 of the carcasses were obtained on the slaughter chain using a video camera. The lambs were sired by a selection of Poll Dorset (PD), Texel (T), Border Leicester (BL) and Merino (M) rams, born to Border Leicester × Merino (BLM) and Merino (M) ewes giving 6 genotypes (PD × BLM, T × BLM, PD × M, T × M, BL × M, M × M). From the right hindleg and chump, the following muscles were dissected and weighed: semimembranosus, adductor femoris, semitendinosus, biceps femoris, and quadriceps femoris. The femur was weighed, the length measured and a muscularity value calculated as described by Purchas, et al. (1991, Meat Sci., 30, 181). Merino cryptorchid lambs were significantly (P < 0.05) leaner, as measured by GR tissue depth (for explanation, see MATERIALS AND METHODS) adjusted to a mean carcass weight of 24.2 kg, than BL × M lambs, but there was no difference between genotypes within the ewe group (mean carcass weight of 17.1 kg). Genotype differences were found for conformation using the EUROP scoring system. Merino lambs had a greater proportion (P < 0.001) of poorer scores, particularly compared to PD × BLM and T × BLM carcasses. The BL × M carcasses had a significantly (P < 0.05) smaller m. longissimus thoracis et lumborum area than other genotypes with no difference between carcasses of T and PD sires. Muscularity values for the BL × M and M lambs were significantly (P < 0.05) lower than the other crosses but not different from each other. Carcasses from T sires had significantly (P < 0.05) higher muscularity values than those from PD sires. A negative association (r = -0.56) was found between EUROP scores and muscularity when carcass differences were adjusted for, but significant differences between genotypes for muscularity were not necessarily carried through to differences in EUROP conformation score. BL × M carcasses had the lowest muscle:bone ratio and T × M the highest with M carcasses having similar ratios to carcasses from PD-sired lambs. Only small differences were found between genotypes for composition of the hindleg, the exception being BL × M carcasses of both sexes which were significantly (P < 0.05) fatter than T × BLM, T × M and PD × M carcasses. Carcasses from T sires had significantly (P < 0.05) more muscle in the hindleg than carcasses from PD sires, with the differences being greater in the heavier cryptorchids (1.8%) than the ewes (1.1%). There was no consistent effect of genotype on the cross-sectional areas of the round and topside cuts within either sex. Differences between genotypes for the proportion of the carcass attributed to the round were small and only for BL × M ewe carcasses was this cut significantly lighter (P < 0.05) than from all other genotypes, except the PD × BLM carcasses. This trend was similar for the topside and silverside cuts. A moderate correlation (r = 0.46; P < 0.001) was found between muscularity values determined by dissection and those predicted using the equations of Hopkins et al. (1996, Proc. Aust. Soc. Anim. Prod., 21, 181) based on carcass width measurements taken from video images.

Seasonality of ovulation and estrus, and the ram effect in poll dorset ewes.

The effects of month on the proportion of Poll Dorset ewes expressing estrus and ovulating and on their ovulation rate when continually exposed to vasectomised rams and the effects of isolating the ewes from rams in winter/early spring were examined during 15 months in New South Wales, Australia. The percentage of ewes ovulating and their ovulation rate varied from 23% and 1.10 in November to an average of 99% and 1.85 in April and June, and the proportion of ewes expressing estrus followed a similar trend. Some ewes (6.8%) ovulated throughout the 15 months, and the average breeding season was 294 (SE = 6.1) days. Isolating ewes from rams in late winter/early spring significantly decreased the proportion of ewes ovulating from September to November, increased the proportion in December, but did not significantly alter ovulation rate. The variability in reproductive measures during spring offers scope for selection; with the ram effect, improvements in spring joining results from Dorset ewes are possible.

Lamb production and its components in pure breeds and composite lines. II. Breed effects and heterosis.

Ewe performance was compared for five pure breeds of sheep (Finnsheep, F; Rambouillet, R; Dorset, D; Targhee, T; Suffolk, S) and the crosses in development of two maternal composite lines (C1 = 1/2F1/4R1/4D and C2 = 1/2F1/4T1/4S). The data involved 10,959 breeding season records of 4,219 ewes by 412 sires. Ewe production and the components (fertility, litter size, neonatal and preweaning lamb survival and mean lamb weaning weight) were adjusted for age and standardized across season of lambing and years. The D and F ewes produced more weight of lamb/ewe exposed than R, S and T ewes because of higher D and F fertility, higher D lamb survival and larger F litters. First cross and inter se generations of C1 and C2 ewes averaged 17 to 27% higher fertility than the parental mean. Litter size averaged about one lamb higher for F than for other pure breeds, but only slightly higher for C1 and C2 than for the mean of F and other breeds. Lower neonatal survival for F than for other breeds and crosses was associated with the larger F litters and with 2 to 8% positive heterosis in the crosses. Preweaning survival of suckled and nursery lambs was low for F and S and positive heterosis ranged from 9 to 19% in crosses. Mean lamb weaning weights were highest for S, lowest for F, with little heterosis in crosses. Crossbred ewes reared .3 to .4 more lambs than mean for parental pure breeds. Heterosis in C1 and C2 ranged from 11 to 28% for lambs born, 27 to 43% for lambs weaned and 29 to 44% for weight of lamb weaned/ewe exposed. Decline in heterosis with inter se mating of crosses was no greater than expected from the reduction in predicted heterozygosity.

Lamb production and its components in pure breeds and composite lines. I. Seasonal and other environmental effects.

Season of lambing and other environmental effects on ewe performance were analyzed for purebred Finnsheep (F), Rambouillet (R), Dorset (D), Targhee (T) and Suffolk (S) and the generations of crosses in development of two maternal composite lines (1/2F1/4R1/4D) and (1/2F1/4T1/4S) in accelerated (January, May and September) or annual April lambing. The data involved 10,959 ewe breeding season records for 4,219 ewes of 412 sire families over 4 yr. Various measures of ewe productivity and its components (fertility, litter size, neonatal and preweaning survival and weaning weight) were analyzed. Fertility was higher for annual April than for accelerated May or January lambing and was sharply lower for September lambing. Fertility of F and F-cross ewes was significantly higher for May and lower for January lambings relative to R and D ewes. Litter size also was higher in annual April (1.9) than in January (1.8) or May (1.7) and September (1.4). Neonatal and preweaning survival was higher in September when litter size was smaller. Mean weaning weights were depressed in the larger April and May litters. Thus, weight of lambs weaned/ewe exposed was higher for annual April than for May and January lambing and was very low for September lambing. Hormone treatment of ewes for September lambing increased fertility from 16 to 44% and litter size from 1.6 to 1.8. Short lambing interval (8 mo vs 12+ mo) reduced mean ewe fertility by five percentage points, and most for the January lambing of Finnsheep ewes.