Does high-intensity running to fatigue influence lower limb injury risk?

OBJECTIVES: The aim of this study was to quantify changes in peak bending moments at the distal tibia, peak patellofemoral joint contact forces and peak Achilles tendon forces during a high-intensity run to fatigue at middle-distance speed. DESIGN: Observational study. METHODS: 16 high-level runners (7 female) ran on a treadmill at the final speed achieved during a preceding maximum oxygen uptake test until failure (~3 min). Three-dimensional kinetics and kinematics were used to derive and compare tibial bending moments, patellofemoral joint contact forces and Achilles tendon forces at the start, 33 %, 67 % and the end of the run. RESULTS: Average running speed was 5.7 (0.4) m·s-1. There was a decrease in peak tibial bending moments (-6.8 %, p = 0.004) from the start to the end of the run, driven by a decrease in peak bending moments due to muscular forces (-6.5 %, p = 0.001), whilst there was no difference in peak bending moments due to joint reaction forces. There was an increase in peak patellofemoral joint forces (+8.9 %, p = 0.026) from the start to the end of the run, but a decrease in peak Achilles tendon forces (-9.1 %, p < 0.001). CONCLUSIONS: Running at a fixed, high-intensity speed to failure led to reduced tibial bending moments and Achilles tendon forces, and increased patellofemoral joint forces. Thus, the altered neuromechanics of high-intensity running to fatigue may increase patellofemoral joint injury risk, but may not be a mechanism for tibial or Achilles tendon overuse injury development.

Muscle and tendon morphology of a world strongman and deadlift champion.

This study compared the muscle and tendon morphology of an extraordinarily strong individual, a world's strongest man (WSM) and deadlift champion, to that of various other athletic, trained and untrained populations. The WSM completed: (1) 3.0-T MRI scans, to determine the volume of 22 individual lower limb muscles, 5 functional muscle groups, patellar tendon (PT) cross-sectional area (CSA), and PT moment arm; (2) countermovement jumps (CMJ) and isometric mid-thigh pull (IMTP) contractions. The WSM was compared to previously assessed groups from our laboratory (muscle and tendon) and the wider research literature (CMJ and IMTP). The WSM's CMJ peak power (9,866 W), gross (9,171 N) and net (7,480 N) IMTP peak force were higher than any previously published values. The WSM's overall measured leg muscle volume was ~twice that of untrained controls (+96%) but with pronounced anatomical variability in the extent of muscular development. The plantar flexor group (+120%) and the guy rope muscles (sartorius, gracilis, and semitendinosus +140 to +202%), that stabilise the pelvis and femur, demonstrated the largest differences relative to untrained controls. The WSM's pronounced quadriceps size (≥2-fold vs. untrained) was accompanied by modest PT moment arm differences, and notably was not matched by an equivalent difference in PT CSA (+30%). These results provide novel insight into the musculotendinous characteristics of an extraordinarily strong individual, that may be towards the upper limit of human variation, such that the WSM's very pronounced lower limb muscularity also exhibited distinct anatomical variability, and with muscle size largely uncoupled from tendon size.

How Humans Run Faster: The Neuromechanical Contributions of Functional Muscle Groups to Running at Different Speeds.

How the neuromechanics of the lower limb functional muscle groups change with running speed remains to be fully elucidated, with implications for our understanding of human locomotion, conditioning, and injury prevention. This study compared the neuromechanics (ground reaction and joint kinetics, kinematics and muscle activity) of middle-distance athletes running on an instrumented treadmill at six wide-ranging speeds (2.78-8.33 m·s-1). Ground reaction forces and kinematics were analyzed using inverse dynamics to calculate flexor and extensor joint torques, and positive and negative work done by these torques. Contributions of each functional muscle group to the total positive and negative work done by the limb during stance, swing, and the whole stride were quantified. During stance, the ankle plantar flexors were the major energy generator and absorber (>60%) at all speeds, but their contribution to whole stride energy generation and absorption declined with speed. Positive work by the hip extensors rose superlinearly with speed during stance (3-fold) and especially during swing (12-fold), becoming the biggest energy generator across the whole stride at >5 m·s-1. Knee flexor and extensor negative work also rose superlinearly with speed during swing, with the knee flexors becoming the greatest energy absorber over the whole stride at >7.22 m·s-1. Across speeds, plantar flexor peak moment and positive work accounted for 97% and 96% of the variance in step length, and swing hip extension peak moment and positive work accounted for 98% and 99% of the variance in step frequency. There were pronounced speed, phase (stance/swing), and work (positive/negative) dependent contributions of the different functional muscle groups during running, with extensive implications for conditioning and injury prevention.

Hamstrings Hypertrophy Is Specific to the Training Exercise: Nordic Hamstring versus Lengthened State Eccentric Training.

INTRODUCTION: The hamstring muscles play a crucial role in sprint running but are also highly susceptible to strain injuries, particularly within the biceps femoris long head (BFlh). This study compared the adaptations in muscle size and strength of the knee flexors, as well as BFlh muscle and aponeurosis size, after two eccentrically focused knee flexion training regimes: Nordic hamstring training (NHT) vs lengthened state eccentric training (LSET, isoinertial weight stack resistance in an accentuated hip-flexed position) vs habitual activity (no training controls: CON). METHODS: Forty-two healthy young males completed 34 sessions of NHT or LSET over 12 wk or served as CON ( n = 14/group). Magnetic resonance imaging-measured muscle volume of seven individual knee flexors and BFlh aponeurosis area, and maximum knee flexion torque during eccentric, concentric, and isometric contractions were assessed pre- and post-training. RESULTS: LSET induced greater increases in hamstrings (+18% vs +11%) and BFlh (+19% vs +5%) muscle volumes and BFlh aponeurosis area (+9% vs +3%) than NHT (all P ≤ 0.001), with no changes after CON. There were distinctly different patterns of hypertrophy between the two training regimes, largely due to the functional role of the muscles; LSET was more effective for increasing the size of knee flexors that also extend the hip (2.2-fold vs NHT), whereas NHT increased the size of knee flexors that do not extend the hip (1.9-fold vs LSET; both P ≤ 0.001). Changes in maximum eccentric torque differed only between LSET and CON (+17% vs +4%; P = 0.009), with NHT (+11%) inbetween. CONCLUSIONS: These results suggest that LSET is superior to NHT in inducing overall hamstrings and BFlh hypertrophy, potentially contributing to better sprint performance improvements and protection against hamstring strain injuries than NHT.

Smaller Biceps Femoris Aponeurosis Size in Legs with a History of Hamstring Strain Injury.

Biceps femoris long head (BFLH) aponeurosis size was compared between legs with and without prior hamstring strain injury (HSI) using two approaches: within-group (injured vs. uninjured legs of previous unilateral HSI athletes) and between-group (previously injured legs of HSI athletes vs. legs of No Prior HSI athletes). MRI scans were performed on currently healthy, competitive male athletes with Prior HSI history (n=23;≥1 verified BFLH injury; including a sub-group with unilateral HSI history; most recent HSI 1.6±1.2 years ago) and pair-matched athletes with No Prior HSI history (n=23). Anonymized axial images were manually segmented to quantify BFLH aponeurosis and muscle size. Prior unilateral HSI athletes' BFLH aponeurosis maximum width, aponeurosis area, and aponeurosis:muscle area ratio were 14.0-19.6% smaller in previously injured vs. contralateral uninjured legs (paired t-test, 0.008≤P≤0.044). BFLH aponeurosis maximum width and area were also 9.4-16.5% smaller in previously injured legs (n=28) from Prior HSI athletes vs. legs (n=46) of No Prior HSI athletes (unpaired t-test, 0.001≤P≤0.044). BFLH aponeurosis size was smaller in legs with prior HSI vs. those without prior HSI. These findings suggest BFLH aponeurosis size, especially maximum width, could be a potential cause or consequence of HSI, with prospective evidence needed to support or refute these possibilities.

Durability of Running Economy: Differences between Quantification Methods and Performance Status in Male Runners.

INTRODUCTION: Running economy (RE) deteriorates during prolonged running, although the effect of measuring energy cost (EC) or oxygen cost (OC) on the magnitude of these changes has not been investigated. Similarly, it is unknown if runners' performance level may influence the deterioration of RE during prolonged running. The aims of this study were to compare changes in EC and OC measurements of RE during a prolonged run in a large cohort of well-trained male runners, and to compare changes between runners of high and low performance standard. METHODS: Forty-four male runners (maximal oxygen uptake (V̇O2max) 62.4 ml·kg-1·min-1; 10 km time 35:50 ± 4:40 mm:ss) completed an incremental test determining lactate threshold 1 (LT1) and V̇O2max, and on a separate occasion, a 90 min run at LT1. Respiratory gases were collected at 15 min intervals. Subsequently, sub-groups of high- (HP, 10 km 31:20 ± 01:00 mm:ss) and low-performing (LP, 10 km 41:50 ± 01:20 mm:ss) runners were compared. RESULTS: RE deterioration was only fractionally larger when expressed as OC than EC (0.1% greater from 30-90 min; p < 0.001), perhaps due to the small change in RER (-0.01) in this study. For the HP group increases were lower than LP after 90 min in both EC (+2.3 vs +4.3%; p < 0.01) and OC (+2.4 vs +4.5%; p < 0.01). Similarly, at standardized distances, changes were lower for HP vs LP e.g. at 16.7 km +1.0 vs +3.2% for EC (p < 0.01), and + 1.2 vs +3.4% for OC (p < 0.001). CONCLUSIONS: The deterioration of RE was dependent on athlete's performance level, with HP runners displaying superior RE durability. The use of EC or OC had only a fractional influence on RE durability, although this may gain importance with larger shifts in substrate metabolism.

Long-Term Resistance Trained Human Muscles Have More Fibers, More Myofibrils, and Tighter Myofilament Packing Than Untrained.

INTRODUCTION: Increases in skeletal muscle size occur in response to prolonged exposure to resistance training that is typically ascribed to increased muscle fiber size. Whether muscle fiber number also changes remains controversial, and a paucity of data exists about myofibrillar structure. This cross-sectional study compared muscle fiber and myofibril characteristics in long-term resistance-trained (LRT) versus untrained (UNT) individuals. METHODS: The maximal anatomical cross-sectional area (ACSAmax) of the biceps brachii muscle was measured by magnetic resonance imaging in 16 LRT (5.9 ± 3.5 yr' experience) and 13 UNT males. A muscle biopsy was taken from the biceps brachii to measure muscle fiber area, myofibril area, and myosin spacing. Muscle fiber number, and myofibril number in total and per fiber were estimated by dividing ACSAmax by muscle fiber area or myofibril area, and muscle fiber area by myofibril area, respectively. RESULTS: Compared with UNT, LRT individuals had greater ACSAmax (+70%, P < 0.001), fiber area (+29%, P = 0.028), fiber number (+34%, P = 0.013), and myofibril number per fiber (+49%, P = 0.034) and in total (+105%, P < 0.001). LRT individuals also had smaller myosin spacing (-7%, P = 0.004; i.e., greater packing density) and a tendency toward smaller myofibril area (-16%, P = 0.074). ACSAmax was positively correlated with fiber area ( r = 0.526), fiber number ( r = 0.445), and myofibril number (in total r = 0.873 and per fiber r = 0.566), and negatively correlated with myofibril area ( r = -0.456) and myosin spacing ( r = -0.382) (all P < 0.05). CONCLUSIONS: The larger muscles of LRT individuals exhibited more fibers in cross-section and larger muscle fibers, which contained substantially more total myofibrils and more packed myofilaments than UNT participants, suggesting plasticity of muscle ultrastructure.

Comparison of Blood Flow Restriction Interventions to Standard Rehabilitation After an Anterior Cruciate Ligament Injury: A Systematic Review.

BACKGROUND: Blood flow restriction training (BFR-t) data are heterogeneous. It is unclear whether rehabilitation with BFR-t after an anterior cruciate ligament (ACL) injury is more effective in improving muscle strength and muscle size than standard rehabilitation. PURPOSE: To review outcomes after an ACL injury and subsequent reconstruction in studies comparing rehabilitation with and without BFR-t. STUDY DESIGN: Systematic review. Level of evidence, 3. METHODS: A search of English-language human clinical studies published in the past 20 years (2002-2022) was carried out in 5 health sciences databases, involving participants aged 18-65 undergoing rehabilitation for an ACL injury. Outcomes associated with muscle strength, muscle size, and knee-specific patient-reported outcome measures (PROMs) were extracted from studies meeting inclusion criteria and compared. RESULTS: The literature search identified 279 studies, of which 5 met the selection criteria. Two studies suggested that BFR-t rehabilitation after an ACL injury improved knee or thigh muscle strength and muscle size compared with rehabilitation consisting of comparable and higher load resistance training, with two studies suggesting the opposite. The single study measuring PROMs showed improvement compared to traditional rehabilitation, with no difference in muscle strength or size. CONCLUSION: BFR-t after an ACL injury seems to benefit muscle strength, muscle size, and PROM scores compared with standard rehabilitation alone. However, only 1 large study included all these outcomes, which has yet to be replicated in other settings. Further studies utilizing similar methods with a common set of outcome measures are required to confirm the effects of BFR-t on ACL rehabilitation.

Sex differences in muscle morphology between male and female sprinters.

There is a marked difference between males and females in sprint running performance, yet a comprehensive investigation of sex differences in the muscle morphology of sprinters, which could explain the performance differences, remains to be completed. This study compared muscle volumes of 23 individual leg muscles and 5 functional muscle groups, assessed with 3 T magnetic resonance imaging, between male (n = 31) and female (n = 22) sprinters, as well as subgroups of elite males (EM, n = 5), elite females (EF, n = 5), and performance-matched (to elite females) males (PMMEF, n = 6). Differences in muscle volume distribution between EM, EF, and unathletic male (UM) controls were also assessed. For the full cohorts, male sprinters were more muscular than their female counterparts, but the differences were nonuniform and anatomically variable, with the largest differences in the hip extensors and flexors. However, among elite sprinters the sex differences in the volume of the functional muscle groups were almost uniform (absolute volume +47-53%), and the muscle volume distribution of EM was more similar to EF than to UM (P < 0.039). For PMMEF, relative hip extensor volume, but not stature or percent body fat, differentiated for performance (PMMEF and EF < EM) rather than sex. In conclusion, although the full cohorts of sprinters showed a marked sex difference in the amount and distribution of muscle mass, elite sprinters appeared to be selected for a common muscle distribution phenotype that for these elite subgroups was a stronger effect than that of sex. Relative hip extensor muscle volume, rather than stature, percent body fat, or total relative muscle volume, appeared to be the primary determinant of the sex difference in performance.NEW & NOTEWORTHY We present novel evidence suggesting muscle volume, specifically relative hip extensor volume, may be a primary deterministic variable for the sex difference in sprint performance, such that with matched sprint times, male and female sprinters may be expected to have equivalent muscle morphology. We highlight striking similarities in distribution of leg muscle mass between elite male and female sprinters and provide evidence for the existence of a muscular distribution phenotype specific to elite sprinters, irrespective of sex.

Evidence for a new model of the complex interrelationship of ball possession, physical intensity and performance in elite soccer.

BACKGROUND: How the physical metrics, especially physical intensity, and possession interact with each other, and subsequently combine to influence performance remains opaque. Therefore, we investigated the interrelationship of possession, physical metrics, and team performance in elite soccer. METHODS: Four seasons of a top European league were used to derive 80 team league performances (points), together with possession and physical data. Physical metrics were absolute distances (m) during the whole match and ball-in-play, and rates of distance covered (m⋅min-1 ) as the index of physical intensity, notably when in-possession/out-of-possession, in total and within five speed categories. Interrelationships of possession, physical metrics, possession, and performance were assessed with Pearson's correlations and mediation analysis. RESULTS: Overall possession (r = 0.794) and time out-of-possession within the defensive third (r = -0.797) were most strongly correlated with performance. The strong relationships between in-possession distances and performance appeared coincidental due to greater time in-possession. Physical intensity had a complex relationship with possession and performance, with opposite relationships according to possession status: lower physical intensity when in-possession and higher physical intensity when out-of-possession were associated with possession and performance. Mediation analysis revealed the direct, independent importance of possession for team performance; however, the association of physical intensity with performance was largely (>79%) mediated by possession. CONCLUSION: Based on these findings, we propose a novel model of the interrelationships between possession, physical intensity, and performance, whereby higher possession is the largest, direct contributor toward enhanced team performance, with lower physical intensity in-possession a consequence of higher possession, but greater physical intensity when out-of-possession a cause of increased possession.

The Effect of Specific Bioactive Collagen Peptides on Tendon Remodeling during 15 wk of Lower Body Resistance Training.

PURPOSE: Collagen peptide supplementation has been reported to enhance synthesis rates or growth in a range of musculoskeletal tissues and could enhance tendinous tissue adaptations to resistance training (RT). This double-blind placebo-controlled study aimed to determine if tendinous tissue adaptations, size (patellar tendon cross-sectional area (CSA) and vastus lateralis (VL) aponeurosis area), and mechanical properties (patellar tendon), after 15 wk of RT, could be augmented with collagen peptide (CP) versus placebo (PLA) supplementation. METHODS: Young healthy recreationally active men were randomized to consume either 15 g of CP ( n = 19) or PLA ( n = 20) once every day during a standardized program of lower-body RT (3 times a week). Measurements pre- and post-RT included patellar tendon CSA and VL aponeurosis area (via magnetic resonance imaging), and patellar tendon mechanical properties during isometric knee extension ramp contractions. RESULTS: No between-group differences were detected for any of the tendinous tissue adaptations to RT (ANOVA group-time, 0.365 ≤ P ≤ 0.877). There were within-group increases in VL aponeurosis area (CP, +10.0%; PLA, +9.4%), patellar tendon stiffness (CP, +17.3%; PLA, +20.9%) and Young's modulus (CP, +17.8%; PLA, +20.6%) in both groups (paired t -tests (all), P ≤ 0.007). There were also within-group decreases in patellar tendon elongation (CP, -10.8%; PLA, -9.6%) and strain (CP, -10.6%; PLA, -8.9%) in both groups (paired t -tests (all), P ≤ 0.006). Although no within-group changes in patellar tendon CSA (mean or regional) occurred for CP or PLA, a modest overall time effect ( n = 39) was observed for mean (+1.4%) and proximal region (+2.4%) patellar tendon CSA (ANOVA, 0.017 ≤ P ≤ 0.048). CONCLUSIONS: In conclusion, CP supplementation did not enhance RT-induced tendinous tissue remodeling (either size or mechanical properties) compared with PLA within a population of healthy young men.

The effect of a prior eccentric lowering phase on concentric neuromechanics during multiple joint resistance exercise in older adults.

Aging involves a marked decline in physical function and especially muscle power. Thus, optimal resistance exercise (RE) to improve muscle power is required for exercise prescription. An eccentric lowering phase immediately before a concentric lift (ECC-CON) may augment concentric power production, due to various proposed mechanisms (e.g., elastic recoil, pre-activation, stretch reflex, contractile history), when compared with a concentric contraction alone (CON-Only). This study compared the effect of a prior eccentric lowering phase on older adult concentric power performance (ECC-CON vs. CON-Only) during a common multiple joint isoinertial RE (i.e., leg press) with a range of loads. Twelve healthy older adult males completed two measurement sessions, consisting of ECC-CON and CON-Only contractions, performed in a counterbalanced order using 20-80% of one repetition maximum [% 1RM] loads on an instrumented isoinertial leg press dynamometer that measured power, force, and velocity. Muscle activation was assessed with surface electromyography (sEMG). For mean power ECC-CON>CON-Only, with a pronounced effect of load on the augmentation of power by ECC-CON (+19 to +55%, 35-80% 1RM, all p < 0.032). Similarly, for mean velocity ECC-CON>CON-Only, especially as load increased (+15 to 54%, 20-80% 1RM, all p < 0.005), but mean force showed more modest benefits of ECC-CON (+9 to 14%, 50-80% 1RM, all p < 0.05). In contrast, peak power and velocity were similar for ECC-CON and CON-Only with all loads. Knee and hip extensor sEMG were similar for both types of contractions. In conclusion, ECC-CON contractions produced greater power, and velocity performance in older adults than CON-Only and may provide a superior stimulus for chronic power development.

The influence of ball in/out of play and possession in elite soccer: Towards a more valid measure of physical intensity during competitive match-play.

The physical demands of soccer match-play have typically been assessed using a low-resolution whole match approach ignoring whether the ball is in or out of play (BIP/BOP) and during these periods which team has possession. This study investigated the effect of fundamental match structure variables (BIP/BOP, in/out of possession) on the physical demands, and especially intensity, of elite match-play. For 1083 matches from a major European league, whole match duration, and player physical tracking data, were divided into BIP/BOP, and in/out of possession periods throughout the match, using on-ball event data. These distinct phases were used to derive absolute (m) and rate (m·min-1) of distance covered in total and within six speed categories during BIP/BOP and in/out possession. The rate of distance covered, an index of physical intensity, was >2-fold greater during BIP vs BOP. Whole match total distance covered was confounded by BIP time and poorly associated with physical intensity during BIP (r = 0.36). Whole match rates of distance covered substantially underestimated those during BIP, particularly for higher running speeds (∼-62%). Ball possession markedly effected physical intensity, with the rates of distance covered running (+31%), at high-speed (+30%) and in total (+7%) greater out than in possession. Whole match physical metrics underestimated the physical intensity during BIP, and thus the rate(s) of distance covered during BIP are recommended for accurate measurement of physical intensity in elite soccer. The greater demands of being out of possession support a possession-based tactical approach to minimise fatigue and its negative consequences.

This large-scale study utilising >1000 elite level competitive matches found profound differences in rate of distance covered between periods of BIP vs BOP, being 2-fold higher overall and 8- to 33-fold higher for the rates of distance covered within running, high-speed and sprinting speed categories.Consequently, commonly used whole match physical metrics, that incorporate both BIP and BOP, such as distances covered but even rates of distance covered, were not valid indices of physical intensity (rate of distance covered) during BIP.Thus a more valid and direct approach to quantifying physical intensity during elite soccer match-play as the rate of distance covered during BIP is proposed.Utilising a unique within-match analysis the effect of possession (i.e. in vs out) revealed that teams covered ≥30% more running and high-speed distance while out than in possession during BIP.

Non-invasive estimation of muscle fibre size from high-density electromyography.

Because of the biophysical relation between muscle fibre diameter and the propagation velocity of action potentials along the muscle fibres, motor unit conduction velocity could be a non-invasive index of muscle fibre size in humans. However, the relation between motor unit conduction velocity and fibre size has been only assessed indirectly in animal models and in human patients with invasive intramuscular EMG recordings, or it has been mathematically derived from computer simulations. By combining advanced non-invasive techniques to record motor unit activity in vivo, i.e. high-density surface EMG, with the gold standard technique for muscle tissue sampling, i.e. muscle biopsy, here we investigated the relation between the conduction velocity of populations of motor units identified from the biceps brachii muscle, and muscle fibre diameter. We demonstrate the possibility of predicting muscle fibre diameter (R2  = 0.66) and cross-sectional area (R2  = 0.65) from conduction velocity estimates with low systematic bias (∼2% and ∼4% respectively) and a relatively low margin of individual error (∼8% and ∼16%, respectively). The proposed neuromuscular interface opens new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling. The non-invasive nature of high-density surface EMG for the assessment of muscle fibre size may be useful in studies monitoring child development, ageing, space and exercise physiology, although the applicability and validity of the proposed methodology need to be more directly assessed in these specific populations by future studies. KEY POINTS: Because of the biophysical relation between muscle fibre size and the propagation velocity of action potentials along the sarcolemma, motor unit conduction velocity could represent a potential non-invasive candidate for estimating muscle fibre size in vivo. This relation has been previously assessed in animal models and humans with invasive techniques, or it has been mathematically derived from simulations. By combining high-density surface EMG with muscle biopsy, here we explored the relation between the conduction velocity of populations of motor units and muscle fibre size in healthy individuals. Our results confirmed that motor unit conduction velocity can be considered as a novel biomarker of fibre size, which can be adopted to predict muscle fibre diameter and cross-sectional area with low systematic bias and margin of individual error. The proposed neuromuscular interface opens new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling.

Motor Unit Discharge Characteristics and Conduction Velocity of the Vastii Muscles in Long-Term Resistance-Trained Men.

PURPOSE: Adjustments in motor unit (MU) discharge properties have been shown after short-term resistance training; however, MU adaptations in long-term resistance-trained (RT) individuals are less clear. Here, we concurrently assessed MU discharge characteristics and MU conduction velocity in long-term RT and untrained (UT) men. METHODS: Motor unit discharge characteristics (discharge rate, recruitment, and derecruitment threshold) and MU conduction velocity were assessed after the decomposition of high-density electromyograms recorded from vastus lateralis (VL) and vastus medialis (VM) of RT (>3 yr; n = 14) and UT ( n = 13) during submaximal and maximal isometric knee extension. RESULTS: Resistance-trained men were on average 42% stronger (maximal voluntary force [MVF], 976.7 ± 85.4 N vs 685.5 ± 123.1 N; P < 0.0001), but exhibited similar relative MU recruitment (VL, 21.3% ± 4.3% vs 21.0% ± 2.3% MVF; VM, 24.5% ± 4.2% vs 22.7% ± 5.3% MVF) and derecruitment thresholds (VL, 20.3% ± 4.3% vs 19.8% ± 2.9% MVF; VM, 24.2% ± 4.8% vs 22.9% ± 3.7% MVF; P ≥ 0.4543). There were also no differences between groups in MU discharge rate at recruitment and derecruitment or at the plateau phase of submaximal contractions (VL, 10.6 ± 1.2 pps vs 10.3 ± 1.5 pps; VM, 10.7 ± 1.6 pps vs 10.8 ± 1.7 pps; P ≥ 0.3028). During maximal contractions of a subsample population (10 RT, 9 UT), MU discharge rate was also similar in RT compared with UT (VL, 21.1 ± 4.1 pps vs 14.0 ± 4.5 pps; VM, 19.5 ± 5.0 pps vs 17.0 ± 6.3 pps; P = 0.7173). Motor unit conduction velocity was greater in RT compared with UT individuals in both VL (4.9 ± 0.5 m·s -1 vs 4.5 ± 0.3 m·s -1 ; P < 0.0013) and VM (4.8 ± 0.5 m·s -1 vs 4.4 ± 0.3 m·s -1 ; P < 0.0073). CONCLUSIONS: Resistance-trained and UT men display similar MU discharge characteristics in the knee extensor muscles during maximal and submaximal contractions. The between-group strength difference is likely explained by superior muscle morphology of RT as suggested by greater MU conduction velocity.

The effect of specific bioactive collagen peptides on function and muscle remodeling during human resistance training.

AIM: Bioactive collagen peptides (CP) have been suggested to augment the functional, structural (size and architecture), and contractile adaptations of skeletal muscle to resistance training (RT), but with limited evidence. This study aimed to determine if CP vs. placebo (PLA) supplementation enhanced the functional and underpinning structural, and contractile adaptations after 15 weeks of lower body RT. METHODS: Young healthy males were randomized to consume either 15 g of CP (n = 19) or PLA (n = 20) once every day during a standardized program of progressive knee extensor, knee flexor, and hip extensor RT 3 times/wk. Measurements pre- and post-RT included: knee extensor and flexor isometric strength; quadriceps, hamstrings, and gluteus maximus volume with MRI; evoked twitch contractions, 1RM lifting strength, and architecture (with ultrasound) of the quadriceps. RESULTS: Percentage changes in maximum strength (isometric or 1RM) did not differ between-groups (0.684 ≤ p ≤ 0.929). Increases in muscle volume were greater (quadriceps 15.2% vs. 10.3%; vastus medialis (VM) 15.6% vs. 9.7%; total muscle volume 15.7% vs. 11.4%; [all] p ≤ 0.032) or tended to be greater (hamstring 16.5% vs. 12.8%; gluteus maximus 16.6% vs. 12.9%; 0.089 ≤ p ≤ 0.091) for CP vs. PLA. There were also greater increases in twitch peak torque (22.3% vs. 12.3%; p = 0.038) and angle of pennation of the VM (16.8% vs. 5.8%, p = 0.046), but not other muscles, for CP vs. PLA. CONCLUSIONS: CP supplementation produced a cluster of consistent effects indicating greater skeletal muscle remodeling with RT compared to PLA. Notably, CP supplementation amplified the quadriceps and total muscle volume increases induced by RT.

The Muscle Morphology of Elite Female Sprint Running.

INTRODUCTION: A paucity of research exists examining the importance of muscle morphological and functional characteristics for elite female sprint performance. PURPOSE: This study aimed to compare lower body muscle volumes and vertical jumping power between elite and subelite female sprinters and assess the relationships of these characteristics with sprint race and acceleration performance. METHODS: Five elite (100 m seasons best [SBE 100 ], 11.16 ± 0.06 s) and 17 subelite (SBE 100 , 11.84 ± 0.42 s) female sprinters underwent: 3T magnetic resonance imaging to determine the volume of 23 individual leg muscles/compartments and five functional muscle groups; countermovement jump and 30 m acceleration tests. RESULTS: Total absolute lower body muscle volume was higher in elite versus subelite sprinters (+15%). Elite females exhibited greater muscle volume of the hip flexors (absolute, +28%; relative [to body mass], +19%), hip extensors (absolute, +22%; relative, +14%), and knee extensors (absolute, +21%), demonstrating pronounced anatomically specific muscularity, with relative hip flexor volume alone explaining 48% of sprint performance variability. The relative volume of five individual muscles (sartorius, gluteus maximus, adductor magnus, vastus lateralis, illiopsoas) were both distinct between groups (elite > subelite) and related to SBE 100 ( r = 0.553-0.639), with the combination of the sartorius (41%) and the adductor magnus (17%) explaining 58% of the variance in SBE 100 . Elite female sprinters demonstrated greater absolute countermovement jump power versus subelite, and absolute and relative power were related to both SBE 100 ( r = -0.520 to -0.741) and acceleration performance ( r = 0.569 to 0.808). CONCLUSIONS: This investigation illustrates the distinctive, anatomically specific muscle volume distribution that facilitates elite sprint running in females, and emphasizes the importance of hip flexor and extensor relative muscle volume.

Startling stimuli increase maximal motor unit discharge rate and rate of force development in humans.

Maximal rate of force development in adult humans is determined by the maximal motor unit discharge rate, however, the origin of the underlying synaptic inputs remains unclear. Here, we tested a hypothesis that the maximal motor unit discharge rate will increase in response to a startling cue, a stimulus that purportedly activates the pontomedullary reticular formation neurons that make mono- and disynaptic connections to motoneurons via fast-conducting axons. Twenty-two men were required to produce isometric knee extensor forces "as fast and as hard" as possible from rest to 75% of maximal voluntary force, in response to visual (VC), visual-auditory (VAC; 80 dB), or visual-startling cue (VSC; 110 dB). Motoneuron activity was estimated via decomposition of high-density surface electromyogram recordings over the vastus lateralis and medialis muscles. Reaction time was significantly shorter in response to VSC compared with VAC and VC. The VSC further elicited faster neuromechanical responses including a greater number of discharges per motor unit per second and greater maximal rate of force development, with no differences between VAC and VC. We provide evidence, for the first time, that the synaptic input to motoneurons increases in response to a startling cue, suggesting a contribution of subcortical pathways to maximal motoneuron output in humans.NEW & NOTEWORTHY Motor unit discharge characteristics are a key determinant of rate of force development in humans, but the neural substrate(s) underpinning such output remains unknown. Using decomposition of high-density electromyogram, we show greater number of discharges per motor unit per second and greater rate of force development after a startling auditory stimulus. These observations suggest a possible subcortical contribution to maximal in vivo motor unit discharge rate in adult humans.

Neural decoding from surface high-density EMG signals: influence of anatomy and synchronization on the number of identified motor units.

Objective.High-density surface electromyography (HD-sEMG) allows the reliable identification of individual motor unit (MU) action potentials. Despite the accuracy in decomposition, there is a large variability in the number of identified MUs across individuals and exerted forces. Here we present a systematic investigation of the anatomical and neural factors that determine this variability.Approach. We investigated factors of influence on HD-sEMG decomposition, such as synchronization of MU discharges, distribution of MU territories, muscle-electrode distance (MED-subcutaneous adipose tissue thickness), maximum anatomical cross-sectional area (ACSAmax), and fiber cross-sectional area. For this purpose, we recorded HD-sEMG signals, ultrasound and magnetic resonance images, and took a muscle biopsy from the biceps brachii muscle from 30 male participants drawn from two groups to ensure variability within the factors-untrained-controls (UT = 14) and strength-trained individuals (ST = 16). Participants performed isometric ramp contractions with elbow flexors (at 15%, 35%, 50% and 70% maximum voluntary torque-MVT). We assessed the correlation between the number of accurately detected MUs by HD-sEMG decomposition and each measured parameter, for each target force level. Multiple regression analysis was then applied.Main results.ST subjects showed lower MED (UT = 5.1 ± 1.4 mm; ST = 3.8 ± 0.8 mm) and a greater number of identified MUs (UT: 21.3 ± 10.2 vs ST: 29.2 ± 11.8 MUs/subject across all force levels). The entire cohort showed a negative correlation between MED and the number of identified MUs at low forces (r= -0.6,p= 0.002 at 15% MVT). Moreover, the number of identified MUs was positively correlated to the distribution of MU territories (r= 0.56,p= 0.01) and ACSAmax(r= 0.48,p= 0.03) at 15% MVT. By accounting for all anatomical parameters, we were able to partly predict the number of decomposed MUs at low but not at high forces.Significance.Our results confirmed the influence of subcutaneous tissue on the quality of HD-sEMG signals and demonstrated that MU spatial distribution and ACSAmaxare also relevant parameters of influence for current decomposition algorithms.

Fast and ballistic contractions involve greater neuromuscular power production in older adults during resistance exercise.

PURPOSE: Neuromuscular power is critical for healthy ageing. Conventional older adult resistance training (RT) guidelines typically recommend lifting slowly (2-s; CONV), whereas fast/explosive contractions performed either non-ballistically (FAST-NB) or ballistically (FAST-B, attempting to throw the load) may involve greater acute power production, and could ultimately provide a greater chronic power adaptation stimulus. To compare the neuromechanics (power, force, velocity, and muscle activation) of different types of concentric isoinertial RT contractions in older adults. METHODS: Twelve active older adult males completed three sessions, each randomly assigned to one type of concentric contraction (CONV or FAST-NB or FAST-B). Each session involved lifting a range of loads (20-80%1RM) using an instrumented isoinertial leg press dynamometer that measured power, force, and velocity. Muscle activation was assessed with surface electromyography (sEMG). RESULTS: Peak and mean power were markedly different, according to the concentric contraction explosive intent FAST-B > FAST-NB > CONV, with FAST-B producing substantially more power (+ 49 to 1172%, P ≤ 0.023), force (+ 10 to 136%, P < 0.05) and velocity (+ 55 to 483%, P ≤ 0.025) than CONV and FAST-NB contractions. Knee and hip extensor sEMG were typically higher during FAST-B than CON (all P < 0.02) and FAST-NB (≤ 50%1RM, P ≤ 0.001). CONCLUSIONS: FAST-B contractions produced markedly greater power, force, velocity and muscle activation across a range of loads than both CONV or FAST-NB and could provide a more potent RT stimulus for the chronic development of older adult power.