Cannabidiol induces systemic analgesia through activation of the PI3Kγ/nNOS/NO/KATP signaling pathway in neuropathic mice. A KATP channel S-nitrosylation-dependent mechanism.

BACKGROUND: Cannabidiol (CBD) is the second most abundant pharmacologically active component present in Cannabis sp. Unlike Δ-9-tetrahydrocannabinol (THC), it has no psychotomimetic effects and has recently received significant interest from the scientific community due to its potential to treat anxiety and epilepsy. CBD has excellent anti-inflammatory potential and can be used to treat some types of inflammatory and neuropathic pain. In this context, the present study aimed to evaluate the analgesic mechanism of cannabidiol administered systemically for the treatment of neuropathic pain and determine the endogenous mechanisms involved with this analgesia. METHODS: Neuropathic pain was induced by sciatic nerve constriction surgery, and the nociceptive threshold was measured using the paw compression test in mice. RESULTS: CBD produced dose-dependent antinociception after intraperitoneal injection. Selective inhibition of PI3Kγ dose-dependently reversed CBD-induced antinociception. Selective inhibition of nNOS enzymes reversed the antinociception induced by CBD, while selective inhibition of iNOS and eNOS did not alter this antinociception. However, the inhibition of cGMP production by guanylyl cyclase did not alter CBD-mediated antinociception, but selective blockade of ATP-sensitive K+ channels dose-dependently reversed CBD-induced antinociception. Inhibition of S-nitrosylation dose-dependently and completely reversed CBD-mediated antinociception. CONCLUSION: Cannabidiol has an antinociceptive effect when administered systemically and this effect is mediated by the activation of PI3Kγ as well as by nitric oxide and subsequent direct S-nitrosylation of KATP channels on peripheral nociceptors.

The care of the patients with hipoplastic left heart syndrome in places of social and economic vulneability. An ethical analysis.

The birth of a child means hope and joy, particularly for the parents and the healthcare team. When this child is born with a severe malformation and a poor prognosis, as in the case of hypoplastic left heart syndrome, the scenario is one of great uncertainty and emotional suffering. The role of the health team becomes fundamental for the identification of conflicts of values and for the search for shared decisions that promote the best benefit to the child. When the diagnosis is made during fetal life, it is necessary to develop counseling strategies appropriate to the context of each family. In places with limited care resources, precarious prenatal care and short temporal conditions, the recommended counseling is compromised. Indication of treatment requires technical competence and a detailed analysis of ethical issues, and consultation with institutional clinical bioethics services or commissions is important. The article proposes to address the moral conflicts of two clinical cases and the respective bioethical analysis that involves principles and values in contexts of vulnerability and uncertainty, contrasting two situations where the indication of treatment was based on accessibility to treatment.

Peripherally injected canabidiol reduces neuropathic pain in mice: Role of the 5-HT1A and TRPV1 receptors.

Cannabidiol (CBD) is the most abundant non-psychoactive component found in plants of the genus Cannabis. Its analgesic effect for the treatment of neuropathy has been widely studied. However, little is known about its effects in the acute treatment when Cannabidiol is administered peripherally. Because of that, this research was aimed to evaluate the antinociceptive effects of the CBD when administered peripherally for the treatment of acute neuropathic pain and check the involvement of the 5-HT1A and the TRPV1 receptors in this event. Neuropathic pain was induced with the constriction of the sciatic nerve while the nociceptive threshold was measured using the pressure test of the mouse paw. The technique used proved to be efficient to induce neuropathy, and the CBD (5, 10 and 30 µg/paw) induced the antinociception in a dosage-dependent manner. The dosage used that induced a more potent effect (30 µg/paw), did not induce a systemic response, as demonstrated by both the motor coordination assessment test (RotaRod) and the antinociceptive effect restricted to the paw treated with CBD. The administration of NAN-190 (10 µg/paw), a selective 5-HT1A receptor antagonist, and SB-366791 (16 µg/paw), a selective TRPV1 antagonist, partially reversed the CBD-induced antinociception. The results of the research suggest that the CBD produces the peripheral antinociception during the acute treatment of the neuropathic pain and it partially involved the participation of the 5-HT1A and TRPV1 receptors.

Detection of Toxoplasma gondii DNA in milk of dairy cows from southern Brazil.

Consumption of unpasteurized cow's milk may be a transmission route for some pathogenic microorganisms, but there is little information about the risk of Toxoplasma gondii infection. Blood and milk samples were collected in a paired and random fashion from 106 dairy cows and bulk-tank milk samples were also collected from each of the six farms, in southern Brazil. Serum anti-T.gondii antibodies (IgG) were detected by an indirect fluorescent antibody test (IFAT) with a cutoff point of 1:64. Nested PCR targeting the ITS1 was performed on milk samples to detect the Sarcocystidae family, confirmed to be T.gondii by Sanger sequencing. The occurrence of anti-T.gondii antibodies in the herds was 14.1%, (15/106) with seropositive cows in all herds. Antibody titers in positive samples ranged from 64 to 128. T.gondii DNA was detected in 2.8% (03/106) of the milk samples. The ITS1 sequences generated in this study were ON809793 - ON809794 and the sequencing revealed 98-100% identity with T. gondii DNA sequences deposited in GenBank. All cows PCR positive for T.gondii in milk were negative for IgG antibodies in serum, suggesting that naturally infected cows may shed T. gondii in milk in the acute phase of infection. The results of this study demonstrate that T. gondii DNA may be detected in raw cow's milk, so the potential risks of lactogenic infection should be considered. The presence of T. gondii DNA in milk does not confirm that the protozoa are viable and infective, and further investigations into the role of cow's milk in the epidemiology of toxoplasmosis are needed.

The care of the patients with hipoplastic left heart syndrome in places of social and economic vulneability. An ethical analysis / O cuidado de pacientes com síndrome do coração esquerdo hipoplásico em localidades de vulnerabilidade social e econômica. Uma análise bioética

ABSTRACT The birth of a child means hope and joy, particularly for the parents and the healthcare team. When this child is born with a severe malformation and a poor prognosis, as in the case of hypoplastic left heart syndrome, the scenario is one of great uncertainty and emotional suffering. The role of the health team becomes fundamental for the identification of conflicts of values and for the search for shared decisions that promote the best benefit to the child. When the diagnosis is made during fetal life, it is necessary to develop counseling strategies appropriate to the context of each family. In places with limited care resources, precarious prenatal care and short temporal conditions, the recommended counseling is compromised. Indication of treatment requires technical competence and a detailed analysis of ethical issues, and consultation with institutional clinical bioethics services or commissions is important. The article proposes to address the moral conflicts of two clinical cases and the respective bioethical analysis that involves principles and values in contexts of vulnerability and uncertainty, contrasting two situations where the indication of treatment was based on accessibility to treatment.

RESUMO O nascimento de uma criança significa esperança e alegria, particularmente para os pais e para a equipe de saúde. Quando essa criança nasce com uma malformação grave e de prognóstico reservado, como acontece na SHCE, o cenário é de grande incerteza e sofrimento emocional. O papel da equipe de saúde torna-se fundamental para a identificação de conflitos de valores e para a busca de decisões compartilhadas que promovam o melhor benefício à criança. Quando o diagnóstico é feito em vida fetal, é necessário a elaboração de estratégias de aconselhamento adequadas ao contexto de cada família. Em locais com recursos assistenciais limitados, acompanhamento prénatal precário e condição temporal curta, o aconselhamento preconizado fica comprometido. A indicação do tratamento requer competência técnica e uma análise pormenorizada de questões éticas, sendo importante a consultoria aos serviços ou comissões de bioética clínica institucional. O artigo propõe a abordar os conflitos morais de dois casos clínicos e a respectiva análise bioética que envolve princípios e valores em contextos de vulnerabilidade e incertezas, contrapondo duas situações onde a indicação do tratamento baseou-se na acessibilidade ao tratamento.

The impact of intrinsic capacity and physical resilience on health outcomes: a case report / O impacto da capacidade intrínseca e da resiliência física em desfechos de saúde: um relato de caso

Justification and objective: The physiological changes that accompany human aging increase the risk of disease and care dependence. To meet the health needs of older adults, care must go beyond diagnosing specific diseases and assess the impact of these conditions on functional capacity. The concepts of intrinsic capacity and physical resilience aim to explain the trajectory of various health outcomes in older adults. The case presented below illustrates the positive impact of intrinsic capacity, together with external resources, on the prognosis of an older patient during a catastrophic health event. Case description: A 91-year-old widower was diagnosed with severe dehydration, delirium, pneumonia, and a grade IV pressure injury after lying in his home for 6 days. He remained hospitalized for 87 days and was discharged with almost complete recovery of his functional capacity. Discussion: The main point of this case is the positive health outcome experienced by a nonagenarian patient given the severity of the events. From the perspective of intrinsic capacity, he was functionally independent, had good sensory function, excellent cognition and vitality, and his psychological status was positive. He is currently progressing towards complete functional recovery. (AU)

Justificativa e objetivo: As alterações fisiológicas que acompanham o envelhecimento humano aumentam o risco do desenvolvimento de doenças e dependência de cuidados. Para avaliar as necessidades de saúde de um indivíduo idoso, mais do que diagnosticar doenças específicas, o mais importante é analisar o impacto dessas condições em sua capacidade funcional. Os conceitos de capacidade intrínseca (CI) e resiliência física têm a finalidade de explicar a trajetória de diversos desfechos de saúde em idosos. O caso apresentado a seguir visa ilustrar o impacto positivo da CI, em conjunto com os recursos externos, no prognóstico de um paciente idoso durante evento catastrófico de saúde. Descrição do caso: Masculino, 91 anos, viúvo, diagnosticado com desidratação grave, delirium, pneumonia e lesão por pressão grau IV ao fim de seis dias caído em sua casa. Permaneceu hospitalizado por 87 dias e obteve alta com recuperação quase completa de sua capacidade funcional. Discussão: O ponto central do caso é o desfecho positivo de saúde vivido por um paciente nonagenário diante da gravidade dos eventos. Do ponto de vista da CI, ele era independente funcional, tinha boa função sensorial, excelente cognição e vitalidade, e seu estado psicológico era positivo. Atualmente, evolui com completa recuperação funcional. (AU)

Bradykinin induces peripheral antinociception in PGE2-induced hyperalgesia in mice.

BACKGROUND: Bradykinin (BK) is an endogenous peptide involved in vascular permeability and inflammation. It has opposite effects (inducing hyperalgesia or antinociception) when administered directly in the central nervous system. The aim of this study was to evaluate whether BK may also present this dual effect when injected peripherally in a PGE2-induced nociceptive pain model, as well as to investigate the possible mechanisms of action involved in this event in mice. METHODS: Male Swiss and C57BL/6 knockout mice for B1 or B2 bradykinin receptors were submitted to a mechanical paw pressure test and hyperalgesia was induced by intraplantar prostaglandin E2 (2 µg/paw) injection. RESULTS: Bradykinin (20, 40 and 80 ng/paw) produced dose-dependent peripheral antinociception against PGE2-induced hyperalgesia. This effect was antagonized by bradyzide (8, 16 and 32 µg/paw), naloxone (12.5, 25 and 50 µg/paw), nor-binaltorphimine (50, 100 and 200 µg/paw) and AM251 (20, 40 and 80 µg/paw). Bestatin (400 µg/paw), MAFP (0.5 µg/paw) and VDM11 (2.5 µg/paw) potentiated the antinociception of a lower 20 ng BK dose. The knockout of B1 or B2 bradykinin receptors partially abolished the antinociceptive action of BK (80 ng/paw), bremazocine (1 µg/paw) and anandamide (40 ng/paw) when compared with wild-type animals, which show complete antinociception with the same dose of each drug. CONCLUSION: The present study is the first to demonstrate BK-induced antinociception in peripheral tissues against PGE2-induced nociception in mice and the involvement of κ-opioid and CB1 cannabinoid receptors in this effect.

Analysis of potential drug interactions in medical clinic sector in a Hospital of João Pessoa - PB

Abstract The objective of this study was to evaluate drug interactions based on medical records of patients hospitalized in University Hospital Lauro Wanderley (UHLW) in João Pessoa-PB, Brazil. This was a quantitative, descriptive study with a cross-sectional design. This research was conducted in the medical clinic of the above hospital by analyzing pharmaceutical intervention in medical records. The investigated samples consisted of all medical profiles with drug interaction information of patients hospitalized from June 2016 to June 2017. Most of these drug interactions were determined and classified by Micromedex® Solutions database. This research was approved by the Ethics Committee in Institutional Human Research, protocol number 2.460.206. In total, 331 drug interactions were found in 131 medical profiles. Dipyrone, enoxaparin, sertraline, ondansetron, quetiapine, tramadol, bromopride, amitriptyline, and simvastatin were medications that showed highest interactions. According to Anatomical Therapy Classification (ATC), drugs that act on the central nervous system result in more interactions. The most prevalent interaction was between dipyrone and enoxaparin. Some limitations of this study are the lack of notifications and data on drug interactions.

Transplacental transmission of Neospora caninum to lambs in successive pregnancies of naturally infected sheep in Southern Brazil.

This study aims to report the detection of N. caninum DNA in a newborn lamb (1) with neurological signs and congenital neosporosis and in a stillborn lamb (2), both born from the same ewe in a herd of Southern Brazil. The lambs were born during different pregnancies of a Suffolk ewe seropositive to N. caninum and seronegative to T. gondii. Histopathological lesions were observed only in the central nervous system of the lambs. The newborn lamb (1) showed mild and focal gliosis in the frontal lobe. In the hippocampal region of the stillborn lamb (2), lymphoplasmacytic perivascular cuffs and N. caninum cysts were observed in the cytoplasm of neurons and confirmed by IHC. PCR was performed using brain samples to detect the protozoa N. caninum and Toxoplasma gondii. The infection with N. caninum was confirmed in the newborn lamb (1) by PCR and in the stillborn lamb (2) by histopathology, immunohistochemistry, and PCR tests.

Serosurvey of Toxoplasma gondii, Neospora caninum and Sarcocystis neurona in raptors and risk factor analysis.

Raptors are carnivorous birds with great hunting ability. Toxoplasma gondii, Neospora caninum and Sarcocystis spp. are intracellular Apicomplexan protozoans which infect a wide range of intermediate hosts, including birds. The aims of this study were to evaluate the serological reactivity of captive raptors serum to T. gondii, N. caninum and S. neurona antigens and identify possible risk factors associated with the infection. From August 2014 to September 2015, blood samples from 72 raptors were collected and serum samples were tested by immunofluorescence antibody test (IFAT). Antigen slides were prepared using tachyzoites of T. gondii and N. caninum and using merozoites of S. neurona. Serum samples were tested at the following cut-off dilutions: 1:16 for T. gondii and 1:50 for N. caninum and S. neurona. An anti-chicken IgY antibody conjugated with FITC was used as a secondary antibody at 1:50 dilution. Out of the 72 raptors serum tested by IFAT, 2.7% reacted to N. caninum, 8.3% to T. gondii and 11.1% to S. neurona antigens. The region in which the sample was collected, the reason the raptors were kept in captivity and diet were statistically associated with seropositivity to T. gondii and the use of the birds and diet were statistically associated with seropositivity to N. caninum and S. neurona (p ≤ 0.05). We highlight the occurrence of these protozoans in birds of prey and the importance of good hygiene and feeding management of these birds in captivity to reduce the risk of protozoal infections.

Small cyclic sodium channel inhibitors.

Voltage-gated sodium (NaV) channels play crucial roles in a range of (patho)physiological processes. Much interest has arisen within the pharmaceutical industry to pursue these channels as analgesic targets following overwhelming evidence that NaV channel subtypes NaV1.7-NaV1.9 are involved in nociception. More recently, NaV1.1, NaV1.3 and NaV1.6 have also been identified to be involved in pain pathways. Venom-derived disulfide-rich peptide toxins, isolated from spiders and cone snails, have been used extensively as probes to investigate these channels and have attracted much interest as drug leads. However, few peptide-based leads have made it as drugs due to unfavourable physiochemical attributes including poor in vivo pharmacokinetics and limited oral bioavailability. The present work aims to bridge the gap in the development pipeline between drug leads and drug candidates by downsizing these larger venom-derived NaV inhibitors into smaller, more "drug-like" molecules. Here, we use molecular engineering of small cyclic peptides to aid in the determination of what drives subtype selectivity and molecular interactions of these downsized inhibitors across NaV subtypes. We designed a series of small, stable and novel NaV probes displaying NaV subtype selectivity and potency in vitro coupled with potent in vivo analgesic activity, involving yet to be elucidated analgesic pathways in addition to NaV subtype modulation.

Purified glycerin in balanced diets for chicken broilers from 8 to 21 days old / Glicerina purificada em dietas balanceadas para frangos de corte dos 8 aos 21 dias de idade

This paper aimed to evaluate the metabolizability, performance and economic viability of purified glycerin inclusion in balanced diets fed to chicken broilers from 8 to 21 days old. Two experiments were conducted. In the first experiment, 100 broilers (14 days old) were distributed in a completely randomized design into two treatments, with five replications of 10 broilers. Treatments consisted of a control diet and a test diet, in which purified glycerin replaced 10% of the control diet. In the second experiment, 200 broilers (8 days old) were distributed in a completely randomized design into four treatments (0, 2, 4 and 6% of purified glycerin inclusion), with five replications of 10 broilers. The weight gain, feed intake, feed conversion, final weight, apparent metabolizable energy (AME), nitrogen-corrected apparent metabolizable energy (AMEn), metabolizability coefficients of dry matter (DMMC), crude protein (CPMC) and gross energy (GEMC), and the cost of feed per kg of broiler produced were evaluated. The AME, AMEn, DMMC, CPMC and GEMC from the purified glycerin were 3790 and 3560 kcal/kg, and 83.72, 71.52 and 86.27%, respectively. The glycerin levels did not affect (p>0.05) any of the performance characteristics (weight gain, feed intake, feed conversion and final weight). The lowest feeding cost and the highest gross margin were obtained for broilers fed with 6% purified glycerin. The inclusion of 6% purified glycerin in balanced diets for broilers from 8 to 21 days old was technically and economically feasible.

Objetivou-se neste trabalho avaliar a metabolizabilidade, o desempenho zootécnico e a viabilidade econômica da inclusão de glicerina purificada, em dietas balanceadas para frangos de corte dos 8 aos 21 dias de idade. Foram realizados dois experimentos, sendo que, no primeiro experimento, foram utilizados 100 pintos de 14 dias de idade, distribuídos em delineamento inteiramente casualizado (DIC), com dois tratamentos, cinco repetições de 10 aves. Os tratamentos consistiram de uma dieta referência e uma dieta teste, na qual a glicerina purificada substituiu 10% da dieta referência. No segundo experimento, foram utilizados 200 pintos de 8 dias de idade, distribuídos em delineamento experimental inteiramente casualizado (DIC), com quatro tratamentos (0, 2, 4 e 6% de inclusão de glicerina purificada) e cinco repetições de 10 aves. Foram determinados o ganho de peso, consumo de ração, conversão alimentar, peso final, energia metabolizável aparente (EMA), energia metabolizável aparente corrigida pelo balanço de nitrogênio (EMAn), os coeficientes de metabolizabilidade da matéria seca (CMMS), proteína bruta (CMPB), energia bruta (CMEB) e o custo da alimentação por kg de frango produzido. A EMA, EMAn e os CMMS, CMPB, CMEB da glicerina purificada obtida foram de 3790, 3560 Kcal/kg e 83,72, 71,52, 86,27%, respectivamente. Observou-se que a inclusão de glicerina purificada não afetou (p>0,05) o desempenho (ganho de peso, consumo de ração, conversão alimentar e peso final). O menor custo com a alimentação e a maior margem bruta foi obtido com os frangos alimentados com 6% de inclusão de glicerina purificada. A inclusão de 6% de glicerina purificada em dietas balanceadas para frangos de corte dos 8 aos 21 dias de idade, mostrou-se técnica e economicamente viável.

Purified glycerin diets for broilers from 8 to 42 days old / Glicerina purificada em dietas para frangos de corte dos 8 aos 42 dias de idade

Here we evaluate the technical viability of including purified glycerin in balanced diets for broilers from 8 to 42 days old. For this, we used 160 8-day-old broiler chickens from the Cobb 500® lineage, distributed in a completely randomized design (CRD) with four treatments (0, 2, 4, and 6% of purified glycerin inclusion) and four replicates of ten birds. We evaluated feed intake, weight gain, feed conversion, final weight, organs biometry (heart, liver, gizzard and small intestine), carcass yield, special cuts yields (thigh, drumstick, and breast), meat color, and protein and fat deposition in the breast muscle. The purified glycerin inclusion levels in diets influenced (p < 0.05) weight gain, feed conversion, and broiler weight at 42 days, with no effect (p > 0.05) on feed intake (FI). Similarly, there was no effect (p > 0.05) on carcass and special cuts yields. The purified glycerin inclusion levels did not influence (p > 0.05) the relative gizzard weight, nor the relative small intestine weight and length. However, the purified glycerin inclusion levels did affect the relative heart and liver weights (p < 0.05). The purified glycerine inclusion levels in diets did not affect (p > 0.05) lightness (L*), redness (a*), yellowness (b*) values, pH, or protein deposition, but did increases (p < 0.05) fat deposition in breast muscle. Based on our findings, we propose that the inclusion of 6% purified glycerin in diets is technically feasible for broilers of 8 to 42 days old.(AU)

Objetivou-se neste trabalho avaliar a viabilidade técnica da inclusão de glicerina purificada em dietas balanceadas para frangos de corte, dos 8 aos 42 dias de idade. Foram utilizados 160 pintos de corte, com 8 dias, da linhagem Cobb 500®, distribuídos em delineamento experimental inteiramente casualizado (DIC), com quatro tratamentos (0, 2, 4 e 6% de inclusão de glicerina purificada) e quatro repetições de dez aves. Foram avaliados o consumo de ração, ganho de peso, conversão alimentar, peso final, biometria dos órgãos (coração, fígado, moela e intestino delgado), rendimento de carcaça, rendimentos de cortes nobres (coxa, sobrecoxa e peito), coloração e a deposição de proteína e deposição de gordura da carne do peito. Os níveis de inclusão de glicerina purificada nas dietas influenciaram (p < 0,05) o ganho de peso, a conversão alimentar e o peso das aves aos 42 dias, não havendo efeito (p > 0,05) sobre o consumo de ração. Da mesma forma, não houve efeito (p > 0,05) sobre os rendimentos de carcaça e cortes nobres. Os níveis de inclusão de glicerina purificada não influenciaram (p > 0,05) os pesos relativos da moela, nem o peso relativo e/ou comprimento do intestino delgado. Entretanto, observou-se efeito (p < 0,05) sobre o peso relativo do coração e fígado. Os níveis de inclusão de glicerina purificada nas dietas não afetaram (p > 0,05) os valores de luminosidade (L*), vermelho (a*), amarelo (b*), pH e a deposição de proteína, mas, aumentou de maneira linear (p < 0,05) a deposição de gordura da carne de peito. A inclusão de até 6% de glicerina purificada nas dietas mostrou-se tecnicamente viável para frangos de corte dos 8 aos 42 dias de idade.(AU)

Purified glycerin in balanced diets of broiler chickens treated from 1 to 42 days of age

We evaluated the technical feasibility of purified glycerin inclusion in balanced diets of broiler chickens treated from 1 to 42 days of age. A total of 240 broiler chickens were distributed in a completely randomized design into four treatments (0, 2, 4, and 6% purified glycerin inclusion), with six replicates of 10 broilers each. We evaluated productive performance (at 7, 21, and 42 days), edible viscera (heart, liver, and gizzard), carcass yield and cuts, color, chemical and physical composition, as well as protein and fat deposition in the breast muscle. Dietary inclusion of purified glycerin reduced feed conversion and increased feed intake, weight gain, and weight at seven days. From 1 to 21 days, there was a decrease in feed conversion and a linear increase in weight gain, with no effect on feed intake. Considering the total experimental period, increasing glycerin levels increased weight gain and weight at 42 days, with no effects on feed conversion or feed intake. Similarly, there was no effect on carcass and cut yields, liver and gizzard yields, and weight or length of the intestine, while heart yield was decreased. There was a linear increase in crude protein, ether extract, protein, and fat deposition and a reduction in moisture percentages, with no effect on ash content. Purified glycerin levels did not affect a*, b*, L*, pH, temperature, shear force, or weight loss by cooking in breast meat. The dietary inclusion of purified glycerin of up to 6% proved to be technically feasible in the diets of broilers from 1 to 42 days.(AU)

Purified glycerin diets for broilers from 8 to 42 days old / Glicerina purificada em dietas para frangos de corte dos 8 aos 42 dias de idade

Here we evaluate the technical viability of including purified glycerin in balanced diets for broilers from 8 to 42 days old. For this, we used 160 8-day-old broiler chickens from the Cobb 500® lineage, distributed in a completely randomized design (CRD) with four treatments (0, 2, 4, and 6% of purified glycerin inclusion) and four replicates of ten birds. We evaluated feed intake, weight gain, feed conversion, final weight, organs biometry (heart, liver, gizzard and small intestine), carcass yield, special cuts yields (thigh, drumstick, and breast), meat color, and protein and fat deposition in the breast muscle. The purified glycerin inclusion levels in diets influenced (p 0.05) on feed intake (FI). Similarly, there was no effect (p > 0.05) on carcass and special cuts yields. The purified glycerin inclusion levels did not influence (p > 0.05) the relative gizzard weight, nor the relative small intestine weight and length. However, the purified glycerin inclusion levels did affect the relative heart and liver weights (p 0.05) lightness (L*), redness (a*), yellowness (b*) values, pH, or protein deposition, but did increases (p < 0.05) fat deposition in breast muscle. Based on our findings, we propose that the inclusion of 6% purified glycerin in diets is technically feasible for broilers of 8 to 42 days old.

Objetivou-se neste trabalho avaliar a viabilidade técnica da inclusão de glicerina purificada em dietas balanceadas para frangos de corte, dos 8 aos 42 dias de idade. Foram utilizados 160 pintos de corte, com 8 dias, da linhagem Cobb 500®, distribuídos em delineamento experimental inteiramente casualizado (DIC), com quatro tratamentos (0, 2, 4 e 6% de inclusão de glicerina purificada) e quatro repetições de dez aves. Foram avaliados o consumo de ração, ganho de peso, conversão alimentar, peso final, biometria dos órgãos (coração, fígado, moela e intestino delgado), rendimento de carcaça, rendimentos de cortes nobres (coxa, sobrecoxa e peito), coloração e a deposição de proteína e deposição de gordura da carne do peito. Os níveis de inclusão de glicerina purificada nas dietas influenciaram (p 0,05) sobre o consumo de ração. Da mesma forma, não houve efeito (p > 0,05) sobre os rendimentos de carcaça e cortes nobres. Os níveis de inclusão de glicerina purificada não influenciaram (p > 0,05) os pesos relativos da moela, nem o peso relativo e/ou comprimento do intestino delgado. Entretanto, observou-se efeito (p 0,05) os valores de luminosidade (L*), vermelho (a*), amarelo (b*), pH e a deposição de proteína, mas, aumentou de maneira linear (p < 0,05) a deposição de gordura da carne de peito. A inclusão de até 6% de glicerina purificada nas dietas mostrou-se tecnicamente viável para frangos de corte dos 8 aos 42 dias de idade.

Cassava bagasse and annatto colorific (Bixa orellana L. ) in diets for slow-growing broilers from 30 to 90 days of age / Bagaço de mandioca e colorífico de urucum (Bixa orellana L.) em dietas para frangos de crescimento lento de 30 a 90 dias de idade

The performance and breast skin color of slow-growing broilers from 30 to 90 days old fed diets with different inclusions of cassava bagasse and annatto colorific were evaluated. 308 slow-growing broilers were used in a completely randomized experimental design, in a factorial scheme with an additional treatment 3 X 2 + 1, with three levels of annatto colorific as a pigmentant agent (1%, 2% and 3%), two levels of cassava bagasse (10% and 20%) and an additional treatment (control diet), totaling seven treatments, four replicates and 11 broilers per experimental unit. Feed intake and feed conversion were affected by treatments, with interaction. The weight gain and final weight were influenced by the treatments and the best results were found for the inclusion of 10% cassava bagasse and 2% annatto colorific. There was no effect of treatment and no interaction on carcass yield. The color parameters L*, a* and b* were not influenced by treatments. The inclusion of 10% cassava bagasse and 1% annatto colorific for slow-growing broilers from 30 to 90 days of age is recommended. However, the use of these ingredients proved to be economically unfeasible.

Foram avaliados o desempenho e a cor da pele de frangos de corte de crescimento lento no período de 30 a 90 dias, alimentados com dietas com diferentes inclusões de bagaço de mandioca e colorífico de urucum. 308 frangos de corte de crescimento lento foram utilizados em um delineamento experimental inteiramente casualizado, em esquema fatorial com tratamento adicional 3 X 2 + 1, sendo três níveis de colorífico de urucum como agente pigmentante (1%, 2% e 3%), dois níveis de bagaço de mandioca (10% e 20%) e um tratamento adicional (dieta de controle), totalizando sete tratamentos, quatro repetições e 11 frangos de corte por unidade experimental. O consumo de ração e a conversão alimentar foram influenciados pelos tratamentos, com interação. O ganho de peso e o peso final foram influenciados pelos tratamentos (p<0,001) e os melhores resultados foram encontrados para a inclusão de 10% de bagaço de mandioca e 2% de colorífico de urucum. Não houve efeito dos tratamento e nenhuma interação sobre o rendimento da carcaça. Os parâmetros de cor L *, a * e b * não foram influenciados pelos tratamentos. É recomendável a inclusão de bagaço de mandioca a 10% e 1% de colorífico de urucum para frangos de crescimento lento de 30 a 90 dias de idade. No entanto, o uso desses ingredientes provou ser economicamente inviável.

Cassava bagasse and annatto colorific (Bixa orellana L. ) in diets for slow-growing broilers from 30 to 90 days of age / Bagaço de mandioca e colorífico de urucum (Bixa orellana L.) em dietas para frangos de crescimento lento de 30 a 90 dias de idade

The performance and breast skin color of slow-growing broilers from 30 to 90 days old fed diets with different inclusions of cassava bagasse and annatto colorific were evaluated. 308 slow-growing broilers were used in a completely randomized experimental design, in a factorial scheme with an additional treatment 3 X 2 + 1, with three levels of annatto colorific as a pigmentant agent (1%, 2% and 3%), two levels of cassava bagasse (10% and 20%) and an additional treatment (control diet), totaling seven treatments, four replicates and 11 broilers per experimental unit. Feed intake and feed conversion were affected by treatments, with interaction. The weight gain and final weight were influenced by the treatments and the best results were found for the inclusion of 10% cassava bagasse and 2% annatto colorific. There was no effect of treatment and no interaction on carcass yield. The color parameters L*, a* and b* were not influenced by treatments. The inclusion of 10% cassava bagasse and 1% annatto colorific for slow-growing broilers from 30 to 90 days of age is recommended. However, the use of these ingredients proved to be economically unfeasible.(AU)

Foram avaliados o desempenho e a cor da pele de frangos de corte de crescimento lento no período de 30 a 90 dias, alimentados com dietas com diferentes inclusões de bagaço de mandioca e colorífico de urucum. 308 frangos de corte de crescimento lento foram utilizados em um delineamento experimental inteiramente casualizado, em esquema fatorial com tratamento adicional 3 X 2 + 1, sendo três níveis de colorífico de urucum como agente pigmentante (1%, 2% e 3%), dois níveis de bagaço de mandioca (10% e 20%) e um tratamento adicional (dieta de controle), totalizando sete tratamentos, quatro repetições e 11 frangos de corte por unidade experimental. O consumo de ração e a conversão alimentar foram influenciados pelos tratamentos, com interação. O ganho de peso e o peso final foram influenciados pelos tratamentos (p<0,001) e os melhores resultados foram encontrados para a inclusão de 10% de bagaço de mandioca e 2% de colorífico de urucum. Não houve efeito dos tratamento e nenhuma interação sobre o rendimento da carcaça. Os parâmetros de cor L *, a * e b * não foram influenciados pelos tratamentos. É recomendável a inclusão de bagaço de mandioca a 10% e 1% de colorífico de urucum para frangos de crescimento lento de 30 a 90 dias de idade. No entanto, o uso desses ingredientes provou ser economicamente inviável.(AU)

Variability in neurocognitive performance: Age, gender, and school-related differences in children and from ages 6 to 12.

Cognitive development in children presents peculiarities according to groups of age, gender, and type of school. Few studies have been investigating the effects of all these factors. The aim of this study was to investigate the main effects and the interactions of age, gender, and type of school in 419 children from ages 6 to 12 years old evaluated by the Child Brief Neuropsychological Assessment Battery (NEUPSILIN-Inf). Older children, children in private schools and girls presented better results. Interactions between all three independent variables were observed in different cognitive domains. The results highlight both the heterogeneity and the influence of multiple factors in children's neuropsychological development.

Comparative effects of the ω3 polyunsaturated fatty acid derivatives resolvins E1 and D1 and protectin DX in models of inflammation and pain.

PURPOSE: Specialized pro-resolving lipid mediators (SPMs), also known as lipoxins, resolvins (Rvs), protectins and maresins, have been implicated in the resolution of the inflammatory process. However, a systematic comparison of their activity in the relief of inflammation and pain models is still lacking. MATERIALS AND METHODS: The effects of Rvs E1 and D1 and protectin DX (PDX) were assessed in rat paws inflamed by the standard proinflammatory stimulus carrageenan or by histamine, 5-hydroxytryptamine, substance P or prostaglandin E2. The experimental outcomes were the mechanical nociceptive threshold and increase in paw volume as a measure of pain and edema formation, respectively. The analgesic and anti-inflammatory activities of the indicated SPMs were also compared with nonsteroidal (indomethacin and celecoxib) and steroidal (dexamethasone) anti-inflammatory drugs. RESULTS: Only RvE1 and RvD1 presented analgesic and anti-inflammatory activities in the carrageenan model, and RvE1 was twice as potent as RvD1. Both substances tended to be better analgesics than anti-inflammatory agents, with a modeling profile similar to steroidal anti-inflammatory drugs. However, proinflammatory effects (edema formation) were also detected when the mediators histamine, 5-hydroxytryptamine or substance P replaced carrageenan as the proinflammatory stimuli. The analgesic and anti-inflammatory effects of resolvins were specifically prevented by an antagonist of the leukotriene B4 receptor 1 (BLT1). CONCLUSION: Rvs, as analgesic agents, may be better therapeutic agents than nonsteroidal anti-inflammatory drugs, the current choice in the relief of pain of an inflammatory origin. However, the possibility of developing adverse effects cannot be overlooked.

Effects of purified glycerin in balanced diets of chicken broilers treated from 22 to 42 days of age1 / Glicerina purificada em dietas balanceadas para frangos de corte dos 22 aos 42 dias de idade

The aim of this study was to investigate the effects of including purified glycerin in the diets of broilers from 22 to 42 days of age. Eighty Cobb 500® lineage broilers were distributed in a completely randomized design (CRD) to one of four treatment groups (0, 2, 4 and 6% of purified glycerin inclusion in feed) and five replicates of four birds. The birds were evaluated for performance (weight gain, feed intake and feed conversion); carcass yield; special cuts yield (thigh, drumstick and breast); muscle colour (L* = lightness, a* = redness, b* = yellowness); and crude protein, ether extract and moisture content of the breast muscle. Inclusion of purified glycerin in the diet did not affect (p > 0.05) any of the growth performance indicators, which demonstrated the technical feasibility of including up to 6% glycerin in the diet. Similarly, there was no effect on carcass yield; special-cut yields; muscle colour (L* = lightness, a* = redness, b* = yellowness); or crude protein, ether extract and moisture content of breast muscle. The inclusion of purified glycerin at up to 6% in the diet proved to be feasible in broilers treated from 22 to 42 days of age.(AU)

Objetivou-se neste trabalho avaliar os efeitos da inclusão de glicerina purificada em dietas balanceadas para frangos de corte dos 22 aos 42 dias de idade. Foram utilizados 80 frangos de corte, da linhagem Cobb 500®, distribuídos em delineamento experimental inteiramente casualizado (DIC), com quatro tratamentos (0, 2, 4 e 6% de inclusão de glicerina purificada) e cinco repetições de quatro aves. Foram avaliados o desempenho (ganho de peso, consumo de ração e conversão alimentar), rendimentos de carcaça e cortes nobres (coxa, sobrecoxa e peito), a coloração (L* = Luminosidade, a* = teor de vermelho e b* = teor de amarelo) e os teores de proteína bruta, extrato etéreo e umidade da carne do peito. Os níveis de inclusão de glicerina purificada nas dietas não influenciaram (p > 0,05) nenhuma das características de desempenho, o que evidenciou a viabilidade técnica de inclusão de até 6% do alimento. Da mesma forma, não houve efeito sobre os rendimentos de carcaça e cortes nobres, coloração da carne (L* = Luminosidade, a* = teor de vermelho e b* = teor de amarelo) e nos teores de proteína bruta, extrato etéreo e umidade. A inclusão de glicerina purificada mostrou-se viável em até 6% em dietas balanceadas para frangos de corte dos 22 aos 42 dias de idade.(AU)