RESUMO
CONTEXT AND OBJECTIVE: The Epstein-Barr virus (EBV) is the most common cause of infectious mononucleosis and is also associated with several human tumors, including Burkitt's lymphoma, Hodgkin's lymphoma, some cases of gastric carcinoma and nasopharyngeal carcinoma, among other neoplasms. The aim of this study was to screen 75 primary gliomas for the presence of specific EBV DNA sequences by means of the polymerase chain reaction (PCR), with confirmation by direct sequencing. DESIGN AND SETTING: Prevalence study on EBV molecular genetics at a molecular pathology laboratory in a university hospital and at an applied genetics laboratory in a national institution. METHODS: A total of 75 primary glioma biopsies and 6 others from other tumors from the central nervous system were obtained. The tissues were immediately frozen for subsequent DNA extraction by means of traditional methods using proteinase K digestion and extraction with a phenol-chloroform-isoamyl alcohol mixture. DNA was precipitated with ethanol, resuspended in buffer and stored. The PCRs were carried out using primers for amplification of the EBV BamM region. Positive and negative controls were added to each reaction. The PCR products were used for direct sequencing for confirmation. RESULTS: The viral sequences were positive in 11/75 (14.7%) of our samples. CONCLUSION: The prevalence of EBV DNA was 11/75 (14.7%) in our glioma collection. Further molecular and epidemiological studies are needed to establish the possible role played by EBV in the tumorigenesis of gliomas.
Assuntos
Neoplasias Encefálicas/virologia , DNA Viral/genética , Glioma/virologia , Herpesvirus Humano 4/genética , Adolescente , Adulto , Astrocitoma/virologia , Criança , Endopeptidase K , Feminino , Humanos , Masculino , Reação em Cadeia da Polimerase , Análise de Sequência de DNA/métodosRESUMO
Members of the Herpesviridae family have been implicated in a number of tumours in humans. At least 75% of the human population has had contact with cytomegalovirus (HCMV). In this work, we screened 75 Brazilian glioma biopsies for the presence of HCMV DNA sequences. HCMV DNA was detected in 36% (27/75) of the biopsies. It is possible that HCMV could be a co-factor in the evolution of brain tumours.
Assuntos
Neoplasias Encefálicas/virologia , Infecções por Citomegalovirus/complicações , Citomegalovirus/genética , DNA Viral/análise , Glioma/virologia , Adulto , Biópsia , Criança , Estudos de Coortes , Citomegalovirus/imunologia , Infecções por Citomegalovirus/diagnóstico , Feminino , Humanos , Proteínas Imediatamente Precoces/análise , Proteínas Imediatamente Precoces/imunologia , Masculino , Estadiamento de Neoplasias , Reação em Cadeia da Polimerase , Prevalência , Adulto JovemRESUMO
Members of the Herpesviridae family have been implicated in a number of tumours in humans. At least 75% of the human population has had contact with cytomegalovirus (HCMV). In this work, we screened 75 Brazilian glioma biopsies for the presence of HCMV DNA sequences. HCMV DNA was detected in 36% (27/75) of the biopsies. It is possible that HCMV could be a co-factor in the evolution of brain tumours.
Assuntos
Adulto , Criança , Feminino , Humanos , Masculino , Adulto Jovem , Neoplasias Encefálicas/virologia , Infecções por Citomegalovirus/complicações , Citomegalovirus/genética , DNA Viral/análise , Glioma/virologia , Biópsia , Estudos de Coortes , Infecções por Citomegalovirus/diagnóstico , Citomegalovirus/imunologia , Proteínas Imediatamente Precoces/análise , Proteínas Imediatamente Precoces/imunologia , Estadiamento de Neoplasias , Reação em Cadeia da Polimerase , PrevalênciaRESUMO
Beare-Stevenson syndrome (BSS) (MIM#123790) is a rare disorder characterized by craniofacial anomalies and cutis gyrata associated with anogenital anomalies and prominent umbilical stump. There are few reports on the syndrome, and molecular analysis has revealed the involvement of two closely spaced mutations within the FGFR2 gene: c.1115Câ·G (p.S372C) and c.1124Câ·G (p.Y375C). We herein describe a new case of a c.1124Câ·G mutation in a BSS patient.
RESUMO
Gliomas of astrocytic origin are the most common primary brain tumors, accounting for over 40 to 50% of all central nervous system tumors. The TP53 tumor suppressor gene is the most frequently mutated gene found in human malignancies. A mutation of this gene can lead to an increased half-life of the resulting protein and loss of biological function. High levels of p53 have been detected in the serum of colon cancer patients, although p53 protein has not been detected in the serum of brain tumor patients. Besides circulating p53, several studies have detected antibodies against p53 in patients with lung and breast cancer, as well as those with other types of cancer. We studied p53 protein and anti-p53 antibodies in the plasma of Brazilian brain tumor patients. Plasma samples were drawn from 24 untreated brain tumor patients and from 15 healthy donors without clinical signs of cancer. Western blotting techniques were used to detect p53 protein and anti-p53 antibodies. We found anti-p53 antibodies in 5/24 brain tumor patients. Age appears to affect the immune response, as four of six tumor patients under 16 years old had detectable anti-p53 antibodies, while these were found in only 1 of 18 adults (over 16 years old). We found no p53 protein in any of the serum samples from the brain tumors. Possibly the presence of this protein is affected by tumor type or by the organs that are sampled.
